urea has been researched along with tryptophan in 462 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 223 (48.27) | 18.7374 |
| 1990's | 76 (16.45) | 18.2507 |
| 2000's | 112 (24.24) | 29.6817 |
| 2010's | 46 (9.96) | 24.3611 |
| 2020's | 5 (1.08) | 2.80 |
| Authors | Studies |
|---|---|
| Barnes, JC; Bradley, P; Day, NC; Fourches, D; Reed, JZ; Tropsha, A | 1 |
| Kuschinsky, G | 1 |
| Jaton, JC; Riesen, WF; Schlessinger, J | 1 |
| Goyffon, M; Toulmé, JJ; Villa, F | 1 |
| Tsong, TY | 2 |
| Daurat-Larroque, ST; Portuguez, ME; Santomé, JA | 1 |
| Irie, M; Ohgi, K | 1 |
| Léonis, J; Looze, Y; Polastro, E | 1 |
| Lischwe, MA; Sung, MT | 1 |
| Miller, JN; Nwokedi, GI | 1 |
| Cocchi, D; Manneschi, M; Müller, EE; Panerai, AE; Salerno, F | 1 |
| Fokkens, W; Haije, WG; Hop, WC; Peters, HA; Portengen, H; Teulings, FA; van der Werf-Messing, B | 1 |
| Cole, DJ; Lewis, D; Sohail, MA | 1 |
| Irie, M; Yano, Y | 1 |
| Abramovitz, R; Elson, D; Schechter, N; Spitnik-Elson, P | 1 |
| Bell, JE; Castellino, FJ; Hill, RL; Trayer, IP | 1 |
| Brand, JG; Cagan, RH | 1 |
| Komiyama, T; Miwa, M; Murao, S; Sato, S | 1 |
| Cunningham, PJ; Lewis, AJ; Moser, BD; Peo, ER | 2 |
| Oratz, M; Rothschild, MA; Schreiber, SS | 3 |
| Elson, D; Schechter, N; Spitnik-Elson, P | 1 |
| Ashida, K; Yokogoshi, H | 1 |
| Salhuddin, A; Waheed, A | 1 |
| Fuller, MF; Livingstone, RM; Mennie, I | 1 |
| Dailey, HA; Proulx, KL | 1 |
| Martorana, GE; Meucci, E; Miggiano, GA; Mordente, A; Santini, SA | 1 |
| Goñi, FM; Rial, E; Viguera, AR | 1 |
| Erickson, RE; Laurents, DV; Pace, CN | 1 |
| Banik, U; Bhattacharyya, B; Mandal, NC; Roy, S; Saha, R | 1 |
| Berman, HA; Nowak, MW | 1 |
| Dostmann, WR; León, DA; Taylor, SS | 1 |
| Bujalowski, W; Lohman, TM | 1 |
| Drake, SK; Frerman, FE; Loehr, JP; Watmough, NJ | 1 |
| Bârzu, O; Danchin, A; Gilles, AM; Glaser, P; Krin, E; Munier, H; Pellecuer, C; Rose, T | 1 |
| Mathur, RL; Sahai, P | 1 |
| Criscimagna, NL; Horowitz, PM | 1 |
| Florio, C; Puppini, P; Traversa, U; Vertua, R | 1 |
| Bras, GL; Deville-Bonne, D; Garel, JR; Teschner, W | 1 |
| Addetia, R; Livesey, DL; Mailer, K | 1 |
| Beale, D | 1 |
| Berger, JW; Vanderkooi, JM | 1 |
| Chock, PB; Federici, MM; Stadtman, ER | 1 |
| Hoenders, HJ; van den Oetelaar, PJ | 1 |
| Gonnelli, M; Strambini, GB | 3 |
| Chaminade, F; Muriel, MP; Stanescu, V | 1 |
| Cramers, CA; Dijkstra, JB; Ringoir, SM; Schoots, AC; Vanholder, R | 1 |
| Jardetzky, O; Lane, AN | 1 |
| Dix, JA; Lukacovic, MF; Tinklepaugh, MS; Verkman, AS | 1 |
| Homandberg, G; Lai, CS; Miziorko, H; Wolff, C | 1 |
| Agarwal, SK; Hangloo, S; Khan, MY | 1 |
| Heintges, MG; Savory, J; Sobel, RE | 1 |
| Griffin, EE; Miller, LL | 1 |
| Snell, EE; Wada, H | 1 |
| Eylar, EH | 1 |
| Dunlap, RB; Liu, JK | 1 |
| Metz, H; Preac-Mursic, V | 1 |
| Kay, CM; Murray, AC; Oikawa, K | 1 |
| Guire, P | 1 |
| Schellenberg, KA | 1 |
| Penzer, GR; Radda, GK | 1 |
| Diederich, DA; Grisolia, S | 1 |
| Veeger, C; Visser, J | 1 |
| De Flora, A; Horecker, BL; Melloni, E; Pontremoli, S | 2 |
| Frith, LO; Kirsch, RE; Saunders, SJ; Stead, RH | 1 |
| Higashino, K; Kudo, S; Yamamura, Y | 1 |
| Masana, Y; Nakagawa, I; Suzuki, T; Takahashi, T | 1 |
| Fellenberg, G | 1 |
| Brown, EM; Parry, RM | 1 |
| Rao, KR; Vyas, GN | 2 |
| Asafov, GB; Ekisenina, NI; Ivanova, LM; Lumel'skiĭ, VIa; Smagin, VG | 1 |
| Chen, NC; Gholson, RK | 1 |
| Neale, S | 1 |
| Holmgren, A | 1 |
| Gerard, D; Lami, H; Laustriat, G | 1 |
| Jacobs, HK; Kuby, SA; Maland, L; Palmieri, R; Wu, L; Yue, RH | 1 |
| King, TP | 1 |
| Comty, CM; Shapiro, F; Wathen, RL | 1 |
| Chen, RF | 1 |
| Crawford, IP; Kida, S | 1 |
| Eidus, LKh; Kalabukhova, TN; Kondakova, NV | 1 |
| Parrott, CL; Shifrin, S | 1 |
| Kerwar, G; Lehrer, SS | 1 |
| Bergström, J; Bucht, H; Fürst, P; Hultman, E; Josephson, B; Norée, LO; Vinnars, E | 1 |
| Tritsch, GL | 1 |
| Lindskog, S; Nilsson, A | 1 |
| Corrigall, A; Frith, L; Kelman, L; Kirsch, RE; Saunders, SJ; Terblanche, J; Wicht, S | 1 |
| Gaffin, SL; Watanabe, S | 1 |
| Honikel, KO; Madsen, NB | 1 |
| Cohen, HP; Lin, S | 1 |
| Wells, MA | 2 |
| Hopkins, TR; Spikes, JD | 2 |
| Morikawa, H | 1 |
| Arrio, B; Hill, M; Parquet, C | 1 |
| Daniel, LJ; Gray, LF | 1 |
| Andrews, LJ; MacKNIGHT, ML; Ryan, J; Tollin, G | 1 |
| Brown, JA; Cline, TR | 1 |
| Callahan, HJ; Liberti, PA; Maurer, PH | 1 |
| Herskovits, TT; Sorensen, M | 2 |
| Crawford, IP; Hathaway, GM; Kida, S | 1 |
| Donovan, JW | 1 |
| Jackson, DA; Yanofsky, C | 1 |
| Ananthanarayanan, VS; Bigelow, CC | 2 |
| Janecek, J; Spízek, J | 1 |
| Aloj, SM; Blasi, F; Goldberger, RF | 1 |
| Nicholson, BH | 1 |
| Grochowski, BJ; Luborsky, SW; Shifrin, S | 1 |
| Campbell, TG; Catsimpoolas, N; Meyer, EW | 1 |
| Calissano, P; Friesen, A; Moore, BW | 1 |
| Buchowicz, I; Goch, H; Zakrzewski, K | 1 |
| Angeletti, RH | 1 |
| Dolif, D; Jürgens, P | 1 |
| Elödi, P; Krysteva, M | 1 |
| Crawford, IP; Maurer, R | 1 |
| Dannies, PS; Levine, L | 1 |
| DeMoss, RD; Hoch, SO | 1 |
| Epand, RM | 1 |
| Mori, KF | 1 |
| Gros, C; Labouesse, B; Lemaire, G; Van Rapenbusch, R | 2 |
| Chicheportiche, R; Lazdunski, M; Rochat, C; Sampieri, F | 1 |
| Fox, HM; Kies, C | 2 |
| Huntley, TE; Strittmatter, P | 1 |
| Aloj, S; Edelhoch, H | 2 |
| Harris, C; Metzler, DE; Siano, D; Thomson, JA; Yang, IY | 1 |
| Azhir, A; Chignell, DA; Gratzer, WB | 1 |
| Kay, CM; McCubbin, WD; Oikawa, K | 1 |
| Lehrer, SS | 1 |
| Buchwald, BM | 1 |
| Fels, IG | 1 |
| Kasarda, DD; Platt, SG | 1 |
| Chiba, H; Hirose, M; Sugimoto, E | 1 |
| Fishman, WH; Lin, CW; Sie, HG | 1 |
| Gabel, D; Katchalski, E; Steinberg, IZ | 1 |
| Herskovits, TT; Villanueva, GB | 2 |
| Schwarz, E | 1 |
| Cowgill, RW; Jones, DC | 1 |
| Timpl, R; Weiser, M; Wolff, I | 1 |
| Cowey, CB; Knox, D; Lush, IE | 1 |
| Klotz, IM; Royer, GP; Sloniewsky, AR | 1 |
| McKay, RH | 1 |
| McLean, GW; Schellenberg, KA | 1 |
| Gordon, WG; Groves, ML | 1 |
| Ando, T; Kawashima, S | 1 |
| Fonss-Bech, P; Schmidt, KD | 1 |
| Brewer, JM; Fairwell, T; Lovins, RE; Travis, J | 1 |
| Bultitude, FW | 1 |
| Pongs, O | 1 |
| Alfsen, A; Cittanova, N | 1 |
| Takahashi, K | 1 |
| Kornegay, ET; Mosanghini, V; Snee, RD | 1 |
| Elödi, P; Libor, S | 2 |
| Hatfield, EE; Kaminski, J; Nimrick, K; Owens, FN | 1 |
| Bocchini, V | 1 |
| Sinn, I; Stähler, F | 1 |
| DeSena, T; Herskovits, TT; Jaillet, H | 1 |
| Andersson, LO; Gejvall, T; Rosén, CG | 1 |
| Edelhoch, H; Lippoldt, RE | 1 |
| Berg, T; Catsimpoolas, N; Wang, J | 1 |
| Azuma, T; Hamaguchi, K; Migita, S | 1 |
| Kerly, M; Spruyt, JE | 1 |
| Kasper, CB; Khandwala, AS | 1 |
| Sander, C; Ts'o, PO | 1 |
| Mongelli, J; Oratz, M; Rothschild, MA; Schreiber, SS | 1 |
| Cohen, S; Thrasher, SG | 1 |
| Fürst, P; Guarnieri, G; Hultman, E | 1 |
| Brown, HH | 1 |
| Cowgill, RW | 1 |
| Bailey, JE; Beaven, GH; Chignell, DA; Gratzer, WB | 1 |
| Attallah, NA; Lata, GF | 1 |
| Mihalyi, E | 1 |
| DeMoss, JA; Gaertner, FH | 1 |
| Coon, MJ; Cottam, GL; Hollenberg, PF | 1 |
| Oravec, M; Sourkes, TL | 1 |
| Dobryszycka, W; Kukral, JC; Pusztai, A | 1 |
| Herskovits, TT | 1 |
| Greenblatt, J; Herskovits, TT | 1 |
| Farber, EM; Zackheim, HS | 1 |
| Fishman, L; Mongelli, J; Oratz, M; Rothschild, MA; Schreiber, SS | 1 |
| Preddie, EC | 1 |
| Augenstein, L; Burke, M | 1 |
| Dyson, JE; Noltmann, EA | 1 |
| Funatsu, M; Hamaguchi, K; Hayashi, K; Imoto, T; Takahashi, T | 1 |
| Burger, HG; Edelhoch, H | 1 |
| Krasner, J; McMenamy, RH | 1 |
| Burger, HG; Condliffe, PG; Edelhoch, H; Lippoldt, RE | 1 |
| Steiner, RF | 2 |
| Dolan, G; Godin, C | 1 |
| Griffin, D; Nelson, B; Rosenberg, LT; Tachibana, DK | 1 |
| Bhatnagar, GM; Martin, CJ | 1 |
| Baudras, A; Di Franco, A; Iwatsubo, M; Labeyrie, F | 1 |
| Kronman, MJ | 1 |
| Kasuya, M; Takashina, H | 1 |
| Masana, Y; Nakagawa, I | 1 |
| Kamenich, W; Leimenstoll, A; Leimenstoll, G; Matthes, KJ; Moch, KJ | 1 |
| Chasan, B; Dix, JA; Lukacovic, MF; Solomon, AK; Toon, MR; Verkman, AS | 1 |
| Armenian, AG; Gasparian, VK; Mardanian, SS; Nalbandian, RM | 1 |
| Boteva, R; Filippi, B; Genov, N; Jori, G; Ricchelli, F; Shopova, M | 1 |
| Komiyama, T; Miwa, M | 1 |
| Burstein, EA; Gerday, C; Permyakov, EA; Yarmolenko, VV | 1 |
| Atreyi, M; Rajeswari, MR; Rao, MV | 1 |
| Cariello, L; Jori, G; Salvato, B | 1 |
| Villanueva, GB | 1 |
| Kwiram, AL; Ross, JB; Rousslang, KW | 1 |
| Filippi, B; Jori, G; Ricchelli, F; Salvato, B | 1 |
| Mooney, D; Simpkins, H; Thompson, LM | 1 |
| Choi, SG; Hardman, JK | 1 |
| Choi, SG; Hardman, JK; O'Donnell, SE; Sarken, KD | 1 |
| Calsamiglia, S; Firkins, JL; Stern, MD | 1 |
| Brandon, MR; Cardamone, M; Puri, NK | 1 |
| Brouillette, CG; Chaiken, IM; Myszka, DG; Sweet, RW; Tendian, SW | 1 |
| McClelland, DA; Price, NC; Stevens, L | 1 |
| Desmadril, M; Garcia, P; Minard, P; Yon, JM | 1 |
| Lapanje, S; Poklar, N; Vesnaver, G | 1 |
| Bhattacharyya, B; Sackett, DL; Wolff, J | 1 |
| Thumser, AE; Wilton, DC | 1 |
| Brown, PR; Marsh, P; Tata, R | 1 |
| Frieden, C; Hoeltzli, SD | 1 |
| Baracca, A; Parenti Castelli, G; Solaini, G; Strambini, GB | 1 |
| Fersht, AR; Loewenthal, R; Sancho, J; Vuilleumier, S | 1 |
| Augusteyn, RC; Ghiggino, KP; Putilina, T | 1 |
| Atkinson, T; Badcoe, IG; Burston, SG; Clarke, AR; Holbrook, JJ; Jackson, GS; Smith, CJ; Staniforth, RA | 1 |
| Fee, L; Gray, T; Grimsley, G; Pace, CN; Vajdos, F | 1 |
| Gougeon, R; Marliss, EB; Pencharz, PB | 1 |
| Easter, RA; Kerr, BJ | 1 |
| Prinsen, CF; Veerkamp, JH | 1 |
| Agarwalla, S; Balaram, P; Gokhale, RS; Santi, DV | 1 |
| Clark, PL; Gierasch, LM; Liu, ZP; Zhang, J | 1 |
| Arunachalam, U; Kellis, JT | 1 |
| Brinkmann, U; Jung, SH; Lee, B; Pastan, I; Reiter, Y | 1 |
| Adeola, O | 1 |
| Mas, MT; Szpikowska, BK | 1 |
| Mahoney, S; Martin, KS; Reedstrom, RJ; Royer, CA; Vangala, S; Wilker, EW | 1 |
| Bowler, BE; Dong, A; Garbin, K; Godbole, S | 1 |
| Hamilton, CR; Libal, GW; Uttecht, DJ | 1 |
| Brandt, RT; Campbell, CG; Cochran, RC; Nagaraja, TG; Titgemeyer, EC | 1 |
| Altman, S; Fersht, AR; Golbik, R; Gopalan, V; Schreiber, G | 1 |
| Gloss, LM; Matthews, CR | 1 |
| Ghosh, S; Pattnaik, BR; Rajeswari, MR | 1 |
| MacDonald, RI; Pantazatos, DP | 1 |
| Hirose, M; Onda, M; Takahashi, N; Tatsumi, E | 1 |
| St Jean, G; Titgemeyer, EC; Wessels, RH | 1 |
| Don, Y; Li, H; Liu, N; Wu, H; Yang, K; Zhang, Y | 1 |
| Lehrer, SS; Mabuchi, K; Maéda, Y; Zhou, X | 1 |
| Devi, NA; Rao, AG | 1 |
| Linetsky, M; Ortwerth, BJ; Ranson, N | 1 |
| Kosk-Kosicka, D; Lopez, MM | 1 |
| de Prat-Gay, G; Foguel, D; Silva, JL | 1 |
| Doniach, S; Fink, AL; Karnoup, AS; Segel, DJ; Seshadri, S; Uversky, VN | 1 |
| Burton, RE; Ghaemmaghami, S; Oas, TG; Richardson, JS; Word, JM | 1 |
| Busti, P; Delorenzi, NJ; Gatti, CA | 1 |
| Komath, SS; Padma, P; Swamy, MJ | 1 |
| Das, BK; Liang, JJ | 1 |
| Balaram, H; Balaram, P; Gokhale, RS; Ray, SS | 1 |
| Baskakov, IV; Bolen, DW | 1 |
| Hisatomi, O; Matsuda, S; Tokunaga, F | 1 |
| Kobayashi, K; Kohno, T; Satoh, A; Tsuji, T; Yanagawa, H; Yoshida, K | 1 |
| Atkins, WM; Daggett, V; Storch, EM | 1 |
| Biktashev, AG; Khaitlina, SY; Kuznetsova, IM; Turoverov, KK | 1 |
| DiNardo, AA; Merrill, AR; Steer, BA | 1 |
| Glockshuber, R; Liemann, S; Wildegger, G | 1 |
| Abdullaev, ZK; Arseniev, AS; Bocharov, EV; Dolgikh, DA; Kirpichnikov, MP; Latypov, RF; Melnik, TN; Uversky, VN; Vassilenko, KS | 1 |
| Sui, SF; Wu, Y | 1 |
| Grips, E; Holm, E; Sedlaczek, O | 1 |
| Balny, C; Heremans, K; Lange, R; Meersman, F; Ruan, K | 1 |
| Colombo, R; DalleDonne, I; Milzani, A | 1 |
| Bortoleto, RK; Ward, RJ | 1 |
| Lücke, C; Rademacher, M; Rüterjans, H; Veerkamp, JH; Zimmerman, AW | 1 |
| Behere, DV; Deva, MS | 1 |
| Broadhead, RL; Hart, CA; Manary, MJ; Yarasheski, KE | 1 |
| Bigotti, MG; Brunori, M; Cutruzzolà, F; Giannini, S; Staniforth, RA; Travaglini-Allocatelli, C | 1 |
| Canaves, JM; Leon, DA; Taylor, SS | 1 |
| Kleinelanghorst, LB; Knieps, EA; Kula, MR; Rämsch, C; Thömmes, J | 1 |
| Burke, J; Dirr, HW; Wallace, LA | 1 |
| Berggård, T; Linse, S; Silow, M; Thulin, E | 1 |
| Eastman, P; Fink, AL; Nishimura, C; Riley, R | 1 |
| Favier-Perron, B; Hofmann, A; Huber, R; Lewit-Bentley, A; Mesonero, J; Raguénès-Nicol, C; Russo-Marie, F | 1 |
| Iwakura, M; Maki, K; Nakamura, T; Yamane, C | 1 |
| Aspée, A; Lissi, EA | 1 |
| Bhuyan, AK; Juneja, J; Krishnamoorthy, G; Sridevi, K; Udgaonkar, JB | 1 |
| Mushahid Khan, M; Sharma, N; Tayyab, S | 1 |
| Chaudhuri, AR; Hausheer, FH; Ludueña, RF; Schwarz, PM; Seetharamalu, P | 1 |
| Choe, SE; Li, L; Matsudaira, PT; Shakhnovich, EI; Wagner, G | 1 |
| Foguel, D; Gaspar, LP; Pinheiro, AS; Rebello, MA; Silva, JL; Terezan, AF | 1 |
| Bandyopadhyay, AK; Krishnamoorthy, G; Sonawat, HM | 1 |
| Delgado, C; Gavilanes, F; Gómez-Gutiérrez, J; Núñez, E; Pacheco, B; Peterson, DL; Yélamos, B | 1 |
| Ropson, IJ; Rousseau, DL; Yeh, SR | 1 |
| MacDonald, RI; Pozharski, EV | 1 |
| Ahmad, A; Akhtar, MS; Bhakuni, V | 1 |
| Xie, ZQ; Xu, GJ; Yuan, C; Zhang, FW | 1 |
| Bigotti, MG; Brunori, M; Cutruzzolà, F; Gianni, S; Travaglini-Allocatelli, C | 1 |
| Bai, P; Chakraborty, S; Haas, E; Ittah, V; Luo, L; Peng , Z | 1 |
| Brown, P; Karmali, A; Pacheco, R; Tata, R | 1 |
| Chaudhuri, AR; Khan, IA; Ludueña, RF | 1 |
| Gekko, K; Ohmae, E; Sasaki, Y | 1 |
| Hammarström, P; Jiang, X; Kelly, JW; Petrassi, HM; Sacchettini, JC; Smith, CS; White, JT | 1 |
| Jensen, ON; Mann, M; Petersen, J; Steen, H | 1 |
| Matthews, KS; Nichols, NM | 1 |
| Bottomley, SP; Tew, DJ | 1 |
| Finazzi-Agrò, A; Maccarrone, M; Malvezzi-Campeggi, F; Rosato, N | 1 |
| Bann, JG; Frieden, C; Hultgren, SJ; Pinkner, J | 1 |
| Breslin, PA; Keast, RS | 1 |
| Buczko, W; Mysliwiec, P; Pawlak, D; Tankiewicz, A | 1 |
| Cheng, Y; Wang, J; Wang, Z; Zhao, J | 1 |
| Kasturi, SR; Kuchroo, K; Maity, H | 1 |
| Fresnedo, O; Martínez, R; Ochoa, B; Romero, JR | 1 |
| Bandyopadhyay, S; Bose, S; Deb, S; Roy, S | 1 |
| Kumar, A; Kumar, S; Sarma Dagger, PV; Sharma Double Dagger, AK | 1 |
| Adams, PS; Luck, LA; Salopek-Sondi, B; Swartz, D | 1 |
| Pradeep, L; Udgaonkar, JB | 1 |
| Mossing, MC; Satumba, WJ | 1 |
| Sridevi, K; Udgaonkar, JB | 1 |
| Balaram, H; Balaram, P; Maithal, K; Pattanaik, P; Ravindra, G; Sengupta, C | 1 |
| Singh, S; Zlotnick, A | 1 |
| Chang, GG; Chang, HC | 1 |
| Chakrabartty, A; Cox, JA; Durussel, I; Gombos, Z; Ikura, M; Rose, DR | 1 |
| Horii, T; Sekiguchi, M; Takahashi, M; Yamazaki, J | 1 |
| Bournazel, MM; Breslin, PA; Keast, RS | 1 |
| Ha, SC; Kim, DR; Kim, KK; Lee, I | 1 |
| Banerjee, KK; Chattopadhyay, K | 1 |
| Federici, L; Fersht, AR; Freund, SM; Lovell, SC; Luisi, BF; Mayor, U; Stollar, EJ | 1 |
| Bateman, OA; Kappé, G; Lubsen, NH; Sarra, R; Slingsby, C; van Genesen, ST | 1 |
| Anderson, E; Braswell, EH; Doyle, SM; Teschke, CM; Zhu, D | 1 |
| HRADILOVA, L; PECHAR, J; PLACER, Z | 1 |
| Bird, HR; Featherston, WR; Harper, AE | 1 |
| NOZAKI, Y; TANFORD, C | 1 |
| FUNATSU, M; HAYASHI, K; IMOTO, T | 1 |
| DURKEE, AB; SIROIS, JC | 1 |
| LESTRADET, H | 1 |
| HARRINGTON, H; SAKAMI, W | 1 |
| CAMA, HR; RAO, GJ | 1 |
| HARALAMBIE, G | 1 |
| EDELHOCH, H; FRATTALI, V; LIPPOLDT, RE; STEINER, RF | 1 |
| DIMLER, RJ; WU, YV | 1 |
| EDELHOCH, H; FRATTALI, V; STEINER, RF | 1 |
| CHIBATA, I; SANO, R; TOSA, T | 1 |
| HAMOIR, G; KONOSU, S; PECHERE, JF | 1 |
| SENFT, AW | 1 |
| Taylor, SS; Zawadzki, KM | 1 |
| Antalík, M; Sedlák, E; Sprinzl, M; Sut'ák, R; Zoldák, G | 1 |
| Acharya, N; Saikrishnan, K; Talawar, RK; Varshney, U; Vijayan, M | 1 |
| de Oliveira, AC; Lima, SM; Peabody, DS; Silva, JL | 1 |
| Aoki, T; Igarashi, K; Kawano, K; Matsubara, K; Matsuura, A; Mizuguchi, M; Nagadome, H; Shinohara, Y; Takeuchi, M; Terada, Y | 1 |
| Bigotti, MG; Brunori, M; Cutruzzolà, F; Musto, R; Travaglini-Allocatelli, C | 1 |
| Frieden, C; Shu, Q | 1 |
| Fang, H; Wang, G; Xia, X | 1 |
| Holm, J; Ipsen, H; Mogensen, JE; Otzen, DE | 1 |
| Cheng, H; Dolgikh, DA; Maki, K; Roder, H; Shastry, MC | 1 |
| Borén, K; Carlsson, U; Grankvist, H; Hammarström, P | 1 |
| Khurana, S; Kumar, N; Parrill, AL; Tomar, A | 1 |
| Brown, RE; Chung, T; Li, XM; Lin, X; Malakhova, ML; Molotkovsky, JG; Pike, HM | 1 |
| Ahn, WS; Kim, SJ; Lee, C; Lee, SC; Lee, SM; Mukhopadhyay, P; Ryu, SE; Storz, G; Yu, MH | 1 |
| Akerfeldt, KS; Berggård, T; Borek, V; Cedervall, T; Linse, S; Thulin, E | 1 |
| Dempsey, CE; Mason, PE; Piggot, TJ | 1 |
| Chant, A; Kneale, GG; Kraemer-Pecore, CM; Watkin, R | 1 |
