17-ketosteroids and Precancerous-Conditions

Topics: 17-Ketosteroids; Adolescent; Adrenal Gland Neoplasms; Adrenocorticotropic Hormone; Adult; Cushing Syndrome; Female; Humans; Hyperplasia; Male; Middle Aged; Pituitary Neoplasms; Precancerous Conditions

Previous work has demonstrated that dehydroepiandrosterone (DHEA) strongly inhibits growth and de novo cholesterol (CH) biosynthesis in preneoplastic rat liver. Administration of a mixture of 4 ribo- or deoxyribonucleosides of adenine, guanine, cytosine and uracil/thymine, prevents growth inhibition but not inhibition of CH synthesis. The purpose of this paper was to identify the site of inhibition of CH synthesis by DHEA. Persistent nodules (PNs) were induced, in diethylnitrosamine-initiated male F344 rats, by 'resistant hepatocyte' protocol. Fifteen weeks after initiation, nodule bearing rats and normal controls received a diet containing 0.6% DHEA for 3 weeks. They were then killed. 3-Hydroxy-3-methylglutaryl-CoA reductase (HMGR) activity and mRNA levels were 18- and 14-fold higher, respectively in nodules than in normal liver. DHEA strongly inhibited HMGR activity in both tissues in vivo, but had a slight effect on HMGR activity, when added in vitro to the reaction mixture for determination of this activity. In vivo DHEA treatment caused a 65% decrease in the level of HMGR mRNA in PNs, which, however, does not seem to completely account for the decrease in HMGR activity (83%). Low density lipoprotein receptor (LDL-R) mRNA level underwent a slight decrease in PNs, with respect to control liver, which did not lead to a significant decrease in 125I-LDL binding to LDL-R. DHEA treatment caused 30% and 24% increases in LDL-R expression and 125I-LDL binding, respectively, in nodules. These observations indicate that in addition to HMGR gene expression, increased influx of LDL into preneoplastic cells may contribute to the deregulation of mevalonate synthesis by DHEA. The observation that HMGR activity and gene expression were still 3- to 5-fold higher in PNs of DHEA-treated rats than in control liver, and previous findings of preneoplastic liver cell growth in the presence of relatively low CH synthesis, suggest that even relatively low levels of mevalonate are sufficient for the growth of preneoplastic liver cells.

Topics: 17-Ketosteroids; Animals; Body Weight; Cholesterol; Dehydroepiandrosterone; Gene Expression; Hydroxymethylglutaryl CoA Reductases; Hydroxymethylglutaryl-CoA Reductase Inhibitors; Lipoproteins, LDL; Liver; Liver Neoplasms, Experimental; Male; Mevalonic Acid; Precancerous Conditions; Rats; Rats, Inbred F344; Receptors, LDL

The glucuronic acid level in the serum and urine, the diural excretion of estrogens and 17-KS with the urine, the activity of glucuronyltransferase are reported in 101 patients with benign and malignant tumors and in 22 healthy females. The decrease in the activity of glucurolytransferase and increase in the percentage of free (nonconjugated) estrogens in patients with malignant tumors of the mammary gland are noted, the analogous results were obtained in patients with benign tumors, but these are statistically insignificant. The decrease of the glucuronyltransferase activity is suggested to result in the development of relative hyperestrogen in normal total excretion.

Topics: 17-Ketosteroids; Adult; Breast Neoplasms; Enzyme Activation; Estrogens; Estrogens, Conjugated (USP); Female; Free Radicals; Glucuronates; Glucuronosyltransferase; Humans; Middle Aged; Precancerous Conditions

Case-report about a 56 year old white female patient with pachydermia laryngis and hirsutism, successfully treated with cyproterone-acetate, a testosterone antagonist. The significance of androgenic hormones for the development of epithelial changes in the female larynx is emphasized. The administration of antiandrogenic substances as a new therapeutic measure is discussed.

Topics: 17-Hydroxycorticosteroids; 17-Ketosteroids; Cyproterone; Estrogens; Female; Hirsutism; Humans; Laryngeal Neoplasms; Middle Aged; Precancerous Conditions; Testosterone; Vocal Cords

Topics: 17-Ketosteroids; Adrenal Cortex; Adult; Aged; Alanine Transaminase; Aspartate Aminotransferases; Blood Proteins; Estrogens; Female; Humans; Liver; Middle Aged; Ovary; Precancerous Conditions; Vulvar Neoplasms

Measurement of the excretion of total 17-ketosteroids, androsterone, etiocholanolone, and dehydroepiandrosterone demonstrate in contrast to the well known age dependency of normal women in cases of both mastopathia and breast cancer the following results: 1. the age dependence of the 17-ketosteriod excretion is not marked; 2. the total 17-ketosteroid excretion is lower than that of normal controls, 3. the values of the etiocholanolone excretions and that of the dehydroepiandrosterone excretions are similarly lower.

Topics: 17-Ketosteroids; Adult; Androsterone; Breast Neoplasms; Dehydroepiandrosterone; Etiocholanolone; Female; Humans; Menopause; Middle Aged; Precancerous Conditions

Comparative investigations of the urinary excretion of different 17-ketosteroids in healthy women and patients suffering from mastopathia fibrocystica and/or mammary carcinomas had the following results: 1. A clear age dependency of the excretion of 17 ketosteroids during the regenerative period has been shown in all groups investigated, but it could not be observed during the postmenopausal phase. 2. There are no hints by the excretion values for a remarkable contribution of mammary carcinomas to the hormone metabolism of the host. 3. The excretion values of patients suffering from mastopathia fibrocystica confirm epidemiological results, which consider this from of mastopathia a precancerous one.

Topics: 17-Ketosteroids; Adult; Age Factors; Aged; Androsterone; Breast Diseases; Breast Neoplasms; Cysts; Dehydroepiandrosterone; Etiocholanolone; Female; Humans; Middle Aged; Precancerous Conditions

Topics: 17-Hydroxycorticosteroids; 17-Ketosteroids; Adult; Aged; Androsterone; Chromatography, Thin Layer; Cortisone; Dehydroepiandrosterone; Etiocholanolone; Female; Glucocorticoids; Humans; Hydrocortisone; Middle Aged; Precancerous Conditions; Tetrahydrocortisol; Tetrahydrocortisone; Uterine Neoplasms

Topics: 17-Hydroxycorticosteroids; 17-Ketosteroids; Adolescent; Adrenal Gland Diseases; Adrenal Gland Neoplasms; Adrenalectomy; Adult; Carcinoma; Cushing Syndrome; Diagnosis, Differential; Humans; Hyperplasia; Precancerous Conditions

Topics: 17-Ketosteroids; Adult; Dehydroepiandrosterone; Female; Humans; Middle Aged; Precancerous Conditions; Uterine Cervical Neoplasms

Topics: 17-Hydroxycorticosteroids; 17-Ketosteroids; Adolescent; Adrenal Glands; Adrenocorticotropic Hormone; Adult; Female; Gastritis; Humans; Male; Middle Aged; Pituitary-Adrenal Function Tests; Pituitary-Adrenal System; Polyps; Precancerous Conditions; Stomach Neoplasms

Topics: 17-Ketosteroids; Adult; Aged; Breast Neoplasms; Gynecomastia; Humans; Male; Middle Aged; Precancerous Conditions; Sex Chromatin