15-keto-13-14-dihydroprostaglandin-f2alpha has been researched along with Swine-Diseases* in 7 studies
7 other study(ies) available for 15-keto-13-14-dihydroprostaglandin-f2alpha and Swine-Diseases
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Changes in prostaglandin production and ovarian function in gilts during endometritis induced by Escherichia coli infection.
The aim of this study was to determine the prostaglandins (PGs) production and ovarian function in gilts after intrauterine infusions of 10(6) and 10(9) colony-forming units (cfu)/ml of Escherichia coli (E. coli). In Experiments 1 and 2, 30 ml of saline or 30 ml of E. coli suspension containing 10(6) or 10(9)cfu/ml, were infused once into each uterine horn in three groups of gilts on day 3 of the estrous cycle, respectively. In Experiment 1, 17 days after treatment it was revealed that inoculation of E. coli 10(9)cfu/ml induced severe acute or subacute endometritis while 10(6)cfu of E. coli evoked moderate acute endometritis or resulted in no inflammatory changes. In the gilts receiving 10(9)cfu/ml of E. coli, the concentration of 13,14-dihydro-15-keto-PGF(2)alpha in blood from the jugular vein was elevated (P<0.05-0.001) compared to concentration in the gilts inoculated with 10(6)cfu on days 8-17 after treatment. Both the E. coli-treated groups had a lower (P<0.05, P<0.01) progesterone plasma level from days 10 to 14 after administration than the control group. On day 17 of the study, infusion of E. coli 10(9)cfu/ml, in comparison to 10(6)cfu, resulted in the greater (P<0.001) content of PGE(2) in the myometrium. The content of both PGs in the endometrium as well as PGF(2alpha) in the myometrium of gilts-treated with 10(9)cfu/ml of E. coli was lower (P<0.001) than in gilts-treated with 10(6)cfu of bacteria. Newly formed corpora lutea were found in the gilts infused with 10(6), but not those infused with 10(9)cfu/ml of E. coli on day 17 after infusion. On day 8 of the study (Experiment 2), the blood from utero-ovarian vein of the gilts-treated with 10(9)cfu/ml of bacteria had a higher (P<0.05) PGF(2alpha) level and lower (P<0.001) PGE(2) level than following infusion of E. coli 10(6)cfu/ml. Also on day 8 of the study, the content of PGE(2) in the endometrium, both the PGs in the myometrium as well as cyclooxygenase-2 in the endometrium and myometrium was greater (P<0.01, P<0.001) after applying 10(9)cfu/ml than 10(6)cfu/ml of E. coli. These results indicate that intrauterine infusions of 10(6) or 10(9)cfu/ml of E. coli lead to the development of inflammatory states of different intensities which is connected with different PGF(2alpha) and PGE(2) production and function of ovaries. Topics: Animals; Colony Count, Microbial; Dinoprost; Endometritis; Escherichia coli Infections; Female; Ovary; Random Allocation; Swine; Swine Diseases | 2007 |
The impact of induced stress during days 13 and 14 of pregnancy on the composition of allantoic fluid and conceptus development in sows.
Stress due to regrouping of breeding females is difficult to avoid completely in loose-housing systems. The effects of stress during the maternal recognition of pregnancy on fetal development and survival at Day 30 of pregnancy was, therefore, studied in 17 sows allocated into one control (C-) group, one group deprived of food during Days 13 and 14 (FD-), and one group (A-), which was treated with ACTH (0.01 mg/kg body weight of Synacthen Depot) every sixth hour during the same period. Total number of fetuses, fetal survival rate, volume of allantoic fluid, and the weight and length of total fetal unit, placentas, allantochorion and fetuses were determined. The concentrations of progesterone (P4), PGFM, PGF2, PGE, estrone-sulfate, and estradiol-17beta in the allantoic fluid were analyzed. No significant differences between groups were found for any parameter measured except for P4. Food deprivation increased P4 concentration in the allantoic fluid, and there was a positive correlation between the P4 concentration and the weight of the placenta. It is, therefore, suggested that P4 influences the placenta size among food-deprived sows. Topics: Allantois; Animals; Body Fluids; Dinoprost; Embryonic and Fetal Development; Estradiol; Estrone; Female; Fetal Weight; Gestational Age; Organ Size; Placenta; Pregnancy; Pregnancy Complications; Progesterone; Prostaglandins E; Stress, Physiological; Swine; Swine Diseases | 2004 |
Plasma levels of cortisol, progesterone, oestradiol-17 beta and prostaglandin F2 alpha metabolite after ACTH (Synacthen Depot) administration in ovariectomized gilts.
