vasoactive-intestinal-peptide and thrombin-receptor-peptide-(42-55)

Recently, it has been shown that the N-terminal peptide from a new type of thrombin receptor exhibits thrombin receptor agonist activity. We examined the effects of this synthetic thrombin receptor against peptide (SFLLRNPNDKYEPF, TRAP) on human umbilical vein endothelial cells (HUVECs). TRAP induced rapid morphological changes in HUVECs, with marked increase in the release of prostacyclin, endothelin, platelet activating factor, tissue type plasminogen activator, and plasminogen activator inhibitor-1. Incubation of cells with TRAP also induced a rapid decrease in cell-surface thrombomodulin. Thus, activation of the newly described thrombin receptor may alter their role in the hemostatic pathway.

Topics: Amino Acid Sequence; Cells, Cultured; Endothelium, Vascular; Epoprostenol; Humans; Kinetics; Molecular Sequence Data; Peptide Fragments; Plasminogen Activator Inhibitor 1; Platelet Activating Factor; Receptors, Cell Surface; Receptors, Thrombin; Thrombomodulin; Tissue Plasminogen Activator; Umbilical Veins; Vasoactive Intestinal Peptide