vicenin-ii and isoschaftoside

An HPLC method for combined quantification of five major constituents (vicenin-1, schaftoside, isoschaftoside, vicenin-3 and isovitexin) of Desmodium styracifolium was developed to evaluate the quality consistency of this medicinal herb. The chromatographic separation was accomplished on an Agilent 5 TC-C18 column (4.6 × 250 mm, 5 μm) with gradient elution using acetonitrile and 0.1 % aqueous formic acid (v/v). The column temperature was 30 °C and the detection wavelength was 272 nm. The chromatographic data were analyzed with a novel systematic quantitative fingerprint method (SQFM). The established quantitative fingerprint method was successfully applied to simultaneously determine the levels of the major constituents, and 39 common peaks were found. The samples collected in Guangdong province had a good quality consistency, in accordance with the traditional opinion that Guangdong province is the best region for cultivation of D. styracifolium. The fingerprint-antioxidant efficacy relationship of the different samples was investigated by examining the correlation between the common peaks and the antioxidant effect. Twenty-two common peaks were positively correlated with the antioxidant effect, while the others showed a negative correlation. This quantitative fingerprint method could be considered as a suitable approach to evaluate the quality of traditional Chinese medicines and herbal preparations. The SQFM was reliable and efficient for analysis of the chromatographic data.

Topics: Acetonitriles; Antioxidants; Apigenin; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; Fabaceae; Glucosides; Glycosides; Plant Extracts

Abrus mollis, a commonly used traditional Chinese medicine in China and other Asia countries, has been used clinically to prevent and treat hepatitis and alcoholic liver disease for decades.. A modified HPLC-MS method was developed for the determination of vicenin-2 (AM-I), isoschaftoside (AM-II), and schaftoside (AM-III) of AM extract (AME) in rat plasma and tissues (heart, liver, spleen, lungs, and kidneys). Following oral administration of AME to rat at a dose of 200mg/kg, the concentrations of AM-I, II and III in plasma and tissues were quantified. An integrated double peak pharmacokinetics model was used to fit the concentration-time curves. The effects of drug on the bile flow and toe swelling of rats induced by carrageenan were also studied.. The limit of quantitation of this modified HPLC-MS method decreased from 25 to 5ng/mL for plasma and from 100 to 10ng/g for tissue. These concentration-time curves show two successive maximum concentrations. The results of integrated double peak pharmacokinetics in this paper indicated that the three flavonoid C-glycosides may be absorbed by two sites of intestine in vivo. These results of bile flow and toe swelling showed a significant correlation between the pharmacokinetics and pharmacodynamics.. The novel integrated double peak pharmacokinetic approach to studying the holistic pharmacokinetic properties of traditional Chinese medicine has been successfully developed and validated using AM as a model drug. This study would be a useful guide for the holistic double peak pharmacokinetic study in consistence with the intrinsic theory and characteristics of traditional Chinese medicine.

Topics: Abrus; Administration, Oral; Animals; Apigenin; Carrageenan; Chromatography, High Pressure Liquid; Edema; Glucosides; Glycosides; Kidney; Liver; Lung; Male; Mass Spectrometry; Myocardium; Plant Components, Aerial; Plant Extracts; Rats, Sprague-Dawley; Spleen; Tissue Distribution