| Banci, L; Barker, PD; Bruix, M; Ciofi-Baffoni, S; de Lumley Woodyear, T; Fersht, AR; Garcia, P; Johnson, CM; Ramachandra Shastry, MC; Rico, M; Roder, H | 1 |
| Bhattacharyya, M; Chakrabarti, A; Ray, S | 1 |
| Barbiroli, A; Bonomi, F; Caldinelli, L; Fessas, D; Iametti, S; Molla, G; Pilone, MS; Pollegioni, L | 1 |
| Calosci, N; Eklund, L; Engström, A; Gianni, S; Jemth, P; Larsson, M; Malatesta, F; Ngang, CC; Travaglini-Allocatelli, C | 1 |
| Dalessio, PM; Fromholt, SE; Ropson, IJ | 1 |
| Collins, ES; Dobson, CM; Hirai, K; Schwalbe, H; Segawa, S; Tachibana, H; Wirmer, J | 1 |
| de Jongh, HH; Meinders, MB; van Teeffelen, AM | 1 |
| Caldinelli, L; Molla, G; Pilone, MS; Pollegioni, L | 1 |
| Chattopadhyay, K; Frieden, C | 1 |
| Asgeirsson, B; Guojónsdóttir, K | 1 |
| Boyer, JA; Dalessio, PM; Ropson, IJ | 1 |
| Du, D; Gai, F; Tucker, MJ | 1 |
| Anderson, LM; Marqusee, S; Sabogal, A; Wildes, D | 1 |
| Budisa, N; Golbik, R; Lepthien, S; Rubini, M | 1 |
| Ahmad, B; Gull, N; Khan, RH; Kumar, S | 1 |
| Arjara, G; Gray, HB; Kim, JE; Richards, JH; Winkler, JR | 1 |
| Dev, S; K, ND; Sinha, S; Surolia, A | 1 |
| Brunori, M; Gianni, S; Ivarsson, Y; Jemth, P; Malatesta, F; Travaglini-Allocatelli, C | 1 |
| Ahmad, F; Anjum, F; Dar, TA; Islam, A; Moosavi-Movahedi, AA; Singh, LR | 1 |
| Bell, E; Brancaleon, L; Harvey, BJ | 1 |
| Frieden, C; Li, H | 1 |
| Bhattacharya, J; Dasgupta, AK; GhoshMoulick, R; Jasrapuria, S; Sarkar, T | 1 |
| Berne, BJ; Eleftheriou, M; Royyuru, AK; Zhou, R | 1 |
| Bondarenko, PV; Brems, DN; Fachini, RA; Harvey, TS; Ketchem, RR; Kohno, T; Latypov, RF; Liu, D; Raibekas, AA; Rosenfeld, RD | 1 |
| Bandyopadhyay, AK; Krishnamoorthy, G; Padhy, LC; Sonawat, HM | 1 |
| Das, KP; Saha, S | 1 |
| Chen, Y; Kopka, M; Lapidus, LJ; Singh, VR; Wedemeyer, WJ | 1 |
| Alston, RW; Grimsley, GR; Lasagna, M; Pace, CN; Reinhart, GD; Scholtz, JM | 2 |
| Aparicio, R; Bispo, JA; Bonafe, CF; Joekes, I; Santos, JL; Silva, JL | 1 |
| Anderson, E; Cole, JL | 1 |
| Ballard, L; Foguel, D; Rocha, CB; Silva, JL; Sorenson, MM; Suarez, MC; Yu, A | 1 |
| Akanuma, S; Yamagishi, A | 1 |
| Barrick, D; Kloss, E | 1 |
| Gable, JE; Kim, JE; Sanchez, KM; Schlamadinger, DE | 1 |
| Boyer, JA; Dalessio, PM; Ropson, IJ; Schaeffer, BA | 1 |
| Bose, HS; Bose, M; Debnath, D; Whittal, RM | 1 |
| Bellini, T; Buscaglia, M; Longhi, R; Soranno, A | 1 |
| Dwivedi, UN; Goyal, N; Rai, S | 1 |
| Buczko, W; Dadan, J; Myśliwiec, H; Myśliwiec, P; Pawlak, D; Pawlak, K | 1 |
| Cho, AS; Grothe, HL; Huang, AJ; Little, MR; Yuan, C | 1 |
| Carpenter, JF; Randolph, TW; Salinas, BA; Sathish, HA; Shah, AU | 1 |
| Raman Suri, C; Rawat, S; Sahoo, DK | 1 |
| Makhatadze, GI; Streicher, WW; Wafer, LN | 1 |
| Sharp, KA; Vanderkooi, JM; Zelent, B | 1 |
| Hiller, S; Hünenberger, PH; Kräutler, V | 1 |
| Baron, B; Chenal, A; England, P; Karst, JC; Ladant, D; Sotomayor Pérez, AC; Wozniak, AK | 1 |
| Bednárova, L; Heyda, J; Jungwirth, P; Konvalinka, J; Kožíšek, M; Thompson, G; Vondrášek, J | 1 |
| Chen, HL; Chen, JC; Lin, LL; Lo, HF; Su, JY | 1 |
| Chen, KC; Roder, H; Wedemeyer, WJ; Xu, M | 1 |
| Deutz, NE; Engelen, MP; Soeters, PB; Ten Have, GA | 1 |
| Cheung, MS; Samiotakis, A | 1 |
| Adewusi, SR; Falade, OS; Harwood, CE | 1 |
| Bonomi, F; Corredig, M; Iametti, S; Keerati-u-rai, M; Miriani, M | 1 |
| Anand, RP; B Ningappa, M; Balaram, H; Mehrotra, S; Raman, J | 1 |
| Barakat, C; Patra, D; Tafech, RM | 1 |
| Basu, A; Datta, S; Dey, S | 1 |
| Ghosh, G; Mandal, DK | 1 |
| Cernocká, H; Kurzątkowska, K; Ostatná, V; Paleček, E | 1 |
| Kuo, HH; Mauk, AG | 1 |
| Abbas, SA; Gaspar, G; Kalonia, DS; Patapoff, TW; Sharma, VK | 1 |
| Clarke, J; Hill, SA; Rogers, JM; Shammas, SL | 1 |
| Ahmad, E; Ashraf, R; Bhushan, B; Chaturvedi, SK; Khan, JM; Khan, RH; Qadeer, A; Rabbani, G | 1 |
| Cheng, H; Maki, K; Mizukami, T; Roder, H; Xu, M | 1 |
| Podile, AR; Swamy, MJ; Tarafdar, PK; Vedantam, LV | 1 |
| Lu, W; Wang, Z; Wei, Y; Ye, C | 1 |
| Bakkes, PJ; Schmitt, L; Smits, SH; Thomas, S | 1 |
| Kaur, PK; Rashmi, S; Singh, S; Supin, JS | 1 |
| Capozzalo, MM; de Lange, CF; Hampson, DJ; Hansen, CF; Htoo, JK; Kim, JC; Mullan, BP; Pluske, JR; Resink, JW; Stumbles, PA | 1 |
| Chakrabarti, A; Chattopadhyay, A; Chaudhuri, A; Mitra, M; Mukhopadhyay, C; Patra, M | 1 |
| Doepel, L; Hewage, II; Lapierre, H | 1 |
| Bottrel, N; Corum, DG; Drevets, WC; Fiehn, O; Kaddurah-Daouk, R; Motsinger-Reif, A; Rotroff, DM; Salvadore, G; Singh, J | 1 |
| Barioni, MB; Barros, AE; Carvalho, FA; Ito, AS; Tabak, M | 1 |
| Bose, D; Chakrabarti, A; Patra, M | 1 |
| Datta, D; J Swamy, M | 1 |
| Ásgeirsson, B; Hjörleifsson, JG | 1 |
| Dyson, HJ; Sun, X; Wright, PE | 1 |
| Doran, ML; Knee, JM; Li, L; Merritt, TJS; Parkes, TL; Rzezniczak, TZ; Wang, N | 1 |
| An, R; Chen, C; Chen, J; Huang, F; Jiang, Q; Li, M; Li, T; Li, Y; Sun, J; Sun, Z; Tang, Z; Wu, L; Wu, Z; Xu, Q; Yang, J; Yin, Y; Zhang, X; Zhao, S; Zhen, J; Zhu, W | 1 |
| Gadhave, K; Giri, R; Kalita, P; Shukla, H; Tripathi, T | 1 |
| Auriola, S; Hanhineva, K; Heinonen, S; Jääskeläinen, T; Jokkala, J; Kärkkäinen, O; Laivuori, H; Lehtonen, M; Litonius, K | 1 |
| Chen, C; Chen, J; Guo, C; Luo, S; Tong, L; Tong, Y; Xiang, S; Xu, K | 1 |
| Gerasimova, MA; Melnik, BS; Melnik, TN; Nemtseva, EV | 1 |
| Channe Gowda, D; Kumara, HK; Shobha, M; Suhas, R; Suyoga Vardhan, DM | 1 |
| Mahapatra, K; Roy, S | 1 |
| Marsee, JD; Miller, JM; Moore, S; Pickens, A; Rodriguez, JL | 1 |
| Baharifar, H; Faridbod, F; Faridi Majidi, R; Honarvarfard, E; Khorramizadeh, MR; Khoshnevisan, K; Larijani, B; Mahmoudi-Kohan, S; Maleki, H; Mohammadi, H; Saadat, F; Sadeghi Afjeh, M; Sajjadi-Jazi, SM; Torabi, F | 1 |
| Li, F; Li, M; Li, N; Li, X; Liu, L; Qiu, W; Wang, Q; Wang, X; Wu, D; Yu, J | 1 |
| Filimonov, V; Kashparov, I; Lekontseva, N; Marchenko, N; Marchenkov, V; Murina, V; Nikulin, A; Semisotnov, G | 1 |
| Alves da Cunha Valini, G; Alves Marçal, D; Brandão Melo, AD; França, I; Gastmann Brand, H; Hauschild, L; Karpeggiane de Oliveira, MJ; Khun Htoo, J; Reis Furtado Campos, PH; Righetti Arnaut, P; Trevisan Ortiz, M | 1 |
4 review(s) available for urea and tryptophan
| Article | Year |
|---|---|
[Pharmacology of hypnotics].
Topics: Barbiturates; Benzodiazepines; Bromisovalum; Chloral Hydrate; Half-Life; Humans; Hypnotics and Sedatives; Methaqualone; Piperidones; Sleep; Substance-Related Disorders; Tryptophan; Urea | 1976 |
The aqueous pore in the red cell membrane: band 3 as a channel for anions, cations, nonelectrolytes, and water.
Topics: 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; 4-Chloromercuribenzenesulfonate; 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; Animals; Anion Exchange Protein 1, Erythrocyte; Anions; Biological Transport; Cations; Cell Membrane Permeability; Electrolytes; Erythrocyte Membrane; Humans; Hydrogen Bonding; Ion Channels; Membrane Lipids; Models, Molecular; Protein Conformation; Spectrometry, Fluorescence; Sulfhydryl Compounds; Sulfhydryl Reagents; Thermodynamics; Tryptophan; Urea; Water | 1983 |
Amino acid metabolism in liver disease.
Topics: Amino Acids; Amino Acids, Branched-Chain; Ammonia; Animals; Humans; Liver Diseases; Methionine; Nerve Growth Factors; Tryptophan; Urea | 1999 |
AMINO ACID METABOLISM.
Topics: Amino Acid Oxidoreductases; Amino Acids; Carboxy-Lyases; Glutamates; Glycine; Hydroxyproline; Lysine; Peroxidases; Phenylalanine; Proline; Proteins; Pyridoxal Phosphate; Research; Serine; Threonine; Transaminases; Tryptophan; Tyrosine; Urea | 1963 |
6 trial(s) available for urea and tryptophan
| Article | Year |
|---|---|
Intravenous nutrition with amino acid solutions in patients with chronic uraemia.
Topics: Adult; Aged; Amino Acids; Arginine; Diet Therapy; Female; Histidine; Humans; Isoleucine; Leucine; Lysine; Male; Methionine; Middle Aged; Nitrogen; Parenteral Nutrition; Phenylalanine; Threonine; Tryptophan; Urea; Uremia; Valine | 1972 |
Whole-body protein turnover in obese subjects during two very low energy diets of differing amino acid composition.
Topics: Adult; Amino Acids; Analysis of Variance; Body Mass Index; Caseins; Collagen; Diet, Protein-Restricted; Energy Metabolism; Female; Humans; Male; Methionine; Middle Aged; Nitrogen; Nitrogen Isotopes; Obesity; Plant Proteins, Dietary; Proteins; Soybean Proteins; Tryptophan; Urea | 1995 |
Plasma urea appearance rate is lower when children with kwashiorkor and infection are fed egg white-tryptophan rather than milk protein.
Topics: Acute-Phase Proteins; Animals; Child; Child, Preschool; Dietary Proteins; Egg White; Female; Humans; Infant; Infections; Kwashiorkor; Malawi; Male; Milk; Tryptophan; Urea | 2000 |
A psychophysical investigation of binary bitter-compound interactions.
Topics: Adult; Cluster Analysis; Dose-Response Relationship, Drug; Drug Combinations; Drug Synergism; Female; Humans; Individuality; Male; Middle Aged; Phenylalanine; Psychophysics; Quaternary Ammonium Compounds; Ranitidine; Stimulation, Chemical; Taste Buds; Taste Threshold; Tryptophan; Urea | 2003 |
Effect of increasing the dietary tryptophan to lysine ratio on plasma levels of tryptophan, kynurenine and urea and on production traits in weaner pigs experimentally infected with an enterotoxigenic strain of Escherichia coli.
Topics: Animal Feed; Animal Nutritional Physiological Phenomena; Animals; Bacterial Shedding; Body Weight; Diarrhea; Diet; Dose-Response Relationship, Drug; Enterotoxigenic Escherichia coli; Escherichia coli Infections; Feces; Kynurenine; Lysine; Swine; Swine Diseases; Tryptophan; Urea | 2015 |
Increased dietary Trp, Thr, and Met supplementation improves growth performance and protein deposition of salmonella-challenged growing pigs under poor housing conditions.
Topics: Amino Acids; Animal Feed; Animals; Diet; Dietary Supplements; Housing Quality; Methionine; Salmonella typhimurium; Swine; Threonine; Tryptophan; Urea | 2023 |
452 other study(ies) available for urea and tryptophan
| Article | Year |
|---|---|
Cheminformatics analysis of assertions mined from literature that describe drug-induced liver injury in different species.
Topics: Animals; Chemical and Drug Induced Liver Injury; Cluster Analysis; Databases, Factual; Humans; MEDLINE; Mice; Models, Chemical; Molecular Conformation; Quantitative Structure-Activity Relationship | 2010 |
Binding of phosphorylcholine to an IgM Waldenström as studied by fluorescence spectroscopy and circular dichroism.
Topics: Animals; Choline; Circular Dichroism; Guanidines; Humans; Hydrogen-Ion Concentration; Immunoglobulin M; Mice; Myeloma Proteins; Phosphorylcholine; Protein Binding; Protein Denaturation; Spectrometry, Fluorescence; Tryptophan; Urea; Waldenstrom Macroglobulinemia | 1976 |
[Spectroscopic study of the structural changes of scorpion hemocyanin, induced by pH variations and addition of various salts].
Topics: Animals; Anions; Circular Dichroism; Hemocyanins; Hydrogen-Ion Concentration; Macromolecular Substances; Oxygen; Protein Conformation; Scorpions; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Tryptophan; Tyrosine; Urea | 1976 |
Ferricytochrome c chain folding measured by the energy transfer of tryptophan 59 to the heme group.
Topics: Cytochrome c Group; Energy Transfer; Fluorescence; Guanidines; Heme; Hydrogen-Ion Concentration; Kinetics; Protein Conformation; Temperature; Tryptophan; Tyrosine; Urea | 1976 |
Reaction of bovine and equine growth hormones with tetranitromethane.
Topics: Amino Acids; Angiotensin II; Animals; Cattle; Chromatography, Gel; Growth Hormone; Horses; Hydrogen-Ion Concentration; Methane; Molecular Weight; Species Specificity; Tetranitromethane; Time Factors; Tryptophan; Tyrosine; Urea | 1977 |
Circular dichroism studies on the N-bromosuccinimide oxidation of ribonuclease from Aspergillus saitoi.
Topics: Adenosine Monophosphate; Aspergillus; Bromosuccinimide; Circular Dichroism; Endonucleases; Hydrogen-Ion Concentration; Oxidation-Reduction; Protein Binding; Ribonucleases; Succinimides; Tryptophan; Tyrosine; Urea | 1977 |
Proceedings: Comparative effects of N-bromosuccinimide on lysozymes of hen egg-white and of human origin.
Topics: 1-Propanol; Acetylglucosamine; Amino Acids; Animals; Bromosuccinimide; Chickens; Dose-Response Relationship, Drug; Egg White; Electrophoresis, Polyacrylamide Gel; Humans; Hydrogen-Ion Concentration; Micrococcus; Muramidase; Succinimides; Tryptophan; Urea | 1975 |
Use of N-chlorosuccinimide/urea for the selective cleavage of tryptophanyl peptide bonds in proteins. Cytochrome c.
Topics: Amino Acids; Chromatography, Ion Exchange; Cytochrome c Group; Indicators and Reagents; Methods; Succinimides; Tryptophan; Urea | 1977 |
The luminescence properties of concanavalin A.
Topics: Apoproteins; Binding Sites; Calcium; Concanavalin A; Fluorescence; Guanidines; Hydrogen-Ion Concentration; Luminescent Measurements; Magnesium; Methylglucosides; Spectrum Analysis; Temperature; Time Factors; Tryptophan; Tyrosine; Urea | 1975 |
Growth hormone and prolactin responses to thyrotropin-releasing hormone in patients with severe liver disease.
Topics: Adult; Alkaline Phosphatase; Aspartate Aminotransferases; Bilirubin; Blood Glucose; Cholinesterases; Female; Growth Hormone; Hepatitis; Humans; Kinetics; Liver Cirrhosis; Male; Middle Aged; Prolactin; Serum Albumin; Thyrotropin-Releasing Hormone; Tryptophan; Urea | 1977 |
A new aspect of the urinary excretion of tryptophan metabolites in patients with cancer of the bladder.
Topics: 3-Hydroxyanthranilic Acid; Adult; Aged; Aspartate Aminotransferases; Creatinine; Female; Humans; Kidney Neoplasms; Kynurenic Acid; Kynurenine; Male; Middle Aged; ortho-Aminobenzoates; Sodium; Tryptophan; Urea; Ureteral Obstruction; Urinary Bladder Neoplasms; Xanthurenates | 1978 |
Amino acid requirements of the breeding sow. 2. The dietary lysine requirement of the lactating sow.
Topics: Amino Acids; Animals; Diet; Female; Lactation; Lysine; Nutritional Physiological Phenomena; Nutritional Requirements; Pregnancy; Swine; Threonine; Tryptophan; Urea | 1978 |
Renaturation of yeast inorganic pyrophosphatase denatured in urea and guanidine hydrochloride.
Topics: Bromosuccinimide; Dithiothreitol; Glutathione; Guanidines; Macromolecular Substances; Mercaptoethanol; Molecular Weight; Osmolar Concentration; Protein Denaturation; Pyrophosphatases; Saccharomyces cerevisiae; Sulfhydryl Compounds; Tryptophan; Urea | 1975 |
Conformational changes of 30S ribosomes measured by intrinsic and extrinsic fluorescence.
Topics: Circular Dichroism; Escherichia coli; Fluorescence; Magnesium; Maleimides; Protein Conformation; Pyrenes; Ribosomal Proteins; Ribosomes; Tryptophan; Urea | 1976 |
Modification of bovine alpha-lactalbumin with N-bromosuccinimide and 2-hydroxy-5-nitrobenzylbromide.
Topics: 2-Hydroxy-5-nitrobenzyl Bromide; Amino Acids; Animals; Binding Sites; Bromosuccinimide; Cattle; Female; Kinetics; Lactalbumin; Lactose Synthase; Milk; Peptide Fragments; Protein Binding; Protein Conformation; Spectrometry, Fluorescence; Succinimides; Tryptophan; Urea | 1975 |
Fluorescence characteristics of native and denatured monellin.
Topics: Guanidines; Plant Proteins; Protein Binding; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Tryptophan; Tyrosine; Urea | 1977 |
The influence of temperature and urea on intrinsic fluorescence of Streptomyces subtilisin inhibitor. A study by fluorescence polarization and quenching.
Topics: Bacterial Proteins; Binding Sites; Kinetics; Mathematics; Protein Binding; Protein Conformation; Spectrometry, Fluorescence; Streptomyces; Subtilisins; Temperature; Thermodynamics; Tryptophan; Tyrosine; Urea | 1977 |
Determination of the optimum dietary proportions of lysine and tryptophan for growing rats based on growth, food intake and plasma metabolites.
Topics: Age Factors; Animals; Diet; Female; Lysine; Male; Nutritional Requirements; Rats; Species Specificity; Tryptophan; Urea | 1977 |
Determination of the optimum dietary proportions of lysine and tryptophan for growing pigs based on growth, food intake and plasma metabolites.
Topics: Age Factors; Animals; Diet; Female; Lysine; Male; Nutritional Requirements; Species Specificity; Swine; Tryptophan; Urea | 1977 |
Protein synthesis in the hepatocyte.
