24-24-difluoro-1-25-dihydroxyvitamin-d3 and Hypertension

Intestinal calcium transport rate and response to treatment with a vitamin D agonist [24,24-difluoro-1,25-dihydroxycholecalciferol, F2-1,25-(OH)2D3)] were studied in the spontaneously hypertensive (SH) rat-Wistar Kyoto (WKy) rat model of hypertension. We used the everted duodenal sac to study untreated, orally treated, and parenterally treated groups of SH and WKy rats. In untreated groups, net calcium transport was lower (P less than 0.05) in the SH rat than in the WKy rat (0.46-0.66 vs 0.81-1.04, all data mumole/g segment wet wt per hr). Both groups responded to treatment (SH vs WKy; 0.84-0.90 vs 1.56-1.57, P less than 0.05), but even in treated groups net calcium transport by the SH rat was lower than that by the WKy rat (P less than 0.05). Net water transport increased 3- to 8-fold in response to treatment in the WKy but not in the SH rat. The increased water transport prevented demonstration of treatment-mediated increased calcium transport based on serosal/mucosal concentration ratio in the WKy rat. We conclude that (i) both the SH and the WKy rat have the capability to increase calcium transport when treated with an agonist having vitamin D activity; (ii) the unstimulated and stimulated transport rate is lower in the SH rat than in the WKy rat; and (iii) water transport responds to treatment in the WKy rat but not in the SH rat.

Topics: Animals; Biological Transport; Body Water; Calcitriol; Calcium; Hypertension; Magnesium; Male; Rats; Rats, Inbred SHR; Rats, Inbred WKY