Compounds > 1-1--((4-4-7-7-tetramethyl)-4-7-diazaundecamethylene)bis-4-(3-methyl-2-3-dihydro(benzo-1-3-oxazole)-2-methylidine)quinolinium--tetraiodide

1-1--((4-4-7-7-tetramethyl)-4-7-diazaundecamethylene)bis-4-(3-methyl-2-3-dihydro(benzo-1-3-oxazole)-2-methylidine)quinolinium--tetraiodide and Necrosis

1-1--((4-4-7-7-tetramethyl)-4-7-diazaundecamethylene)bis-4-(3-methyl-2-3-dihydro(benzo-1-3-oxazole)-2-methylidine)quinolinium--tetraiodide has been researched along with Necrosis* in 1 studies

Other Studies

1 other study(ies) available for 1-1--((4-4-7-7-tetramethyl)-4-7-diazaundecamethylene)bis-4-(3-methyl-2-3-dihydro(benzo-1-3-oxazole)-2-methylidine)quinolinium--tetraiodide and Necrosis

Article	Year
Apoptosis assessment by the DNA diffusion assay. Methods in molecular medicine, 2005, Volume: 111 A simple, sensitive, and reliable DNA diffusion assay for quantification of apoptosis is based on the principle that nuclear DNA of apoptotic cells have abundant alkali-labile sites and under alkaline conditions small pieces of DNA thus generated diffuse in agarose, giving the appearance of a halo if stained with a sensitive fluorescent dye such as YOYO-1. The protocol for detection of apoptosis described here is tested for estimation of apoptosis in human leukocytes (mostly neutrophils) incubated at 37 degrees C for 24 h. Cells were mixed with agarose, microgels were made, and cells were lysed in a solution of high salt, detergents, and alkali. DNA was precipitated in microgels by ethanol and spermine. Staining of DNA was done with an intense fluorescent dye, YOYO-1. Apoptotic cells show a circular gradient of granular DNA with a dense central zone and a lighter and hazy outer zone, giving the overall appearance of a halo. Topics: Animals; Apoptosis; Benzoxazoles; Brain; Cytological Techniques; Diffusion; DNA; Fluorescent Dyes; Humans; Leukocytes; Male; Necrosis; Quinolinium Compounds; Rats; Sepharose; Spermatozoa; Temperature; Thymus Gland; Time Factors	2005

Article

Year

Apoptosis assessment by the DNA diffusion assay.

Methods in molecular medicine, 2005, Volume: 111

A simple, sensitive, and reliable DNA diffusion assay for quantification of apoptosis is based on the principle that nuclear DNA of apoptotic cells have abundant alkali-labile sites and under alkaline conditions small pieces of DNA thus generated diffuse in agarose, giving the appearance of a halo if stained with a sensitive fluorescent dye such as YOYO-1. The protocol for detection of apoptosis described here is tested for estimation of apoptosis in human leukocytes (mostly neutrophils) incubated at 37 degrees C for 24 h. Cells were mixed with agarose, microgels were made, and cells were lysed in a solution of high salt, detergents, and alkali. DNA was precipitated in microgels by ethanol and spermine. Staining of DNA was done with an intense fluorescent dye, YOYO-1. Apoptotic cells show a circular gradient of granular DNA with a dense central zone and a lighter and hazy outer zone, giving the overall appearance of a halo.

Topics: Animals; Apoptosis; Benzoxazoles; Brain; Cytological Techniques; Diffusion; DNA; Fluorescent Dyes; Humans; Leukocytes; Male; Necrosis; Quinolinium Compounds; Rats; Sepharose; Spermatozoa; Temperature; Thymus Gland; Time Factors

2005