3-4-dihydroxyphenyllactic-acid and Scleroderma--Systemic

To study the effects of tanshinone IIA, danshensu, lithospermate, and protocatechualdehyde on proliferation and procollagen mRNA synthesis of dermal fibroblasts from patients with systemic sclerosis (SSc).. Dermal fibroblasts were obtained from 5 patients with SSc, 2 males and 3 females, and sex and age-matched 5 patients undergoing plastic surgery, cultured, and then co-cultured with tanshinone IIA, danshensu, lithospermate, and protocatechualdehyde respectively. MTT test was used to study the effects of the four constituents on fibroblasts cell proliferation. RT-PCR was used to detect the level of procollagen mRNA expression in the control fibroblasts and in the SSc fibroblasts before and after the addition of the four constituents.. The 4 constituents all suppressed the proliferation of fibroblasts from SSc patients, with tanshinone IIA being the most effective. The 4 constituents remarkably inhibited the mRNA expression of type I and III procollagen of the fibroblasts with tanshinone II A showing the most inhibition effect that reduced the expressions of procollagen I mRNA by 47% and procollagen III mRNA by 45%.. Of the four constituents, tanshinone IIA is the most suppressive effect on the cell proliferation and type I and type III procollagen synthesis of SSc fibroblasts, demonstrating that it has a more antifibrotic effect.

Topics: Abietanes; Benzaldehydes; Catechols; Cell Proliferation; Cells, Cultured; Collagen Type I; Collagen Type III; Drugs, Chinese Herbal; Female; Fibroblasts; Humans; Lactates; Male; Phenanthrenes; Plant Extracts; Procollagen; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Salvia miltiorrhiza; Scleroderma, Systemic; Transcription, Genetic