11-mercaptoundecanoic-acid and Cystic-Fibrosis

11-mercaptoundecanoic-acid has been researched along with Cystic-Fibrosis* in 1 studies

Other Studies

1 other study(ies) available for 11-mercaptoundecanoic-acid and Cystic-Fibrosis

ArticleYear
Electrochemical analysis of cell plasma membrane cholesterol at the airway surface of mouse trachea.
    Analytical chemistry, 2008, Feb-15, Volume: 80, Issue:4

    Electrochemical detection of plasma membrane cholesterol at the surface of excised mouse trachea tissue is reported. Cholesterol oxidase is covalently linked to an 11-mercaptoundecanoic acid submonolayer on the platinum electrode surface. The cholesterol oxidase-modified electrodes show steady-state responses for cholesterol in solution at physiological temperatures. Experiments for direct contact between the cholesterol oxidase-modified electrode and the surface of excised trachea tissue at 37 degrees C indicate steady-state responses that are largely independent of the position of contact on the tissue surface. Tissue samples are mounted on a quartz crystal microbalance electrode to gauge contact force between the electrode and the tissue surface, and the steady-state electrode response for tissue cholesterol is shown to be largely independent of the contact force. Trachea tissue excised from a mouse model of cystic fibrosis, which is known to exhibit evaluated cholesterol in airway cells, shows an electrode response that is approximately 40% larger than the response observed at wild-type mouse trachea tissue.

    Topics: Animals; Biosensing Techniques; Cell Membrane; Cholesterol; Cholesterol Oxidase; Cystic Fibrosis; Disease Models, Animal; Electrochemistry; Fatty Acids; Mice; Microelectrodes; Platinum; Respiratory Mucosa; Sulfhydryl Compounds; Trachea

2008