2-4-dinitrophenylhydrazine and Carcinoma--Ehrlich-Tumor

2-4-dinitrophenylhydrazine has been researched along with Carcinoma--Ehrlich-Tumor* in 1 studies

Other Studies

1 other study(ies) available for 2-4-dinitrophenylhydrazine and Carcinoma--Ehrlich-Tumor

Article	Year
Quantification of the histochemical staining for carbonyles and DNA using 3-hydroxy-2-naphthoic acid hydrazide and fast blue B. Histochemistry, 1990, Volume: 94, Issue:5 Fixed cells and tissues pretreated with 4-hydroxynonenal were used as models for the histochemical demonstration of protein bound aldehydic groups. The aldehydes were stained with both a modification of the 2,4-dinitrophenylhydrazine method (2,4-DNPH) and the optimized staining using 3-hydroxy-2-naphthoic acid hydrazide and Fast blue B (NAH-FB). A correlation has been found between the specific microphotometric mean integrated maximum absorbance values of cells and tissues stained with 2,4-DNPH and with NAH-FB (cc = 0.999). The maximum absorbance measured after 2,4-DNPH-staining (epsilon 367 = 21,000) were 1.893 +/- 0.072 (P less than 0.01) times that of NAH-FB-staining at 550 nm. Microphotometrically determined DNA-values of different cells stained with the NAH-FB-DNA-method correlated with those determined with methods of analytical biochemistry and published by other authors. Topics: Aldehydes; Animals; Carcinoma, Ehrlich Tumor; Diazonium Compounds; DNA; Histocytochemistry; Liver; Microchemistry; Naphthols; Phenylhydrazines; Photometry; Proteins; Rats	1990

Article

Year

Quantification of the histochemical staining for carbonyles and DNA using 3-hydroxy-2-naphthoic acid hydrazide and fast blue B.

Histochemistry, 1990, Volume: 94, Issue:5

Fixed cells and tissues pretreated with 4-hydroxynonenal were used as models for the histochemical demonstration of protein bound aldehydic groups. The aldehydes were stained with both a modification of the 2,4-dinitrophenylhydrazine method (2,4-DNPH) and the optimized staining using 3-hydroxy-2-naphthoic acid hydrazide and Fast blue B (NAH-FB). A correlation has been found between the specific microphotometric mean integrated maximum absorbance values of cells and tissues stained with 2,4-DNPH and with NAH-FB (cc = 0.999). The maximum absorbance measured after 2,4-DNPH-staining (epsilon 367 = 21,000) were 1.893 +/- 0.072 (P less than 0.01) times that of NAH-FB-staining at 550 nm. Microphotometrically determined DNA-values of different cells stained with the NAH-FB-DNA-method correlated with those determined with methods of analytical biochemistry and published by other authors.

Topics: Aldehydes; Animals; Carcinoma, Ehrlich Tumor; Diazonium Compounds; DNA; Histocytochemistry; Liver; Microchemistry; Naphthols; Phenylhydrazines; Photometry; Proteins; Rats

1990