2-fluorohistidine and Body-Weight

The tissue distribution and time course of incorporation into acid insoluble (bound) and acid soluble (free) fractions of [3H]2-fluorohistidine is compared to that of U[14C]Histidine in mouse tissues in vivo. The cycloheximide-sensitive incorporation of 2-FHis is between 9 and 17 percent of that of His. Unlike [14C]His a major fraction, approximately 90% at 72 hrs, of isotope derived from [3H]2-FHis remains in tissues for a prolonged period in an acid soluble form. The excretion of isotope derived from [14C]His (T1/2 = 5 hr) is more rapid than from [3H]2-FHis (T1/2 = 11.4 hrs). 2-FHis, at doses from 100 to 250 mg/kg produce a reversible inhibition of growth in mice.

Topics: Animals; Body Weight; Brain; Carbon Radioisotopes; Cycloheximide; Dose-Response Relationship, Drug; Histidine; Kidney; Leucine; Liver; Male; Mice; Mice, Inbred BALB C; Proteins; Sensitivity and Specificity; Solubility; Tissue Distribution; Tritium