warfarin and dorzolamide

warfarin has been researched along with dorzolamide* in 1 studies

Other Studies

1 other study(ies) available for warfarin and dorzolamide

ArticleYear
Detection of protein-small molecule binding using a self-referencing external cavity laser biosensor.
    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference, 2014, Volume: 2014

    High throughput screening of protein-small molecule binding interactions using label-free optical biosensors is challenging, as the detected signals are often similar in magnitude to experimental noise. Here, we describe a novel self-referencing external cavity laser (ECL) biosensor approach that achieves high resolution and high sensitivity, while eliminating thermal noise with sub-picometer wavelength accuracy. Using the self-referencing ECL biosensor, we demonstrate detection of binding between small molecules and a variety of immobilized protein targets with binding affinities or inhibition constants in the sub-nanomolar to low micromolar range. The demonstrated ability to perform detection in the presence of several interfering compounds opens the potential for increasing the throughput of the approach. As an example application, we performed a "needle-in-the-haystack" screen for inhibitors against carbonic anhydrase isozyme II (CA II), in which known inhibitors are clearly differentiated from inactive molecules within a compound library.

    Topics: Biosensing Techniques; Carbonic Anhydrase II; Enzymes, Immobilized; Humans; Indoles; Lasers; Protein Binding; Serum Albumin; Small Molecule Libraries; Sulfonamides; Thiophenes; Warfarin

2014