2-((aminocarbonyl)amino)-5-(4-fluorophenyl)-3-thiophenecarboxamide and Lymphoma

2-((aminocarbonyl)amino)-5-(4-fluorophenyl)-3-thiophenecarboxamide has been researched along with Lymphoma* in 1 studies

Other Studies

1 other study(ies) available for 2-((aminocarbonyl)amino)-5-(4-fluorophenyl)-3-thiophenecarboxamide and Lymphoma

ArticleYear
Eomesodermin promotes interaction of RelA and NFATc2 with the Ifng promoter and multiple conserved noncoding sequences across the Ifng locus in mouse lymphoma BW5147 cells.
    Immunology letters, 2020, Volume: 225

    The T-box transcription factor Eomesodermin (Eomes) regulates the lineage-dependent expression of interferon γ (IFN-γ). We previously showed that Eomes promotes IFN-γ production and interacts with multiple conserved noncoding sequences (CNS) across the Ifng locus in mouse lymphoma BW5147 cells. In the present study, we investigated the transcriptional regulation of IFN-γ by the nuclear factor κB (NF-κB) subunit RelA and nuclear factor of activated T cells c2 (NFATc2, also known as NFAT1) in Eomes-transfected BW5147 cells. Eomes promoted the interaction of RelA and NFATc2 with the Ifng promoter and five CNS, including CNS-22 and CNS+30 upon stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin (IM). The dual NF-κB and STAT3 inhibitor TPCA-1 moderately reduced the PMA- and IM-induced IFN-γ transcription in Eomes-transfected BW5147 cells. TPCA-1 interfered with RelA binding to the Ifng promoter, CNS-22 and CNS+30. Moreover, TPCA-1 reduced the interaction of Eomes or NFATc2 with the Ifng promoter and CNS+30. The present results indicate that Eomes promotes the interaction of RelA and NFATc2 with the Ifng promoter and multiple CNS across the Ifng locus in BW5147 cells.

    Topics: Amides; Animals; Cell Line, Tumor; Conserved Sequence; Gene Expression Regulation, Neoplastic; Genetic Loci; Interferon-gamma; Lymphoma; Mice; NFATC Transcription Factors; Promoter Regions, Genetic; Protein Kinase Inhibitors; STAT3 Transcription Factor; T-Box Domain Proteins; Thiophenes; Transcription Factor RelA

2020