vasoactive-intestinal-peptide and ammonium-acetate

vasoactive-intestinal-peptide has been researched along with ammonium-acetate* in 1 studies

Other Studies

1 other study(ies) available for vasoactive-intestinal-peptide and ammonium-acetate

Article	Year
High-performance liquid chromatographic determination of cyclic 3',5'-AMP with fluorescence detection. Vasoactive intestinal peptide-induced modification of its concentration in neuroblastoma cells. Journal of chromatography. B, Biomedical applications, 1994, Jul-01, Volume: 657, Issue:1 The efficiency of ion-pair reversed-phase HPLC on a Vydac C18 column with 50 mM ammonium acetate (pH 4.75)-methanol-acetonitrile (88:9:3, v/v/v) as the mobile phase with isocratic separation and fluorescence detection for the determination of cAMP in cellular extracts was evaluated. This method was compared with a radioimmunoassay technique in terms of linearity, reproducibility and sensitivity. No interactions with other nucleotides such as AMP, ADP, ATP and cGMP were observed. Application to the measurement of cAMP modifications was studied in a neuroblastoma cell line: LA-N-2 cells stimulated by a neuropeptide, vasoactive intestinal peptide. Topics: Acetates; Chromatography, High Pressure Liquid; Cyclic AMP; Humans; Hydrogen-Ion Concentration; Kinetics; Neuroblastoma; Radioimmunoassay; Sensitivity and Specificity; Tumor Cells, Cultured; Vasoactive Intestinal Peptide	1994

Article

Year

High-performance liquid chromatographic determination of cyclic 3',5'-AMP with fluorescence detection. Vasoactive intestinal peptide-induced modification of its concentration in neuroblastoma cells.

Journal of chromatography. B, Biomedical applications, 1994, Jul-01, Volume: 657, Issue:1

The efficiency of ion-pair reversed-phase HPLC on a Vydac C18 column with 50 mM ammonium acetate (pH 4.75)-methanol-acetonitrile (88:9:3, v/v/v) as the mobile phase with isocratic separation and fluorescence detection for the determination of cAMP in cellular extracts was evaluated. This method was compared with a radioimmunoassay technique in terms of linearity, reproducibility and sensitivity. No interactions with other nucleotides such as AMP, ADP, ATP and cGMP were observed. Application to the measurement of cAMP modifications was studied in a neuroblastoma cell line: LA-N-2 cells stimulated by a neuropeptide, vasoactive intestinal peptide.

Topics: Acetates; Chromatography, High Pressure Liquid; Cyclic AMP; Humans; Hydrogen-Ion Concentration; Kinetics; Neuroblastoma; Radioimmunoassay; Sensitivity and Specificity; Tumor Cells, Cultured; Vasoactive Intestinal Peptide

1994