Topics: Albumins; Animals; Arginine; Carbon Radioisotopes; Cell Aggregation; DNA; Ethanol; Fasting; Liver; Lysine; Ornithine; Perfusion; Phenylalanine; Polyribosomes; Rabbits; RNA, Ribosomal; Serum Albumin; Tritium; Tryptophan; Urea | 1975 |
Structural alterations in the 30 S ribosomal subunit of Escherichia coli observed with the fluorescent probe N-(3-pyrene) maleimide.
Topics: Escherichia coli; Fluorescent Dyes; Magnesium; Maleimides; Pyrenes; Ribosomes; Tryptophan; Urea | 1975 |
Comparison between the metabolic effects of tryptophan and histidine deficiencies in the rat.
Topics: Animal Nutritional Physiological Phenomena; Animals; Body Water; Body Weight; Deficiency Diseases; Histidine; Kidney; Liver; Male; Muscles; Nitrogen; Organ Size; Phenylalanine; Proteins; Rats; Tryptophan; Urea | 1975 |
Isolation and characterization of a variant of ovomucoid.
Topics: Animals; Chemical Precipitation; Chickens; Chromatography, Gel; Egg Proteins; Electrophoresis, Polyacrylamide Gel; Molecular Weight; Ovalbumin; Ovomucin; Protein Conformation; Rheology; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Tryptophan; Urea; Viscosity | 1975 |
Responses of growing pigs to combinations of essential amino acids.
Topics: Amino Acids, Essential; Animals; Female; Hordeum; Isoleucine; Lysine; Methionine; Swine; Threonine; Tryptophan; Urea | 1975 |
Characteristics of murine protoporphyrinogen oxidase.
Topics: Animals; Circular Dichroism; Flavin Mononucleotide; Flavoproteins; Kinetics; Mice; Mitochondria, Liver; Mitochondrial Proteins; Oxidoreductases; Oxidoreductases Acting on CH-CH Group Donors; Protein Conformation; Protein Denaturation; Protoporphyrinogen Oxidase; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Tryptophan; Urea | 1992 |
Conformational stability of bovine alpha-crystallin. Evidence for a destabilizing effect of ascorbate.
Topics: Acrylamide; Acrylamides; Animals; Ascorbic Acid; Cattle; Crystallins; Guanidine; Guanidines; Hot Temperature; Hydrogen-Ion Concentration; In Vitro Techniques; Iodides; Light; Nephelometry and Turbidimetry; Protein Conformation; Protein Denaturation; Scattering, Radiation; Spectrometry, Fluorescence; Tryptophan; Urea | 1992 |
The uncoupling protein from brown adipose tissue mitochondria. The environment of the tryptophan residues as revealed by quenching of the intrinsic fluorescence.
Topics: Adipose Tissue, Brown; Animals; Carrier Proteins; Cricetinae; Guanidine; Guanidines; Hydrochloric Acid; Ion Channels; Mathematics; Membrane Proteins; Mitochondria; Mitochondrial Proteins; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Tryptophan; Uncoupling Protein 1; Urea | 1992 |
Urea denaturation of barnase: pH dependence and characterization of the unfolded state.
Topics: Bacterial Proteins; Disulfides; Hydrogen-Ion Concentration; Protein Conformation; Protein Denaturation; Ribonuclease T1; Ribonucleases; Tryptophan; Tyrosine; Urea | 1992 |
Multiphasic denaturation of the lambda repressor by urea and its implications for the repressor structure.
Topics: DNA-Binding Proteins; Escherichia coli; Kinetics; Peptide Fragments; Protein Conformation; Protein Denaturation; Repressor Proteins; Spectrometry, Fluorescence; Sulfhydryl Compounds; Transcription Factors; Tryptophan; Urea; Viral Proteins; Viral Regulatory and Accessory Proteins | 1992 |
Fluorescence studies on the interactions of myelin basic protein in electrolyte solutions.
Topics: Acrylamide; Acrylamides; Animals; Brain Chemistry; Cattle; Diffusion; Electrolytes; Fluorescence; Macromolecular Substances; Myelin Basic Protein; Protein Conformation; Spectrum Analysis; Tryptophan; Urea | 1991 |
Unfolding of the regulatory subunit of cAMP-dependent protein kinase I.
Topics: Animals; Cattle; Cyclic AMP; Iodides; Protein Conformation; Protein Denaturation; Protein Kinases; Spectrometry, Fluorescence; Tryptophan; Urea | 1991 |
Monomer-tetramer equilibrium of the Escherichia coli ssb-1 mutant single strand binding protein.
Topics: DNA-Binding Proteins; Escherichia coli; Fluorescence Polarization; Guanidine; Guanidines; Kinetics; Macromolecular Substances; Magnesium Chloride; Molecular Structure; Mutation; Osmolar Concentration; Sodium Chloride; Temperature; Thermodynamics; Tryptophan; Urea | 1991 |
Tryptophan fluorescence in electron-transfer flavoprotein:ubiquinone oxidoreductase: fluorescence quenching by a brominated pseudosubstrate.
Topics: Acrylamide; Acrylamides; Animals; Cesium; Chlorides; Electron-Transferring Flavoproteins; Fatty Acid Desaturases; Flavoproteins; Hydrogen-Ion Concentration; In Vitro Techniques; Iron-Sulfur Proteins; Kinetics; Multienzyme Complexes; Oxidation-Reduction; Oxidoreductases Acting on CH-NH Group Donors; Sodium Iodide; Spectrometry, Fluorescence; Structure-Activity Relationship; Submitochondrial Particles; Swine; Tryptophan; Ubiquinone; Urea | 1991 |
Intrinsic fluorescence of a truncated Bordetella pertussis adenylate cyclase expressed in Escherichia coli.
Topics: Adenylyl Cyclases; Amino Acid Sequence; Bacterial Proteins; Bordetella pertussis; Calmodulin; Escherichia coli; Fluorescence; Molecular Sequence Data; Recombinant Fusion Proteins; Tryptophan; Urea | 1990 |
Effects of a tryptophan supplemented diet and U.V. radiation on the rat lens.
Topics: Animals; Ascorbic Acid; Crystallins; Diet; Glucosephosphate Dehydrogenase; Glutathione; Glutathione Peroxidase; Glutathione Reductase; Lens, Crystalline; Malondialdehyde; Phosphogluconate Dehydrogenase; Rats; Rats, Inbred Strains; Tryptophan; Ultraviolet Rays; Urea | 1990 |
Stable intermediates can be trapped during the reversible refolding of urea-denatured rhodanese.
Topics: Dithionitrobenzoic Acid; Enzyme Stability; Kinetics; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Sulfurtransferases; Thiosulfate Sulfurtransferase; Time Factors; Tryptophan; Urea | 1990 |
Effects of an atypical barbiturate (valofan) on spontaneous and stimulated locomotor activity and on brain serotonin metabolism in mice.
Topics: Animals; Brain; Dextroamphetamine; Hydroxyindoleacetic Acid; Male; Methoxydimethyltryptamines; Mice; Motor Activity; Serotonin; Tranylcypromine; Tryptophan; Urea | 1987 |
Urea-induced inactivation, dissociation, and unfolding of the allosteric phosphofructokinase from Escherichia coli.
Topics: Allosteric Regulation; Chromatography, High Pressure Liquid; Escherichia coli; Guanidine; Guanidines; Kinetics; Phosphofructokinase-1; Protein Conformation; Protein Denaturation; Thiocyanates; Tryptophan; Urea | 1989 |
UV spectroscopic studies of human erythrocyte superoxide dismutase.
Topics: Apoenzymes; Azides; Copper; Cyanides; Erythrocytes; Ethylene Glycol; Ethylene Glycols; Humans; Isoenzymes; Phenylalanine; Potassium Chloride; Spectrophotometry, Ultraviolet; Superoxide Dismutase; Tryptophan; Urea; Zinc | 1989 |
Tryptic digestion of bovine secretory IgA at elevated temperature and in urea. Isolation of SC domain 1 which is covalently bound to IgA dimer and binds non-covalently to IgM.
Topics: Alkylation; Animals; Cattle; Chromatography, Gel; Chromatography, High Pressure Liquid; Electrophoresis, Polyacrylamide Gel; Hot Temperature; Immunoglobulin A; Immunoglobulin A, Secretory; Immunoglobulin Fragments; Immunoglobulin M; Iodobenzoates; Oxidation-Reduction; Secretory Component; Trypsin; Tryptophan; Urea | 1989 |
Characterization of lens alpha-crystallin tryptophan microenvironments by room temperature phosphorescence spectroscopy.
Topics: Acrylamide; Acrylamides; Crystallins; Guanidine; Guanidines; Iodides; Luminescent Measurements; Nitrites; Protein Conformation; Protein Denaturation; Spectrum Analysis; Temperature; Thermodynamics; Tryptophan; Urea | 1989 |
Interaction of Cibacron Blue F3GA with glutamine synthetase: use of the dye as a conformational probe. 1. Studies using unfractionated dye samples.
Topics: Adenine Nucleotides; Coloring Agents; Escherichia coli; Glutamate-Ammonia Ligase; Kinetics; Osmolar Concentration; Oxidation-Reduction; Protein Binding; Protein Conformation; Salts; Spectrophotometry; Triazines; Tryptophan; Urea | 1985 |
Folding-unfolding and aggregation-dissociation of bovine alpha-crystallin subunits; evidence for unfolding intermediates of the alpha A subunits.
Topics: Animals; Cattle; Crystallins; In Vitro Techniques; Isoelectric Focusing; Protein Binding; Protein Conformation; Spectrometry, Fluorescence; Tryptophan; Urea | 1989 |
Effects of urea and guanidine hydrochloride on the activity and dynamical structure of equine liver alcohol dehydrogenase.
Topics: Alcohol Dehydrogenase; Alcohol Oxidoreductases; Animals; Circular Dichroism; Guanidine; Guanidines; Horses; Kinetics; Liver; Luminescent Measurements; Oxidation-Reduction; Protein Conformation; Spectrometry, Fluorescence; Tryptophan; Urea; Viscosity | 1986 |
The protein core of the largest proteoglycan monomer of articular cartilage. Gel electrophoretic pattern and tryptophanyl peptide bond cleavage.
Topics: Animals; beta-Galactosidase; Carbohydrate Conformation; Cartilage, Articular; Chondroitin Lyases; Electrophoresis, Polyacrylamide Gel; Glycoside Hydrolases; Hyaluronic Acid; Molecular Weight; Papio; Peptides; Proteoglycans; Succinimides; Tryptophan; Urea | 1987 |
Are the classical markers sufficient to describe uremic solute accumulation in dialyzed patients? Hippurates reconsidered.
Topics: Chromatography, High Pressure Liquid; Creatinine; Hippurates; Humans; Hypoxanthine; Hypoxanthines; Indoleacetic Acids; Metabolic Clearance Rate; Phosphates; Renal Dialysis; Tryptophan; Urea; Uremia; Uric Acid | 1988 |
Unfolding of the trp repressor from Escherichia coli monitored by fluorescence, circular dichroism and nuclear magnetic resonance.
Topics: Bacterial Proteins; Circular Dichroism; Escherichia coli; Magnetic Resonance Spectroscopy; Protein Conformation; Protein Denaturation; Repressor Proteins; Spectrometry, Fluorescence; Thermodynamics; Transcription Factors; Tryptophan; Urea | 1987 |
Quenching of red cell tryptophan fluorescence by mercurial compounds.
Topics: Anion Exchange Protein 1, Erythrocyte; Biological Transport; Body Water; Chloromercuribenzoates; Depression, Chemical; Dose-Response Relationship, Drug; Erythrocyte Membrane; Ethylmaleimide; Fluorescence; Humans; Kinetics; p-Chloromercuribenzoic Acid; Phenylmercuric Acetate; Protein Binding; Tryptophan; Urea | 1986 |
Tryptophan fluorescence studies of plasma fibronectin: effects of environmental factors.
Topics: Fibronectins; Humans; Hydrogen-Ion Concentration; Protein Conformation; Spectrometry, Fluorescence; Tryptophan; Urea | 1987 |
Urea-induced structural transformations in bovine serum albumin.
Topics: Kinetics; Protein Conformation; Serum Albumin, Bovine; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Tryptophan; Urea | 1987 |
Automated procedure for simultaneously measuring total globulin and total protein in serum.
Topics: Animals; Autoanalysis; Blood Protein Electrophoresis; Blood Proteins; Cattle; Chemical Phenomena; Chemistry; Coloring Agents; Densitometry; gamma-Globulins; Glyoxylates; Humans; Indicators and Reagents; Methods; Protein Binding; Serum Albumin; Serum Albumin, Bovine; Serum Globulins; Spectrophotometry; Tryptophan; Urea | 1971 |
Effects on net plasma protein synthesis of removal of L-tryptophan or L-threonine from a complete amino acid mixture: studies in the isolated perfused rat liver.
Topics: Alpha-Globulins; Amino Acids; Animals; Bile; Blood Proteins; Carbon Isotopes; Diet; Fasting; Fibrinogen; Glucose; Glycoproteins; Haptoglobins; Liver; Lysine; Nitrogen; Perfusion; Rats; Serum Albumin; Threonine; Tryptophan; Urea | 1971 |
Isolation of large peptides by flat-bed polyacrylamide gel electrophoresis.
Topics: Acrylamides; Alkylation; Animals; Cattle; Cyanogen Bromide; Dithiothreitol; Egg White; Electrophoresis; Escherichia coli; Gels; Hydro-Lyases; Iodoacetates; Methods; Muramidase; Peptides; Serum Albumin, Bovine; Staining and Labeling; Tryptophan; Urea | 1972 |
Experimental allergic encephalomyelitis (EAE).
Topics: Amino Acids; Animals; Antigens; Encephalomyelitis, Autoimmune, Experimental; Guinea Pigs; Mice; Myelin Basic Protein; Pepsin A; Rats; Skin Tests; Trypsin; Tryptophan; Urea | 1971 |
Implication of a tryptophyl residue in the active site of dihydrofolate reductase.
Topics: Amino Acids; Binding Sites; Chromatography, Gel; Chromatography, Ion Exchange; Dialysis; Drug Resistance, Microbial; Electrophoresis, Polyacrylamide Gel; Folic Acid Antagonists; Lacticaseibacillus casei; Methotrexate; NADP; Oxidation-Reduction; Protein Denaturation; Spectrophotometry, Ultraviolet; Succinimides; Tetrahydrofolate Dehydrogenase; Tryptophan; Urea | 1974 |
[The biochemical differential diagnosis in the Klebsiella group (author's transl)].
Topics: Bacteriological Techniques; Biochemical Phenomena; Biochemistry; Carbenicillin; Cephalothin; Citrates; Coloring Agents; Gentamicins; Glucose; Klebsiella; Lactose; Lysine; Microbial Sensitivity Tests; Penicillin Resistance; Serotyping; Tetracycline; Tryptophan; Urea | 1974 |
Circular dichroism studies on native fetuin and some of its derivatives.
Topics: Alpha-Globulins; Animals; Cattle; Detergents; Hydrogen-Ion Concentration; Iodoacetates; Neuraminidase; Oxidation-Reduction; Protein Denaturation; Spectrum Analysis; Sulfates; Sulfides; Tryptophan; Tyrosine; Ultraviolet Rays; Urea | 1969 |
Differences in tryptophan exposure between chicken egg-white lysozyme and bovine alpha-lactalbumin.
Topics: Albumins; Alkylation; Animals; Cattle; Chemical Phenomena; Chemistry; Chickens; Muramidase; Niacinamide; Ovalbumin; Oxidation-Reduction; Spectrophotometry; Tryptophan; Urea | 1970 |
Tryptophan as intermediate in dehydrogenase action. 3. Evidence for complete cycle of hydrogen transfer between substrate and tryptophanyl residues in rabbit muscle lactate dehydrogenase.
Topics: Animals; In Vitro Techniques; L-Lactate Dehydrogenase; Lactates; Muscles; NAD; Perchlorates; Rabbits; Tritium; Tryptophan; Urea | 1967 |
The chemistry of flavines and flavorproteins. Photoreduction of flavines by amino acids.
Topics: Aldehydes; Benzene Derivatives; Carbon Dioxide; Dimethyl Sulfoxide; Edetic Acid; Flavin Mononucleotide; Flavin-Adenine Dinucleotide; Flavins; Glycine; Kinetics; Light; NAD; Oxidation-Reduction; Phenylacetates; Potassium Iodide; Riboflavin; Serotonin; Temperature; Tryptophan; Urea | 1968 |
Properties of pure acyl phosphatase from bovine brain.
Topics: Amino Acids; Animals; Brain; Cattle; Chemical Phenomena; Chemistry; Chromatography, Gel; Electrophoresis, Disc; Hydrogen-Ion Concentration; Lysine; Methods; Molecular Weight; Osmolar Concentration; Phosphoric Monoester Hydrolases; Sulfhydryl Compounds; Tryptophan; Tyrosine; Urea | 1969 |
Relation between conformations and activities of lipoamide dehydrogenase. IV. Apoenzyme structure and flavin binding aspects.
Topics: Adenine; Binding Sites; Chemical Phenomena; Chemistry; Chloromercuribenzoates; Cold Temperature; Dihydrolipoamide Dehydrogenase; Diphosphates; Flavin Mononucleotide; Flavin-Adenine Dinucleotide; Flavins; Fluorometry; Halogens; Hot Temperature; Hydrogen-Ion Concentration; Osmolar Concentration; Protein Binding; Protein Denaturation; Time Factors; Tryptophan; Tyrosine; Urea | 1970 |
Conversion of neutral to alkaline liver fructose 1,6-bisphosphatase: changes in molecular properties of the enzyme.
Topics: Adenosine Monophosphate; Allosteric Regulation; Amino Acids; Aminopeptidases; Animals; Catalysis; Fructose-Bisphosphatase; Fructose-Bisphosphate Aldolase; Hexosediphosphates; Hydrogen-Ion Concentration; Liver; Molecular Conformation; Rabbits; Spectrometry, Fluorescence; Structure-Activity Relationship; Subtilisins; Tryptophan; Urea | 1973 |
Effect of alcohol on albumin synthesis by the isolated perfused rat liver.
Topics: Albumins; Amino Acids, Essential; Animal Nutritional Physiological Phenomena; Animals; Carbon Radioisotopes; Carbonates; Ethanol; In Vitro Techniques; Kinetics; Liver; Male; Methylene Blue; NAD; Oxidation-Reduction; Perfusion; Rats; Sodium; Tryptophan; Urea | 1973 |
The Trp-59 fluorescence of ferricytochrome c as a sensitive measure of the over-all protein conformation.
Topics: Amino Acid Sequence; Animals; Chemical Phenomena; Chemistry; Cytochrome c Group; Guanidines; Horses; Hydrogen-Ion Concentration; Iron; Ligands; Methionine; Myocardium; Protein Binding; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Tryptophan; Urea; Viscosity | 1974 |
Cooperative interactions between native and modified subunits of rabbit liver fructose 1,6-bisphosphatase.
Topics: Adenosine Monophosphate; Allosteric Regulation; Animals; Binding Sites; Electrophoresis, Disc; Enzyme Activation; Fructose-Bisphosphatase; Hydrogen-Ion Concentration; Kinetics; Liver; Macromolecular Substances; Mathematics; Protein Binding; Protein Conformation; Rabbits; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Subtilisins; Tryptophan; Urea | 1974 |
Further investigation of a variant of the placental alkaline phosphatase in human hepatic carcinoma.
Topics: Alkaline Phosphatase; Animals; Carcinoma, Hepatocellular; Chromatography, Gel; Cross Reactions; Edetic Acid; Electrophoresis, Polyacrylamide Gel; Hot Temperature; Humans; Hydrogen-Ion Concentration; Immunodiffusion; Isoenzymes; Leucine; Liver Neoplasms; Molecular Weight; Phenylalanine; Placenta; Rabbits; Tryptophan; Urea | 1974 |
Effect in man of the addition of tryptophan oniacin to the diet on the excretion of their metabolites.
Topics: Creatinine; Diet; Female; Humans; Kynurenic Acid; NAD; NADP; Niacinamide; Nicotinic Acids; Nitrogen; Pyridones; Tryptophan; Urea; Xanthurenates | 1969 |
[Studies on the binding of plant growth substances to several components of chromatin in vitro. I].
Topics: Binding Sites; DNA; Gibberellins; Hydrogen-Ion Concentration; Indoleacetic Acids; Plant Growth Regulators; Sodium Chloride; Spectrophotometry; Tryptophan; Urea | 1971 |
A spectroscopic study of bovine lactoferrin.
Topics: Acetates; Animals; Binding Sites; Cats; Circular Dichroism; Colostrum; Dimethyl Sulfoxide; Electrophoresis, Polyacrylamide Gel; Ethylene Glycols; Female; Glycerol; Guanidines; Iron; Lactoferrin; Lactoglobulins; Mammary Glands, Animal; Milk; Pregnancy; Protein Binding; Protein Conformation; Solvents; Spectrophotometry; Spectrophotometry, Ultraviolet; Sucrose; Tryptophan; Tyrosine; Urea | 1974 |
Hepatitis B surface antigen (HBs Ag): tryptophan content and biological activity.
Topics: Bromine; Hepatitis B Antigens; Hydrogen-Ion Concentration; Molecular Weight; Radioimmunoassay; Spectrophotometry; Succinimides; Tryptophan; Tyrosine; Urea; Viral Proteins | 1974 |
[Amino acid levels in the blood serum of patients with chronic colitis].
Topics: Adult; Alanine; Amino Acids; Ammonia; Arginine; Aspartic Acid; Betaine; Chronic Disease; Citrulline; Colitis; Glutamates; Glutamine; Glycine; Histidine; Humans; Middle Aged; Ornithine; Phenylalanine; Proline; Serine; Taurine; Threonine; Tryptophan; Tyrosine; Urea | 1974 |
An improved column chromatographic method for isolation of tryptophan metabolites.
Topics: 5-Hydroxytryptophan; Aldehydes; Amides; Animals; Chromatography, DEAE-Cellulose; Colorimetry; Hydroxyindoleacetic Acid; Indoleacetic Acids; Kynurenic Acid; Kynurenine; Male; Methods; Nitriles; Rats; Serotonin; Spectrometry, Fluorescence; Tryptamines; Tryptophan; Urea; Vitamin B 6 Deficiency; Xanthurenates | 1972 |
Effect of pH and temperature on nitrosamide-induced mutation in Escherichia coli.
Topics: Amides; Cell Survival; Escherichia coli; Genetic Code; Guanidines; Hydrogen-Ion Concentration; Leucine; Methods; Mutagens; Mutation; Nitroso Compounds; Nitrosoguanidines; Nitrosourea Compounds; Temperature; Time Factors; Tryptophan; Urea | 1972 |
Tryptophan fluorescence study of conformational transitions of the oxidized and reduced form of thioredoxin.
Topics: Amino Acid Sequence; Amino Acids; Bacterial Proteins; Dioxins; Disulfides; Dithiothreitol; Escherichia coli; Ethanol; Guanidines; Hydrogen-Ion Concentration; Oxidation-Reduction; Plant Proteins; Protein Conformation; Protein Denaturation; Saccharomyces; Species Specificity; Spectrometry, Fluorescence; Tryptophan; Urea | 1972 |
[Study on the fluorescence of Escherichia coli alkaline phosphatase. I. Properties of the excited state].
Topics: Alkaline Phosphatase; Amides; Amino Acid Sequence; Amino Acids; Chemical Phenomena; Chemistry; Dioxins; Escherichia coli; Fluorescence; Peptides; Potassium Iodide; Protein Denaturation; Spectrometry, Fluorescence; Spectrophotometry; Time Factors; Tryptophan; Tyrosine; Ultraviolet Rays; Urea | 1972 |
Nucleoside triphosphate-nucleoside diphosphate transphosphorylase (nucleoside diphosphokinase). 3. Subunit structure of the crystalline enzyme from brewers' yeast.
