15-hydroxy-5-8-11-13-eicosatetraenoic-acid and Wounds--Penetrating

Uncovered fetal rabbit excisional wounds do not exhibit any classic signs of healing; wounds covered with an impermeable cover do contract, reepithelialize, and exhibit inflammation. Prostaglandin E2 (PGE2) is elevated in amniotic fluid, acting as an immunosuppressant at the maternal-fetal interface. Full-thickness excisional wounds were made on 25-day gestational age rabbit fetuses. Half the wounds were covered with an impermeable cover. Tissue from covered, uncovered, and nonwounded fetuses was examined 72 h after wounding for arachidonic acid metabolites. Uncovered wounds had significantly (P less than or equal to 0.05) elevated levels of PGE2, PGF2 alpha, and 12-HETE versus covered wounds and control tissue. Covered wounds had significantly elevated levels of 15-HETE compared to uncovered and control tissue. The elevated PGE2 in uncovered wounds may act as a fetal immunosuppressant; covered wounds (lower PGE2) developed cellular inflammation. Further investigations of these interactions may permit modulation of adult inflammation.

Topics: 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; Amniotic Fluid; Animals; Arachidonic Acids; Dinoprost; Dinoprostone; Female; Fetus; Gestational Age; Hydroxyeicosatetraenoic Acids; Inflammation; Leukotrienes; Occlusive Dressings; Pregnancy; Prenatal Injuries; Rabbits; Silicones; Sutures; Wound Healing; Wounds, Penetrating