vincosamide and pumiloside

vincosamide has been researched along with pumiloside* in 1 studies

Other Studies

1 other study(ies) available for vincosamide and pumiloside

ArticleYear
Biosynthesis-inspired mining and identification of untapped alkaloids in Camptotheca acuminate for enzyme discovery using ultra-high performance liquid chromatography coupled with quadrupole-time of flight-mass spectrometry.
    Journal of chromatography. A, 2020, Jun-07, Volume: 1620

    Leaves, flowers, fruits and stems (44 sample groups) were collected from mature Camptotheca acuminate during 2017.3-2018.3 and classified by ultra-high performance liquid chromatography coupled with quadrupole-time of flight-mass spectrometry based metabolomics. One hundred metabolites including forty-seven alkaloids, fifteen terpenes, thirty-two polyphenols and six other metabolites were rapidly identified through the in-house database alignment at first glance. Thirty-three alkaloids classified into five groups including camptothecin group (CG1-13), pumiloside group (PG1-5), strictosidinic acid group (SG1-3), vincosamide group (VG1-7), and a new hybrid group, vincosamide-camptothecin group (VC1-5) were mined and further characterized by MS/MS analyses. The identification of two untapped biosynthetic precursors, 2-hydroxypumiloside (PG2) and 16‑hydroxy‑15, 16-dihydrocamptothecoside (CG3), along with sixteen new alkaloids enables us for a better understanding of camptothecin biogenetic reasoning. The underlying enzymes involved in camptothecin biosynthesis were also proposed according to the guiding metabolic map, thus purposefully mining of enzymes involved in the downstream biosynthetic pathway of camptothecin could be initiated with the help of this map.

    Topics: Alkaloids; Biosynthetic Pathways; Camptotheca; Camptothecin; Carbolines; Chromatography, High Pressure Liquid; Databases as Topic; Discriminant Analysis; Glycosides; Indole Alkaloids; Least-Squares Analysis; Metabolic Networks and Pathways; Metabolome; Metabolomics; Multivariate Analysis; Principal Component Analysis; Tandem Mass Spectrometry

2020