warfarin and 1-1-diphenyl-2-picrylhydrazyl

The stability of solutions of fullerene C₆₀ with human serum albumin (C₆₀/HSA) has not been studied in detail. In this study, we report on the preparation of stable C₆₀/HSA solutions that are formed via the formation of C₆₀/HP-β-CyD nanoparticles, i.e. by transferring C₆₀ molecules from HP-β-CyD to HSA molecules, and an investigation of the reactive oxygen species on the behavior of the resulting C₆₀/HSA.. Structural and functional properties were examined by spectroscopic techniques, including circular dichroism (CD), fluorescent spectra (FI), and electron spin resonance spin-trapping method, and by cell viability test using A549 cells.. Aqueous C₆₀/HSA solutions with a small distribution size, excellent dispersion stability and a high dispersion concentration were obtained without the use of organic solvents. CD and FI spectra indicated that the HSA had undergone structural changes as the result of C₆₀/HSA formation. Binding experiments indicated that C₆₀/HSA had increased Site I and Site II-ligand binding capabilities. The HSA, in the form of C₆₀/HSA was, therefore, conformationally altered, and its binding capabilities were enhanced. Radical scavenging studies using the 1,1'-diphenyl-2-picrylhydrazyl radical showed that C₆₀/HSA had an increased antioxidant activity, compared to HSA alone. Further, C₆₀/HSA efficiently generated not only superoxide anion radicals O₂·- but also singlet oxygen ¹O₂ through photoirradiation. C₆₀/HSA showed cell toxicity characteristics after light irradiation, but no toxicity was observed in the absence of irradiation.. C₆₀/HSA not only has an excellent stability and antioxidant activity, but also has substantial phototoxicity properties. It thus appears that HSA can be used as a carrier of C₆₀in drug delivery systems for photodynamic therapy.

Topics: Amino Acids, Aromatic; Anti-Inflammatory Agents, Non-Steroidal; Antioxidants; Biphenyl Compounds; Cell Line, Tumor; Circular Dichroism; Cyclodextrins; Cytochromes c; Dermatitis, Phototoxic; Drug Stability; Free Radical Scavengers; Fullerenes; Humans; Ketoprofen; Ligands; Nanoparticles; Picrates; Reactive Oxygen Species; Serum Albumin; Solubility; Warfarin