valinomycin and 3-3--4--5-tetrachlorosalicylanilide

The non-metabolizable glucose analog, 2-deoxyglucose (2-DG), decreased the growth rate and optical density of Streptococcus bovis JB1 20%, but it had an even greater effect on stationary phase cultures. Control cultures receiving only glucose (2 mg/ml) lysed very slowly (<5% decline in optical density in 48 h), but cultures that had been grown with glucose and 2-DG (2 mg/ml each) lysed much faster (>85% decline in optical density in 48 h). Cultures that were treated with inhibitors that decreased intracellular ATP (sodium fluoride, nigericin, and valinomycin or tetrachlorosalicylanilide) or membrane potential (sodium fluoride, nigericin, and valinomycin, tetrachlorosalicylanilide, or phenylmethylsulfonyl fluoride) did not promote lysis. 2-DG had its greatest effect when it was added at inoculation. If 2-DG was added at later times, less lysis was observed, and cells that were given 2-DG just prior to stationary phase were unaffected. Cells that were grown with glucose and 2-DG were more susceptible to cell wall-degrading enzymes (lysozyme and mutanolysin) than cells that had been grown only with glucose, but sublethal doses of penicillin during growth did not promote lysis after the cells had reached stationary phase. The idea that 2-DG might be affecting autolytic activity was supported by the observation that cultures washed and resuspended in fresh medium with or without 2-DG lysed at a slower rate than cultures that were not centrifuged or were resuspended in the culture supernatant.

Topics: Adenosine Triphosphate; Anti-Bacterial Agents; Antimetabolites; Bacteriolysis; Cell Wall; Deoxyglucose; Endopeptidases; Enzyme Inhibitors; Glucose; Membrane Potentials; Muramidase; Nigericin; Penicillin G; Penicillins; Protease Inhibitors; S Phase; Salicylanilides; Sodium Fluoride; Streptococcus bovis; Tosyl Compounds; Valinomycin

The mechanism by which K+ inhibits vanadate-induced 45Ca2+ influx by human red blood cells (RBC) was studied using several independent approaches. The following results were found: 1. The inhibitory effect of K+ was absent when RBC were loaded with a Ca(2+)-chelator. This treatment at the same time inhibited the vanadate-induced K+ efflux, and the membrane hyperpolarization induced by Ca2+ in vanadate-treated cells. 2. The potency of K+, Rb+, and Cs+ to inhibit vanadate-induced Ca2+ influx corresponded to their ability to depolarize the RBC membrane via the Ca(2+)-activated K+ channel (K(Ca)). 3. Inhibition of the vanadate-induced 45Ca2+ influx by a protonophore proceeded in parallel with the inhibition of the vanadate-plus-Ca(2+)-induced membrane hyperpolarization. 4. Valinomycin in part released the inhibition of the vanadate-induced Ca2+ influx by known K(Ca) inhibitors (quinine, oligomycin, 4-aminopyridine) but not by inhibitors of the Ca2+ channel (Cu2+, HS-reagents, organic Ca2+ channel blockers). 5. K+ did not inhibit the vanadate-induced Ca2+ influx in dog RBC which have K(Ca) but no transmembrane K+ gradient. The inhibition of the vanadate-induced Ca2+ influx by external K+ appears to be due to the elimination of the electrical component of the Ca(2+)-motive force imposed by opening of the K(Ca). This implies that the Ca2+ carrier mediating the influx of Ca2+ in the presence of vanadate is of uniport type, and that the activity of K(Ca) may serve as a supporting element for Ca2+ influx.

Topics: Aminoquinolines; Animals; Calcium; Chelating Agents; Dogs; Egtazic Acid; Erythrocytes; Female; Humans; In Vitro Techniques; Ion Transport; Male; Membrane Potentials; Potassium; Potassium Channels; Salicylanilides; Uncoupling Agents; Valinomycin; Vanadates

Bacillus licheniformis NCIB 6346 showed active accumulation of glucose which was inhibited by agents which affect the transmembrane proton gradient. Phosphotransferase (PTS) activity, identified as phosphoenolpyruvate-dependent phosphorylation of glucose, was found in cell extracts but could not be demonstrated in cells permeabilized with toluene when assays were conducted at pH 6.6. The same was true for mannitol and fructose phosphotransferase activities. Cells grown on fructose accumulated glucose at a slower rate than glucose-grown cells, and extracts prepared from them did not contain glucose PTS activity. Examination of the effects of analogs on glucose uptake and phosphorylation showed that 2-deoxyglucose was not a PTS substrate, but did markedly inhibit glucose uptake, with stronger inhibition in cells grown on fructose. Glucose accumulation by whole cells grown on glucose became less sensitive to the uncoupler tetrachlorosalicylanilide (TCS) as the pH was raised from 6.6 to 8.0, while in fructose-grown cells TCS was equally effective across this pH range. PTS activity was exhibited by toluene-treated cells at pH 7.5 and above, although the system itself in extracts was not affected by pH in the range of 5.0 to 8.0. The results are consistent with the presence of two glucose transport systems, one a PTS and the other operating by an alternative mechanisms, and suggest that the PTS in B. licheniformis may be regulated in a pH-dependent manner.

