znaf-2 and calcium-orange

znaf-2 has been researched along with calcium-orange* in 1 studies

Other Studies

1 other study(ies) available for znaf-2 and calcium-orange

ArticleYear
Negative modulation of presynaptic activity by zinc released from Schaffer collaterals.
    Journal of neuroscience research, 2007, Volume: 85, Issue:16

    The role of zinc in excitation of Schaffer collateral-CA1 pyramidal cell synapses is poorly understood. Schaffer collaterals stained with ZnAF-2 or ZnAF-2DA, a membrane-impermeable or a membrane-permeable zinc indicator, respectively, were treated by tetanic stimulation (200 Hz, 1 sec). Extracellular and intracellular ZnAF-2 signals were increased in the stratum radiatum of the CA1, in which Schaffer collateral synapses exist. Both the increases were completely blocked in the presence of 1 mM CaEDAT, a membrane-impermeable zinc chelator, suggesting that 1 mM CaEDTA is effective for chelating zinc released from Schaffer collaterals. The role of Schaffer collateral zinc in presynaptic activity was examined by using FM4-64, a fluorescent indicator for vesicular exocytosis. The decrease in FM4-64 signal during tetanic stimulation (10 Hz, 180 sec) was enhanced in Schaffer collaterals in the presence of 1 mM CaEDTA but suppressed in the presence of 5 microM ZnC1(2), suggesting that zinc released from Schaffer collaterals suppresses presynaptic activity during tetanic stimulation. When Schaffer collateral synapses stained with calcium orange AM, a membrane-permeable calcium indicator, were regionally stimulated with 1 mM glutamate, calcium orange signal was increased in the CA1 pyramidal cell layer. This increase was enhanced in the presence of CaEDTA and attenuated in the presence of zinc. These results suggest that zinc attenuates excitation of Schaffer collateral synapses elicited with glutamate via suppression of presynaptic activity.

    Topics: Animals; Axons; Calcium; Calcium Signaling; Chelating Agents; Coloring Agents; Electric Stimulation; Glutamic Acid; Hippocampus; Neural Inhibition; Organ Culture Techniques; Organic Chemicals; Presynaptic Terminals; Pyridines; Pyridinium Compounds; Quaternary Ammonium Compounds; Rats; Rats, Wistar; Synaptic Transmission; Up-Regulation; Zinc

2007