(1-(3-isopropyl-1-2-4-oxadiazol-5-yl)piperidin-4-yl)methyl-methanesulfonate and Non-alcoholic-Fatty-Liver-Disease

(1-(3-isopropyl-1-2-4-oxadiazol-5-yl)piperidin-4-yl)methyl-methanesulfonate has been researched along with Non-alcoholic-Fatty-Liver-Disease* in 2 studies

Other Studies

2 other study(ies) available for (1-(3-isopropyl-1-2-4-oxadiazol-5-yl)piperidin-4-yl)methyl-methanesulfonate and Non-alcoholic-Fatty-Liver-Disease

ArticleYear
APD668, a G protein-coupled receptor 119 agonist improves fat tolerance and attenuates fatty liver in high-trans fat diet induced steatohepatitis model in C57BL/6 mice.
    European journal of pharmacology, 2017, Apr-15, Volume: 801

    G-protein coupled receptor 119 (GPR119) receptor is a rhodopsin-like, class A Gαs-coupled receptor, predominantly expressed in pancreatic islet cells and intestinal entero-endocrine cells. GPR119 has been emerged as a novel therapeutic target for the treatment of dyslipidemia in type 2 diabetes. In this study, we investigated the effect of APD668, a GPR119 agonist alone and in combination with linagliptin, a DPPIV inhibitor on oral fat tolerance test. Our findings demonstrate that APD668, a GPR119 agonist inhibits the intestinal triglyceride absorption after acute fat load in mice. Single dose administration of APD668 increases incretin secretion and enhances total PYY levels in presence of fat load in mice. We found that, the anti-dyslipidemic action of APD668 was reversed in presence of exendin-3 in oral fat tolerance test. In addition, our results showed that exendin-3 (9-39) failed to block the effect of APD668 on gastric emptying indicating that gastric emptying effects of APD668 are indeed mediated through GPR119 receptor dependent mechanism. Combined administration of APD668 and linagliptin significantly increased plasma active GLP-1 levels in-vivo and showed improvement in fat tolerance. However, APD668 failed to show anti-dyslipidemic activity in tyloxapol-induced hyperlipidemia in mice. Furthermore, we investigated the chronic effects of APD668 on hepatic steatosis in high trans-fat diet fed steatohepatitis model in mice. Oral administration of APD668 in HTF diet fed mice ameliorated hepatic endpoints such as plasma ALT, AST, liver weight and steatosis. These findings suggest that GPR119 agonists may represent a promising therapeutic strategy for the treatment of dyslipidemia and non-alcoholic steatohepatitis.

    Topics: Animals; Diet, High-Fat; Drug Interactions; Gastric Emptying; Glucagon-Like Peptide 1; Glucagon-Like Peptide-1 Receptor; Linagliptin; Male; Mesylates; Mice; Mice, Inbred C57BL; Non-alcoholic Fatty Liver Disease; Oxadiazoles; Polyethylene Glycols; Pyrazoles; Pyrimidines; Receptors, G-Protein-Coupled; Tetrazoles; Thiazoles; Triglycerides

2017
GPR119: a promising target for nonalcoholic fatty liver disease.
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2016, Volume: 30, Issue:1

    Nonalcoholic fatty liver disease is associated with metabolic syndrome and has the unique characteristic of excess lipid accumulation in liver. G-protein-coupled receptor 119 (GPR119) is a promising target for type 2 diabetes. However, the role of GPR119 activation in hepatic steatosis and its precise mechanism has not been investigated. In primary cultured hepatocytes from wild-type and GPR119 knockout (KO) mice, expression of lipogenic enzymes was elevated in GPR119 KO hepatocytes. Treatment of hepatocytes and HepG2 cells with GPR119 agonists in phase 2 clinical trials (MBX-2982 [MBX] and GSK1292263) inhibited protein expression of both nuclear and total sterol regulatory element binding protein (SREBP)-1, a key lipogenesis transcription factor. Oral administration of MBX in mice fed a high-fat diet potently inhibited hepatic lipid accumulation and expression levels of SREBP-1 and lipogenesis-related genes, whereas the hepatic antilipogenesis effects of MBX were abolished in GPR119 KO mice. MBX activated AMPK and increased Ser-372 phosphorylation of SREBP-1c, an inhibitory form of SREBP-1c. Moreover, inhibition of AMPK recovered MBX-induced down-regulation of SREBP-1. These findings demonstrate for the first time that the GPR119 ligand alleviates hepatic steatosis by inhibiting SREBP-1-mediated lipogenesis in hepatocytes.

    Topics: AMP-Activated Protein Kinases; Animals; Cells, Cultured; Hep G2 Cells; Hepatocytes; Humans; Mesylates; Mice; Mice, Inbred C57BL; Non-alcoholic Fatty Liver Disease; Oxadiazoles; Receptors, G-Protein-Coupled; Sterol Regulatory Element Binding Protein 1; Tetrazoles; Thiazoles

2016