zearalenone and 4-phenylbutylamine

Zearalenone (ZEA) is an estrogen-like mycotoxin, which mainly led to reproductive toxicity. The study aimed to investigate the molecular mechanism of ZEA-induced dysfunction of mitochondria-associated endoplasmic reticulum membranes (MAM) in piglet Sertoli cells (SCs) via the endoplasmic reticulum stress (ERS) pathway. In this study, SCs were used as a research object that was exposed to ZEA, and ERS inhibitor 4-Phenylbutyrate acid (4-PBA) was used as a reference. The results showed that ZEA damaged cell viability and increased Ca

Topics: Animals; Endoplasmic Reticulum; Endoplasmic Reticulum Stress; Male; Mitochondria; RNA, Messenger; Sertoli Cells; Swine; Zearalenone

Zearalenone (ZEA) and deoxynivalenol (DON) are widely found in various feeds, which harms livestock's reproductive health. Both mitochondria and endoplasmic reticulum (ER) can regulate cell apoptosis. This study aimed to explore the regulatory mechanism of endoplasmic reticulum stress (ERS) on ZEA- combined with DON-induced mitochondrial pathway apoptosis in piglet Sertoli cells (SCs). The results showed that ZEA + DON damaged the ultrastructure of the cells, induced apoptosis, decreased mitochondrial membrane potential, promoted the expression of cytochrome c (CytC), and decreased the cell survival rate. Furthermore, ZEA + DON increased the relative mRNA and protein expression of

Topics: Animals; Apoptosis; Caspase 3; Endoplasmic Reticulum Stress; Male; Mitochondria; Proto-Oncogene Proteins c-bcl-2; RNA, Messenger; Sertoli Cells; Swine; Tumor Suppressor Protein p53; Zearalenone

Topics: Animals; Apoptosis; bcl-2-Associated X Protein; Caspase 3; Caspase 9; Endoplasmic Reticulum Stress; MAP Kinase Signaling System; Proto-Oncogene Proteins c-bcl-2; Stromal Cells; Swine; Zearalenone