15-keto-13-14-dihydroprostaglandin-f2alpha and Puerperal-Disorders

The objective of this study was to investigate the effects of puerperal uterine disease on uterine blood flow using trans-rectal Doppler sonography. Lactating Holstein Friesian cows (n=44) were divided into two groups based on whether they were healthy (UD-; n=23) or had uterine disease (UD+; n=21) defined as retained fetal membranes and/or metritis. General clinical examination, vaginoscopy, trans-rectal palpation, and trans-rectal B-Mode sonography were conducted on Days 8, 11, 18, 25 and then every 10 days until Day 65 after calving. Doppler sonography of the uterine arteries was conducted on Day 8, during diestrus after the second ovulation (Days 40-60 after calving) and during diestrus before breeding (Days 63-75 after calving). Cows with uterine disease had greater (P<0.05) uterine size as assessed trans-rectally compared with cows of the UD group. Sonographic measurements on Day 11 after parturition revealed a greater (P<0.05) horn diameter in cows of the UD+ than in the UD- group. Both uterine size and uterine horn diameter decreased more earlier following parturition (P<0.05) in cows of the UD- group. Blood flow volume (BFV) was greater and pulsatility index was less on Day 8 after calving in cows of UD+ than UD- group (P<0.05). In cows of the UD-, but not in those of the UD+ group, there was a further reduction in BFV subsequent to Day 45 after calving (P<0.05). The results of this study show that uterine blood flow measures by trans-rectal Doppler sonography are affected by puerperal uterine disease.

Topics: Animals; Cattle; Cattle Diseases; Dairying; Dinoprost; Female; Puerperal Disorders; Regional Blood Flow; Ultrasonography, Doppler; Uterine Artery; Uterine Diseases; Uterus

The objective of the present study was to validate a simple, sensitive and direct enzymeimmunoassay (EIA) procedure for 13,14-dihydro-15-keto-PGF(2alpha) (PGFM) for use in buffaloes with postpartum reproductive disorders and determine the practicalities of using plasma concentrations of 13,14-dihydro-15-keto-PGF(2alpha) for monitoring their reproductive health. The EIA was used for determination of the circulating levels of PGFM associated with the retention of fetal membranes, postpartum endometritis and variable postpartum intervals. The concentrations of PGFM with retention of fetal membranes in the periparturient period were lower as compared to buffaloes that had uneventful parturitions. Concentrations of PGFM associated with postpartum endometritis were elevated as compared to those in buffaloes free of reproductive tract infections. Buffaloes having higher plasma concentrations of PGFM in early postpartum period had shorter postpartum intervals, indicating the association between PGFM concentrations postpartum and uterine involution as well as the resumption of estrous cycle in this species. The study presents the possibility of using circulating PGFM concentrations for monitoring the postpartum reproductive health of buffaloes.

Topics: Animals; Antibody Specificity; Buffaloes; Dinoprost; Endometritis; Female; Immunoenzyme Techniques; Placenta, Retained; Postpartum Period; Pregnancy; Puerperal Disorders; Reproducibility of Results; Reproduction; Sensitivity and Specificity; Uterine Diseases

The aim of this work was to investigate possible differences in peripheral blood concentrations of 13, 14 dihydro 15 keto PGF alpha (PGFM) and 6 keto PGF1 alpha (PGIM), between dairy cows retaining (RP) and not retaining (NRP) their placenta. Blood samples were collected 5, 30, and 60 minutes postpartum from 8 RP and 17 NRP cows. PGFM concentrations were significantly lower in RP cows than in NRP cows 60 minutes after birth (8156 pg/ml vs 12016 pg/ml; P less than or equal to 0.05). There was a significant rise of PGFM levels between 30 and 60 minutes in NRP cows (6225 pg/ml vs 12016 pg/ml; P less than or equal to 0.01), while in RP cows PGFM levels remained fairly constant along the period studied. No significant differences were found between RP and NRP cows regarding PGIM levels during this period although absolute values were slightly higher in RP cows. The PGFM/PGIM ratio was significantly higher 60 minutes postpartum in NRP cows than in RP cows (15.5 vs 8.8; P less than or equal to 0.01). This ratio increased significantly in NRP cows between 30 and 60 minutes (7.2 vs 15.5; P less than or equal to 0.01), a pattern not observed in RP cows. There was no correlation between PGIM and PGFM levels during the first 60 minutes postpartum in the NRP cows. However, the same correlation was significantly positive in the RP cows (r = 0.75; P less than or equal to 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)

Topics: 6-Ketoprostaglandin F1 alpha; Animals; Cattle; Cattle Diseases; Dinoprost; Epoprostenol; Female; Placenta; Pregnancy; Prostaglandins F; Puerperal Disorders; Radioimmunoassay; Time Factors

Topics: Animals; Dinoprost; Estradiol; Female; Hormones; Lactation; Luteinizing Hormone; Ovarian Cysts; Pregnancy; Progesterone; Prostaglandins F; Puerperal Disorders; Swine; Swine Diseases