1-palmitoyl-2-linoleoylphosphatidylcholine has been researched along with Disease-Models--Animal* in 1 studies
1 other study(ies) available for 1-palmitoyl-2-linoleoylphosphatidylcholine and Disease-Models--Animal
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The 5A apolipoprotein A-I mimetic peptide displays antiinflammatory and antioxidant properties in vivo and in vitro.
The apolipoprotein (apo)A-I mimetic peptide 5A is highly specific for ATP-binding cassette transporter (ABC)A1-mediated cholesterol efflux. We investigated whether the 5A peptide shares other beneficial features of apoA-I, such as protection against inflammation and oxidation. Methods- New Zealand white rabbits received an infusion of apoA-I, reconstituted high-density lipoprotein (HDL) containing apoA-I ([A-I]rHDL), or the 5A peptide complexed with phospholipids (1-palmitoyl-2-linoleoyl phosphatidylcholine [PLPC]), before inserting a collar around the carotid artery. Human coronary artery endothelial cells (HCAECs) were incubated with (A-I)rHDL or 5A/PLPC before stimulation with tumor necrosis factor alpha. Results- ApoA-I, (A-I)rHDL, and 5A/PLPC reduced the collar-mediated increase in (1) endothelial expression of cell adhesion molecules vascular cell adhesion molecule-1 and intercellular adhesion molecule-1; (2) production, as well as the expression of the Nox4 catalytic subunits of the NADPH oxidase; and (3) infiltration of circulating neutrophils into the carotid intima-media. In HCAECs, both 5A/PLPC and (A-I)rHDL inhibited tumor necrosis factor-alpha-induced intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 expression, as well as the nuclear factor kappaB signaling cascade and production. The effects of the 5A/PLPC complex were no longer apparent in HCAECs knocked down for ABCA1.. Like apoA-I, the 5A peptide inhibits acute inflammation and oxidative stress in rabbit carotids and HCAECs. In vitro, the 5A peptide exerts these beneficial effects through interaction with ABCA1. Topics: Adult; Animals; Anti-Inflammatory Agents; Antioxidants; Apolipoprotein A-I; ATP Binding Cassette Transporter 1; ATP-Binding Cassette Transporters; Carotid Arteries; Carotid Artery Diseases; Cells, Cultured; Disease Models, Animal; Endothelial Cells; Gene Expression Regulation, Enzymologic; Humans; Intercellular Adhesion Molecule-1; Lipoproteins, HDL; Male; Molecular Mimicry; NADPH Oxidases; Neutrophil Infiltration; NF-kappa B; Peptides; Phosphatidylcholines; Rabbits; Recombinant Proteins; RNA Interference; RNA, Messenger; Signal Transduction; Superoxides; Time Factors; Transfection; Tumor Necrosis Factor-alpha; Vascular Cell Adhesion Molecule-1; Young Adult | 2010 |