2-4-dinitrophenylhydrazine and Burns

2-4-dinitrophenylhydrazine has been researched along with Burns* in 1 studies

Other Studies

1 other study(ies) available for 2-4-dinitrophenylhydrazine and Burns

Article	Year
Oxidatively modified plasma phospholipids containing reactive carbonyl functions measured by HPLC: evidence for phosphatidylcholine-bound aldehydes in plasma of burn patients. Free radical research, 1995, Volume: 22, Issue:4 A HPLC method has been developed to measure phosphatidylcholine (PC) containing reactive carbonyl functions in the sn-acyl residue in order to study processes in which such reactive carbonyls can be formed due to e.g. oxidative fragmentation. The method has been applied to determine PC-bound carbonyls as 2,4-dinitrophenylhydrazones (DNPH) in plasma of burn patients. Plasma from healthy volunteers served as controls. Additionally, in vitro oxidation experiments (A: plasma, buffer diluted; B: plasma + iron-EDTA complex and C: plasma + iron-EDTA complex + H2O2) have been performed to obtain and to identify 2,4-dinitrophenylhydrazine derivatizable carbonyl functions in plasma PC. Both, the PC-aldehydes and PC-aldehyde DNPH derivatives were cleavable with phospholipase C. Quantification was based on thin-layer chromatography purified soybean phosphatidylcholine, which was identically oxidized and derivatized as the plasma lipids in vitro. Topics: Adult; Aldehydes; Burns; Chromatography, High Pressure Liquid; Edetic Acid; Female; Glycine max; Humans; Hydrogen Peroxide; Iron Chelating Agents; Male; Middle Aged; Oxidation-Reduction; Phenylhydrazines; Phosphatidylcholines; Spectrophotometry	1995

Article

Year

Oxidatively modified plasma phospholipids containing reactive carbonyl functions measured by HPLC: evidence for phosphatidylcholine-bound aldehydes in plasma of burn patients.

Free radical research, 1995, Volume: 22, Issue:4

A HPLC method has been developed to measure phosphatidylcholine (PC) containing reactive carbonyl functions in the sn-acyl residue in order to study processes in which such reactive carbonyls can be formed due to e.g. oxidative fragmentation. The method has been applied to determine PC-bound carbonyls as 2,4-dinitrophenylhydrazones (DNPH) in plasma of burn patients. Plasma from healthy volunteers served as controls. Additionally, in vitro oxidation experiments (A: plasma, buffer diluted; B: plasma + iron-EDTA complex and C: plasma + iron-EDTA complex + H2O2) have been performed to obtain and to identify 2,4-dinitrophenylhydrazine derivatizable carbonyl functions in plasma PC. Both, the PC-aldehydes and PC-aldehyde DNPH derivatives were cleavable with phospholipase C. Quantification was based on thin-layer chromatography purified soybean phosphatidylcholine, which was identically oxidized and derivatized as the plasma lipids in vitro.

Topics: Adult; Aldehydes; Burns; Chromatography, High Pressure Liquid; Edetic Acid; Female; Glycine max; Humans; Hydrogen Peroxide; Iron Chelating Agents; Male; Middle Aged; Oxidation-Reduction; Phenylhydrazines; Phosphatidylcholines; Spectrophotometry

1995