12-hydroxy-5-8-10-14-eicosatetraenoic-acid and Dermatitis

Topics: 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; Arachidonic Acids; Dermatitis; Fatty Acids; Humans; Lipoxygenase; Prostaglandin-Endoperoxide Synthases; Prostaglandins

A model approach is presented to the in vivo inflammation cascade, in which the activities of key enzymes (phospholipase A2 [3.1.1.4], prostaglandin synthase [EC 1.14.99.1], and lipoxygenases [EC 1.13.11.12]) are determined simultaneously in a single in vitro assay.. Detection of phospholipids (up to 50 pM) and arachidonic acid (up to 33 pM) is attained with high sensitivity and without radiolabelling using a SEDERE light scattering detector.. Thus, in combination with a diode array UV-detector, lipids, prostaglandins, HETEs and other metabolites of the inflammation cascade can be determined with high efficiency using a reversed phase-high performance liquid chromatograph equipped with two highly sensitive detectors in series.

Topics: 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; Animals; Arachidonate 12-Lipoxygenase; Arachidonic Acid; Chromatography, High Pressure Liquid; Dermatitis; Lipoxygenase; Male; Phospholipases A; Phospholipases A2; Phospholipids; Prostaglandin H2; Prostaglandin-Endoperoxide Synthases; Prostaglandins H; Sheep; Swine

Topics: 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; Arachidonic Acid; Arachidonic Acids; Dermatitis; Drug Tolerance; Forearm; Humans; Hydroxyeicosatetraenoic Acids; Leukotriene B4; Lipoxygenase; Psoriasis; Skin; Skin Tests; SRS-A; Time Factors