2-2--azino-di-(3-ethylbenzothiazoline)-6-sulfonic-acid and Hyperpigmentation

Melanin is a dark pigment produced by melanocytes. Tyrosinase is a key enzyme which catalyzes the rate-limiting step of melanogenesis. However, accumulation of melanin leads to various skin hyperpigmentation disorders. To find a novel skin-whitening agent, the antioxidant capacity of Bifidobacterium adolescentis culture filtrate and inhibitory effect on melanogenesis were investigated. The antioxidant effects of B. adolescentis culture filtrate include 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging capacity, 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid)(ABTS) radical cation scavenging activity and reducing power were measured spectrophotometrically. The reducing power is a useful index for the evaluation of potential antioxidants which carry out reduction of ferricyanide to ferrocyanide. Furthermore, the inhibitory effects of the bacterial culture filtrate on mushroom tyrosinase, B16F10 intracellular tyrosinase activity and melanin content were also determined. The results revealed that B. adolescentis culture filtrate (2.5, 5.0 and 7.5 %; v/v) effectively scavenged DPPH and ABTS radicals, and lower concentrations of the bacterial culture filtrates (0.5, 1.0 and 1.5 %; v/v) showed potent reducing power in a dose-dependent pattern. Additionally, the bacterial culture filtrate suppressed murine tyrosinase activity and decreased the amount of melanin in a dose-dependent manner. Our results demonstrated that B. adolescentis culture filtrate decreases the melanogenesis process of melanoma cells by inhibiting tyrosinase activity, which we suggest may be mediated through its antioxidant activity.

Topics: Animals; Antioxidants; Benzothiazoles; Bifidobacterium; Biphenyl Compounds; Cell Line, Tumor; Cell Proliferation; Cell Survival; Cells, Cultured; Hyperpigmentation; Melanins; Melanocytes; Melanoma, Experimental; Mice; Monophenol Monooxygenase; Picrates; Regression Analysis; Sulfonic Acids

Various skin hyperpigmentation disorders consist in accumulation and overproduction of melanin. In this report, we investigated the melanogenesis inhibitory and antioxidant effects of Bifidobacterium bifidum culture filtrate. The results revealed that B. bifidum culture filtrate effectively suppresses murine tyrosinase activity and decreases the amount of intracellular melanin in a dose-dependent manner. Additionally, the bacterial culture filtrate-scavenged DPPH and ABTS radicals, and shows potent-reducing power in a dose-dependent pattern. Our results expand the application of B. bifidum culture filtrate in the development and research of skin-whitening ingredients.

Topics: Agaricales; Animals; Antioxidants; Benzothiazoles; Bifidobacterium; Biphenyl Compounds; Cells, Cultured; Chick Embryo; Culture Media, Conditioned; Enzyme Activation; Fungal Proteins; Hyperpigmentation; Melanins; Mice; Monophenol Monooxygenase; Picrates; Sulfonic Acids