Compounds > 15-hydroxy-5-8-11-13-eicosatetraenoic-acid

15-hydroxy-5-8-11-13-eicosatetraenoic-acid and Eosinophilia

15-hydroxy-5-8-11-13-eicosatetraenoic-acid has been researched along with Eosinophilia* in 1 studies

Other Studies

1 other study(ies) available for 15-hydroxy-5-8-11-13-eicosatetraenoic-acid and Eosinophilia

Article	Year
Comparison of 5- and 15-lipoxygenase activities in blood and alveolar leukocyte preparations from normal subjects and patients with eosinophilia. Prostaglandins, leukotrienes, and medicine, 1986, Volume: 23, Issue:2-3 The arachidonic acid lipoxygenase metabolites of polymorphonuclear leukocyte (PMNL) preparations obtained from 31 subjects with eosinophilia (eosinophil content of PMNL preparations: 27.0%, 4-73, median with range) and 29 normal donors (4.5%, 0-15) were analyzed by reversed phase high performance liquid chromatography. More leukotriene (LT) C4 and 15-hydroxyeicosatetraenoic acid (HETE) (p less than 0.001) and less LTB4 (p less than 0.005) were found in eosinophil-rich preparations in comparison to controls on incubations with ionophore and arachidonic acid. In incubations with arachidonic acid alone, only small amounts of 5-lipoxygenase metabolites were produced by both groups, but the PMNL preparations from patients with eosinophilia showed higher capacity to release LTC4 and 15-HETE (p less than 0.005). In the eosinophil-rich preparations, the percentage of eosinophils correlated positively with the production of LTC4 and negatively with LTB4 (p less than 0.05, incubations with ionophore +/- arachidonic acid), and positively with 15-HETE (p less than 0.01, incubations with arachidonic acid +/- ionophore). Moreover the eosinophil-rich preparations produced more LTC4 per eosinophil in incubations with arachidonic acid alone (p less than 0.001) than normal PMNL preparations. The predominant release of LTC4 and 15-HETE by eosinophils was further confirmed by the analysis of bronchoalveolar lavage cells. Lavage cells containing eosinophils released LTC4 and 15-HETE while normal bronchoalveolar lavages, which were poor in eosinophils, did not produce detectable amount of LTC4 and 15-HETE. These results show that eosinophils release preferentially LTC4 and 15-HETE in contrast to LTB4 for the neutrophils. Topics: Adolescent; Adult; Aged; Arachidonate 15-Lipoxygenase; Arachidonate 5-Lipoxygenase; Arachidonate Lipoxygenases; Arachidonic Acids; Child; Chromatography, High Pressure Liquid; Eosinophilia; Eosinophils; Female; Humans; Hydroxyeicosatetraenoic Acids; Leukocytes; Leukotriene B4; Macrophages; Male; Middle Aged; Neutrophils; Pulmonary Alveoli; Reference Values; SRS-A	1986

Article

Year

Comparison of 5- and 15-lipoxygenase activities in blood and alveolar leukocyte preparations from normal subjects and patients with eosinophilia.

Prostaglandins, leukotrienes, and medicine, 1986, Volume: 23, Issue:2-3

The arachidonic acid lipoxygenase metabolites of polymorphonuclear leukocyte (PMNL) preparations obtained from 31 subjects with eosinophilia (eosinophil content of PMNL preparations: 27.0%, 4-73, median with range) and 29 normal donors (4.5%, 0-15) were analyzed by reversed phase high performance liquid chromatography. More leukotriene (LT) C4 and 15-hydroxyeicosatetraenoic acid (HETE) (p less than 0.001) and less LTB4 (p less than 0.005) were found in eosinophil-rich preparations in comparison to controls on incubations with ionophore and arachidonic acid. In incubations with arachidonic acid alone, only small amounts of 5-lipoxygenase metabolites were produced by both groups, but the PMNL preparations from patients with eosinophilia showed higher capacity to release LTC4 and 15-HETE (p less than 0.005). In the eosinophil-rich preparations, the percentage of eosinophils correlated positively with the production of LTC4 and negatively with LTB4 (p less than 0.05, incubations with ionophore +/- arachidonic acid), and positively with 15-HETE (p less than 0.01, incubations with arachidonic acid +/- ionophore). Moreover the eosinophil-rich preparations produced more LTC4 per eosinophil in incubations with arachidonic acid alone (p less than 0.001) than normal PMNL preparations. The predominant release of LTC4 and 15-HETE by eosinophils was further confirmed by the analysis of bronchoalveolar lavage cells. Lavage cells containing eosinophils released LTC4 and 15-HETE while normal bronchoalveolar lavages, which were poor in eosinophils, did not produce detectable amount of LTC4 and 15-HETE. These results show that eosinophils release preferentially LTC4 and 15-HETE in contrast to LTB4 for the neutrophils.

Topics: Adolescent; Adult; Aged; Arachidonate 15-Lipoxygenase; Arachidonate 5-Lipoxygenase; Arachidonate Lipoxygenases; Arachidonic Acids; Child; Chromatography, High Pressure Liquid; Eosinophilia; Eosinophils; Female; Humans; Hydroxyeicosatetraenoic Acids; Leukocytes; Leukotriene B4; Macrophages; Male; Middle Aged; Neutrophils; Pulmonary Alveoli; Reference Values; SRS-A

1986