2-4-dinitrofluorobenzene-sulfonic-acid and Acute-Disease

There is a close relationship between inflammatory bowel disease (IBD) and various hepatobiliary disorders. The objective of this study was to determine whether hepatic leukocyte recruitment occurs in experimental colitis. We used the murine model of colitis induced by 2,4-dinitrobenezenesulfonic acid (DNBS). Male C57Bl/6 mice received an intrarectal injection of 4 mg DNBS in 100 microl 50% ethanol. Controls received 100 microl 50% ethanol. The hepatic microcirculation was examined at 3 and 14 days post-DNBS by intravital video microscopy. Three days post-DNBS, when mice had developed acute colitis, there was associated hepatic leukocyte recruitment. Within the postsinusoidal venules there was a fourfold increase in the flux of rolling leukocytes that was P-selectin dependent but not alpha(4)-integrin dependent. There was also an increase in stationary leukocytes within the sinusoids, although this was not associated with an increase in serum alanine transaminase. By 14 days post-DNBS when macroscopic evidence of colonic inflammation was resolved, rolling within the postsinusoidal venules had returned to control levels. In this murine model of colitis, we describe a link between acute colonic inflammation and remote hepatic leukocyte recruitment that is P-selectin dependent. Active IBD may lead to remote hepatic inflammation.

Topics: Acute Disease; Administration, Rectal; Animals; Antigens, CD; Bile Ducts; Cell Adhesion; Cell Movement; Colitis; Dinitrofluorobenzene; Integrin alpha4; Leukocytes; Liver; Male; Mice; Mice, Inbred C57BL; P-Selectin; Time Factors; Venules

Interleukin-17 (IL-17) is a proinflammatory cytokine produced by T cells. The involvement of IL-17 in human diseases has been suspected because of its detection in sera from asthmatic patients and synovial fluids from arthritic patients. In this study, we generated IL-17-deficient mice and investigated the role of IL-17 in various disease models. We found that contact, delayed-type, and airway hypersensitivity responses, as well as T-dependent antibody production, were significantly reduced in the mutant mice, while IL-17 deficiency of donor T cells did not affect acute graft-versus-host reaction. The results suggest that impaired responses were caused by the defects of allergen-specific T cell activation. Our findings indicate that IL-17 plays an important role in activating T cells in allergen-specific T cell-mediated immune responses.

Topics: Acute Disease; Animals; Antibody Formation; B-Lymphocytes; Bronchial Hyperreactivity; CD4-Positive T-Lymphocytes; Cells, Cultured; Coculture Techniques; Dendritic Cells; Dermatitis, Allergic Contact; Dinitrofluorobenzene; Female; Graft vs Host Reaction; Haptens; Hypersensitivity, Delayed; Immunity, Cellular; Interleukin-17; Lymphocyte Activation; Lymphocyte Cooperation; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Knockout; Mitogens; Models, Animal; Nickel; Picryl Chloride; Specific Pathogen-Free Organisms; Spleen; T-Lymphocyte Subsets; Tumor Necrosis Factor-alpha