vitamin-k-semiquinone-radical has been researched along with peroxynitric-acid* in 1 studies
1 other study(ies) available for vitamin-k-semiquinone-radical and peroxynitric-acid
| Article | Year |
|---|---|
Decrease in accessible thiols as an index of oxidative damage to membrane proteins.
The effect of several oxidative agents (hydrogen peroxide, tert-butyl hydroperoxide, menadione, AAPH, peroxynitrite and ionizing radiation) on the ratio of weakly to strongly immobilized residues of erythrocyte membrane-bound maleimide-tempo spin label (h(w)/h(s) ratio) was studied in order to test the hypothesis that a decrease in the h(w)/h(s) ratio may be a general index of oxidative damage to membrane proteins. Most of the agents studied decreased though H2O2 almost did not affect and ionizing radiation increased the h(w)/h(s) ratio. In parallel, the ratio of DTNB accessible/DTNB inaccessible membrane protein-SH groups was determined from membrane-SH group measurements with the Ellman reagent in the absence and in the presence of sodium dodecyl sulfate. This ratio decreased in all cases studied and seems to be a more universal and easy to measure parameter to describe the oxidative damage to membrane proteins. Topics: Adult; Amidines; Cyclic N-Oxides; Dithionitrobenzoic Acid; Electron Spin Resonance Spectroscopy; Erythrocyte Membrane; Gamma Rays; Humans; Hydrogen Peroxide; Membrane Proteins; Nitrates; Oxidants; Oxidative Stress; Peroxides; Spin Labels; Sulfhydryl Compounds; tert-Butylhydroperoxide; Vitamin K | 1997 |