1-3-dihydroxy-4-4-5-5-tetramethyl-2-(4-carboxyphenyl)tetrahydroimidazole and Neurodegenerative-Diseases

1-3-dihydroxy-4-4-5-5-tetramethyl-2-(4-carboxyphenyl)tetrahydroimidazole has been researched along with Neurodegenerative-Diseases* in 1 studies

Other Studies

1 other study(ies) available for 1-3-dihydroxy-4-4-5-5-tetramethyl-2-(4-carboxyphenyl)tetrahydroimidazole and Neurodegenerative-Diseases

ArticleYear
Superoxide-induced nitric oxide release from cultured glial cells.
    Brain research, 2001, Aug-24, Volume: 911, Issue:2

    Nitric oxide (NO) has been implicated as a potential contributor to neural cell death in a variety of neurological conditions. Cultured glial cells were exposed to extracellular superoxide generated by the action of xanthine oxidase on xanthine. In this experimental paradigm, both C6 glioma cells and primary astrocytes from rat cerebral cortex produced a rapid release of nitric oxide, measured using an NO specific electrode, in response to the applied superoxide stimulus. Application of a superoxide scavenger, or over-expression of Cu/Zn superoxide dismutase decreased the observed NO release. Authenticity of the NO signal was confirmed by the addition of the NO scavenger 2-(carboxyphenyl)-4,4,5,5-tetramethyllimidazoline-1-oxyl 3-oxide (carboxy-PTIO), which abolished the observed NO release without affecting simultaneously measured superoxide. Therefore, we suggest that glial cells may produce NO under free radical stimulation, which may be relevant to several neurological disorders where superoxide radicals are generated in the vicinity of glia. This would be predicted to result in the release of NO, which may exert toxic effects on neighbouring cells.

    Topics: Animals; Astrocytes; Benzoates; Extracellular Space; Free Radical Scavengers; Gene Expression Regulation; Genetic Vectors; Imidazoles; Metalloporphyrins; Nerve Degeneration; Neurodegenerative Diseases; Neurons; Nitric Oxide; Nitric Oxide Synthase; Oxidative Stress; Rats; RNA, Messenger; Superoxide Dismutase; Superoxide Dismutase-1; Superoxides; Transfection; Tumor Cells, Cultured; Xanthine; Xanthine Oxidase

2001