10-hydroxystearic-acid and Colonic-Neoplasms

10-hydroxystearic-acid has been researched along with Colonic-Neoplasms* in 2 studies

Other Studies

2 other study(ies) available for 10-hydroxystearic-acid and Colonic-Neoplasms

Article	Year
Gas chromatography/mass spectrometric assay of endogenous cellular lipid peroxidation products: quantitative analysis of 9- and 10-hydroxystearic acids. Rapid communications in mass spectrometry : RCM, 2002, Volume: 16, Issue:9 A sensitive, specific, accurate and reproducible gas chromatography/mass spectrometry method was developed for the assay of 9- and 10-hydroxystearic acids in samples obtained as cell extracts. The preparation of the samples required specific procedures to allow the analysis of both the free and the conjugated hydroxy acids as the corresponding methyl esters. The quantification used propyl-paraben as the internal standard and monitoring of a specific fragment of each isomeric hydroxy acid methyl ester, and allowed quantification of the conjugate and the free fractions of both 9- and 10-hydroxystearic acids. This method is suitable for identification and quantification (LOQ 1.8 and 4.4 ng, respectively) of these important metabolites of lipid peroxidation. In particular the development of an assay for the free 9-hydroxystearic acid methyl ester makes the method a reliable analytical tool for investigations of the role of this metabolite in the mechanisms of tumour cell proliferation. Topics: Calibration; Carcinoma; Colonic Neoplasms; Gas Chromatography-Mass Spectrometry; Humans; Lipid Peroxidation; Reproducibility of Results; Sensitivity and Specificity; Stearic Acids; Tumor Cells, Cultured	2002
In vitro effects of hydroxystearic acid on the proliferation of HT29 and I407 cells. Biochemistry and molecular biology international, 1994, Volume: 33, Issue:4 The in vitro effects of hydroxystearic acid on the proliferation of human colon carcinoma cells (HT29) and human embryonic intestine cells (I407) were examined and compared to previous results obtained in murine C108 lung carcinoma cells. The cells were cultured in the presence, or in the absence, of hydroxystearic acid and tested for cell proliferation and viability; the distribution of cells in the cell cycle was evaluated by flow cytometry. Results show that hydroxystearic acid is also an inhibitor of human cell proliferation, and not only of murine C108 cells. Differently from C108 cells, which upon treatment with hydroxystearic acid accumulate in G2-M phases, hydroxystearic acid-treated HT29 cells increase significantly in numbers in G0-G1; I407, embryonic cells used as a control, when treated show only a slight increase in G0-G1. Topics: Adenocarcinoma; Animals; Cell Cycle; Cell Division; Cells, Cultured; Colonic Neoplasms; Depression, Chemical; Humans; Intestines; Lung Neoplasms; Mice; Stearic Acids; Tumor Cells, Cultured	1994

Article

Year

Gas chromatography/mass spectrometric assay of endogenous cellular lipid peroxidation products: quantitative analysis of 9- and 10-hydroxystearic acids.

Rapid communications in mass spectrometry : RCM, 2002, Volume: 16, Issue:9

A sensitive, specific, accurate and reproducible gas chromatography/mass spectrometry method was developed for the assay of 9- and 10-hydroxystearic acids in samples obtained as cell extracts. The preparation of the samples required specific procedures to allow the analysis of both the free and the conjugated hydroxy acids as the corresponding methyl esters. The quantification used propyl-paraben as the internal standard and monitoring of a specific fragment of each isomeric hydroxy acid methyl ester, and allowed quantification of the conjugate and the free fractions of both 9- and 10-hydroxystearic acids. This method is suitable for identification and quantification (LOQ 1.8 and 4.4 ng, respectively) of these important metabolites of lipid peroxidation. In particular the development of an assay for the free 9-hydroxystearic acid methyl ester makes the method a reliable analytical tool for investigations of the role of this metabolite in the mechanisms of tumour cell proliferation.

Topics: Calibration; Carcinoma; Colonic Neoplasms; Gas Chromatography-Mass Spectrometry; Humans; Lipid Peroxidation; Reproducibility of Results; Sensitivity and Specificity; Stearic Acids; Tumor Cells, Cultured

2002

In vitro effects of hydroxystearic acid on the proliferation of HT29 and I407 cells.

Biochemistry and molecular biology international, 1994, Volume: 33, Issue:4

The in vitro effects of hydroxystearic acid on the proliferation of human colon carcinoma cells (HT29) and human embryonic intestine cells (I407) were examined and compared to previous results obtained in murine C108 lung carcinoma cells. The cells were cultured in the presence, or in the absence, of hydroxystearic acid and tested for cell proliferation and viability; the distribution of cells in the cell cycle was evaluated by flow cytometry. Results show that hydroxystearic acid is also an inhibitor of human cell proliferation, and not only of murine C108 cells. Differently from C108 cells, which upon treatment with hydroxystearic acid accumulate in G2-M phases, hydroxystearic acid-treated HT29 cells increase significantly in numbers in G0-G1; I407, embryonic cells used as a control, when treated show only a slight increase in G0-G1.

Topics: Adenocarcinoma; Animals; Cell Cycle; Cell Division; Cells, Cultured; Colonic Neoplasms; Depression, Chemical; Humans; Intestines; Lung Neoplasms; Mice; Stearic Acids; Tumor Cells, Cultured

1994