valencene and germacra-1(10)-4-11(13)-trien-12-oic-acid

valencene has been researched along with germacra-1(10)-4-11(13)-trien-12-oic-acid* in 1 studies

Other Studies

1 other study(ies) available for valencene and germacra-1(10)-4-11(13)-trien-12-oic-acid

Article	Year
A chicory cytochrome P450 mono-oxygenase CYP71AV8 for the oxidation of (+)-valencene. FEBS letters, 2011, Jan-03, Volume: 585, Issue:1 Chicory (Cichorium intybus L.), which is known to have a variety of terpene-hydroxylating activities, was screened for a P450 mono-oxygenase to convert (+)-valencene to (+)-nootkatone. A novel P450 cDNA was identified in a chicory root EST library. Co-expression of the enzyme with a valencene synthase in yeast, led to formation of trans-nootkatol, cis-nootkatol and (+)-nootkatone. The novel enzyme was also found to catalyse a three step conversion of germacrene A to germacra-1(10),4,11(13)-trien-12-oic acid, indicating its involvement in chicory sesquiterpene lactone biosynthesis. Likewise, amorpha-4,11-diene was converted to artemisinic acid. Surprisingly, the chicory P450 has a different regio-specificity on (+)-valencene compared to germacrene A and amorpha-4,11-diene. Topics: Biocatalysis; Cichorium intybus; Cloning, Molecular; Cytochrome P-450 Enzyme System; DNA, Complementary; Expressed Sequence Tags; Gene Library; Molecular Sequence Data; Oxidation-Reduction; Oxidoreductases; Plant Proteins; Polycyclic Sesquiterpenes; Sequence Analysis, DNA; Sesquiterpenes; Sesquiterpenes, Germacrane; Stereoisomerism; Yeasts	2011

Article

Year

A chicory cytochrome P450 mono-oxygenase CYP71AV8 for the oxidation of (+)-valencene.

FEBS letters, 2011, Jan-03, Volume: 585, Issue:1

Chicory (Cichorium intybus L.), which is known to have a variety of terpene-hydroxylating activities, was screened for a P450 mono-oxygenase to convert (+)-valencene to (+)-nootkatone. A novel P450 cDNA was identified in a chicory root EST library. Co-expression of the enzyme with a valencene synthase in yeast, led to formation of trans-nootkatol, cis-nootkatol and (+)-nootkatone. The novel enzyme was also found to catalyse a three step conversion of germacrene A to germacra-1(10),4,11(13)-trien-12-oic acid, indicating its involvement in chicory sesquiterpene lactone biosynthesis. Likewise, amorpha-4,11-diene was converted to artemisinic acid. Surprisingly, the chicory P450 has a different regio-specificity on (+)-valencene compared to germacrene A and amorpha-4,11-diene.

Topics: Biocatalysis; Cichorium intybus; Cloning, Molecular; Cytochrome P-450 Enzyme System; DNA, Complementary; Expressed Sequence Tags; Gene Library; Molecular Sequence Data; Oxidation-Reduction; Oxidoreductases; Plant Proteins; Polycyclic Sesquiterpenes; Sequence Analysis, DNA; Sesquiterpenes; Sesquiterpenes, Germacrane; Stereoisomerism; Yeasts

2011