2-2--azino-di-(3-ethylbenzothiazoline)-6-sulfonic-acid and Breast-Neoplasms

Exosomes are membrane-enclosed phospholipid extracellular vesicles with a variety of tumor antigens which can be applied in the diagnosis and treatment of cancer due to the high secretion on the surface of cancer cells. Until now, many research studies on exosomes have been reported, but convenient and low-cost detection methods still need to be developed. Recently, we have developed a sensitive, simple and low-cost colorimetric aptasensor by designing a hairpin-like structure, which combined the highly specific MUC1 aptamer with a hemin/G-quadruplex for the detection of breast cancer exosomes. The hemin/G-quadruplex toward H2O2 reduction was used to generate an evidently strong colorimetric response owing to acting as a HRP-mimicking DNAzyme. The aptasensor is regarded as an "on-off" type switch, which strictly controls the process of reaction responding to the existence or not of exosomes. In our study, associated exosome detection limits are 3.94 × 105 particles per mL, which showed a higher sensitivity compared to commercial ELISA. Our method not only exhibited the advantages of being convenient and time-saving, with few instruments used, but also the signal generated using this method can be easily observed by the naked eye. Furthermore, the proposed strategy can well differentiate breast cancer patients from healthy individuals, demonstrating potential application in the analysis of clinical specimens.

Topics: Aptamers, Nucleotide; Benzothiazoles; Biosensing Techniques; Breast Neoplasms; Colorimetry; DNA, Catalytic; Exosomes; G-Quadruplexes; Hemin; Horseradish Peroxidase; Humans; Hydrogen Peroxide; Inverted Repeat Sequences; Limit of Detection; MCF-7 Cells; Mucin-1; Sulfonic Acids

The anticancer activity of acridone derivatives has attracted increasing interest, therefore, a variety of substituted analogs belonging to this family have been developed and evaluated for their anti-cancer properties. A series of

Topics: Acridines; Acridones; Benzothiazoles; Biphenyl Compounds; Breast Neoplasms; Cell Survival; DNA; DNA Topoisomerases, Type I; Female; Free Radical Scavengers; Humans; MCF-7 Cells; Picrates; Sulfonic Acids; Wound Healing

In the present study, we aimed to synthesize and investigate the impact of zinc oxide nanoparticle (ZnONPs) on both human and murine breast cancer cell lines and define their untoxic concentrations (IC. The in vitro study was initiated by ZnONPs green synthesizing process applying the Cucumis melo inodorus rough shell extract, and verified by the transmission electron microscope, scanning electron microscopy, Fourier-transform infrared spectroscopy, and X-ray diffraction analysis. In following, the human (Michigan Cancer Foundation-7 [MCF7]) and murine (TUBO) breast cancer cell lines were cultured for taking the time and dose-dependent treatment planes by ZnONPs. Also, MCF7 cell cultures were treated by three different doses of ZnoNPs (8, 4, and 2 µg/mL) separately and prepared for genes expression (Cas-3 and Cas-8) analysis using real-time quantitative PCR method. The in vivo initiated by providing the 39 murine breast cancer models, then they were injected intraperitoneally with different doses of ZnONPs (75, 50, and 25 mg/kg) treatments. Then their collected biopsies were stained by hematoxylin and eosin to evaluate their breast cancer tissue morphology and compare with Tamoxifen anticancer properties.. The in vitro study results demonstrate a significant correlation among the expression of Cas-3 and Cas-8 genes with increasing ZnONPs concentrations. The results of 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide assays for the treated cancer cell lines (MCF7 and TUBO) detected a significant negative correlation among the ZnONPs concentrations and the viability of the cells.. Unlike the majority of resent studies, we found the ZnONPs as a powerful apoptosis inducer in the human cell line (MCF7) and murine (TUBO cell line and cancer model).

