(4-24)-ply(a) and HIV-Infections

(4-24)-ply(a) has been researched along with HIV-Infections* in 1 studies

Other Studies

1 other study(ies) available for (4-24)-ply(a) and HIV-Infections

ArticleYear
Targeted delivery of PSC-RANTES for HIV-1 prevention using biodegradable nanoparticles.
    Pharmaceutical research, 2009, Volume: 26, Issue:3

    Nanoparticles formulated from the biodegradable co-polymer poly(lactic-co-glycolic acid) (PLGA), were investigated as a drug delivery system to enhance tissue uptake, permeation, and targeting for PSC-RANTES anti-HIV-1 activity.. PSC-RANTES nanoparticles formulated via a double emulsion process and characterized in both in vitro and ex vivo systems to determine PSC-RANTES release rate, nanoparticle tissue permeation, and anti-HIV bioactivity.. Spherical, monodisperse (PDI = 0.098 +/- 0.054) PSC-RANTES nanoparticles (d = 256.58 +/- 19.57 nm) with an encapsulation efficiency of 82.23 +/- 8.35% were manufactured. In vitro release studies demonstrated a controlled release profile of PSC-RANTES (71.48 +/- 5.25% release). PSC-RANTES nanoparticle maintained comparable anti-HIV activity with unformulated PSC-RANTES in a HeLa cell-based system with an IC(50) of approximately 1pM. In an ex vivo cervical tissue model, PSC-RANTES nanoparticles displayed a fivefold increase in tissue uptake, enhanced tissue permeation, and significant localization at the basal layers of the epithelium over unformulated PSC-RANTES.. These results indicate that PSC-RANTES can readily be encapsulated into a PLGA nanoparticle drug delivery system, retain its anti-HIV-1 activity, and deliver PSC-RANTES to the target tissue. This is crucial for the success of this drug candidate as a topical microbicide product.

    Topics: Adult; Antimicrobial Cationic Peptides; Biocompatible Materials; Cervix Uteri; Chemokine CCL5; Drug Carriers; Female; HeLa Cells; HIV Fusion Inhibitors; HIV Infections; HIV-1; Humans; In Vitro Techniques; Middle Aged; Nanoparticles; Permeability; Solubility; Surface Properties; Virus Cultivation

2009