2-2--azino-di-(3-ethylbenzothiazoline)-6-sulfonic-acid and Periodontal-Pocket

The aim of this study was to determine the effect of non-surgical periodontal treatment on gingival crevicular fluid (GCF) and serum oxidant-antioxidant levels in smoking and non-smoking patients with chronic periodontitis.. Twenty-nine patients with chronic periodontitis (15 smokers (CP-S) and 14 non-smokers (CP-NS)) and 20 periodontally healthy subjects (10 smokers (H-S) and 10 non-smokers (H-NS)) totalling 49 subjects were included in this study. GCF was collected from at least two pre-selected sites (one moderate and one deep pocket) in patients with CP. In the healthy group, GCF samples were collected from one site. Probing pocket depth, clinical attachment level (CAL), gingival and plaque indices, and bleeding on probing were measured. To determine serum total oxidant status (TOS) and total antioxidant status (TAS), venous blood was drawn from each subject. The GCF, serum sampling, and clinical measurements were recorded at baseline and 6 weeks after periodontal treatment.. The study showed statistically significant improvement of clinical parameters after periodontal treatment in both smokers and non-smokers. In the CP-S group, there were no significant differences in GCF TAS levels at both moderate and deep pocket sites between baseline and 6 weeks (p>0.05). GCF TAS levels in the CP-NS groups were significantly increased (p<0.05) at moderate and deep pocket sites between baseline and 6 weeks. GCF TOS levels in the CP-S groups were significantly decreased (p<0.05) at deep pocket sites between baseline and 6 weeks. There was no significant difference in serum TAS levels of the all periodontitis patient groups between at baseline and 6 weeks (p>0.05). Serum TOS levels in the CP-S and CP-NS groups were significantly decreased (p<0.05) after periodontal treatments.. The periodontal treatment improves the clinical parameters in both smokers and non-smokers. These results confirm that non-surgical periodontal therapy can reduce oxidative stress.

Topics: Adult; Antioxidants; Benzothiazoles; Chronic Periodontitis; Dental Plaque Index; Dental Scaling; Female; Fluorescent Dyes; Follow-Up Studies; Gingival Crevicular Fluid; Humans; Indicators and Reagents; Male; Oral Hygiene; Oxidants; Oxidative Stress; Patient Education as Topic; Periodontal Attachment Loss; Periodontal Index; Periodontal Pocket; Phenols; Root Planing; Smoking; Spectrophotometry; Sulfonic Acids; Sulfoxides

The aim of this study was to evaluate the impact of smoking on the relationship between interleukin-1 (IL-1β) and oxidation in patients with periodontitis and response to nonsurgical periodontal therapy.. Data were obtained from 30 patients with generalized chronic periodontitis (15 smokers and 15 nonsmokers) and from 10 periodontally healthy controls. IL-1β level, total oxidant status (TOS) and total antioxidant status (TAS) were recorded in gingival crevicular fluid. Probing depth, clinical attachment level, gingival and plaque indices and bleeding on probing were also measured. The gingival crevicular fluid and clinical parameters were recorded at baseline and 6 wk after periodontal treatment.. The study showed statistically significant improvement of clinical parameters in both smokers and nonsmokers after periodontal treatment. Moreover, the baseline IL-1β levels were significantly higher in smokers compared with nonsmokers (p < 0.05). After periodontal treatment, the IL-1β levels were significantly reduced in both smokers and nonsmokers (p < 0.05). There were no significant differences in TOS and TAS between periodontitis patients and healthy controls at baseline and 6 wk after periodontal treatment. The level of IL-1β in gingival crevicular fluid was positively correlated with TOS in both smokers and nonsmokers.. Periodontal treatment improved the clinical parameters in both smokers and nonsmokers. The results confirm that periodontal therapy has an effect on IL-1β levels in gingival crevicular fluid, but not on TOS and TAS.

Topics: Adult; Antioxidants; Benzothiazoles; Chromogenic Compounds; Chronic Periodontitis; Colorimetry; Dental Plaque Index; Dental Scaling; Dianisidine; Female; Fluorescent Dyes; Follow-Up Studies; Gingival Crevicular Fluid; Gingival Hemorrhage; Humans; Indicators and Reagents; Interleukin-1beta; Male; Oral Hygiene; Oxidants; Oxidation-Reduction; Periodontal Attachment Loss; Periodontal Index; Periodontal Pocket; Phenols; Root Planing; Smoking; Sulfonic Acids; Sulfoxides