valinomycin and 3-3--dioctadecylindocarbocyanine

The membrane potential in Entamoeba is an important driving force for the uptake of substrates. In Entamoeba invadens PZ a membrane potential of -36 mV was obtained when Nernst equation was applied to the distribution at equilibrium of 86Rb+ in the presence of valinomycin. This could explain the levels of accumulation of up to 4 times found for positively charged substrates. Membrane potential was diminished by depolarizing conditions (high external K+ concentration in the presence of valinomycin). Moreover, we recorded continuously the membrane potential of Entamoeba invadens PZ and Entamoeba histolytica HM1 using the fluorescent lipophilic cation diisopropylthiodicarbocyanine. It was found that the uptake of this cation by the amoebae was fast in both species, conditions that modify the membrane potential (hyperpolarization and depolarization) produced changes in the fluorescence of the dye in agreement with its reported capability to detect variations in membrane potential. It can be concluded that these microorganisms have a membrane potential negative inside them.

Topics: Animals; Carbocyanines; Entamoeba; Entamoeba histolytica; Membrane Potentials; Potassium; Rubidium; Valinomycin