Topics: Animals; Antineoplastic Agents; Apoptosis; Benzothiazoles; Breast Neoplasms; Caspase 3; Caspase 8; Cell Cycle; Cucumis melo; Disease Models, Animal; Female; Gene Expression Regulation, Enzymologic; Green Chemistry Technology; Humans; Inhibitory Concentration 50; Mammary Neoplasms, Animal; MCF-7 Cells; Metal Nanoparticles; Mice; Sulfonic Acids; Zinc Oxide

In our work, we evaluate the potential antioxidant, antimalarial activity and also activity against human breast cancer cells (MCF7) of Argan fruit extracts using in vitro models to validate the traditional use of this plant. Its chemical composition was also studied to begin the understanding of its activities, waiting to find the structure-activity relationship.. Polyphenols (89.4-218.5 eqGallic acid (mg/g dry)), tannins (39.3-214.0 eqCatechin (mg/g dry)), flavonoids (3.4-11.1 eqQuercetin (mg/g dry)) and anthocyanins (0.74-10.92 eqCyanindin (mug/g dry)) were quantified. A good (ethyl acetate and decoction) and moderate (petroleum ether) antioxidant activity were obtained for DPPH (IC(50) 32.3-600.8 microg/ml) and ABTS (IC(50) 11.9-988.8 microg/ml) assays. In addition, we found a good antimalarial activity (IC(50) 35 to >100 microg/ml) and human breast cancer cells activity (IC(50) 42 to >100 microg/ml).. The ethyl acetate extract and the decoction show interesting antimalarial and antioxidant activities. The results indicate a good correlations between anthocyanins quantitiy and the potential antioxidant (R(2)=0.9867) and also to antimalarial activity (R(2)=0.8175).

Topics: Antimalarials; Antineoplastic Agents, Phytogenic; Antioxidants; Benzothiazoles; Biphenyl Compounds; Breast Neoplasms; Cell Line, Tumor; Female; Flavonoids; Fruit; Humans; Inhibitory Concentration 50; Phenols; Phytotherapy; Picrates; Plant Extracts; Plasmodium falciparum; Sapotaceae; Sulfonic Acids; Tannins

Until now, very little has been known about the antioxidant capacity of squalene and its effect on human breast tumourigenesis. In the present work, we investigated squalene's scavenging properties and its effect on cell proliferation, cell cycle profile, apoptosis, reactive oxygen species (ROS) level and oxidative DNA damage, using human breast cell lines. Our results showed that squalene neither possesses scavenging activity nor significantly alters cell proliferation rates, the cell cycle profile or cell apoptosis in human mammary epithelial cells (MCF10A), minimally invasive (MDA-MB-231) breast cancer cells, and highly invasive (MCF7) breast cancer cells. However, we found that squalene did exert the following effects on MCF10A epithelial cells in a dose-dependent manner: (a) it decreased intracellular ROS level, (b) it prevented H(2)O(2)-induced oxidative injury, and (c) it protected against oxidative DNA damage. Interestingly, squalene did not exert these effects on MCF7 and MDA-MB-231 cancer cells. Therefore, our data suggest that squalene, found in high amounts in virgin olive oils, could be partially responsible for the lower incidence of breast cancer in populations that consume the Mediterranean diet due to its protective activity against oxidative DNA damage in normal mammary cells.

Topics: Apoptosis; Benzothiazoles; Breast; Breast Neoplasms; Cell Cycle; Cell Line, Transformed; Cell Line, Tumor; Cell Proliferation; DNA Damage; Dose-Response Relationship, Drug; Drug Antagonism; Epithelial Cells; Female; Free Radical Scavengers; Humans; Hydrogen Peroxide; Oxidative Stress; Squalene; Sulfonic Acids; Tumor Stem Cell Assay

Bioconversion of the isoflavonoid genistein to 2'- hydroxygenistein (2'-HG) was performed using isoflavone 2'-hydroxylase (CYP81E1) heterologously expressed in yeast. A monohydroxylated product was analyzed by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) and NMR spectrometry and was identified as 2'-HG. An initial bioconversion rate of 6% was increased up to 14% under optimized conditions. After recovery, the biological activity of 2'-HG was evaluated. Bioconverted 2'-HG showed higher antioxidant activity against 1,1- diphenyl-2-picryl hydrazine (DPPH) and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radicals than did genistein. Furthermore, 2'-HG exhibited greater antiproliferative effects in MCF-7 human breast cancer cells than did genistein. These results suggest that 2'- hydroxylation of genistein enhanced its antioxidant activity and cell cytotoxicity in MCF-7 human breast cancer cells.

Topics: Anticarcinogenic Agents; Antioxidants; Benzothiazoles; Biphenyl Compounds; Breast Neoplasms; Cell Line, Tumor; Cell Proliferation; Cell Survival; Chromatography, Liquid; Cytochrome P-450 Enzyme System; Dose-Response Relationship, Drug; Female; Genistein; Humans; Hydroxylation; Magnetic Resonance Spectroscopy; Picrates; Spectrometry, Mass, Electrospray Ionization; Sulfonic Acids