vitamin-k-semiquinone-radical has been researched along with 5--(4-fluorosulfonylbenzoyl)adenosine* in 1 studies
1 other study(ies) available for vitamin-k-semiquinone-radical and 5--(4-fluorosulfonylbenzoyl)adenosine
| Article | Year |
|---|---|
Suggested mechanism for the modulation of the activity of NAD(P)H:quinone acceptor oxidoreductase (DT-diaphorase) by menadione: interpretation of the effect of menadione on 5'-[p-(Fluorosulfonyl)benzoyl]adenosine labeling of rat liver NAD(P)H:quinone acce
5'-[p-(Fluorosulfonyl)benzoyl]adenosine (5'FSBA) was previously shown to be an active site-directed affinity label of rat liver NAD(P)H:quinone acceptor oxidoreductase [Mol. Pharmacol. 35:818-822 (1989)]. Our recent study revealed that menadione, the substrate of this quinone reductase, had a magnifying effect on inactivation of the enzyme by 5'-FSBA. The dissociation constant for the initial reversible enzyme-inhibitor complex was significantly lower and the rate of inactivation was increased when menadione was present during the incubation. However, [14C]5'FSBA labeling was reduced in the presence of menadione. These results are presented and a possible mechanism for the enzyme is discussed. Topics: Adenosine; Affinity Labels; Animals; Enzyme Activation; Female; Kinetics; Liver; NAD(P)H Dehydrogenase (Quinone); Rats; Rats, Wistar; Vitamin K | 1992 |