2--deoxyguanosine-5--phosphate and Colonic-Neoplasms

2--deoxyguanosine-5--phosphate has been researched along with Colonic-Neoplasms* in 1 studies

Other Studies

1 other study(ies) available for 2--deoxyguanosine-5--phosphate and Colonic-Neoplasms

Article	Year
Detection in situ of gamma-ray-induced DNA strand breaks in single cells: enzymatic labelling of free 3'-OH ends. International journal of radiation biology and related studies in physics, chemistry, and medicine, 1984, Volume: 46, Issue:5 We report a procedure allowing the detection and counting of free 3'-OH DNA strand extremities in single cells in situ. Terminal transferase (TdT) catalysed the incorporation of 3H-dGMP into fixed nuclei of human colonic adenocarcinoma cells (HT29), using free 3'-OH ends as initiator. Radioactivity was detected by autoradiography and determined quantitatively with a rapid image-processing system for grain counting. The initiator activity for TdT increases with the dose of gamma-rays in the dose range 2.5-20 Gy. Topics: Adenocarcinoma; Autoradiography; Cell Line; Cesium Radioisotopes; Colonic Neoplasms; Deoxyguanine Nucleotides; DNA Nucleotidylexotransferase; DNA, Neoplasm; Dose-Response Relationship, Radiation; Gamma Rays; Humans	1984

Article

Year

Detection in situ of gamma-ray-induced DNA strand breaks in single cells: enzymatic labelling of free 3'-OH ends.

International journal of radiation biology and related studies in physics, chemistry, and medicine, 1984, Volume: 46, Issue:5

We report a procedure allowing the detection and counting of free 3'-OH DNA strand extremities in single cells in situ. Terminal transferase (TdT) catalysed the incorporation of 3H-dGMP into fixed nuclei of human colonic adenocarcinoma cells (HT29), using free 3'-OH ends as initiator. Radioactivity was detected by autoradiography and determined quantitatively with a rapid image-processing system for grain counting. The initiator activity for TdT increases with the dose of gamma-rays in the dose range 2.5-20 Gy.

Topics: Adenocarcinoma; Autoradiography; Cell Line; Cesium Radioisotopes; Colonic Neoplasms; Deoxyguanine Nucleotides; DNA Nucleotidylexotransferase; DNA, Neoplasm; Dose-Response Relationship, Radiation; Gamma Rays; Humans

1984