25-hydroxyvitamin-d-2 and Diabetes-Mellitus

It was done the prospective study of the examination results of 40 patients with diabetes mellitus. All patients were divided into 2 groups for study of 25-OH-vitamin D effect, cholesterol, triglycerides in blood serum on the nature of diabetes mellitus. The first group included patients with ulcero-necrotic injuries in case of neuro-ischemic forms of diabetic foot syndrome. The second group included patients without such injuries. It was significantly determined that there was more neuro-ischemic injuries in case of 25-OH-vitamin D level less than 30 nmol/l. Injuries are connected with reduction of arterial inflow because of osslusive-stenotic lesions of arteries. 25-OH-vitamin D level less than 30 nmol/l is a marker of neuro-ischemic injuries of foot in case of diabetes mellitus. This indication is more specific than cholesterol and triglycerides levels.

Topics: 25-Hydroxyvitamin D 2; Adult; Biomarkers; Cholesterol; Diabetes Mellitus; Diabetic Foot; Female; Humans; Ischemia; Male; Middle Aged; Prospective Studies; Reproducibility of Results; Sensitivity and Specificity; Statistics as Topic; Triglycerides

Now emerging as an important risk factor for type 1 diabetes, vitamin D deficiency is also associated with obesity, metabolic syndrome, and type 2 diabetes and has been identified as a potential cardiometabolic risk factor. A simple, accurate screening test for 25-hydroxy vitamin D [25(OH)D] deficiency is needed. We developed a liquid chromatography/tandem mass spectrometry assay for 25-hydroxy vitamin D(2) [25(OH)D(2)] and 25-hydroxy vitamin D(3) [25(OH)D(3)] in dried blood spots.. Blood spots were collected by finger stick simultaneously with serum samples obtained by venipuncture from healthy volunteers. Disks punched from the dried blood spots were sonicated with an internal standard solution of deuterated 25(OH)D(3) (26,26,26,27,27,27-d(6)). Methanol was added to precipitate proteins prior to extraction with hexane. The extracted samples were dried and reconstituted in 50:50 methanol:H(2)O before injection into a Varian 320-MS TQ mass spectrometer.. BLOOD SPOT ASSAY PRECISION WAS GOOD OVER THE REPORTABLE RANGE: interassay coefficients of variation were 13, 13, and 11% at concentrations of 14, 26, and 81 ng/ml, respectively, for 25-hydroxy vitamin D(3) and 12% at 23 ng/ml for 25(OH)D(2). The 25(OH)D(3) assay was linear from 3.5 to 75 ng/ml (R > 0.99). Blood spot and serum values showed excellent correlation for 25(OH)D(2) (R=0.90, n=54) and 25(OH)D(3) (R=0.91, n=83).. This blood spot assay for 25(OH)D(2) and 25(OH)D(3) provides a convenient and cost-effective alternative to serum assays and can be automated. This may be valuable in large-scale screening for risk of type 1 diabetes, for cardiometabolic risk screening, and for monitoring vitamin D supplementation.

Topics: 25-Hydroxyvitamin D 2; Calcifediol; Chromatography, Liquid; Diabetes Mellitus; Hematologic Tests; Humans; Mass Screening; Metabolic Syndrome; Risk Factors; Sensitivity and Specificity; Tandem Mass Spectrometry; Vitamin D Deficiency

In order to elucidate if changes in vitamin D metabolism play a role for diabetic bone loss, the serum concentrations of the major vitamin D metabolites were studied in 26 adult male ambulatory insulin-treated diabetics, selected to have normal renal function and a duration of diabetes below 11 years. The patients were studied during usual metabolic control and exhibited wide ranges of hyperglycaemia and glycosuria. The serum concentrations of the major metabolites of vitamin D, 25-hydroxyvitamin D(2 + 3) (25OHD), 24,25-dihydroxyvitamin D(2 + 3) (24,25(OH)2D), and 1,25-dihydroxyvitamin D(2 + 3) (1,25(OH)2D), were measured in diabetics, and in age and sex matched controls. The diabetics had slightly decreased serum levels of 25OHD (42.0 nmol/l versus 55.5 nmol/l in normals, P less than 0.05), markedly decreased serum levels of 24,25(OH)2D (2.98 nmol/l versus 5.91 nmol/l, P less than 0.01), but serum levels of 1,25(OH)2D were virtually normal (64.2 pmol/l versus 68.3 pmol/l, ns). The close correlation between serum concentrations of 25OHD and 24,25(OH)2D observed in the normal subjects, was absent in the diabetics. There were no correlations between the serum levels of any of the vitamin D metabolites and the measured indices of glucose and calcium metabolism. It is concluded that insulin-dependent diabetic patients demonstrate definite alterations in serum levels of vitamin D metabolites, the significance of which remains unknown at present.

Topics: 24,25-Dihydroxyvitamin D 3; 25-Hydroxyvitamin D 2; Adult; Calcifediol; Calcitriol; Diabetes Mellitus; Dihydroxycholecalciferols; Ergocalciferols; Humans; Insulin; Male; Middle Aged; Vitamin D