In order to elucidate the effect of stress on reproductive hormones, the present study was designed to investigate the effect of adrenocorticotropic hormone (ACTH) on the plasma levels of cortisol, progesterone, oestradiol-17 beta and prostaglandin F2 alpha metabolite in ovariectomized gilts. Ovariectomy and cannulation of the jugular vein were performed within 1 week of oestrous detection, under general anaesthesia. Approximately 1 week after surgery, two gilts were each administered ACTH (Synacthen Depot) intravenously, at a dose of 0.01 mg/kg body weight, and one gilt was given saline solution (5 ml). The reverse was performed on the following day. The administration of ACTH was followed by a concomitant elevation of cortisol, progesterone and prostaglandin F2 alpha metabolite but not of oestradiol-17 beta. Peak cortisol, progesterone and prostaglandin F2 alpha metabolite levels were reached at 80 +/- 10.0, 80 +/- 10.0 and 46.6 +/- 13.3 min after ACTH administration and the durations of the peaks were 181.8 +/- 19.8, 308.1 +/- 49.7 and 181.8 +/- 7.9 min, respectively. The total area under the curve for cortisol, progesterone and prostaglandin F2 alpha metabolite was significantly higher in the ACTH than in the control group. The present results indicate that during stress, cortisol, progesterone and prostaglandin F2 alpha levels are elevated while the level of oestradiol-17 beta is less affected. It can be concluded that the administration of ACTH to ovariectomized gilts, results in the elevation of cortisol, progesterone and prostaglandin F2 alpha metabolite but not of oestradiol-17 beta. Topics: Adrenocorticotropic Hormone; Animals; Area Under Curve; Delayed-Action Preparations; Dinoprost; Estradiol; Estrus Detection; Female; Hydrocortisone; Male; Ovariectomy; Progesterone; Radioimmunoassay; Stress, Physiological; Swine; Swine Diseases | 2000 |
A clinical and endocrine study on the effect of orally administered bacterial endotoxin in adult pigs and goats.
Endotoxin of Gram-negative bacteria was orally administered in 5 female pigs and 8 male goats. Two of the gilts were pregnant. A solution of lipopolysaccharide (LPS) of Enterobacter agglomerans was mixed into the feed ration of the pigs (40 mg/animal), and given by gastric tube into the rumen of the goats (1-20 mg/animal). Jugular venous blood was collected and clinical signs, rectal temperature and WBC counts were recorded for 12-24 hours. Plasma concentrations of the major plasma metabolite of PGF2 alpha, 15-keto-13,14-dihydro-PGF2 alpha were determined in both species, progesterone concentration only in pigs. The pigs showed slight to severe signs of endotoxemia. Increases in rectal temperature and levels of the PGF2 alpha metabolite occurred in 3 gilts. Progesterone level and the total WBC counts remained unchanged. Differential counts followed irregular patterns mostly within the normal range. The goats showed slight signs of discomfort. Temperature increased in one animal. No other parameters were altered after the intake of LPS. The observations in pigs indicate that endotoxin either penetrated the intestinal barrier causing systemic endotoxemia or induced inflammatory reactions in the intestine activating inflammatory mediators. Topics: Administration, Oral; Animals; Dinoprost; Endotoxins; Enterobacter; Enterobacteriaceae; Female; Goat Diseases; Goats; Leukocyte Count; Male; Pregnancy; Pregnancy Complications, Infectious; Progesterone; Swine; Swine Diseases; Toxemia | 1990 |
Clinical, endocrinological and spermatological studies after endotoxin injection in the boar.