Topics: Amino Acids; Chromatography, Gel; Crystallization; Cysteine; Diffusion; Electrophoresis, Polyacrylamide Gel; Hydrazines; Kinetics; Macromolecular Substances; Mathematics; Molecular Weight; Nucleotides; Peptides; Phosphotransferases; Saccharomyces cerevisiae; Sodium Dodecyl Sulfate; Sulfhydryl Compounds; Trypsin; Tryptophan; Ultracentrifugation; Urea | 1973 |
Limited pepsin digestion of bovine plasma albumin.
Topics: Alkylation; Amino Acids; Animals; Binding Sites; Binding, Competitive; Caprylates; Carbon Isotopes; Cattle; Chromatography, DEAE-Cellulose; Chromatography, Gel; Cyanogen Bromide; Dialysis; Electrophoresis, Disc; Isoelectric Focusing; Molecular Weight; Oxidation-Reduction; Pepsin A; Peptides; Protein Binding; Serum Albumin, Bovine; Sodium Dodecyl Sulfate; Stereoisomerism; Tryptophan; Urea | 1973 |
Bibliography of paper and thin-layer chromatography 1966-1969. Amines, amides, and related nitrogen compounds.
Topics: Amides; Amines; Amino Alcohols; Bibliographies as Topic; Catecholamines; Choline; Chromatography; Chromatography, Paper; Chromatography, Thin Layer; Diazonium Compounds; Guanidines; Hydrazines; Methods; Nitriles; Nitro Compounds; Nitrogen; Nitrosamines; ortho-Aminobenzoates; Oximes; Sphingosine; Tryptophan; Urea | 1972 |
Protein metabolism in renal failure.
Topics: Amino Acids, Essential; Ammonia; Arginine; Blood Urea Nitrogen; Diet; Dietary Carbohydrates; Dietary Fats; Dietary Proteins; Eggs; Hemoglobins; Histidine; Humans; Intestinal Absorption; Kidney Failure, Chronic; Kidney Transplantation; Nitrogen Isotopes; Nutritional Requirements; Phenylalanine; Renal Dialysis; Transplantation, Homologous; Tryptophan; Urea; Valine | 1973 |
Quenching of the fluorescence of proteins by silver nitrate.
Topics: Alcohol Oxidoreductases; Binding Sites; Cysteine; Fluorescence; Hydrogen-Ion Concentration; Liver; Mathematics; Osmolar Concentration; Ovalbumin; Protein Binding; Proteins; Saccharomyces cerevisiae; Silver Nitrate; Species Specificity; Spectrometry, Fluorescence; Spectrophotometry; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds; Time Factors; Trypsinogen; Tryptophan; Urea | 1973 |
Complementation in vitro between mutationally altered beta2 subunits of Escherichia coli tryptophan synthetase.
Topics: Cell-Free System; Chromosome Mapping; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Genes; Genetic Complementation Test; Hybridization, Genetic; Hydrogen-Ion Concentration; Methods; Mutation; Recombination, Genetic; Tryptophan; Tryptophan Synthase; Urea | 1974 |
An analysis of the causes of the different light sensitivities of tryptophan residues in some proteins.
Topics: Chymotrypsinogen; Cystine; Dose-Response Relationship, Radiation; Humans; In Vitro Techniques; Muramidase; Pepsin A; Photolysis; Protein Denaturation; Proteins; Radiation Effects; Spectrometry, Fluorescence; Structure-Activity Relationship; Thioglycolates; Trypsin; Tryptophan; Ultraviolet Rays; Urea | 1974 |
In vitro assembly of L-asparaginase subunits.
Topics: Asparaginase; Asparagine; Aspartic Acid; Escherichia coli; Hydrogen-Ion Concentration; Hydrolysis; Macromolecular Substances; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Stereoisomerism; Time Factors; Tryptophan; Tyrosine; Ultracentrifugation; Urea | 1974 |
Intrinsic fluorescence of actin.
Topics: Actins; Adenosine Triphosphate; Animals; Copper; Drug Stability; Edetic Acid; Energy Transfer; Fluorescence; Hot Temperature; Kinetics; Light; Muscle Proteins; Protein Binding; Protein Denaturation; Rabbits; Scattering, Radiation; Spectrometry, Fluorescence; Tryptophan; Tyrosine; Urea | 1972 |
The purification and binding properties of a human thyroxine-binding prealbumin.
Topics: Amino Acids; Buffers; Carbohydrates; Chromatography, DEAE-Cellulose; Chromatography, Gel; Dialysis; Freeze Drying; Glycoproteins; Humans; Hydrogen-Ion Concentration; Hydrolysis; Immunoelectrophoresis; Methods; Pronase; Protein Binding; Serum Albumin; Thyroxine; Thyroxine-Binding Proteins; Triiodothyronine; Tryptophan; Urea | 1972 |
The location of tryptophanyl groups in human and bovine carbonic anhydrases. Ultraviolet difference spectra and chemical modification.
Topics: Animals; Carbonic Anhydrases; Cattle; Circular Dichroism; Crystallization; Deuterium; Dimethyl Sulfoxide; Erythrocytes; Glycerol; Glycols; Guanidines; Humans; Hydrogen-Ion Concentration; Isoenzymes; Models, Structural; Nitrobenzenes; Phenols; Polyethylenes; Protein Denaturation; Species Specificity; Spectrophotometry, Ultraviolet; Sucrose; Tryptophan; Urea | 1973 |
Effects of carbon tetrachloride on albumin synthesis.
Topics: Albumins; Aniline Compounds; Animals; Antioxidants; Carbon Isotopes; Carbon Tetrachloride; Carbonates; Cortisone; Depression, Chemical; DNA; Fasting; Lactates; Liver; Male; Perfusion; Polyribosomes; Pyruvates; Rabbits; RNA; Tryptophan; Urea | 1972 |
The effects of amino acids on albumin synthesis by the isolated perfused rat liver.
Topics: Albumins; Amino Acids; Aminoisobutyric Acids; Animals; Carbon Isotopes; Glucagon; In Vitro Techniques; Liver; Male; Perfusion; Rats; Serum Albumin; Starvation; Tryptophan; Urea | 1972 |
Studies on the low molecular weight protein of myosin.
Topics: Amino Acids; Animals; Chickens; Electrophoresis, Disc; Gels; Molecular Weight; Myosins; Phenylalanine; Sodium Dodecyl Sulfate; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Tryptophan; Tyrosine; Urea | 1972 |
Fluorescence quenching, a tool for probing conformational changes in glycogen phosphorylase.
Topics: Animals; Apoproteins; Fluorescence; Iodides; Kinetics; Methods; Muscles; Myocardium; Phosphorylases; Protein Conformation; Pyridoxal Phosphate; Rabbits; Spectrometry, Fluorescence; Spectrum Analysis; Swine; Tryptophan; Urea | 1973 |
Crayfish ventral nerve cord and hemolymph: content of free amino acids and other metabolites.
Topics: Amino Acids; Aminobutyrates; Ammonia; Animals; Asparagine; Astacoidea; Chromatography, Ion Exchange; Citrulline; Female; Glucose; Glutamates; Glutamine; Hemolymph; In Vitro Techniques; Male; Neurons; Sex Factors; Tryptophan; Urea | 1973 |
Effects of chemical modification on the activity of Crotalus adamanteus Phospholipase A 2 . Evidence for an essential amino group.
Topics: Acylation; Amino Acids; Anhydrides; Animals; Binding Sites; Chromatography, Gel; Ethyl Ethers; Fluorine; Formates; Iodine; Iodoacetates; Lysine; Macromolecular Substances; Nitrobenzenes; Oxidation-Reduction; Phospholipases; Protein Conformation; Snakes; Spectrophotometry, Ultraviolet; Structure-Activity Relationship; Succinimides; Tryptophan; Urea | 1973 |
Conformation measurements on bovine trypsin and alpha-chymotrypsin during photodynamic inactivation.
Topics: Animals; Cattle; Chromatography, Ion Exchange; Chymotrypsin; Coloring Agents; Fluorescence; Hot Temperature; Oxidation-Reduction; Photochemistry; Protein Conformation; Trypsin; Tryptophan; Urea | 1973 |
[Biochemical and immunological properties of human placental lactogen (hPL, human chorionic somatomammotropin: hCS). 2. Biochemical properties of hPL (hCS)].
Topics: Amino Acid Sequence; Amino Acids; Aminopeptidases; Chemical Phenomena; Chemistry; Chromatography, Gel; Cross Reactions; Female; Hot Temperature; Humans; Hydrogen-Ion Concentration; Immune Sera; Molecular Weight; Placental Lactogen; Pregnancy; Spectrum Analysis; Tryptophan; Ultracentrifugation; Urea | 1973 |
Luminescence of the tryptophan and tyrosine residues of trypsin.
Topics: Amino Acid Sequence; Binding Sites; Binding, Competitive; Deuterium; Fluorescence; Iodine Radioisotopes; Luminescent Measurements; Protein Conformation; Protein Denaturation; Sodium Dodecyl Sulfate; Spectrometry, Fluorescence; Structure-Activity Relationship; Temperature; Thermodynamics; Time Factors; Trypsin; Trypsin Inhibitors; Tryptophan; Tyrosine; Urea | 1973 |
Study of albumin synthesis in relation to urea synthesis.
Topics: Albumins; Animals; Arginine; Carbon Radioisotopes; Ethanol; Immunoassay; Liver; Ornithine; Perfusion; Rabbits; Serum Albumin; Tryptophan; Urea | 1973 |
Effect of copper status on the urinary excretion of tryptophan metabolites and amino acids by the rat.
Topics: Amino Acids; Animal Nutritional Physiological Phenomena; Animals; Body Weight; Copper; Deficiency Diseases; Heart; Hemoglobins; Kidney; Kynurenic Acid; Liver; Male; Organ Size; Pyridoxal Phosphate; Rats; Spleen; Taurine; Tryptophan; Urea; Xanthurenates | 1973 |
Flavin-protein interactions in flavoenzymes. Studies of FMN binding in Azotobacter flavodoxin using protein fluorescence lifetime measurements.
Topics: Apoproteins; Azotobacter; Bacterial Proteins; Binding Sites; Bromine; Flavin Mononucleotide; Flavoproteins; Fluorometry; Kinetics; Lasers; Oxidation-Reduction; Protein Binding; Succinimides; Tryptophan; Urea | 1973 |
Urea excretion in the pig: an indicator of protein quality and amino acid requirements.
Topics: Amino Acids; Animal Nutritional Physiological Phenomena; Animals; Blood Urea Nitrogen; Castration; Diet; Dietary Proteins; Evaluation Studies as Topic; Feeding Behavior; Lysine; Male; Methods; Nitrogen; Nutritional Requirements; Plant Proteins; Swine; Time Factors; Tryptophan; Urea; Zea mays | 1974 |
Structure and activity of hepatitis B antigen (HBAg). 2. Amino acid composition of an active subunit of HBAg.
Topics: Amino Acids; Centrifugation, Zonal; Electrophoresis, Paper; Hepatitis B Antigens; Humans; Mercaptoethanol; Molecular Weight; Peptides; Plasma; Radioimmunoassay; Tryptophan; Ultrasonics; Urea | 1974 |
Solvent perturbation difference spectra of a purified sheep antibody.
Topics: Animals; Antibodies; Dimethyl Sulfoxide; Dithiothreitol; Ethylenes; Glycerol; Glycols; Models, Chemical; Polyethylene Glycols; Precipitin Tests; Sheep; Solvents; Spectrophotometry; Tryptophan; Tyrosine; Urea | 1974 |
Studies of the location of tyrosyl and tryptophyl residues in protein. II. Applications of model data to solvent perturbation studies of proteins rich in both tyrosine and tryptophan.
Topics: Animals; Fructose-Bisphosphate Aldolase; Hydrogen-Ion Concentration; Indicators and Reagents; Muscles; Pepsin A; Protein Denaturation; Rabbits; Serum Albumin, Bovine; Spectrophotometry; Tryptophan; Tyrosine; Urea | 1968 |
Subunit structure of the B component of Escherichia coli tryptophan synthetase.
Topics: Amino Acids; Bacterial Proteins; Boron Compounds; Chemical Phenomena; Chemistry; Electrophoresis; Escherichia coli; Formates; Hydro-Lyases; Hydrogen Peroxide; Molecular Weight; Oxidation-Reduction; Peptides; Pyridoxal Phosphate; Sulfhydryl Compounds; Tryptophan; Ultracentrifugation; Urea | 1969 |
Changes in ultraviolet absorption produced by alteration of protein conformation.
Topics: Amides; Amino Acids; Chemical Phenomena; Chemistry; Cystine; Fructose-Bisphosphate Aldolase; Glycols; Histidine; Hydrogen-Ion Concentration; Indoles; Mucoproteins; Muscles; Pepsin A; Phenols; Phenylalanine; Protein Denaturation; Proteins; Spectrum Analysis; Tryptophan; Ultraviolet Rays; Urea; Water | 1969 |
The formation and properties of dimers of the tryptophan synthetase alpha subunit of Escherichia coli.
Topics: Bacterial Proteins; Binding Sites; Centrifugation, Density Gradient; Chemical Phenomena; Chemistry; Chromatography, Gel; Dextrans; Dialysis; Drug Stability; Escherichia coli; Hot Temperature; Hydro-Lyases; Indoles; Kinetics; Methods; Models, Chemical; Models, Structural; Molecular Weight; Peptides; Tryptophan; Urea | 1969 |
Unusual difference spectra of proteins containing tryptophan. II. Studies with proteins.
Topics: Chemical Phenomena; Chemistry; Chymotrypsin; Enzyme Precursors; Lactoglobulins; Muramidase; Pepsin A; Protein Denaturation; Proteins; Serum Albumin, Bovine; Spectrophotometry; Tryptophan; Ultraviolet Rays; Urea | 1969 |
The synthesis of beta-galactosidase in Escherichia coli in the presence of 7-azatryptophan.
Topics: Bacterial Proteins; Enzyme Induction; Escherichia coli; Galactosidases; Hot Temperature; Membrane Transport Proteins; Trypsin; Tryptophan; Urea | 1969 |
Effect of histidine on the enzyme which catalyzes the first step of histidine biosynthesis in Salmonella typhimurium.
Topics: Chemical Phenomena; Chemistry; Circular Dichroism; Feedback; Fluorescence; Fluorometry; Glycerol; Histidine; Ions; Mutation; Pentosephosphates; Protein Binding; Protein Denaturation; Salmonella typhimurium; Solvents; Spectrophotometry; Spectrum Analysis; Transferases; Tryptophan; Tyrosine; Ultraviolet Rays; Urea | 1971 |
Location of aromatic amino acids and belix content in Escherichia coli ribonucleic acid polymerase.
Topics: Amino Acids; Escherichia coli; Glycerol; Mercaptoethanol; Molecular Biology; Optical Rotatory Dispersion; RNA Nucleotidyltransferases; Spectrum Analysis; Tryptophan; Tyrosine; Urea | 1971 |
L-Asparaginase from Escherichia coli B. Physicochemical studies of the dissociation process.
Topics: Alcohols; Asparaginase; Binding Sites; Detergents; Dioxins; Escherichia coli; Fluorometry; Macromolecular Substances; Protein Binding; Protein Denaturation; Spectrophotometry; Sulfuric Acids; Tryptophan; Tyrosine; Ultracentrifugation; Ultraviolet Rays; Urea | 1971 |
Association-dissociation phenomena in glycinin.
Topics: Chemical Phenomena; Chemistry; Electrophoresis, Disc; Hot Temperature; Hydrogen-Ion Concentration; Immunodiffusion; Mercaptoethanol; Plant Proteins; Protein Denaturation; Spectrum Analysis; Tryptophan; Tyrosine; Ultraviolet Rays; Urea | 1969 |
Effect of calcium ion on S-100, a protein of the nervous system.
Topics: Acrylates; Animals; Antigens; Brain Chemistry; Calcium; Cattle; Chemical Phenomena; Chemistry; Chymotrypsin; Cysteine; Electrophoresis; Fluorometry; Immune Sera; Immunodiffusion; Magnesium; Methods; Nerve Tissue Proteins; Optical Rotatory Dispersion; Phenylalanine; Potassium; Protein Binding; Protein Denaturation; Quaternary Ammonium Compounds; Rabbits; Sodium; Solubility; Spectrophotometry; Strontium; Sulfhydryl Compounds; Time Factors; Tryptophan; Tyrosine; Urea; Viscosity | 1969 |
Equine immunoglobulins: a comparison of molecular properties.
Topics: Amino Acids; Animals; Antibodies; Antigens; Blood Glucose; Chromatography, Gel; Diphtheria Toxin; Electrophoresis; Erythema; Hexoses; Horses; Hydrogen-Ion Concentration; Immune Sera; Immunodiffusion; Immunoelectrophoresis; Immunoglobulin G; Molecular Weight; Optical Rotatory Dispersion; Rabbits; Skin Tests; Spectrophotometry; Tryptophan; Tyrosine; Ultracentrifugation; Ultraviolet Rays; Urea | 1969 |
The role of the tryptophan residues in the activity of the nerve growth factor.
Topics: Animals; Complement Fixation Tests; Growth Substances; Immunodiffusion; Mice; Nerve Growth Factors; Oxidation-Reduction; Pyrrolidinones; Snakes; Spectrum Analysis; Submandibular Gland; Sympathetic Nervous System; Tryptophan; Ultraviolet Rays; Urea; Venoms | 1970 |
[Nitrogen metabolism in parenteral feeding].
Topics: Adult; Aged; Alanine; Amino Acids; Ammonia; Creatinine; Diet Therapy; Duodenal Ulcer; Female; Glycine; Humans; Male; Methionine; Middle Aged; Nitrogen; Parenteral Nutrition; Proline; Serine; Stomach Ulcer; Tryptophan; Urea | 1971 |
Structural investigations on pancreatic alpha-amylase. The effect of urea on the ultraviolet absorption.
Topics: Amylases; Animals; Calcium; Chemical Phenomena; Chemistry; Edetic Acid; Hydrogen-Ion Concentration; Kinetics; Pancreas; Protein Binding; Protein Denaturation; Solvents; Spectrophotometry; Swine; Tryptophan; Tyrosine; Ultraviolet Rays; Urea | 1970 |
Properties and subunit structure of the B component of Pseudomonas putida tryptophan synthetase.
Topics: Alanine; Amino Acids; Borohydrides; Carboxypeptidases; Catalysis; Chemical Precipitation; Chromatography, Gel; Cross Reactions; Detergents; Drug Stability; Electrophoresis, Disc; Escherichia coli; Formates; Glycerophosphates; Hot Temperature; Hydro-Lyases; Immune Sera; Immunodiffusion; Indoles; Kinetics; Macromolecular Substances; Mercaptoethanol; Molecular Weight; Oxidation-Reduction; Pseudomonas; Pyridoxal Phosphate; Serine; Spectrophotometry; Sulfuric Acids; Tryptophan; Ultracentrifugation; Urea | 1971 |
Structural properties of bovine brain S-100 protein.
Topics: Alkylation; Amino Acids; Ammonium Sulfate; Animals; Benzoates; Brain; Calcium; Cattle; Chemical Precipitation; Chromatography, Gel; Chromatography, Ion Exchange; Disulfides; Immune Sera; Immunodiffusion; Iodoacetates; Kinetics; Macromolecular Substances; Magnesium; Maleates; Mercaptoethanol; Molecular Weight; Nerve Tissue Proteins; Nitro Compounds; Protein Conformation; Strontium; Sulfhydryl Compounds; Trypsin; Tryptophan; Ultracentrifugation; Urea | 1971 |
Tryptophanase from Bacillus alvei. I. Subunit structure.
Topics: Amino Acid Sequence; Amino Acids; Apoproteins; Bacillus; Borohydrides; Chromatography, Paper; Electrophoresis; Electrophoresis, Paper; Formates; Guanidines; Hydro-Lyases; Hydrogen-Ion Concentration; Hydroxylamines; Macromolecular Substances; Molecular Weight; Oxidation-Reduction; Peptides; Pyridoxal Phosphate; Sodium Dodecyl Sulfate; Trypsin; Tryptophan; Ultracentrifugation; Urea | 1972 |
Conformational properties of cyanogen bromide-cleaved glucagon.
Topics: Amino Acids; Carboxypeptidases; Circular Dichroism; Cyanogen Bromide; Ethanol; Fluorescence; Glucagon; Hydrogen-Ion Concentration; Infrared Rays; Magnetic Resonance Spectroscopy; Peptides; Protein Conformation; Protons; Spectrometry, Fluorescence; Spectrophotometry; Tryptophan; Ultracentrifugation; Ultraviolet Rays; Urea | 1972 |
Location of tryptophyl and tyrosyl residues in human chorionic gonadotropin.
Topics: Chorionic Gonadotropin; Deuterium; Glycols; Humans; Mercaptoethanol; Protein Conformation; Spectrophotometry; Sucrose; Tryptophan; Tyrosine; Ultraviolet Rays; Urea | 1972 |
The subunit structure of tryptophanyl transfer ribonucleic acid synthetase from beef pancreas.
Topics: Alkylation; Amino Acids; Amino Acyl-tRNA Synthetases; Animals; Carbon Isotopes; Carboxypeptidases; Cattle; Chromatography, Gel; Chromatography, Paper; Cyanogen Bromide; Dansyl Compounds; Electrophoresis; Electrophoresis, Paper; Guanidines; Light; Macromolecular Substances; Molecular Weight; Oxidation-Reduction; Pancreas; Peptides; Protein Denaturation; Scattering, Radiation; Sodium Dodecyl Sulfate; Tritium; Trypsin; Tryptophan; Ultracentrifugation; Urea | 1972 |
Structure-function relationships of neurotoxins isolated from Naja haje venom. Physiochemical properties and identification of the active site.
Topics: Acylation; Amides; Amino Acids; Animals; Binding Sites; Chemical Phenomena; Chemistry; Dansyl Compounds; Hot Temperature; Hydrogen-Ion Concentration; Lysine; Mice; Nitrates; Optical Rotatory Dispersion; Protein Conformation; Protein Denaturation; Snakes; Spectrometry, Fluorescence; Spectrophotometry; Structure-Activity Relationship; Toxins, Biological; Trypsin; Tryptophan; Tyrosine; Ultraviolet Rays; Urea; Venoms | 1972 |
Protein nutritional value of opaque-2 corn grain for human adults.
Topics: Adult; Amino Acids; Crosses, Genetic; Deficiency Diseases; Dietary Proteins; Female; Genetics; Humans; Lysine; Male; Methionine; Nitrogen; Nutritional Physiological Phenomena; Plant Proteins; Proteins; Species Specificity; Tryptophan; Urea; Zea mays | 1972 |
The effect of heme binding on the tryptophan residue and the protein conformation of cytochrome b 5 .
Topics: 1-Propanol; Acetates; Acylation; Anhydrides; Animals; Apoproteins; Cattle; Circular Dichroism; Cytochromes; Ethanol; Heme; Mathematics; Protein Binding; Protein Conformation; Protein Denaturation; Rabbits; Spectrometry, Fluorescence; Spectrophotometry; Tryptophan; Ultracentrifugation; Ultraviolet Rays; Urea | 1972 |
The molecular properties of human growth hormone.
Topics: Amino Acid Sequence; Amino Acids; Animals; Chemical Phenomena; Chemistry; Circular Dichroism; Drug Stability; Growth Hormone; Humans; Hydrogen-Ion Concentration; Peptides; Prolactin; Protein Conformation; Sheep; Spectrometry, Fluorescence; Spectrophotometry; Tryptophan; Tyrosine; Ultracentrifugation; Ultraviolet Rays; Urea | 1972 |
Band-shape analysis and display of fine structure in protein spectra: a new approach to perturbation spectroscopy.
Topics: Acetates; Animals; Apoproteins; Aspartate Aminotransferases; Cattle; Chymotrypsinogen; Computers; Cysteine; Egg White; Ethanol; Glucagon; Mathematics; Methods; Muramidase; Myocardium; Pancreas; Phenylalanine; Protein Conformation; Protein Denaturation; Ribonucleases; Spectrophotometry; Swine; Tryptophan; Tyrosine; Ultraviolet Rays; Urea | 1972 |
The denaturation of muscle phosphorylase b by urea.
