zinostatin and mastoparan

It has been suggested that G0 and Gi play a role in the collapse of axonal growth cones and the retraction of neurites. We have studied the G-protein content of neuroblastoma cells undergoing neurite outgrowth and subsequent retraction in response to neocarzinostatin (NCS). Stimulators of G0 and Gi have no effect upon neurite outgrowth or retraction and the cellular content of G0 and Gi does not change during the course of these morphological phenomena. Modulation of G-protein content, therefore, most likely does not play a role in this process.

Topics: Animals; Blotting, Western; Cells, Cultured; Dose-Response Relationship, Drug; Gene Expression; GTP-Binding Proteins; Intercellular Signaling Peptides and Proteins; Neurites; Neuroblastoma; Peptides; Wasp Venoms; Zinostatin