Three adult boars were injected intravenously with endotoxin from Salmonella typhimurium. Blood plasma was analysed for the contents of 15-ketodihydro-PGF2 alpha, LH and testosterone. Total amount of white blood cells and differential counts were determined in whole blood. Semen was examined for concentration, motility, volume and morphological appearance of the spermatozoa. The boars were slaughtered three months after the endotoxin injection and the testes were examined. The total number of white blood cells decreased and the levels of 15-ketodihydro-PGF2 alpha rose immediately after the endotoxin administration. An initial increase in LH was seen in two out of the three boars. The increase in LH was followed by a testosterone increase in one boar and a testosterone decrease in the other. The third boar showed no initial increase in LH but an increase in testosterone. Semen examination demonstrated various morphological changes of the spermatozoa in all boars. The changes started to appear at about the same time after the endotoxin injection, but differed among the individuals. The examination of the testes depicted no changes from what is seen in normal animals. The present results indicate that the boar responds to endotoxin similar to what is seen in the ram. The endocrine changes, e.g. in LH and testosterone, are similar to those seen after termination of heat stress. The seminal changes indicate a disturbance located in the epididymis as well as a short-term, mild degeneration in the seminiferous epithelium. Topics: Animals; Dinoprost; Endotoxins; Luteinizing Hormone; Male; Salmonella typhimurium; Sperm Count; Sperm Motility; Swine; Swine Diseases; Testosterone | 1989 |
Hormonal changes in sows after induced porcine parvovirus infection in early pregnancy.
Hormonal changes, lesions, and virus isolation studies were determined in sows after uterine artery inoculation with porcine parvovirus [( PPV], strain NADL-8) in early pregnancy. Two sows were given PPV on days 14 or 16 and were euthanatized and necropsied on day 35 after twice daily plasma collection for hormone measurement. Parvovirus was given to 4 sows on day 14 and to 4 sows on day 21 with 5 times daily plasma samples collected for 1 week. Sows were examined on days 21 and 28, respectively. Four control sows in each group on days 14 and 21 were given a placebo injection and were similarly studied. All embryos in all but 1 sow given PPV were in various stages of resorption at necropsy. Normal embryos were present in all control sows. Estrone sulfate values increased logarithmically, progesterone values remained stable, and concentrations of 13, 14-dihydro-15-keto-prostaglandin (PG) F2 alpha (PGFM), a PGF2 alpha metabolite, were less than 200 ng/ml for sows given a placebo. In contrast, sows with resorbing embryos did not have an increase in estrone sulfate values. A decrease in plasma progesterone values occurred in 9 of 10 sows inoculated with PPV; this decrease was accompanied by greater than or equal to 1 marked increase in PGFM concentrations. Quantitative assessment of the uterus revealed significantly greater cytoplasmic density in endometrial and glandular cell (P less than 0.01), a greater glandular epithelium height (P less than 0.05), and twice the number of glands (P less than 0.05) in control sows, compared with values in sows inoculated with PPV.(ABSTRACT TRUNCATED AT 250 WORDS) Topics: Animals; Dinoprost; Embryo Loss; Estrogens; Female; Gonadal Steroid Hormones; Hemagglutination Tests; Parvoviridae; Parvoviridae Infections; Pregnancy; Pregnancy Complications, Infectious; Progesterone; Prostaglandins F; Swine; Swine Diseases; Uterus | 1987 |
Clinical and endocrinological studies in primiparous zero-weaned sows. 3. Hormonal patterns of ovarian disorders due to zero-weaning.
Topics: Animals; Dinoprost; Estradiol; Female; Hormones; Lactation; Luteinizing Hormone; Ovarian Cysts; Pregnancy; Progesterone; Prostaglandins F; Puerperal Disorders; Swine; Swine Diseases | 1983 |