10-nitro-oleic-acid and Spondylitis--Ankylosing

Inflammatory arthritis including rheumatoid arthritis (RA) and spondyloarthritis (SpA) is characterized by inflammation and destruction of the joints. Approximately one third of patients do not respond to first-line treatments. Nitro-fatty acids are bioactive lipids with anti-inflammatory properties and tissue-protective functions. The nitro-fatty acid 10-NO. Synovial fluid and blood samples were obtained from 14 patients with active RA or SpA. The in vitro models consisted of synovial fluid mononuclear cells (SFMCs) cultured for 48 h, SFMCs cultured for 21 days, and fibroblast-like synovial cells (FLSs) co-cultured with peripheral blood mononuclear cells (PBMCs) for 48 h. Cells were treated with or without 10-NO. 10-NO

Topics: Adult; Anti-Inflammatory Agents; Arthritis, Rheumatoid; Cells, Cultured; Chemokine CCL2; Coculture Techniques; Etanercept; Female; Fibroblasts; Humans; Interferon Type I; Leukocytes, Mononuclear; Male; Middle Aged; Oleic Acids; Spondylitis, Ankylosing; Synovial Fluid