zearalenone and 1-naphthol

zearalenone has been researched along with 1-naphthol* in 2 studies

Other Studies

2 other study(ies) available for zearalenone and 1-naphthol

Article	Year
Effect of UDP-glucuronyltransferase induction on zearalenone metabolism. Toxicology letters, 1990, Volume: 51, Issue:2 Experiments were conducted to determine the UDP-glucuronyltransferase (UDP-GT) isoenzyme which catalyzes zearalenone (Z) conjugation, and the effect of increased enzyme activity on Z metabolism. In competitive enzyme assays, the activity of rat liver UDP-GT towards Z was inhibited by 1-naphthol (NA), a GT1 substrate, and 4-hydroxybiphenyl (HB), a GT2 substrate. When enzyme activity was induced with either 3-methylcholanthrene (MC), a GT1 inducer, or phenobarbital (PB), a GT2 inducer, increased UDP-GT activity towards Z, NA and HB was observed. UDP-GT induction by PB increased urinary excretion of conjugated alpha-zearalenol. These results indicate that UDP-GT isoenzymes have overlapping substrate specificities, and that Z detoxification may be enhanced by UDP-GT enzyme induction, resulting in increased urinary excretion of conjugated alpha-zearalenol. Topics: Animals; Biphenyl Compounds; Enzyme Induction; Female; Glucuronosyltransferase; Inactivation, Metabolic; Isoenzymes; Liver; Methylcholanthrene; Naphthols; Phenobarbital; Rats; Rats, Inbred Strains; Resorcinols; Zearalenone	1990
Metabolism of zearalenone by sow intestinal mucosa in vitro. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 1987, Volume: 25, Issue:9 Homogenized intestinal mucosa samples from sows were incubated with zearalenone in the presence of NADPH or UDPGA. In addition, UDPglucuronosyltransferase activity in the microsomal fraction of mucosa was determined using 1-naphthol as substrate. In the presence of NADPH, zearalenone was reduced to both alpha- and beta-zearalenol (0.37 +/- 0.18 and 0.29 +/- 0.11 nmol/mg protein/hr in the duodenum and jejunum, respectively). The beta-isomer was the predominant metabolite. Glucuronide conjugation of zearalenone was very high compared with the level of reduction occurring (11.3 +/- 6.1 and 9.4 +/- 5.8 nmol conjugated/mg protein/hr in the duodenum and jejunum, respectively). There was no correlation between the rates of glucuronide conjugation of zearalenone and 1-naphthol, indicating that they depend upon two different isoenzymes of UDPglucuronosyltransferase. Topics: Animals; Duodenum; Female; Glucuronates; Glucuronosyltransferase; Intestinal Mucosa; Jejunum; Microsomes; NADP; Naphthols; Resorcinols; Swine; Uridine Diphosphate Glucuronic Acid; Zearalenone	1987

Article

Year

Effect of UDP-glucuronyltransferase induction on zearalenone metabolism.

Toxicology letters, 1990, Volume: 51, Issue:2

Experiments were conducted to determine the UDP-glucuronyltransferase (UDP-GT) isoenzyme which catalyzes zearalenone (Z) conjugation, and the effect of increased enzyme activity on Z metabolism. In competitive enzyme assays, the activity of rat liver UDP-GT towards Z was inhibited by 1-naphthol (NA), a GT1 substrate, and 4-hydroxybiphenyl (HB), a GT2 substrate. When enzyme activity was induced with either 3-methylcholanthrene (MC), a GT1 inducer, or phenobarbital (PB), a GT2 inducer, increased UDP-GT activity towards Z, NA and HB was observed. UDP-GT induction by PB increased urinary excretion of conjugated alpha-zearalenol. These results indicate that UDP-GT isoenzymes have overlapping substrate specificities, and that Z detoxification may be enhanced by UDP-GT enzyme induction, resulting in increased urinary excretion of conjugated alpha-zearalenol.

Topics: Animals; Biphenyl Compounds; Enzyme Induction; Female; Glucuronosyltransferase; Inactivation, Metabolic; Isoenzymes; Liver; Methylcholanthrene; Naphthols; Phenobarbital; Rats; Rats, Inbred Strains; Resorcinols; Zearalenone

1990

Metabolism of zearalenone by sow intestinal mucosa in vitro.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 1987, Volume: 25, Issue:9

Homogenized intestinal mucosa samples from sows were incubated with zearalenone in the presence of NADPH or UDPGA. In addition, UDPglucuronosyltransferase activity in the microsomal fraction of mucosa was determined using 1-naphthol as substrate. In the presence of NADPH, zearalenone was reduced to both alpha- and beta-zearalenol (0.37 +/- 0.18 and 0.29 +/- 0.11 nmol/mg protein/hr in the duodenum and jejunum, respectively). The beta-isomer was the predominant metabolite. Glucuronide conjugation of zearalenone was very high compared with the level of reduction occurring (11.3 +/- 6.1 and 9.4 +/- 5.8 nmol conjugated/mg protein/hr in the duodenum and jejunum, respectively). There was no correlation between the rates of glucuronide conjugation of zearalenone and 1-naphthol, indicating that they depend upon two different isoenzymes of UDPglucuronosyltransferase.

Topics: Animals; Duodenum; Female; Glucuronates; Glucuronosyltransferase; Intestinal Mucosa; Jejunum; Microsomes; NADP; Naphthols; Resorcinols; Swine; Uridine Diphosphate Glucuronic Acid; Zearalenone

1987