1-alpha-24-dihydroxyvitamin-d3 and Kidney-Diseases

An immunosuppressive substance was detected in the serum and plasma of hemodialysis patients. There was a marked increase in its activity immediately after hemodialysis. This substance was not detected in the plasma of hemodialysis patients treated with 1alpha-hydroxycholecalciferol (1alpha-OH-D3), although its disappearance was unrelated to either the total dose or the duration of treatment with 1alpha-OH-D3. During in vitro studies, 1alpha-OH-D3 did not change the phytohemagglutinin-, staphage lysate- and concanavalin A-induced and spontaneous lymphoproliferation of peripheral blood lymphocytes (PBL) from a healthy person. Furthermore, addition of 1alpha-OH-D3 to the plasma of hemodialysis patients who had not received 1alpha-OH-D3 did not interfere with the suppressive activity. On the other hand, 1alpha,24(R)-dihydroxycholecalciferol, 1alpha,24(S)-dihydroxycholecalciferol, and 1alpha,25-dihydroxycholecalciferol, which are all metabolites of 1alpha-OH-D3, suppressed mitogen-induced lymphoproliferation of PBL from a healthy person.

Topics: Adult; Aged; Calcitriol; Dihydroxycholecalciferols; DNA; Female; Humans; Hydroxycholecalciferols; Immunity; Immunosuppressive Agents; Kidney Diseases; Lymphocytes; Male; Middle Aged; Renal Dialysis