Topics: Animals; Circular Dichroism; Electrophoresis, Disc; Glucosyltransferases; Macromolecular Substances; Muscles; Phosphorylases; Protein Conformation; Protein Denaturation; Pyridoxamine; Rabbits; Spectrometry, Fluorescence; Tryptophan; Tyrosine; Ultracentrifugation; Ultraviolet Rays; Urea | 1972 |
Circular dichroism studies on chemically modified derivatives of concanavalin A.
Topics: Bromine; Chemical Phenomena; Chemistry; Circular Dichroism; Concanavalin A; Cyanates; Dioxins; Fluorides; Hydrogen Peroxide; Hydrogen-Ion Concentration; Imidazoles; Lectins; Methane; Nitrogen; Oxidation-Reduction; Ribonucleases; Spectrophotometry, Ultraviolet; Spectrum Analysis; Succinimides; Tryptophan; Tyrosine; Urea | 1972 |
The molecular properties of human chorionic somatomammotropin.
Topics: Animals; Cattle; Chemical Phenomena; Chemistry; Circular Dichroism; Female; Fluorometry; Growth Hormone; Humans; Hydrogen-Ion Concentration; Light; Mathematics; Naphthalenes; Placental Hormones; Placental Lactogen; Pregnancy; Prolactin; Protein Denaturation; Rotation; Sheep; Spectrophotometry; Sulfonic Acids; Tryptophan; Tyrosine; Ultraviolet Rays; Urea | 1971 |
Solute perturbation of protein fluorescence. The quenching of the tryptophyl fluorescence of model compounds and of lysozyme by iodide ion.
Topics: Acetamides; Acetates; Butyrates; Circular Dichroism; Fluorescence; Fluorometry; Glutamates; Guanidines; Hydrochloric Acid; Hydrogen-Ion Concentration; Indoles; Iodides; Kinetics; Lysine; Mathematics; Models, Biological; Muramidase; Optical Rotatory Dispersion; Osmolar Concentration; Peptides; Potassium; Propionates; Protein Denaturation; Skatole; Sodium Chloride; Spectrophotometry; Thermodynamics; Tryptophan; Ultraviolet Rays; Urea | 1971 |
An immunoglobulin light chain of subgroup III-1 of lambda type with a free sulfhydryl group.
Topics: Amino Acid Sequence; Ammonium Sulfate; Bence Jones Protein; Chemical Precipitation; Chromatography, Gel; Cysteine; Dansyl Compounds; Dithiothreitol; Electrophoresis; Guanidines; Humans; Immunoglobulin G; Immunoglobulins; Iodine Isotopes; Iodoacetates; Methylation; Multiple Myeloma; Myeloma Proteins; Oxidation-Reduction; Peptides; Protein Denaturation; Sulfhydryl Compounds; Tryptophan; Tyrosine; Urea; Valine | 1971 |
Permeability of the lens capsule to tryptophan.
Topics: Animals; Cattle; Cell Membrane Permeability; Glycine; Humans; Hydrogen-Ion Concentration; Hypoxanthines; In Vitro Techniques; Inositol; Lens, Crystalline; Leucine; Rabbits; Structure-Activity Relationship; Tryptophan; Urea | 1971 |
Separation and characterization of -gliadin fractions.
Topics: Amino Acids; Chlorides; Chromatography, Gel; Chromatography, Ion Exchange; Electrophoresis; Glutens; Hydrogen-Ion Concentration; Plant Proteins; Spectrophotometry; Sulfhydryl Compounds; Triticum; Tryptophan; Tyrosine; Ultracentrifugation; Ultraviolet Rays; Urea | 1971 |
Studies on crystalline yeast phosphoglucomutase: fundamental properties and chemical modification.
Topics: Amino Acids; Aniline Compounds; Binding Sites; Bromine; Chloromercuribenzoates; Fluorometry; Glucosephosphates; Molecular Weight; Naphthalenes; Oxidation-Reduction; Phosphoglucomutase; Protein Binding; Protein Conformation; Saccharomyces; Spectrophotometry; Succinimides; Sulfonic Acids; Tryptophan; Ultracentrifugation; Ultraviolet Rays; Urea | 1971 |
L-tryptophan. A non-allosteric organ-specific uncompetitive inhibitor of human placental alkaline phosphatase.
Topics: Alkaline Phosphatase; Binding Sites; Bone and Bones; Female; Hot Temperature; Humans; Intestines; Kinetics; Liver; Organ Specificity; Placenta; Pregnancy; Protein Denaturation; Stereoisomerism; Thermodynamics; Tryptophan; Urea | 1971 |
Changes in conformation of insolubilized trypsin and chymotrypsin, followed by fluorescence.
Topics: Alanine; Binding Sites; Carboxylic Acids; Chymotrypsin; Cyanogen Bromide; Hot Temperature; Hydrogen-Ion Concentration; Mathematics; Naphthalenes; Peptides; Polysaccharides; Protein Binding; Protein Conformation; Protein Denaturation; Solubility; Spectrometry, Fluorescence; Sulfonic Acids; Temperature; Toluidines; Trypsin; Trypsin Inhibitors; Tryptophan; Urea | 1971 |
Changes in the exposure of the tyrosyl and tryptophyl residues in trypsin due to diisopropylphosphoryl and benzamidine inhibition.
Topics: Amidines; Benzene Derivatives; Binding Sites; Chemical Phenomena; Chemistry; Circular Dichroism; Dimethyl Sulfoxide; Glycerol; Glycols; Hydrogen-Ion Concentration; Isoflurophate; Mathematics; Protein Conformation; Spectrophotometry; Sucrose; Trypsin; Trypsin Inhibitors; Tryptophan; Tyrosine; Ultraviolet Rays; Urea | 1971 |
[Free amino acids and related compounds in the scrapable horny layer of uninvolved skin in microbial eczema and subjects without skin disease].
Topics: Adolescent; Adult; Aged; Amino Acids; Ammonia; Binding Sites; Chromatography; Chromatography, Paper; Eczema; Epithelium; Female; Glutamates; Histidine; Humans; Isoleucine; Male; Middle Aged; Nitrogen; Skin; Tryptophan; Urea | 1971 |
Exposure of the tyrosyl and tryptophyl residues in trypsin and trypsinogen.
Topics: Animals; Arginine; Benzyl Compounds; Binding Sites; Calcium Chloride; Catalysis; Cattle; Chemical Phenomena; Chemistry; Chromatography, Ion Exchange; Circular Dichroism; Dimethyl Sulfoxide; Formates; Glucose; Glycerol; Glycols; Hydrogen-Ion Concentration; Lysine; Mathematics; Nitrophenols; Optical Rotatory Dispersion; Protein Conformation; Protein Denaturation; Solvents; Spectrophotometry; Sucrose; Sulfonic Acids; Toluene; Trypsin; Trypsinogen; Tryptophan; Tyrosine; Ultraviolet Rays; Urea | 1971 |
Evidence for the binding of pyridoxal 5'-phosphate in a hydrophobic region of glycogen phosphorylase b dimer.
Topics: Animals; Binding Sites; Electrochemistry; Electrophoresis, Disc; Electrophoresis, Paper; Energy Transfer; Fluorescence; Glucosyltransferases; Hydrogen-Ion Concentration; Hydroxylamines; Imidazoles; Muscles; Oxidation-Reduction; Phosphorylases; Polymers; Protein Binding; Pyridoxal Phosphate; Rabbits; Time Factors; Tryptophan; Urea | 1971 |
Spectral peculiarities of the monomer-dimer transition of the phospholipases A 2 of Crotalus adamanteus venom.
Topics: Animals; Chemical Phenomena; Chemistry; Circular Dichroism; Dimethyl Sulfoxide; Glycerol; Hydrogen-Ion Concentration; Macromolecular Substances; Methanol; Phospholipases; Protein Conformation; Snakes; Spectrometry, Fluorescence; Spectrophotometry; Sucrose; Tryptophan; Ultracentrifugation; Ultraviolet Rays; Urea; Venoms | 1971 |
Acidic structural proteins of connective tissue. I. Solubilization and preliminary chemical characterization.
Topics: Age Factors; Amino Acids; Animals; Autoanalysis; Boron Compounds; Carbohydrates; Carbonates; Cattle; Chemical Phenomena; Chemical Precipitation; Chemistry; Connective Tissue; Detergents; Female; Guanidines; Humans; Hydrogen-Ion Concentration; Hydroxyproline; Joints; Ligaments; Methods; Placenta; Pregnancy; Protein Denaturation; Proteins; Rabbits; Rats; Skin; Sodium Hydroxide; Solubility; Species Specificity; Sulfates; Tryptophan; Urea | 1969 |
Studies on crystalline lactate dehydrogenase from cardiac and skeletal muscle of plaice (Pleuronectes platessa) with particular reference to temperature.
Topics: Amino Acids; Animals; Cattle; Chemical Phenomena; Chemistry; Electrophoresis; Feedback; Fishes; Gels; Isoenzymes; Kinetics; L-Lactate Dehydrogenase; Methods; Muscles; Myocardium; Pyruvates; Species Specificity; Starch; Temperature; Tryptophan; Ultracentrifugation; Urea | 1969 |
Macromolecule--small molecule interactions. Strong binding and cooperativity in a model synthetic polymer.
Topics: Binding Sites; Chemical Phenomena; Chemistry; Coloring Agents; Fatty Acids; Imines; Lactoglobulins; Macromolecular Substances; Polyethylenes; Polymers; Serum Albumin; Solubility; Spectrum Analysis; Tryptophan; Tyrosine; Urea | 1969 |
Effect of various environments on the intrinsic fluorescence polarization spectra of horse liver alcohol dehydrogenase.
Topics: Alcohol Oxidoreductases; Animals; Cattle; Chemical Phenomena; Chemistry; Chickens; Electrochemistry; Fluorescence; Fluorometry; Guanidines; Horses; Hydrogen-Ion Concentration; Liver; Methods; Muramidase; Osmolar Concentration; Ovalbumin; Peptides; Protein Denaturation; Serum Albumin, Bovine; Tryptophan; Ultracentrifugation; Urea; Viscosity; Zinc | 1969 |
Titration of malate dehydrogenase with 2-hydroxy-5-nitrobenzyl bromide.
Topics: Animals; Binding Sites; Bromine; Chemical Phenomena; Chemistry; Malate Dehydrogenase; Mitochondria, Muscle; Myocardium; Nitrobenzenes; Protein Denaturation; Spectrum Analysis; Swine; Tryptophan; Ultraviolet Rays; Urea | 1969 |
Evidence from amino acid analysis for a relationship in the biosynthesis of gamma- and beta-caseins.
Topics: Amino Acids; Animals; Arginine; Autoanalysis; Caseins; Cattle; Chemical Precipitation; Chromatography, DEAE-Cellulose; Computers; Electrophoresis, Disc; Glutamates; Hexoses; Histidine; Hydrogen-Ion Concentration; Lysine; Methods; Milk; Molecular Biology; Peptides; Phosphorus; Proline; Serine; Tryptophan; Urea | 1969 |
The oxidation with N-bromosuccinimide of the single tryptophan residue in ribonuclease T1.
Topics: Alkylation; Amino Acids; Aspergillus; Bromine; Chemical Phenomena; Chemistry; Circular Dichroism; Cystine; Electrophoresis; Histidine; Imides; Iodoacetates; Kinetics; Optical Rotatory Dispersion; Oxidation-Reduction; Protein Conformation; Ribonucleases; Solubility; Spectrophotometry; Succinates; Tryptophan; Ultraviolet Rays; Urea | 1969 |
Studies on human somatotropin. Preparation and some physico-chemical properties.
Topics: Amino Acid Sequence; Amino Acids; Ammonium Sulfate; Animals; Biological Assay; Body Weight; Carboxypeptidases; Chemical Phenomena; Chemical Precipitation; Chemistry; Chromatography, Gel; Chromatography, Paper; Disulfides; Electrophoresis; Electrophoresis, Disc; Female; Fluorine; Formates; Freeze Drying; Growth Hormone; Humans; Hydrogen-Ion Concentration; Hypophysectomy; Isoflurophate; Macromolecular Substances; Molecular Weight; Nitrobenzenes; Oxidation-Reduction; Polarography; Polymers; Rats; Spectrophotometry; Tryptophan; Tyrosine; Ultraviolet Rays; Urea | 1969 |
An investigation of the subunit structure of yeast enolase.
Topics: Alanine; Amino Acid Sequence; Arginine; Chromatography; Cyanides; Electrophoresis, Disc; Glycerophosphates; Glycine; Histidine; Hydro-Lyases; Peptides; Spectrum Analysis; Trypsin; Tryptophan; Tyrosine; Urea; Yeasts | 1970 |
Identification of metabolites and their chemical removal from blood in renal failure.
Topics: Charcoal; Chromatography, Gas; Creatinine; Kidneys, Artificial; Membranes, Artificial; Phenylalanine; Tryptophan; Urea; Uremia; Uric Acid | 1970 |
Fluorescence studies on ribonuclease T-1.
Topics: Amino Acids; Chemical Phenomena; Chemistry; Detergents; Dioxins; Fluorescence; Hydrogen-Ion Concentration; Nucleotidyltransferases; Protein Denaturation; Tryptophan; Tyrosine; Urea | 1970 |
[Tryptophan-tRNA synthetase from beef pancreas. Characteristics of its intrinsic and extrinsic chromophores in relation to the conformation of the enzyme and its active site].
Topics: Amino Acids; Animals; Binding Sites; Cattle; Chemical Phenomena; Chemistry; Coloring Agents; Fluorometry; Glycerol; Glycols; Ligases; Methods; Pancreas; Spectrophotometry; Tryptamines; Tryptophan; Tyrosine; Urea | 1970 |
The structure and function of ribonuclease T1. 8. Reaction of 2-hydroxy-5-nitrobenzyl bromide with the single tryptophan residue in ribonuclease T1.
Topics: Amino Acids; Benzene Derivatives; Bromine; Carbon Isotopes; Chemical Phenomena; Chemistry; Chromatography, Paper; Hydrogen-Ion Concentration; Ribonucleases; Spectrophotometry; Tryptophan; Urea | 1970 |
Urea and amino acid supplementation of swine diets.
Topics: Amino Acids; Animals; Blood Urea Nitrogen; Body Weight; Depression, Chemical; Diet; Female; Flour; Glycine max; Hematocrit; Lysine; Male; Metabolism; Methionine; Nitrogen; Swine; Time Factors; Tryptophan; Urea; Zea mays | 1970 |
Selective reaction of tyrosyl side chains with iodine in D-glyceraldehyde 3-phosphate dehydrogenase. 1. Study of the differential formation of monoiodo and diiodo derivatives.
Topics: Animals; Autoradiography; Chemical Phenomena; Chemistry; Diiodotyrosine; Glyceraldehyde-3-Phosphate Dehydrogenases; Iodine; Iodine Isotopes; Monoiodotyrosine; Muscles; Optical Rotatory Dispersion; Peptides; Protein Denaturation; Solvents; Spectrophotometry; Spectrum Analysis; Swine; Time Factors; Trypsin; Tryptophan; Tyrosine; Urea; Viscosity | 1970 |
Effect of level of total nitrogen intake on second limiting amino acid in corn for humans.
Topics: Adult; Amino Acids; Citrates; Diet; Glycine; Humans; Lysine; Male; Metabolism; Methionine; Nitrogen; Quaternary Ammonium Compounds; Tryptophan; Urea; Zea mays | 1970 |
Qualitative assessment of supplemental amino acid needs for growing lambs fed urea as the sole nitrogen source.
Topics: Abomasum; Amino Acids; Analysis of Variance; Animals; Diet; Glutamates; Histidine; Leucine; Lysine; Male; Metabolism; Methionine; Nitrogen; Sheep; Threonine; Tryptophan; Urea | 1970 |
The nerve growth factor. Amino acid composition and physicochemical properties.
Topics: Amino Acids; Animals; Cystine; Growth Substances; Guanidines; Hydrogen-Ion Concentration; Isoelectric Focusing; Mice; Nerve Growth Factors; Nerve Tissue; Nitrogen; Oxidation-Reduction; Solubility; Spectrophotometry; Submandibular Gland; Sulfhydryl Compounds; Tryptophan; Ultraviolet Rays; Urea | 1970 |
Selective reaction of tyrosyl side chains with iodine in glyceraldehyde-3-phosphate dehydrogenase. 3. Specificity of the reaction in urea. Determination of reacting histidine and tryptophan (short communication).
Topics: Chemical Phenomena; Chemistry; Glyceraldehyde-3-Phosphate Dehydrogenases; Histidine; Iodine; Iodine Isotopes; Pyrrolidinones; Spectrophotometry; Tryptophan; Tyrosine; Urea | 1970 |
[Metabolism of the isolated perfused rat liver after in vitro irradiation. II. Intracellular and extracellular contents of free amino acids, taurine and urea].
Topics: Amino Acids; Animals; Arginine; Biological Transport; Cytoplasm; In Vitro Techniques; Liver; Radiation Effects; Rats; Taurine; Threonine; Tryptophan; Urea | 1970 |
On the structural stability and solvent denaturation of proteins. 3. Denaturation by the amides.
Topics: Alcohols; Amides; Animals; Cattle; Cetacea; Chymotrypsin; Cytochromes; Enzyme Precursors; Esters; Formamides; Glycols; Horses; Hydrogen-Ion Concentration; Indoles; Mathematics; Myocardium; Myoglobin; Optical Rotatory Dispersion; Protein Binding; Protein Denaturation; Solubility; Solvents; Spectrophotometry; Tryptophan; Urea | 1970 |
Reaction of diethyl pyrocarbonate with indole derivatives with special reference to the reaction with tryptophan residues in a protein.
Topics: Animals; Carbonates; Cattle; Chemical Phenomena; Chemistry; Chromatography, Gas; Chromatography, Gel; Fluorescence; Indoles; Serum Albumin, Bovine; Spectrum Analysis; Tryptophan; Urea | 1970 |
The properties of bovine growth hormone. IV. Circular dichroism of native and iodinated molecules.
Topics: Animals; Carbonates; Cattle; Chemical Phenomena; Chemistry; Circular Dichroism; Growth Hormone; Hydrogen-Ion Concentration; Iodine; Models, Chemical; Phenylalanine; Piperazines; Tryptophan; Tyrosine; Ultraviolet Rays; Urea | 1970 |
Spectroscopic studies on the conformation of native and denatured glycinin.
Topics: Binding Sites; Glycine max; Guanidines; Hydrogen-Ion Concentration; Optical Rotatory Dispersion; Plant Proteins; Protein Binding; Protein Conformation; Protein Denaturation; Solvents; Spectrophotometry, Infrared; Spectrophotometry, Ultraviolet; Tryptophan; Urea | 1970 |
States of tyrosyl and tryptophyl residues in Bence Jones proteins.
Topics: Bence Jones Protein; Bromides; Chemical Phenomena; Chemistry; Imidazoles; Nitrobenzenes; Protein Denaturation; Spectrophotometry; Tryptophan; Tyrosine; Ultraviolet Rays; Urea | 1971 |
Amino acid metabolism in the isolated perfused rat liver.
Topics: Alanine; Amino Acids; Ammonia; Animals; Aspartic Acid; Chromatography; Glutamates; Glycine; In Vitro Techniques; Liver; Lysine; Male; Methionine; Nitrogen Isotopes; Perfusion; Phenylalanine; Proline; Rats; Serine; Threonine; Tryptophan; Tyrosine; Urea | 1971 |
Membrane structure: the reactivity of tryptophan, tyrosine and lysine in proteins of the microsomal structure.
Topics: Alkanes; Amino Acids; Animals; Carbamates; Carbohydrates; Cell Fractionation; Centrifugation, Density Gradient; Chemical Phenomena; Chemistry; Coliphages; Cyanates; Depression, Chemical; Detergents; Lipids; Lysine; Membranes; Microsomes, Liver; Nitro Compounds; Oxidation-Reduction; Pancreas; Peptides; Proteins; Pyrrolidinones; Rats; Ribonucleases; Stimulation, Chemical; Sulfuric Acids; Time Factors; Tryptophan; Tyrosine; Urea | 1971 |
Circular dichroism studies on the conformation and interaction of T 1 ribonuclease.
Topics: Aspergillus; Binding Sites; Chemical Precipitation; Chromatography, DEAE-Cellulose; Chromatography, Gel; Circular Dichroism; Guanine Nucleotides; Hydrogen-Ion Concentration; Nucleotides; Quaternary Ammonium Compounds; Ribonucleases; Spectrophotometry; Sulfates; Temperature; Tryptophan; Tyrosine; Ultraviolet Rays; Urea | 1971 |
Alcohol-induced depression of albumin synthesis: reversal by tryptophan.
Topics: Animals; Arginine; Carbon Dioxide; Carbon Isotopes; Depression, Chemical; DNA; Drug Antagonism; Ethanol; In Vitro Techniques; Liver; Male; Perfusion; Rabbits; Ribosomes; RNA; Serum Albumin; Tryptophan; Urea | 1971 |
Studies of the mechanism of binding of chemically modified cytophilic antibodies to macrophages.
Topics: Amidines; Animals; Antibodies; Antigen-Antibody Reactions; Antigens; Benzyl Compounds; Binding Sites; Carbamates; Complement Fixation Tests; Complement System Proteins; Cyanates; Erythrocytes; Guinea Pigs; Immunochemistry; Immunoglobulin G; Lysine; Macrophages; Nitrobenzenes; Potassium; Rabbits; Sheep; Tryptophan; Urea | 1971 |
The effect of the administration of L-tryptophan on synthesis of urea and gluconeogenesis in man.
Topics: Acetoacetates; Adult; Amino Acids; Aspartic Acid; Carboxy-Lyases; Female; Gluconeogenesis; Glutamates; Humans; Hydroxybutyrates; Ketoglutaric Acids; Lactates; Liver; Male; Pyruvates; Tryptophan; Urea | 1971 |
Denaturation of proteins in 8M urea as monitored by tryptophan fluorescence: trypsin, trypsinogen and some derivatives.
Topics: Fluorescence; Fluorometry; Hydrogen-Ion Concentration; Isoflurophate; Protein Denaturation; Trypsin; Trypsinogen; Tryptophan; Urea | 1968 |
Studies of the location of tyrosyl and tryptophyl residues in proteins. I. Solvent perturbation data of model compounds.
Topics: Deuterium; Erythritol; Glucose; Glycerol; Glycols; Indicators and Reagents; Methanol; Muramidase; Protein Denaturation; Proteins; Ribonucleases; Spectrophotometry; Sucrose; Sulfoxides; Tryptophan; Tyrosine; Urea | 1968 |
A study of 2,4,6-trinitrobenzenesulfonic acid for automated amino acid chromatography.
Topics: Alanine; Amino Acids; Ammonia; Arginine; Aspartic Acid; Autoanalysis; Chromatography; Cystine; Glycine; Histidine; Hydrogen-Ion Concentration; Indicators and Reagents; Isoleucine; Leucine; Lysine; Methionine; Methods; Nitrobenzenes; Phenylalanine; Proline; Serine; Spectrophotometry; Sulfonic Acids; Temperature; Threonine; Tryptophan; Tyrosine; Urea; Valine | 1968 |
Fluorescence and protein structure. XV. Tryptophan fluorescence in helical muscle protein.
Topics: Detergents; Dipeptides; Fluorescence; Glycine; Hydrogen-Ion Concentration; Models, Chemical; Muscle Proteins; Protein Denaturation; Spectrum Analysis; Sulfates; Tryptophan; Tyrosine; Urea | 1968 |
An analysis of perturbations in the ultraviolet absorption spectra of proteins and model compounds.
Topics: 1-Propanol; Chymotrypsin; Dioxins; Esters; Ethanol; Glycols; Muramidase; Protein Denaturation; Proteins; Serum Albumin; Serum Albumin, Bovine; Solvents; Spectrophotometry; Tryptophan; Tyrosine; Urea | 1968 |
Steroid-protein interactions studies by fluorescence quenching.
Topics: 17-Ketosteroids; Amino Acids; Androstanes; Animals; Cattle; Chemical Phenomena; Chemistry; Fluorescence; Fluorometry; Gelatin; Hydrogen-Ion Concentration; Proteins; Serum Albumin, Bovine; Testosterone; Tryptophan; Tyrosine; Urea | 1968 |
Physicochemical studies of bovine fibrinogen. IV. Ultraviolet absorption and its relation to the structure of the molecule.
Topics: Animals; Cattle; Chemical Phenomena; Chemistry; Fibrinogen; Glycols; Guanidines; Hydrogen-Ion Concentration; Protein Denaturation; Solvents; Spectrophotometry; Tryptophan; Tyrosine; Ultraviolet Rays; Urea | 1968 |
Purification and characterization of a multienzyme complex in the tryptophan pathway of Neurospora crassa.
Topics: Amino Acids; Chloromercuribenzoates; Chromatography, Gel; Chromatography, Ion Exchange; Electrophoresis, Disc; Glycerophosphates; Isomerases; Ligases; Neurospora; ortho-Aminobenzoates; Tryptophan; Ultracentrifugation; Urea | 1969 |
Subunit structure of rabbit muscle pyruvate kinase.
Topics: Acetates; Amino Acids; Animals; Arginine; Electrophoresis, Disc; Guanidines; Hydrazines; Molecular Weight; Muscles; Peptides; Pyruvate Kinase; Rabbits; Trypsin; Tryptophan; Ultracentrifugation; Urea | 1969 |
Action of alpha-methyl-DL-tryptophan in vivo on catabolism of amino acids and their conversion to liver glycogen.
Topics: Acetates; Adrenal Glands; Adrenalectomy; Alanine; Amino Acids; Animals; Body Temperature; Carbon Dioxide; Carbon Isotopes; Glutamates; Histidine; Isoleucine; Leucine; Liver; Liver Glycogen; Male; Pyruvates; Rats; Threonine; Time Factors; Tryptophan; Tryptophan Oxygenase; Urea | 1969 |
Reactivity of tyrosine and tryptophan residues in haptoglobin.
Topics: Alkylation; Animals; Bromides; Chemical Phenomena; Chemistry; Dogs; Haptoglobins; Hydrogen-Ion Concentration; Imidazoles; Protein Denaturation; Spectrophotometry; Spectrum Analysis; Tryptophan; Tyrosine; Ultraviolet Rays; Urea | 1969 |
Solvent perturbation studies of heme proteins and other colored proteins.
Topics: Animals; Catalase; Cattle; Cetacea; Cytochromes; Deuterium; Glycerol; Glycols; Heme; Hemoglobins; Horses; Hydrogen-Ion Concentration; Liver; Myoglobin; Optical Rotatory Dispersion; Solvents; Spectrum Analysis; Sucrose; Tryptophan; Tyrosine; Ultraviolet Rays; Urea; Water | 1969 |
Solvent perturbation studies of heme proteins and other colored proteins. II. On the environment and location of the tryptophyl residues in hemoglobin.
Topics: Animals; Cattle; Dogs; Glycerol; Glycols; Hemoglobins; Horses; Humans; Muramidase; Optical Rotatory Dispersion; Rats; Ribonucleases; Solvents; Spectrum Analysis; Sucrose; Swine; Tryptophan; Ultraviolet Rays; Urea | 1969 |
Low-protein diet and psoriasis. A hospital study.
Topics: Adult; Aged; Blood Urea Nitrogen; Diet Therapy; Dietary Proteins; Female; Humans; Male; Middle Aged; Psoriasis; Taurine; Triamcinolone; Tryptophan; Urea | 1969 |
Amino acid regulation of albumin synthesis.
Topics: Albumins; Amino Acids; Animals; Arginine; Carbon Isotopes; Electrophoresis; Fasting; Immunoelectrophoresis; In Vitro Techniques; Isoleucine; Leucine; Liver; Lysine; Methionine; Methods; Perfusion; Rabbits; Ribosomes; Threonine; Tryptophan; Urea; Valine | 1969 |
Tryptophanyl transfer ribonucleic acid synthetase from bovine pancreas. II. The chemically different subunits.
Topics: Amino Acids; Animals; Boron; Carbon Isotopes; Cattle; Chromatography, Gel; Chromatography, Ion Exchange; Detergents; Dialysis; Guanidines; Iodoacetates; Ligases; Mercaptoethanol; Microscopy, Electron; Molecular Weight; Nitrobenzenes; Pancreas; Peptides; RNA, Transfer; Sulfates; Sulfhydryl Compounds; Trypsin; Tryptophan; Ultracentrifugation; Urea | 1969 |
A comparison of the effects of ultraviolet and ionizing radiations on trypsin activity and on its constituent amino acids.
Topics: Amino Acids; Cobalt Isotopes; Cystine; Electrons; Energy Transfer; Histidine; Methionine; Radiation Effects; Radiometry; Trypsin; Tryptophan; Tyrosine; Ultraviolet Rays; Urea | 1969 |
Conformational states of native and denatured phosphoglucose isomerase. II. Ultraviolet difference spectroscopy.
Topics: Amino Acids; Animals; Chemical Phenomena; Chemistry; Detergents; Glucose-6-Phosphate Isomerase; Glycols; Histidine; Hydrogen-Ion Concentration; Muscles; Protein Denaturation; Rabbits; Spectrophotometry; Sulfates; Sulfhydryl Compounds; Temperature; Tryptophan; Tyrosine; Ultraviolet Rays; Urea | 1969 |
Unusual difference spectra of proteins containing tryptophan. I. Studies with model compounds.
Topics: Alcohols; Animals; Cattle; Chemical Phenomena; Chemistry; Detergents; Dimethyl Sulfoxide; Dioxins; Female; Glycols; Homozygote; Hydrogen-Ion Concentration; Indoles; Lactoglobulins; Milk; Osmolar Concentration; Protein Denaturation; Proteins; Solvents; Spectrophotometry; Temperature; Tryptophan; Ultraviolet Rays; Urea | 1969 |
Beef pancreas tryptophanyl-tRNA synthetase. Molecular weight, composition and spectral properties.
Topics: Amino Acids; Animals; Boron Compounds; Cattle; Chromatography, Gel; Cysteine; Cystine; Drug Stability; Formates; Ligases; Light; Methylation; Molecular Weight; Optical Rotatory Dispersion; Oxidation-Reduction; Pancreas; RNA, Transfer; Spectrophotometry; Spectrum Analysis; Tryptophan; Ultracentrifugation; Ultraviolet Rays; Urea | 1969 |
Structure of lysozyme. X. On the structural role of tryptophan residues.
Topics: Chemical Phenomena; Chemistry; Muramidase; Pyrrolidinones; Tryptophan; Urea | 1965 |
The properties of bovine growth hormone. II. Effects of urea.
Topics: Animals; Cattle; Chemical Phenomena; Chemistry; Fluorescence; Growth Hormone; Hydrogen-Ion Concentration; In Vitro Techniques; Naphthalenes; Tryptophan; Urea | 1966 |
The binding of indole compounds to bovine plasma albumin. Effects of potassium chloride, urea, dioxane, and glycine.
Topics: Chemical Phenomena; Chemistry, Physical; Dioxins; Glycine; Hydrogen-Ion Concentration; Indoles; Potassium Chloride; Serum Albumin, Bovine; Skatole; Solutions; Thermodynamics; Tryptophan; Urea | 1966 |
The properties of bovine growth hormone. 3. Behavior in alkaline solution.
Topics: Animals; Cattle; Chemical Phenomena; Chemistry, Physical; Fluorescence; Growth Hormone; Hydrogen-Ion Concentration; Molecular Weight; Solutions; Tryptophan; Tyrosine; Ultracentrifugation; Urea | 1966 |
Accessibility and structural function of particular amino acid residues of soy bean trypsin inhibition.
Topics: Chemical Phenomena; Chemistry; Cystine; Electrophoresis; Glycine max; Guanidines; Histidine; Hydrogen Peroxide; Lysine; Pyrrolidinones; Trypsin Inhibitors; Tryptophan; Tyrosine; Urea | 1966 |
Tryptophan metabolism in normal and phenylketonuric rats.
Topics: Animals; Carbon Dioxide; Carbon Isotopes; Humans; Indoleacetic Acids; Injections, Intravenous; Kynurenine; Male; Phenylalanine; Phenylketonurias; Rats; Tryptophan; Urea | 1966 |
Contribution of tryptophan to the biologic properties of anti-dinitrophenyl antibody.
Topics: Animals; Antibodies; Antigen-Antibody Reactions; Bromides; Complement Fixation Tests; Dinitrophenols; Fluorescence; Hemagglutination; Passive Cutaneous Anaphylaxis; Rabbits; Tryptophan; Urea | 1967 |
Unfolding reactions of proteins. II. Spectral and optical rotatory dispersion studies in urea, guanidinium chloride, and 2-chloroethanol.
Topics: Chemical Phenomena; Chemistry, Physical; Chlorides; Chlorine; Chymotrypsin; Guanidines; Ions; Kinetics; Muramidase; Protein Denaturation; Solvents; Spectrophotometry; Temperature; Thermodynamics; Tryptophan; Urea | 1967 |
Fluorometric and spectrophotometric study of heme binding on the apoprotein from a cytochrome b-2-derivative.
Topics: Coenzymes; Cytochromes; Flavins; Fluorometry; Heme; L-Lactate Dehydrogenase; Protein Binding; Spectrophotometry; Trypsin; Tryptophan; Tyrosine; Urea | 1967 |
Fluorescence studies of the molecular conformation of alpha-lactalbumin.
Topics: Albumins; Chemical Phenomena; Chemistry, Physical; Fluorescence; Hydrogen-Ion Concentration; Protein Denaturation; Spectrophotometry; Tryptophan; Urea | 1967 |
Effects of pH and some agents on the fluorescence of myosin A.
Topics: Animals; Chlorides; Dioxins; Ethanol; Fluorescence; Fluorescent Dyes; Guanidines; Hydrogen-Ion Concentration; In Vitro Techniques; Muscle Proteins; Rabbits; Spectrophotometry; Tryptophan; Urea | 1967 |
Assessment of nutritional status of men: protein.
Topics: 17-Ketosteroids; Adult; Amino Acids; Body Height; Body Weight; Creatinine; Diet; Humans; Lysine; Male; Methionine; Nitrogen; Protein Deficiency; Tryptophan; Urea | 1967 |
[Problems of protein supply in patients with liver cirrhosis].
Topics: Ammonia; Hepatic Encephalopathy; Humans; Liver Cirrhosis; Methionine; Neurotransmitter Agents; Parenteral Nutrition; Phenylalanine; Proteins; Tryptophan; Tyrosine; Urea | 1982 |
[Luminescent properties of NADPH: adrenodoxin reductase].
Topics: Acrylamide; Acrylamides; Adrenal Cortex; Animals; Apoenzymes; Ferredoxin-NADP Reductase; Flavin-Adenine Dinucleotide; Fluorescence; Luminescent Measurements; Mitochondria; NADH, NADPH Oxidoreductases; Protein Denaturation; Temperature; Tryptophan; Urea | 1984 |
Effects of pH and urea on the conformational properties of subtilisin DY.
Topics: Bacillus subtilis; Hydrogen-Ion Concentration; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Subtilisins; Time Factors; Tryptophan; Tyrosine; Urea | 1982 |
Fluorescence quenching as an indicator for the exposure of tryptophyl residues in Streptomyces subtilisin inhibitor.
Topics: Bacterial Proteins; Cesium; Kinetics; Protein Conformation; Spectrometry, Fluorescence; Streptomyces; Subtilisins; Temperature; Tryptophan; Urea | 1980 |
Intrinsic fluorescence spectra of a tryptophan-containing parvalbumin as a function of thermal, pH and urea denaturation.
Topics: Animals; Calcium; Fishes; Hydrogen-Ion Concentration; Kinetics; Muscle Proteins; Parvalbumins; Protein Denaturation; Spectrometry, Fluorescence; Temperature; Tryptophan; Urea | 1982 |
Fluorescence spectra of lysozyme excited at 305 NM in presence of urea.
Topics: Animals; Chickens; Hydrogen-Ion Concentration; Muramidase; Protein Conformation; Spectrometry, Fluorescence; Tryptophan; Urea | 1981 |
The role of the cysteinyl and one of the tryptophyl residues in the neurotoxic action of suberitine.
Topics: Cysteine; Gentian Violet; Light; Marine Toxins; Neurotoxins; Proflavine; Protein Denaturation; Proteins; Structure-Activity Relationship; Tryptophan; Urea | 1981 |
Conformational differences between high clotting human alpha-thrombin and nonclotting gamma-thrombin.
Topics: Circular Dichroism; Humans; Indicators and Reagents; Phenylmethylsulfonyl Fluoride; Protein Binding; Protein Conformation; Protein Denaturation; Spectrophotometry, Ultraviolet; Sulfonium Compounds; Thrombin; Tryptophan; Tyrosine; Urea | 1981 |
Optically detected magnetic resonance of tryptophan triplet states in native and urea-denatured proteins and polypeptides.
Topics: Cations, Divalent; Luminescent Measurements; Magnetic Resonance Spectroscopy; Peptides; Protein Binding; Protein Conformation; Protein Denaturation; Proteins; Tryptophan; Urea | 1980 |
Conformational changes of Carcinus maenas haemocyanin induced by urea.
Topics: Animals; Binding Sites; Brachyura; Circular Dichroism; Copper; Hemocyanins; Protein Binding; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Tryptophan; Tyrosine; Urea | 1980 |
The in situ labeling of histone H3 in chromatin by a fluorescent probe.
Topics: Animals; Chromatin; Fluorescent Dyes; Histones; Hydrogen-Ion Concentration; Kinetics; Liver; Maleimides; Mice; Osmolar Concentration; Pyrenes; Rats; Sodium Dodecyl Sulfate; Spectrometry, Fluorescence; Tryptophan; Urea | 1980 |
Unfolding properties of tryptophan-containing alpha-subunits of the Escherichia coli tryptophan synthase.
Topics: Calorimetry; Escherichia coli; Kinetics; Luminescent Measurements; Macromolecular Substances; Models, Structural; Mutagenesis, Site-Directed; Point Mutation; Protein Denaturation; Protein Folding; Recombinant Proteins; Spectrometry, Fluorescence; Tryptophan; Tryptophan Synthase; Urea | 1995 |
Tryptophan-containing alpha-subunits of the Escherichia coli tryptophan synthase. Enzymatic and urea stability properties.
Topics: Base Sequence; Enzyme Stability; Escherichia coli; Molecular Sequence Data; Oligodeoxyribonucleotides; Protein Folding; Thermodynamics; Tryptophan; Tryptophan Synthase; Urea | 1995 |
Effects of protein source on nitrogen metabolism in continuous culture and intestinal digestion in vitro.
Topics: Amino Acids; Animals; Blood Proteins; Cattle; Dietary Proteins; Digestion; Fatty Acids, Volatile; Female; Fermentation; Fish Products; Glycine max; Intestinal Mucosa; Intestines; Nitrogen; Tryptophan; Urea; Zea mays | 1995 |
Comparing the refolding and reoxidation of recombinant porcine growth hormone from a urea denatured state and from Escherichia coli inclusion bodies.
Topics: Animals; Disulfides; Drug Stability; Escherichia coli; Growth Hormone; Inclusion Bodies; Mercaptoethanol; Oxidation-Reduction; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Spectrometry, Fluorescence; Swine; Thermodynamics; Tryptophan; Urea | 1995 |
Interdomain communication of T-cell CD4 studied by absorbance and fluorescence difference spectroscopy measurements of urea-induced unfolding.
Topics: CD4 Antigens; Humans; In Vitro Techniques; Peptide Fragments; Protein Denaturation; Solubility; Spectrometry, Fluorescence; T-Lymphocytes; Thermodynamics; Tryptophan; Urea | 1995 |
The refolding of hen egg white riboflavin binding protein.
Topics: Animals; Carrier Proteins; Chickens; Dithiothreitol; Edetic Acid; Female; Guanidine; Guanidines; Membrane Transport Proteins; Phosphorylation; Protein Conformation; Protein Denaturation; Protein Folding; Riboflavin; Spectrometry, Fluorescence; Tryptophan; Urea | 1995 |
Evidence for residual structures in an unfolded form of yeast phosphoglycerate kinase.
Topics: Guanidine; Guanidines; Mutagenesis, Site-Directed; Phenylalanine; Phosphoglycerate Kinase; Protein Conformation; Protein Folding; Saccharomyces cerevisiae; Spectrometry, Fluorescence; Tryptophan; Tyrosine; Urea | 1995 |
Denaturation behavior of alpha-chymotrypsinogen A in urea and alkylurea solutions: fluorescence studies.
Topics: Animals; Cattle; Chemical Phenomena; Chemistry, Physical; Chromatography; Chymotrypsinogen; Drug Stability; Fluorescence Polarization; Fluorometry; Hydrogen-Ion Concentration; Methylurea Compounds; Protein Denaturation; Protein Folding; Protein Precursors; Solutions; Spectrometry, Fluorescence; Tryptophan; Urea; Water | 1994 |
Local unfolding and the stepwise loss of the functional properties of tubulin.
Topics: Anilino Naphthalenesulfonates; Animals; Brain Chemistry; Chymotrypsin; Colchicine; Fluorescent Dyes; GTP Phosphohydrolases; Kinetics; Microtubules; Polymers; Protein Folding; Rats; Spectrometry, Fluorescence; Tropolone; Trypsin; Tryptophan; Tubulin; Urea | 1994 |
Characterization of binding and structural properties of rat liver fatty-acid-binding protein using tryptophan mutants.
Topics: Animals; Carrier Proteins; Energy Transfer; Fatty Acid-Binding Protein 7; Fatty Acid-Binding Proteins; Fatty Acids; Fluorescent Dyes; Liver; Mutagenesis, Site-Directed; Neoplasm Proteins; Nerve Tissue Proteins; Protein Denaturation; Rats; Spectrometry, Fluorescence; Structure-Activity Relationship; Tryptophan; Urea | 1994 |
Arg-188 and Trp-144 are implicated in the binding of urea and acetamide to the active site of the amidase from Pseudomonas aeruginosa.
Topics: Acetamides; Amidohydrolases; Arginine; Base Sequence; Binding Sites; Dithionitrobenzoic Acid; Enzyme Stability; Hot Temperature; Molecular Sequence Data; Mutation; Polymerase Chain Reaction; Pseudomonas aeruginosa; Tryptophan; Urea | 1994 |
19F NMR spectroscopy of [6-19F]tryptophan-labeled Escherichia coli dihydrofolate reductase: equilibrium folding and ligand binding studies.
Topics: Base Sequence; Binding Sites; DNA Primers; Escherichia coli; Ligands; Magnetic Resonance Spectroscopy; Methotrexate; Molecular Sequence Data; Mutagenesis, Site-Directed; NADP; Protein Conformation; Protein Folding; Tetrahydrofolate Dehydrogenase; Tryptophan; Urea | 1994 |
Tryptophan phosphorescence as a structural probe of mitochondrial F1-ATPase epsilon-subunit.
Topics: Adenosine Triphosphate; Animals; Cattle; Cold Temperature; Guanidine; Guanidines; Kinetics; Luminescent Measurements; Mitochondria, Heart; Nucleotides; Proton-Translocating ATPases; Spectrophotometry; Tryptophan; Urea | 1993 |
Circular dichroism studies of barnase and its mutants: characterization of the contribution of aromatic side chains.
Topics: Bacterial Proteins; Circular Dichroism; Histidine; Hydrogen-Ion Concentration; Molecular Structure; Mutagenesis, Site-Directed; Protein Denaturation; Protein Structure, Secondary; Ribonucleases; Tryptophan; Tyrosine; Urea | 1993 |
Studies on the location of aromatic amino acids in alpha-crystallin.
Topics: Acrylamide; Acrylamides; Amino Acids; Animals; Cattle; Crystallins; Hydrogen-Ion Concentration; Iodides; Protein Conformation; Spectrometry, Fluorescence; Temperature; Tryptophan; Urea | 1993 |
The energetics and cooperativity of protein folding: a simple experimental analysis based upon the solvation of internal residues.
Topics: Algorithms; Amino Acid Sequence; Calorimetry; Geobacillus stearothermophilus; Guanidine; Guanidines; Kinetics; Mathematics; Micrococcal Nuclease; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Phosphoglycerate Kinase; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Software; Staphylococcus aureus; Thermodynamics; Tryptophan; Urea | 1993 |
How to measure and predict the molar absorption coefficient of a protein.
Topics: 1-Propanol; Chemical Phenomena; Chemistry, Physical; Cystine; Guanidine; Guanidines; Proteins; Solvents; Spectrophotometry, Ultraviolet; Tryptophan; Tyrosine; Urea; Water | 1995 |
Effect of feeding reduced protein, amino acid-supplemented diets on nitrogen and energy balance in grower pigs.
Topics: Amino Acids; Ammonia; Animals; Blood Urea Nitrogen; Diet, Protein-Restricted; Energy Metabolism; Food, Fortified; Lysine; Male; Nitrogen; Random Allocation; Swine; Threonine; Tryptophan; Urea | 1995 |
Fatty acid binding and conformational stability of mutants of human muscle fatty acid-binding protein.
Topics: Base Sequence; Binding Sites; Carrier Proteins; DNA Primers; Fatty Acid-Binding Protein 7; Fatty Acid-Binding Proteins; Fatty Acids; Fluorescence; Humans; Ligands; Molecular Sequence Data; Muscles; Mutagenesis, Site-Directed; Myelin P2 Protein; Neoplasm Proteins; Oleic Acid; Oleic Acids; Point Mutation; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Tryptophan; Tumor Suppressor Proteins; Urea | 1996 |
Covalent reinforcement of a fragile region in the dimeric enzyme thymidylate synthase stabilizes the protein against chaotrope-induced unfolding.
Topics: Binding Sites; Chromatography, Gel; Circular Dichroism; Fluorescence; Guanidine; Guanidines; Lacticaseibacillus casei; Models, Chemical; Models, Molecular; Molecular Probes; Mutagenesis, Site-Directed; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins; Thymidylate Synthase; Tryptophan; Urea | 1996 |
Intrinsic tryptophans of CRABPI as probes of structure and folding.
Topics: Circular Dichroism; Fluorescence; Gene Expression; Point Mutation; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Receptors, Retinoic Acid; Recombinant Proteins; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Titrimetry; Tretinoin; Tryptophan; Urea | 1996 |
Folding and stability of endoglucanase III, a single-domain cellulase from Trichoderma reesei.
Topics: Bacterial Proteins; Cellulase; Circular Dichroism; Disulfides; Dithiothreitol; Enzyme Stability; Hydrogen-Ion Concentration; Kinetics; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Spectrometry, Fluorescence; Temperature; Thermodynamics; Trichoderma; Tryptophan; Urea | 1996 |
Disulfide stabilization of antibody Fv: computer predictions and experimental evaluation.
Topics: Amino Acid Sequence; Base Sequence; Cell Survival; Computer Simulation; Disulfides; DNA Primers; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Fluorescence; Immunoglobulin Fragments; Immunoglobulin Variable Region; Immunotoxins; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Denaturation; Protein Engineering; Recombinant Fusion Proteins; Tryptophan; Urea | 1995 |
Bioavailability of tryptophan in soybean meal for 10-kg pigs using slope-ratio assay.
Topics: Amino Acids; Analysis of Variance; Animals; Biological Availability; Diet; Female; Glycine max; Male; Swine; Tryptophan; Urea; Weight Gain | 1996 |
Urea-induced equilibrium unfolding of single tryptophan mutants of yeast phosphoglycerate kinase: evidence for a stable intermediate.
Topics: Circular Dichroism; Guanidine; Guanidines; Kinetics; Phosphoglycerate Kinase; Point Mutation; Protein Denaturation; Protein Folding; Saccharomyces cerevisiae; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Urea | 1996 |
Characterization of charge change super-repressor mutants of trp repressor: effects on oligomerization conformation, ligation and stability.
Topics: Anilino Naphthalenesulfonates; Bacterial Proteins; Circular Dichroism; DNA, Bacterial; Escherichia coli; Fluorescent Dyes; Kinetics; Ligands; Models, Molecular; Mutation; Protein Binding; Protein Conformation; Protein Denaturation; Protein Structure, Secondary; Repressor Proteins; Thermodynamics; Tryptophan; Urea | 1996 |
A lysine 73-->histidine variant of yeast iso-1-cytochrome c: evidence for a native-like intermediate in the unfolding pathway and implications for m value effects.
Topics: Circular Dichroism; Cytochrome c Group; Cytochromes c; Fluorescence; Fungal Proteins; Guanidine; Guanidines; Hydrogen-Ion Concentration; Models, Molecular; Mutagenesis, Site-Directed; Point Mutation; Protein Conformation; Protein Denaturation; Protein Folding; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Spectrophotometry; Spectroscopy, Fourier Transform Infrared; Thermodynamics; Tryptophan; Urea | 1997 |
Tryptophan needs of lactating sows fed diets supplemented with crystalline lysine.
Topics: Amino Acids, Essential; Animals; Crystallization; Diet; Female; Food, Fortified; Lactation; Litter Size; Lysine; Swine; Time Factors; Tryptophan; Urea; Weight Loss | 1997 |
Free amino acid supplementation to steers: effects on ruminal fermentation and performance.
Topics: Amino Acids; Animal Nutritional Physiological Phenomena; Animals; Body Composition; Cattle; Diet; Digestion; Edible Grain; Fermentation; Food, Fortified; Ionophores; Lysine; Male; Methionine; Nitrogen; Rumen; Threonine; Tryptophan; Urea | 1997 |
Fluorescence properties of a tryptophan residue in an aromatic core of the protein subunit of ribonuclease P from Escherichia coli.
Topics: Bacterial Proteins; Endoribonucleases; Enzyme Inhibitors; Escherichia coli; Escherichia coli Proteins; Models, Molecular; Mutation; Protein Denaturation; Ribonuclease P; RNA, Catalytic; Spectrometry, Fluorescence; Tryptophan; Urea | 1997 |
Urea and thermal equilibrium denaturation studies on the dimerization domain of Escherichia coli Trp repressor.
Topics: Bacterial Proteins; Circular Dichroism; Dimerization; Escherichia coli; Molecular Weight; Peptide Fragments; Protein Denaturation; Protein Structure, Tertiary; Repressor Proteins; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Urea | 1997 |
Selective excitation of tryptophans in OmpF: a fluorescence emission study.
Topics: Bacterial Outer Membrane Proteins; Escherichia coli; Hydrogen-Ion Concentration; Iodides; Protein Folding; Spectrometry, Fluorescence; Tryptophan; Urea | 1997 |
Site-directed mutagenesis of either the highly conserved Trp-22 or the moderately conserved Trp-95 to a large, hydrophobic residue reduces the thermodynamic stability of a spectrin repeating unit.
Topics: Amino Acid Sequence; Animals; Chickens; Circular Dichroism; Dimerization; Humans; Molecular Sequence Data; Mutagenesis, Site-Directed; Peptides; Protein Denaturation; Protein Structure, Secondary; Recombinant Proteins; Scattering, Radiation; Solubility; Spectrin; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Structure-Activity Relationship; Thermodynamics; Tryptophan; Urea | 1997 |
Refolding of urea-denatured ovalbumin that comprises non-native disulfide isomers.
Topics: Calorimetry, Differential Scanning; Circular Dichroism; Disulfides; Isomerism; Models, Molecular; Ovalbumin; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Time Factors; Tryptophan; Urea | 1997 |
Effect of amino acid supplementation on whole-body protein turnover in Holstein steers.
Topics: Amino Acids; Animals; Arginine; Cattle; Diet; Dietary Supplements; Dose-Response Relationship, Drug; Histidine; Infusions, Parenteral; Lipids; Male; Methionine; Nitrogen Isotopes; Proteins; Threonine; Time Factors; Tryptophan; Urea; Zea mays | 1997 |
Time-resolved protein phosphorescence in the stopped-flow: denaturation of horse liver alcohol dehydrogenase by urea and guanidine hydrochloride.
Topics: Alcohol Dehydrogenase; Animals; Circular Dichroism; Guanidine; Horses; Kinetics; Liver; Luminescent Measurements; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Sodium Chloride; Spectrometry, Fluorescence; Spectrum Analysis; Tryptophan; Urea | 1997 |
Functional implications of disulfide bond, Cys45-Cys50, in recombinant prochymosin.
Topics: Acrylamide; Acrylamides; Animals; Caseins; Cattle; Chymosin; Circular Dichroism; Cysteine; Disulfides; Enzyme Activation; Enzyme Precursors; Enzyme Stability; Escherichia coli; Kinetics; Mutagenesis, Site-Directed; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Spectrometry, Fluorescence; Substrate Specificity; Tryptophan; Urea | 1997 |
Unfolding domains and tryptophan accessibility of a 59 kDa coiled-coil light meromyosin.
Topics: Circular Dichroism; Cross-Linking Reagents; Disulfides; Hot Temperature; Microscopy, Electron; Molecular Weight; Myosin Subfragments; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Solvents; Spectrometry, Fluorescence; Tryptophan; Urea | 1998 |
Conformation of polygalacturonase-II from Aspergillus carbonarius--a spectroscopic study.
Topics: Aspergillus; Bromosuccinimide; Circular Dichroism; Fungal Proteins; Molecular Weight; Polygalacturonase; Protein Conformation; Protein Denaturation; Protein Folding; Solutions; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Tryptophan; Urea | 1998 |
The aggregation in human lens proteins blocks the scavenging of UVA-generated singlet oxygen by ascorbic acid and glutathione.
Topics: Aging; Antioxidants; Ascorbic Acid; Crystallins; Cysteine; Fluorescence; Free Radical Scavengers; Glutathione; Histidine; Humans; Peroxides; Photolysis; Protein Conformation; Protein Denaturation; Reactive Oxygen Species; Sodium Azide; Tryptophan; Ultraviolet Rays; Urea | 1998 |
Spectroscopic analysis of halothane binding to the plasma membrane Ca2+-ATPase.
Topics: Acrylamide; Acrylamides; Anesthetics, Inhalation; Binding Sites; Calcium-Transporting ATPases; Ethers; Halothane; Kinetics; Phosphatidylcholines; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Tryptophan; Urea | 1998 |
Characterization of a partially folded monomer of the DNA-binding domain of human papillomavirus E2 protein obtained at high pressure.
Topics: Amino Acid Sequence; Dimerization; DNA-Binding Proteins; Fluorescence Polarization; Humans; Kinetics; Models, Molecular; Molecular Sequence Data; Oncogene Proteins, Viral; Papillomaviridae; Pressure; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins; Sequence Alignment; Sequence Homology, Amino Acid; Tryptophan; Urea | 1998 |
Anion-induced folding of Staphylococcal nuclease: characterization of multiple equilibrium partially folded intermediates.
Topics: Anions; Chlorides; Circular Dichroism; Hydrogen-Ion Concentration; Micrococcal Nuclease; Protein Conformation; Protein Denaturation; Protein Folding; Scattering, Radiation; Sulfates; Trichloroacetic Acid; Trifluoroacetic Acid; Tryptophan; Urea; X-Rays | 1998 |
Folding kinetics of a fluorescent variant of monomeric lambda repressor.
Topics: Amino Acid Substitution; Bacteriophage lambda; Circular Dichroism; DNA-Binding Proteins; Guanidine; Kinetics; Magnetic Resonance Spectroscopy; Models, Molecular; Mutagenesis, Site-Directed; Protein Denaturation; Protein Folding; Repressor Proteins; Spectrometry, Fluorescence; Tryptophan; Urea; Viral Proteins; Viral Regulatory and Accessory Proteins | 1998 |
Some aspects of beta-lactoglobulin structural properties in solution studied by fluorescence quenching.
Topics: Acrylamide; Binding Sites; Fluorescence; Kinetics; Lactoglobulins; Palmitic Acid; Protein Conformation; Protein Denaturation; Sodium Nitrite; Solutions; Spectrometry, Fluorescence; Tryptophan; Urea | 1998 |
Fluorescence quenching and time-resolved fluorescence studies on Momordica charantia (bitter gourd) seed lectin.
Topics: Acrylamide; Cesium; Fluorescence; Iodides; Lactose; Lectins; N-Glycosyl Hydrolases; Plant Proteins; Protein Denaturation; Ribosome Inactivating Proteins, Type 2; Spectrometry, Fluorescence; Tryptophan; Urea | 1998 |
Thermodynamic and kinetic characterization of calf lens gammaF-crystallin.
Topics: Animals; Cattle; Circular Dichroism; Crystallins; Guanidine; Kinetics; Lens, Crystalline; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Urea | 1998 |
Unfolding of Plasmodium falciparum triosephosphate isomerase in urea and guanidinium chloride: evidence for a novel disulfide exchange reaction in a covalently cross-linked mutant.
Topics: Animals; Chromatography, Gel; Circular Dichroism; Cross-Linking Reagents; Cysteine; Disulfides; Guanidine; Models, Molecular; Mutagenesis, Site-Directed; Plasmodium falciparum; Protein Conformation; Protein Denaturation; Recombinant Proteins; Spectrometry, Fluorescence; Triose-Phosphate Isomerase; Tryptophan; Tyrosine; Urea | 1999 |
Monitoring the sizes of denatured ensembles of staphylococcal nuclease proteins: implications regarding m values, intermediates, and thermodynamics.
Topics: Alanine; Amino Acid Substitution; Chromatography, Gel; Chromatography, High Pressure Liquid; Chymotrypsinogen; Micrococcal Nuclease; Mutagenesis, Site-Directed; Protein Denaturation; Reproducibility of Results; Spectrometry, Fluorescence; Thermodynamics; Threonine; Tryptophan; Urea; Valine | 1998 |
Role of carboxyl-terminal charges on S-modulin membrane affinity and inhibition of rhodopsin phosphorylation.
Topics: Amino Acid Sequence; Animals; Base Sequence; Calcium-Binding Proteins; Cattle; Cell Membrane; Cloning, Molecular; DNA Primers; Escherichia coli; Eye Proteins; Hippocalcin; Humans; Lipoproteins; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Nerve Tissue Proteins; Phylogeny; Polymerase Chain Reaction; Protein Structure, Secondary; Rana catesbeiana; Recombinant Fusion Proteins; Recombinant Proteins; Recoverin; Rhodopsin; Rod Cell Outer Segment; Sequence Alignment; Spectrometry, Fluorescence; Tryptophan; Urea | 1999 |
Foldability of barnase mutants obtained by permutation of modules or secondary structure units.
Topics: Amino Acid Sequence; Bacterial Proteins; Chromatography, Gel; Circular Dichroism; DNA Ligases; Escherichia coli; Molecular Sequence Data; Mutation; Nuclear Magnetic Resonance, Biomolecular; Peptide Fragments; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Fusion Proteins; Ribonucleases; Spectrometry, Fluorescence; Tryptophan; Ultracentrifugation; Ultraviolet Rays; Urea | 1999 |
Engineering out motion: introduction of a de novo disulfide bond and a salt bridge designed to close a dynamic cleft on the surface of cytochrome b5.
Topics: Animals; Cytochromes b5; Disulfides; Heme; Hot Temperature; Models, Molecular; Mutagenesis, Site-Directed; Oxidation-Reduction; Protein Binding; Protein Denaturation; Protein Engineering; Rats; Salts; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Urea | 1999 |
The structure and dynamics of partially folded actin.
Topics: Acrylamide; Actins; Models, Molecular; Protein Conformation; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Temperature; Tryptophan; Urea | 1999 |
Colicin E1 forms a dimer after urea-induced unfolding.
Topics: Amino Acid Sequence; Amino Acid Substitution; Chromatography, High Pressure Liquid; Circular Dichroism; Colicins; Dimerization; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Guanidine; Models, Molecular; Molecular Sequence Data; Naphthalenesulfonates; Protein Binding; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Spectrometry, Fluorescence; Tryptophan; Urea | 1999 |
Extremely rapid folding of the C-terminal domain of the prion protein without kinetic intermediates.
Topics: Amino Acid Substitution; Animals; Circular Dichroism; Disulfides; Half-Life; Hydrogen-Ion Concentration; Kinetics; Mice; Models, Molecular; Peptide Fragments; Prions; Protein Conformation; Protein Folding; Recombinant Proteins; Spectrometry, Fluorescence; Temperature; Thermodynamics; Tryptophan; Urea | 1999 |
Structure and stability of recombinant protein depend on the extra N-terminal methionine residue: S6 permutein from direct and fusion expression systems.
Topics: Calorimetry, Differential Scanning; Circular Dichroism; Escherichia coli; Genetic Vectors; Magnetic Resonance Spectroscopy; Methionine; Protein Conformation; Protein Folding; Recombinant Proteins; Ribosomal Protein S6; Ribosomal Proteins; Thermus thermophilus; Tryptophan; Urea | 1999 |
Conformational changes of urea-denatured colicin E1 induced by phospholipid membranes.
Topics: Chemical Phenomena; Chemistry, Physical; Circular Dichroism; Colicins; Dimyristoylphosphatidylcholine; Liposomes; Membrane Lipids; Phosphatidylethanolamines; Phosphatidylglycerols; Phospholipids; Protein Conformation; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Temperature; Tryptophan; Urea | 1999 |
Fluorescence and FTIR study of the pressure-induced denaturation of bovine pancreas trypsin.
Topics: Anilino Naphthalenesulfonates; Animals; Cattle; Pancreas; Pressure; Protein Denaturation; Protein Structure, Secondary; Spectrometry, Fluorescence; Spectroscopy, Fourier Transform Infrared; Thermodynamics; Trypsin; Tryptophan; Urea | 1999 |
The tert-butyl hydroperoxide-induced oxidation of actin Cys-374 is coupled with structural changes in distant regions of the protein.
Topics: Actins; Animals; Chymotrypsin; Cross-Linking Reagents; Cyanogen Bromide; Cysteine; Hydrolysis; Kinetics; Lysine; Oxidants; Peptide Fragments; Polymers; Protein Conformation; Protein Denaturation; Rabbits; Spectrometry, Fluorescence; Subtilisins; Sulfhydryl Compounds; tert-Butylhydroperoxide; Tryptophan; Urea | 1999 |
A stability transition at mildly acidic pH in the alpha-hemolysin (alpha-toxin) from Staphylococcus aureus.
Topics: Chromatography, Gel; Circular Dichroism; Fluorescence; Hydrogen-Ion Concentration; Protein Denaturation; Staphylococcus aureus; Thermodynamics; Tryptophan; Type C Phospholipases; Urea | 1999 |
Functional and conformational characterization of new mutants of heart fatty acid-binding protein.
Topics: Carrier Proteins; Cloning, Molecular; Fatty Acid-Binding Protein 7; Fatty Acid-Binding Proteins; Fatty Acids; Humans; Mutagenesis, Site-Directed; Myelin P2 Protein; Myocardium; Neoplasm Proteins; Nuclear Magnetic Resonance, Biomolecular; Oleic Acid; Phenylalanine; Protein Denaturation; Protein Structure, Quaternary; Recombinant Proteins; Sequence Analysis, DNA; Spectrometry, Fluorescence; Tryptophan; Tumor Suppressor Proteins; Urea | 1999 |
Fluorescence and circular dichroism spectroscopic studies on bovine lactoperoxidase.
Topics: Acrylamide; Animals; Cattle; Cesium; Circular Dichroism; Fluorescent Dyes; Guanidine; Iodine; Lactoperoxidase; Milk; Spectrometry, Fluorescence; Tryptophan; Urea | 1999 |
A new folding intermediate of apomyoglobin from Aplysia limacina: stepwise formation of a molten globule.
Topics: Allosteric Site; Amino Acid Sequence; Animals; Aplysia; Apoproteins; Circular Dichroism; Hot Temperature; Isoelectric Point; Kinetics; Models, Molecular; Molecular Sequence Data; Myoglobin; Potassium Chloride; Protein Denaturation; Protein Folding; Protein Renaturation; Protein Structure, Secondary; Solvents; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Urea; Whales | 2000 |
Probing the multidomain structure of the type I regulatory subunit of cAMP-dependent protein kinase using mutational analysis: role and environment of endogenous tryptophans.
Topics: Circular Dichroism; Cyclic AMP; Cyclic AMP-Dependent Protein Kinase RIalpha Subunit; Cyclic AMP-Dependent Protein Kinases; DNA Mutational Analysis; Enzyme Stability; Holoenzymes; Hot Temperature; Mutagenesis, Site-Directed; Potassium Iodide; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Fluorescence; Tryptophan; Urea | 2000 |
Aqueous two-phase systems containing urea: influence of protein structure on protein partitioning.
Topics: Bacteriophage T4; Escherichia coli; Muramidase; Protein Folding; Recombinant Proteins; Tryptophan; Urea | 2000 |
Domain-domain interface packing at conserved Trp-20 in class alpha glutathione transferase impacts on protein stability.
Topics: Amino Acid Sequence; Chromatography, Gel; Chromatography, High Pressure Liquid; Circular Dichroism; Enzyme Stability; Glutathione Transferase; Humans; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Folding; Tryptophan; Urea | 2000 |
Ca(2+)- and H(+)-dependent conformational changes of calbindin D(28k).
Topics: Anilino Naphthalenesulfonates; Animals; Calbindins; Calcium; Cattle; Chickens; Circular Dichroism; EF Hand Motifs; Escherichia coli; Humans; Hydrogen-Ion Concentration; Protein Binding; Protein Conformation; Protein Folding; Recombinant Proteins; S100 Calcium Binding Protein G; Spectrometry, Fluorescence; Spectrophotometry; Tryptophan; Urea | 2000 |
Fluorescence energy transfer indicates similar transient and equilibrium intermediates in staphylococcal nuclease folding.
Topics: Amino Acid Substitution; Anilino Naphthalenesulfonates; Anions; Circular Dichroism; Cysteine; Energy Transfer; Fluorescence; Guanidine; Hydrogen-Ion Concentration; Kinetics; Micrococcal Nuclease; Models, Molecular; Pliability; Proline; Protein Binding; Protein Denaturation; Protein Folding; Protein Renaturation; Protein Structure, Secondary; Thermodynamics; Titrimetry; Tryptophan; Urea | 2000 |
The annexin A3-membrane interaction is modulated by an N-terminal tryptophan.
Topics: Alanine; Annexin A3; Calcium; Electrophysiology; Humans; Liposomes; Membranes; Models, Molecular; Permeability; Phospholipids; Protein Conformation; Protein Denaturation; Protein Folding; Tryptophan; Urea | 2000 |
Systematic circular permutation of an entire protein reveals essential folding elements.
Topics: Binding Sites; Circular Dichroism; Escherichia coli; Kinetics; Models, Molecular; Molecular Sequence Data; Mutation; NADP; Peptide Fragments; Protein Binding; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Fluorescence; Tetrahydrofolate Dehydrogenase; Thermodynamics; Titrimetry; Tryptophan; Urea | 2000 |
Acid phosphatase reaction with peroxyl radicals: inactivation mechanism and behavior of the partially modified ensemble.
Topics: Acid Phosphatase; Amidines; Catalysis; Enzyme Stability; Fluorescence; Free Radicals; Hydrogen-Ion Concentration; Kinetics; Oxidants; Peroxides; Protein Denaturation; Solanum tuberosum; Temperature; Tryptophan; Urea | 2000 |
The slow folding reaction of barstar: the core tryptophan region attains tight packing before substantial secondary and tertiary structure formation and final compaction of the polypeptide chain.
Topics: Bacterial Proteins; Circular Dichroism; Fluorescence Polarization; Kinetics; Peptides; Protein Denaturation; Protein Folding; Protein Renaturation; Protein Structure, Secondary; Protein Structure, Tertiary; Rotation; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Urea; Water | 2000 |
Use of domain specific ligands to study urea-induced unfolding of bovine serum albumin.
Topics: Animals; Bilirubin; Cattle; Chloroform; Diazepam; Dose-Response Relationship, Drug; Fluorescence; Ligands; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Serum Albumin; Spectrometry, Fluorescence; Tryptophan; Urea | 2000 |
The interaction of the B-ring of colchicine with alpha-tubulin: a novel footprinting approach.
Topics: Amino Acid Sequence; Animals; Bibenzyls; Binding Sites; Cattle; Chromatography, High Pressure Liquid; Colchicine; Fluorescence; Models, Molecular; Molecular Sequence Data; Peptide Fragments; Podophyllotoxin; Protein Conformation; Protein Footprinting; Stilbenes; Tropolone; Tryptophan; Tubulin; Urea | 2000 |
Differential stabilization of two hydrophobic cores in the transition state of the villin 14T folding reaction.
Topics: Amino Acid Sequence; Amino Acid Substitution; Animals; Binding Sites; Carrier Proteins; Chickens; Conserved Sequence; Evolution, Molecular; Fluorescence; Kinetics; Microfilament Proteins; Models, Molecular; Molecular Sequence Data; Peptide Fragments; Point Mutation; Protein Denaturation; Protein Folding; Protein Renaturation; Protein Structure, Secondary; Protein Structure, Tertiary; Thermodynamics; Tryptophan; Urea; Water | 2000 |
The metastable state of nucleocapsids of enveloped viruses as probed by high hydrostatic pressure.
Topics: Alphavirus; Anilino Naphthalenesulfonates; Animals; Cell Line; Cell Membrane; Chromatography, Gel; Chromatography, High Pressure Liquid; Cricetinae; Ethidium; Fluorescent Dyes; Hydrostatic Pressure; Light; Models, Biological; Nucleocapsid Proteins; Pressure; Protein Binding; Scattering, Radiation; Spectrometry, Fluorescence; Tryptophan; Urea; Viruses | 2001 |
Structural stabilization of [2Fe-2S] ferredoxin from Halobacterium salinarum.
Topics: Circular Dichroism; Ferredoxins; Fluorescence Polarization; Halobacterium salinarum; Kinetics; Protein Conformation; Protein Folding; Protein Isoforms; Protein Structure, Secondary; Protein Structure, Tertiary; Salts; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Urea | 2001 |
Urea equilibrium unfolding of the major core protein of the retrovirus feline immunodeficiency virus and its tryptophan mutants.
Topics: Circular Dichroism; Cloning, Molecular; Gene Products, gag; Immunodeficiency Virus, Feline; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Urea; Viral Core Proteins | 2001 |
Hierarchical folding of intestinal fatty acid binding protein.
Topics: Amino Acid Substitution; Animals; Carrier Proteins; Fatty Acid-Binding Protein 7; Fatty Acid-Binding Proteins; Kinetics; Mutagenesis, Site-Directed; Neoplasm Proteins; Nerve Tissue Proteins; Phenylalanine; Protein Denaturation; Protein Folding; Rats; Recombinant Proteins; Spectrometry, Fluorescence; Tryptophan; Urea | 2001 |
Free energies of urea and of thermal unfolding show that two tandem repeats of spectrin are thermodynamically more stable than a single repeat.
Topics: Amino Acid Motifs; Animals; Chickens; Circular Dichroism; Models, Chemical; Mutagenesis, Site-Directed; Peptide Fragments; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Repetitive Sequences, Amino Acid; Spectrin; Spectrometry, Fluorescence; Temperature; Thermodynamics; Tryptophan; Urea | 2001 |
Monovalent cation-induced conformational change in glucose oxidase leading to stabilization of the enzyme.
Topics: Aspergillus niger; Binding Sites; Bromosuccinimide; Calorimetry, Differential Scanning; Cations, Monovalent; Chromatography, Gel; Circular Dichroism; Cross-Linking Reagents; Endopeptidases; Enzyme Activation; Enzyme Stability; Glucose; Glucose Oxidase; Glutaral; Hot Temperature; Hydrogen-Ion Concentration; Hydrolysis; Kinetics; Lithium Chloride; Potassium Chloride; Protein Conformation; Protein Denaturation; Sodium Chloride; Spectrometry, Fluorescence; Structure-Activity Relationship; Tryptophan; Urea | 2001 |
Association and activation of fructose 1,6-bisphosphase during unfolding and refolding: spectroscopic and enzymatic studies.
Topics: Amino Acid Substitution; Circular Dichroism; Enzyme Activation; Fluorescence; Fructose-Bisphosphatase; Guanidine; Magnesium; Mutation; Protein Folding; Spectrometry, Fluorescence; Tryptophan; Urea | 2001 |
Snapshots of protein folding. A study on the multiple transition state pathway of cytochrome c(551) from Pseudomonas aeruginosa.
Topics: Acids; Bacterial Proteins; Cytochrome c Group; Fluorescence; Glutamic Acid; Guanidine; Hydrogen Bonding; Kinetics; Models, Molecular; Mutation; Protein Conformation; Protein Denaturation; Protein Folding; Pseudomonas aeruginosa; Static Electricity; Thermodynamics; Tryptophan; Urea | 2001 |
Structure and dynamics of the alpha-lactalbumin molten globule: fluorescence studies using proteins containing a single tryptophan residue.
Topics: Amino Acid Substitution; Circular Dichroism; Fluorescence Polarization; Humans; Lactalbumin; Mutagenesis, Site-Directed; Nuclear Magnetic Resonance, Biomolecular; Phenylalanine; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins; Solvents; Spectrometry, Fluorescence; Structure-Activity Relationship; Thermodynamics; Tryptophan; Urea | 2001 |
Substitutions of Thr-103-Ile and Trp-138-Gly in amidase from Pseudomonas aeruginosa are responsible for altered kinetic properties and enzyme instability.
Topics: Amidohydrolases; Enzyme-Linked Immunosorbent Assay; Glycine; Hydrogen-Ion Concentration; Isoleucine; Kinetics; Mutagenesis, Site-Directed; Mutation; Plasmids; Polymerase Chain Reaction; Pseudomonas aeruginosa; Temperature; Threonine; Tryptophan; Urea | 2001 |
Detection of disulfide bonds in bovine brain tubulin and their role in protein folding and microtubule assembly in vitro: a novel disulfide detection approach.
Topics: Ammonium Sulfate; Animals; Brain Chemistry; Cattle; Colchicine; Cysteine; Disulfides; Dithiothreitol; Microtubules; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Reducing Agents; Spectrometry, Fluorescence; Tryptophan; Tubulin; Urea | 2001 |
Effects of five-tryptophan mutations on structure, stability and function of Escherichia coli dihydrofolate reductase.
Topics: Circular Dichroism; Enzyme Stability; Escherichia coli; Mutagenesis, Site-Directed; Protein Folding; Spectrometry, Fluorescence; Temperature; Tetrahydrofolate Dehydrogenase; Thermodynamics; Tryptophan; Urea | 2001 |
An engineered transthyretin monomer that is nonamyloidogenic, unless it is partially denatured.
Topics: Amino Acid Substitution; Amyloid; Binding Sites; Crystallography, X-Ray; Dimerization; Drug Stability; Fluorescent Dyes; Guanidine; Humans; Hydrogen-Ion Concentration; Macromolecular Substances; Models, Molecular; Mutagenesis, Site-Directed; Nuclear Magnetic Resonance, Biomolecular; Prealbumin; Protein Denaturation; Protein Structure, Secondary; Recombinant Proteins; Spectrometry, Fluorescence; Tryptophan; Ultracentrifugation; Urea | 2001 |
Mass spectrometric analysis of a UV-cross-linked protein-DNA complex: tryptophans 54 and 88 of E. coli SSB cross-link to DNA.
Topics: Amino Acids; Bacterial Proteins; Cross-Linking Reagents; DNA; DNA-Binding Proteins; Endopeptidase K; Escherichia coli; Peptides; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tryptophan; Ultraviolet Rays; Urea | 2001 |
p53 unfolding detected by CD but not by tryptophan fluorescence.
Topics: Circular Dichroism; Guanidine; Humans; Potassium Iodide; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Tryptophan; Tumor Suppressor Protein p53; Urea | 2001 |
Probing the equilibrium denaturation of the serpin alpha(1)-antitrypsin with single tryptophan mutants; evidence for structure in the urea unfolded state.
Topics: alpha 1-Antitrypsin; Circular Dichroism; Guanidines; Humans; Models, Molecular; Mutation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Spectrometry, Fluorescence; Structure-Activity Relationship; Thermodynamics; Thiocyanates; Thrombin; Trypsin Inhibitors; Tryptophan; Urea | 2001 |
Effect of denaturants on the structural properties of soybean lipoxygenase-1.
Topics: Circular Dichroism; Enzyme Stability; Glycine max; Hot Temperature; Lipoxygenase; Pressure; Protein Denaturation; Protein Structure, Tertiary; Spectrometry, Fluorescence; Tryptophan; Urea | 2001 |
Real-time and equilibrium (19)F-NMR studies reveal the role of domain-domain interactions in the folding of the chaperone PapD.
Topics: Bacterial Proteins; Biophysical Phenomena; Biophysics; Circular Dichroism; Escherichia coli; Escherichia coli Proteins; Fluorine; Humans; Magnetic Resonance Spectroscopy; Molecular Chaperones; Mutagenesis, Site-Directed; Periplasmic Proteins; Protein Folding; Protein Structure, Tertiary; Tryptophan; Urea | 2002 |
Cross-adaptation and bitterness inhibition of L-tryptophan, L-phenylalanine and urea: further support for shared peripheral physiology.
Topics: Adaptation, Physiological; Adult; Dose-Response Relationship, Drug; Female; Humans; Male; Middle Aged; Phenylalanine; Taste; Taste Threshold; Tryptophan; Urea | 2002 |
Tryptophan metabolism via the kynurenine pathway in experimental chronic renal failure.
Topics: Animals; Chromatography, High Pressure Liquid; Creatinine; Kidney; Kidney Failure, Chronic; Kynurenic Acid; Kynurenine; Male; ortho-Aminobenzoates; Quinolinic Acid; Rats; Rats, Wistar; Time Factors; Tryptophan; Urea; Xanthurenates | 2002 |
Probing the binding states of GDP to Cdc42 using urea interaction.
Topics: cdc42 GTP-Binding Protein; Electron Spin Resonance Spectroscopy; Guanosine Diphosphate; Humans; Magnesium; Manganese; Models, Molecular; Spectrometry, Fluorescence; Tryptophan; Urea; Water | 2002 |
Effect of urea denaturation on tryptophan fluorescence and nucleotide binding on tubulin studied by fluorescence and NMR spectroscopic methods.
Topics: Acrylamide; Animals; Brain; Dose-Response Relationship, Drug; Goats; Guanosine Triphosphate; Hydrogen-Ion Concentration; Magnetic Resonance Spectroscopy; Nucleotides; Protein Binding; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Time Factors; Tryptophan; Tubulin; Urea | 2001 |
Comparison of two methods for radioiodination on the oxidizability properties of low density lipoprotein.
Topics: Adult; alpha-Tocopherol; Antioxidants; Chlorides; Copper; Humans; Indicators and Reagents; Iodides; Iodine Radioisotopes; Isotope Labeling; Lipoproteins, LDL; Oxidation-Reduction; Thiobarbituric Acid Reactive Substances; Tryptophan; Urea | 2001 |
Half-of-the-sites reactivity of F235C lambda-repressor: implications for the structure of the whole repressor.
Topics: Acrylamide; Circular Dichroism; Cysteine; DNA-Binding Proteins; Fluorescent Dyes; Mutagenesis, Site-Directed; Protein Denaturation; Protein Structure, Tertiary; Repressor Proteins; Spectrometry, Fluorescence; Sulfhydryl Compounds; Tryptophan; Urea; Viral Proteins; Viral Regulatory and Accessory Proteins | 2002 |
Differential conformational environment of tryptophan in epsilon native prototoxin and active toxin from Clostridium perfringens type D.
Topics: Animals; Bacterial Toxins; Bromosuccinimide; Circular Dichroism; Clostridium perfringens; Protein Conformation; Protein Precursors; Spectrometry, Fluorescence; Tryptophan; Urea | 2002 |
Exploring the role of amino acid-18 of the leucine binding proteins of E. coli.
Topics: Amino Acid Sequence; Amino Acids, Branched-Chain; Bacterial Proteins; Crystallography, X-Ray; Escherichia coli; Genes, Bacterial; Leucine; Ligands; Membrane Proteins; Models, Molecular; Mutagenesis, Site-Directed; Nuclear Magnetic Resonance, Biomolecular; Point Mutation; Protein Conformation; Protein Denaturation; Protein Structure, Tertiary; Structure-Activity Relationship; Substrate Specificity; Tryptophan; Urea | 2002 |
Differential salt-induced stabilization of structure in the initial folding intermediate ensemble of barstar.
Topics: Bacterial Proteins; Enzyme Inhibitors; Kinetics; Magnesium Chloride; Plasmids; Potassium Chloride; Protein Conformation; Protein Denaturation; Protein Folding; Ribonucleases; Spectrophotometry; Tryptophan; Urea | 2002 |
Folding and assembly of lambda Cro repressor dimers are kinetically limited by proline isomerization.
Topics: Bacteriophage lambda; Carrier Proteins; Catalysis; Circular Dichroism; Dimerization; DNA-Binding Proteins; Escherichia coli Proteins; Fluorescence Resonance Energy Transfer; Hydrogen-Ion Concentration; Kinetics; Peptidylprolyl Isomerase; Proline; Protein Denaturation; Protein Folding; Protein Processing, Post-Translational; Repressor Proteins; Tryptophan; Urea; Viral Proteins; Viral Regulatory and Accessory Proteins | 2002 |
Surface expansion is independent of and occurs faster than core solvation during the unfolding of barstar.
Topics: Bacterial Proteins; Cysteine; Fluorescence Resonance Energy Transfer; Kinetics; Mutagenesis, Site-Directed; Protein Denaturation; Protein Folding; Ribonucleases; Solvents; Surface Properties; Trinitrobenzenesulfonic Acid; Tryptophan; Urea | 2003 |
Unusual fluorescence of W168 in Plasmodium falciparum triosephosphate isomerase, probed by single-tryptophan mutants.
Topics: Animals; Circular Dichroism; Fluorescence; Glycolates; Guanidine; Mass Spectrometry; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Plasmodium falciparum; Protein Binding; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Triose-Phosphate Isomerase; Tryptophan; Urea | 2003 |
Observed hysteresis of virus capsid disassembly is implicit in kinetic models of assembly.
Topics: Capsid; Dimerization; Dose-Response Relationship, Drug; Escherichia coli; Guanidine; Hepatitis B Core Antigens; Hepatitis B virus; Kinetics; Light; Scattering, Radiation; Thermodynamics; Time Factors; Tryptophan; Urea; Viral Core Proteins; Virion; Virus Assembly | 2003 |
Involvement of single residue tryptophan 548 in the quaternary structural stability of pigeon cytosolic malic enzyme.
Topics: Amino Acid Substitution; Animals; Columbidae; Cytosol; Dimerization; Enzyme Stability; Malate Dehydrogenase; Manganese; Protein Conformation; Protein Denaturation; Protein Structure, Quaternary; Tryptophan; Urea | 2003 |
Conformational coupling of Mg2+ and Ca2+ on the three-state folding of calexcitin B.
Topics: Binding Sites; Caenorhabditis elegans Proteins; Calcium; Calcium-Binding Proteins; Circular Dichroism; Cysteine; GTP-Binding Proteins; Hot Temperature; Kinetics; Magnesium; Potassium Channels; Protein Conformation; Protein Denaturation; Protein Folding; Sarcoplasmic Reticulum; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds; Thermodynamics; Tryptophan; Urea | 2003 |
Regulation of RecA protein binding to DNA by opposing effects of ATP and ADP on inter-domain contacts: analysis by urea-induced unfolding of wild-type and C-terminal truncated RecA.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Circular Dichroism; DNA; DNA, Bacterial; DNA, Single-Stranded; Escherichia coli; Ligands; Models, Molecular; Nucleic Acid Conformation; Protein Binding; Protein Conformation; Protein Folding; Protein Structure, Tertiary; Rec A Recombinases; Recombination, Genetic; Spectrometry, Fluorescence; Tryptophan; Urea | 2003 |
Activation mechanism of HSP16.5 from Methanococcus jannaschii.
Topics: Anilino Naphthalenesulfonates; Archaeal Proteins; Fluorescent Dyes; Heat-Shock Proteins; Hot Temperature; Methanococcus; Plant Proteins; Protein Conformation; Spectrometry, Fluorescence; Tryptophan; Urea | 2003 |
Unfolding of Vibrio cholerae hemolysin induces oligomerization of the toxin monomer.
Topics: Cell Membrane; Cholesterol; Diffusion; Electrophoresis, Polyacrylamide Gel; Guanidine; Hemolysin Proteins; Lipid Metabolism; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Sodium Dodecyl Sulfate; Spectrometry, Fluorescence; Sphingolipids; Thermodynamics; Toxins, Biological; Tryptophan; Urea; Vibrio cholerae | 2003 |
Crystal structures of engrailed homeodomain mutants: implications for stability and dynamics.
Topics: Animals; Crystallography, X-Ray; Drosophila; Homeodomain Proteins; Humans; Hydrogen; Indoles; Leucine; Magnetic Resonance Spectroscopy; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Protein Conformation; Protein Structure, Tertiary; Software; Transcription Factors; Tryptophan; Urea; Water | 2003 |
The stability of human acidic beta-crystallin oligomers and hetero-oligomers.
Topics: Animals; beta-Crystallin A Chain; beta-Crystallin B Chain; beta-Crystallins; Cattle; Chemical Precipitation; Chromatography, Ion Exchange; Circular Dichroism; Hot Temperature; Humans; Protein Denaturation; Rats; Recombinant Proteins; Spectrometry, Fluorescence; Tryptophan; Urea | 2003 |
Rapid unfolding of a domain populates an aggregation-prone intermediate that can be recognized by GroEL.
Topics: Amino Acid Substitution; Bacteriophage P22; Capsid Proteins; Chaperonin 60; Humans; Protein Denaturation; Protein Folding; Protein Structure, Quaternary; Protein Structure, Tertiary; Thermodynamics; Tryptophan; Urea | 2003 |
[Colorimetric determination of urea with tryptophan-diacetyl reagent].
Topics: Colorimetry; Diacetyl; Humans; Indicators and Reagents; Tryptophan; Urea | 1953 |
Effectiveness of urea and ammonium nitrogen for the synthesis of dispensable amino acids by the chick.
Topics: Amino Acids; Ammonium Compounds; Animals; Arginine; Chickens; Glycine; Histidine; Isoleucine; Leucine; Lysine; Methionine; Nitrogen; Phenylalanine; Quaternary Ammonium Compounds; Threonine; Tryptophan; Tyrosine; Urea; Valine | 1962 |
THE SOLUBILITY OF AMINO ACIDS AND RELATED COMPOUNDS IN AQUEOUS UREA SOLUTIONS.
Topics: Alanine; Amino Acids; Asparagine; Chemical Phenomena; Chemistry; Glutamine; Glycine; Histidine; Leucine; Methionine; Peptides; Phenylalanine; Research; Solubility; Solvents; Spectrophotometry; Threonine; Tryptophan; Tyrosine; Urea | 1963 |
THE ENZYME-SUBSTRATE COMPLEX IN A MURAMIDASE CATALYZED REACTION. I. DIFFERENCE SPECTRUM OF COMPLEX.
Topics: Chemical Phenomena; Chemistry; Glucosamine; Hydrogen-Ion Concentration; Muramidase; Ovalbumin; Polysaccharides; Pyrrolidinones; Research; Spectrum Analysis; Tryptophan; Tyrosine; Urea | 1963 |
THE DETECTION OF SOME INDOLES AND RELATED COMPOUNDS ON PAPER CHROMATOGRAMS.
Topics: 4-Aminobenzoic Acid; Amino Acids; Chromatography; Citrulline; Indicators and Reagents; Indoleacetic Acids; Indoles; Kynurenine; ortho-Aminobenzoates; Phenols; Quinolines; Research; Thiourea; Tryptophan; Urea; Xanthurenates | 1964 |
STRUCTURAL TRANSITIONS OF LYSOZYME.
Topics: Chemistry Techniques, Analytical; Fluorescence; Guanidine; Guanidines; Hydrogen-Ion Concentration; Muramidase; Research; Spectrum Analysis; Temperature; Tryptophan; Urea | 1964 |
[THE PHYSIOPATHOLOGY AND TREATMENT OF HEPATIC COMA].
Topics: Alkalosis; Ammonia; Anti-Bacterial Agents; Antibiotics, Antitubercular; Arginine; Bilirubin; Blood Coagulation Disorders; Blood Glucose; Cortisone; Gastrointestinal Hemorrhage; Hepatic Encephalopathy; Humans; Hypoglycemia; Liver; Metabolism; Tryptophan; Urea; Water-Electrolyte Balance | 1964 |
ACTION OF N-BROMOSUCCINIMIDE ON SPERM WHALE MYOGLOBIN.
Topics: Animals; Bromosuccinimide; Cetacea; Chemical Phenomena; Chemistry; Hemoglobins; Hydrogen-Ion Concentration; Myoglobin; Pyrrolidinones; Research; Spectrophotometry; Sperm Whale; Sucrose; Tryptophan; Urea | 1964 |
[THE DETERMINATION OF SOME BIOCHEMICAL CONSTANTS IN THE SERUM IN SPORTSMEN DURING INTENSIVE TRAINING].
Topics: Blood Chemical Analysis; Blood Glucose; Blood Protein Electrophoresis; Blood Urea Nitrogen; Glycoproteins; Hexoses; Humans; Liver Function Tests; Neuraminic Acids; Physical Exertion; Sports Medicine; Tryptophan; Urea | 1964 |
ULTRAVIOLET FLUORESCENCE OF PROTEINS: INFLUENCE OF CONFORMATION AND ENVIRONMENT.
Topics: Animals; Cattle; Chemical Phenomena; Chemistry, Physical; Enzyme Precursors; Fluorescence; Muramidase; Pepsin A; Proteins; Serum Albumin; Serum Albumin, Bovine; Solvents; Spectrum Analysis; Thyroglobulin; Trypsin Inhibitors; Tryptophan; Urea | 1964 |
CONFORMATIONAL STUDIES OF WHEAT GLUTEN, GLUTENIN, AND GLIADIN IN UREA SOLUTIONS AT VARIOUS PH'S.
Topics: Chemical Phenomena; Chemistry, Physical; Gliadin; Glutens; Hydrogen-Ion Concentration; Plant Proteins; Research; Solutions; Spectrophotometry; Triticum; Tryptophan; Tyrosine; Ultracentrifugation; Urea; Viscosity | 1964 |
NATIVE AND UNFOLDED STATES OF PEPSINOGEN. I. THE MOLECULAR CONFORMATION IN WATER AND IN UREA.
Topics: Enzyme Precursors; Fluorescence; Hydrogen-Ion Concentration; Indicators and Reagents; Kinetics; Molecular Conformation; Pepsinogen A; Pepsinogens; Research; Spectrophotometry; Tryptophan; Urea; Viscosity; Water | 1965 |
AMINO ACID ISOMERIZATION IN THE PRODUCTION OF L-PHENYLALANINE FROM D-PHENYLALANINE BY BACTERIA.
Topics: Amino Acids; Ammonium Compounds; Centrifugation; Colorimetry; Culture Media; D-Amino-Acid Oxidase; Manometry; Methionine; Oxygen; Pharmacology; Phenylalanine; Phenylpyruvic Acids; Photometry; Pseudomonas; Quaternary Ammonium Compounds; Research; Threonine; Transaminases; Tryptophan; Urea; Valine | 1965 |
CARP MYOGENS OF WHITE AND RED MUSCLES. PROPERTIES AND AMINO ACID COMPOSITION OF THE MAIN LOW-MOLECULAR-WEIGHT COMPONENTS OF WHITE MUSCLE.
Topics: Amino Acids; Animals; Biochemical Phenomena; Biochemistry; Carps; Chemical Phenomena; Chemistry Techniques, Analytical; Chemistry, Physical; Crystallization; Cysteine; Electrophoresis; Fishes; Molecular Weight; Muscle Proteins; Muscles; Protein Denaturation; Research; Tryptophan; Ultracentrifugation; Ultraviolet Rays; Urea; Viscosity | 1965 |
RECENT DEVELOPMENTS IN THE UNDERSTANDING OF AMINO-ACID AND PROTEIN METABOLISM BY SCHISTOSOMA MANSONI IN VITRO.
Topics: Alanine; Amino Acids; Animals; Arginase; Arginine; Aspartic Acid; Carbon Isotopes; Glucose; Hemoglobins; Histidine; In Vitro Techniques; Ornithine; Peptides; Proline; Proteins; Radiometry; Research; Schistosoma; Schistosoma mansoni; Tryptophan; Urea | 1965 |
cAMP-dependent protein kinase regulatory subunit type IIbeta: active site mutations define an isoform-specific network for allosteric signaling by cAMP.
Topics: Allosteric Site; Amino Acid Sequence; Animals; Arginine; Binding Sites; Biotin; Circular Dichroism; Cyclic AMP; Cyclic AMP-Dependent Protein Kinase RIIbeta Subunit; Cyclic AMP-Dependent Protein Kinases; Dose-Response Relationship, Drug; Kinetics; Lysine; Maleimides; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Protein Binding; Protein Folding; Protein Isoforms; Protein Structure, Tertiary; Rats; Sequence Homology, Amino Acid; Signal Transduction; Spectrometry, Fluorescence; Structure-Activity Relationship; Surface Plasmon Resonance; Thermodynamics; Time Factors; Tryptophan; Ultraviolet Rays; Urea | 2004 |
Role of conformational flexibility for enzymatic activity in NADH oxidase from Thermus thermophilus.
Topics: Binding Sites; Catalysis; Circular Dichroism; Crystallography, X-Ray; Dimerization; Flavins; Kinetics; Models, Molecular; Multienzyme Complexes; NADH, NADPH Oxidoreductases; Protein Conformation; Spectrometry, Fluorescence; Temperature; Thermodynamics; Thermus thermophilus; Time Factors; Tryptophan; Urea | 2003 |
Substitutions at tyrosine 66 of Escherichia coli uracil DNA glycosylase lead to characterization of an efficient enzyme that is recalcitrant to product inhibition.
Topics: Amino Acid Substitution; Binding Sites; Catalysis; DNA; DNA Glycosylases; Escherichia coli; Fluorescence; Kinetics; Models, Molecular; Mutation; Protein Conformation; Tryptophan; Tyrosine; Uracil; Uracil-DNA Glycosidase; Urea; Viral Proteins | 2003 |
Mutations in the hydrophobic core and in the protein-RNA interface affect the packing and stability of icosahedral viruses.
Topics: Capsid Proteins; Chromatography, Gel; Circular Dichroism; Dimerization; Escherichia coli; Iridoviridae; Levivirus; Models, Molecular; Protein Conformation; Protein Denaturation; Protein Structure, Secondary; Proteins; RNA; Spectrometry, Fluorescence; Tryptophan; Urea | 2004 |
Biophysical analyses of the transthyretin variants, Tyr114His and Tyr116Ser, associated with familial amyloidotic polyneuropathy.
Topics: Amino Acid Substitution; Amyloid; Amyloid Neuropathies, Familial; Dimerization; Electrophoresis, Polyacrylamide Gel; Genetic Variation; Histidine; Humans; Hydrogen-Ion Concentration; Prealbumin; Protein Isoforms; Protein Structure, Quaternary; Serine; Spectrometry, Fluorescence; Tryptophan; Tyrosine; Urea | 2003 |
Folding of Aplysia limacina apomyoglobin involves an intermediate in common with other evolutionarily distant globins.
Topics: Amino Acid Substitution; Animals; Aplysia; Apoproteins; Evolution, Molecular; Globins; Heme; Mutagenesis, Site-Directed; Myoglobin; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Tyrosine; Urea; Whales | 2004 |
Urea-dependent unfolding of murine adenosine deaminase: sequential destabilization as measured by 19F NMR.
Topics: Adenosine Deaminase; Animals; Circular Dichroism; Enzyme Stability; Fluorine; Magnetic Resonance Spectroscopy; Mice; Mutagenesis, