Compounds > 1-1--(4-4-7-7-tetramethyl-4-7-diazaundecamethylene)bis-4-(3-methyl-2-3-dihydro(benzo-1-3-thiazole)-2-methylidene)quinolinium

1-1--(4-4-7-7-tetramethyl-4-7-diazaundecamethylene)bis-4-(3-methyl-2-3-dihydro(benzo-1-3-thiazole)-2-methylidene)quinolinium and Cystic-Fibrosis

1-1--(4-4-7-7-tetramethyl-4-7-diazaundecamethylene)bis-4-(3-methyl-2-3-dihydro(benzo-1-3-thiazole)-2-methylidene)quinolinium has been researched along with Cystic-Fibrosis* in 1 studies

Other Studies

1 other study(ies) available for 1-1--(4-4-7-7-tetramethyl-4-7-diazaundecamethylene)bis-4-(3-methyl-2-3-dihydro(benzo-1-3-thiazole)-2-methylidene)quinolinium and Cystic-Fibrosis

Article	Year
Use of stable dye-DNA intercalating complexes to detect cystic fibrosis mutations. Molecular and cellular probes, 1994, Volume: 8, Issue:3 Several dyes are now known which intercalate into double-stranded DNA with fluorescence enhancement. One such is TOTO (1,1'-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)-bis-4-[3-meth yl-2,3- dihydro-(benzo-1,3-thiazole)-2-methlyidene]-quinolinium tetraiodide), a homodimer of thiazole orange, whose complexes with DNA are stable to electrophoresis and show a 1000-fold enhancement after binding. We demonstrate that this reagent can be successfully used in combination with a laser-excited fluorescence gel scanner to detect the three most common cystic fibrosis mutant alleles, delta F508, G551D and G542X. This has potential application to mass population heterozygote screening. Topics: Alleles; Base Sequence; Cystic Fibrosis; DNA; Fluorescence; Heterozygote; Humans; Mass Screening; Molecular Sequence Data; Mutation; Polymerase Chain Reaction; Protein Binding; Quinolinium Compounds; Thiazoles	1994

Article

Year

Use of stable dye-DNA intercalating complexes to detect cystic fibrosis mutations.

Molecular and cellular probes, 1994, Volume: 8, Issue:3

Several dyes are now known which intercalate into double-stranded DNA with fluorescence enhancement. One such is TOTO (1,1'-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)-bis-4-[3-meth yl-2,3- dihydro-(benzo-1,3-thiazole)-2-methlyidene]-quinolinium tetraiodide), a homodimer of thiazole orange, whose complexes with DNA are stable to electrophoresis and show a 1000-fold enhancement after binding. We demonstrate that this reagent can be successfully used in combination with a laser-excited fluorescence gel scanner to detect the three most common cystic fibrosis mutant alleles, delta F508, G551D and G542X. This has potential application to mass population heterozygote screening.

Topics: Alleles; Base Sequence; Cystic Fibrosis; DNA; Fluorescence; Heterozygote; Humans; Mass Screening; Molecular Sequence Data; Mutation; Polymerase Chain Reaction; Protein Binding; Quinolinium Compounds; Thiazoles

1994