Topics: Bacillus; Biological Transport, Active; Cell Membrane Permeability; Energy Metabolism; Glucose; Hydrogen-Ion Concentration; Monosaccharide Transport Proteins; Onium Compounds; Organophosphorus Compounds; Phosphorylation; Phosphotransferases; Proton-Translocating ATPases; Salicylanilides; Sugar Phosphates; Valinomycin

Pyranine was incorporated into sonicated unilamellar vesicles of soybean phosphatidylcholine to monitor changes in the internal pH of the vesicles. Dilution of soybean phosphatidylcholine vesicles loaded with 0.3 M KCl, KNO3 or K2SO4 into salt-free buffer resulted in rapid exchange of K+ and protons. A pseudoequilibrium distribution of ions was achieved, since addition of valinomycin, uncoupler or nigericin now caused a rapid alkalinization of the vesicle interior. Dilution into buffer containing NaCl gave a further exchange of Na+ and protons following the initial K+/proton exchange. Na+ permeation was slower than that of K+. A stable membrane potential was not generated by the ion movements. It is proposed that aqueous channels are formed through the phospholipid bilayers and that K+ and Na+ permeate through these channels as the hydrated ions.

Topics: Arylsulfonates; Cations; Coloring Agents; Glycine max; Hydrogen-Ion Concentration; Ion Channels; Liposomes; Membrane Potentials; Nigericin; Nitrates; Particle Size; Phosphatidylcholines; Potassium; Potassium Chloride; Potassium Compounds; Protons; Salicylanilides; Sodium; Sulfates; Valinomycin

A fluorescence-quench method using acridine orange as the probe was employed to monitor acid formation in situ by detergent-permeabilized gastric glands. In KCl medium, the addition of ATP to the permeabilized glands resulted in a rapid decrease in fluorescence and addition of valinomycin resulted in a second phase of fluorescence quench. The fluorescence was restored by addition of the H+-K+-ATPase inhibitor, Sch 28080. An ATP-dependent fluorescence quench was observed also in K2SO4 or K+-isethionate medium; however, valinomycin was ineffective in the Cl-free media. The ATP-dependent quench could be reversed or prevented by the electrogenic protonophore, tetrachlorosalicylanilide (TCS), in KCl medium but not in Cl-free media. The results with TCS are interpreted as demonstrating a large Cl- conductance in the secretory membrane, whereas the results with valinomycin indicate that resting membranes lack a K+ conductance. The data suggest that a complex KCl pathway that may demonstrate a Cl- conductance is used to activate acid secretion.

Topics: Animals; Anti-Ulcer Agents; Cell Membrane; Chloride Channels; Chlorides; Gastric Mucosa; Imidazoles; In Vitro Techniques; Ion Channels; Kinetics; Membrane Proteins; Models, Theoretical; Parietal Cells, Gastric; Rabbits; Salicylanilides; Spectrometry, Fluorescence; Valinomycin

Protonophores have been used frequently to determine changes in membrane potential in suspensions of red cells, since such changes are reflected by changes in extracellular pH, due to proton and consequently protonophore reequilibration. In a previous paper (Bennekou, P. 1988, J. Membrane Biol. 102:225-234) a kinetic model for the translocation of a protonophore, CCCP, across the human red cell membrane was established. This model accounts for the protonophore reequilibration following abrupt changes in membrane potential. In this paper the limitations of the method with regard to the estimation of transient membrane potentials are examined, using the transport model to simulate changes in extracellular pH in response to noninstantaneous changes in membrane potential. The temperature and time resolution calculated from the model are reported. Furthermore, it is shown that the transport model established for CCCP is valid for another protonophore, TCS, thus indicating the general validity of the transport scheme for the entire class of protonophores.

Topics: Carbonyl Cyanide m-Chlorophenyl Hydrazone; Cell Membrane Permeability; Erythrocyte Membrane; Humans; Hydrogen-Ion Concentration; Membrane Potentials; Methods; Nitriles; Potassium; Salicylanilides; Temperature; Valinomycin

The addition of glucose to a suspension of Ehrlich ascites tumor cells results in rapid acidification of the extracellular medium due to lactic acid production. The nature of the H+ efflux mechanism has been studied by measuring the time course of the acidification, the rate of proton efflux, the direction and relative magnitude of the H+ concentration gradient, and the voltage across the membrane. Using the pH-sensitive dye acridine orange, we have established that after addition of 10 mM glucose an outward-directed H+ concentration gradient develops. As the rate of glycolysis slows, the continued extrusion of H+ reverses the direction of the H+ concentration gradient. Changes in absorbance of the voltage-sensitive dye diethyloxadicarbocyanine iodide (DOCC), and changes in the distribution of the lipid permeant cation tetraphenyl phosphonium, showed a dramatic and persistent hyperpolarization of the membrane voltage after glucose addition. The hyperpolarization was prevented by the protonophore tetrachlorosalicylanalide (TCS) and by valinomycin, but not by the neutral-exchange ionophore nigericin. Inhibitors of lactate efflux were found to reduce the rate of acidification after glucose addition but they had no effect on the magnitude of the resulting hyperpolarization. On the basis of these and other data we suggest that an active electrogenic pump mechanism for H+ efflux may be activated by glucose and that this mechanism operates independently of the lactate carrier system.

Topics: Acridine Orange; Animals; Carbocyanines; Carcinoma, Ehrlich Tumor; Cell Membrane; Coloring Agents; Glucose; Glycolysis; Hydrogen-Ion Concentration; Kinetics; Mice; Onium Compounds; Organophosphorus Compounds; Protons; Salicylanilides; Valinomycin