vitamin-k-semiquinone-radical has been researched along with 25-26-dihydroxycholecalciferol* in 1 studies
1 other study(ies) available for vitamin-k-semiquinone-radical and 25-26-dihydroxycholecalciferol
| Article | Year |
|---|---|
Quantitation of the main metabolites of vitamin D in a single serum sample. I. Extraction, separation and purification of metabolites.
A method for extraction, separation and purification of the main serum metabolites of vitamin D from a single serum sample is described. The method involved extraction of serum by diethylether and separation and purification of vitamin D, 25-OHD and the dihydroxymetabolites 24,25-(OH)2D, 25,26-(OH),2D and 1,25-(OH)2D by elution in three steps from a short open silicic acid column. The eluted vitamin D metabolites were further separated and purified by high pressure liquid chromatography (HPLC). The HPLC systems described separated the D2 and D3 forms of vitamin D, 25-OHD, 1,25-(OH)2D, and probably also 24,25-(OH)2D and 25,26-(OH)2D. The metabolites were purified by the methods described for further quantitation by UV-absorption or competitive protein binding assays, and were found to be homogenous on re-chromatography with different HPLC systems. Good recoveries were obtained for all the metabolites. Topics: 24,25-Dihydroxyvitamin D 3; Calcifediol; Calcitriol; Chromatography, High Pressure Liquid; Dihydroxycholecalciferols; Humans; Hydroxycholecalciferols; Silicic Acid; Vitamin D; Vitamin K | 1980 |