2-3-5-4--tetrahydroxystilbene-2-o-glucopyranoside and Brain-Ischemia

Topics: Angiogenesis Inducing Agents; Angiotensin I; Animals; Blotting, Western; Brain Injuries; Brain Ischemia; Fallopia multiflora; Glucosides; Infarction, Middle Cerebral Artery; Male; Neovascularization, Physiologic; Neuroprotective Agents; Platelet Endothelial Cell Adhesion Molecule-1; Rats; Rats, Sprague-Dawley; Receptor, TIE-2; Reperfusion Injury; Stilbenes; Stroke; Vascular Endothelial Growth Factor A

To investigate the effects of terahydroxy stilbene glucoside (TSG) on neurological deficits, the expressions of nerve growth factor (NGF) and growth associated protein43 (GAP43) in rats after Cerebral Ischemia-reperfusion.. 96 Sprague-Dawley male rats were divided into four groups (n = 24): control group, ischemia-reperfusion (I/R) model group, low dose TSG (60 mg/kg) group and high dose TSG (120 mg/kg) group. After 6 days' administration of TSG or natural saline (model group), reversible middle cerebral artery occlusion (MCAO) model was established by intraluminal suture technique. Rats in control group were operated while middle cerebral artery were not blocked. At 6, 24, 48 h and 7 d after reperfusion, behavior test was used to evaluate the neurological deficiency of each group. The expressions of NGF and GAP-43 in the cortex were measured by immunohistochemical method.. Compared with model group, both dose of TSG could decrease the grade of the rat neurological defects except at 6 h of ter reperfusion and increase the protein expressions of NGF and GAP-43 after reperfusion.. TSG can improve the neurological function through increasing the expressions of NGF and GAP-43 of cerebral ischemia-reperfusion rats.

Topics: Animals; Brain Ischemia; Cerebral Cortex; Disease Models, Animal; GAP-43 Protein; Glucosides; Immunohistochemistry; Infarction, Middle Cerebral Artery; Male; Nerve Growth Factor; Neuroprotective Agents; Polygonaceae; Random Allocation; Rats; Rats, Sprague-Dawley; Reperfusion Injury; Stilbenes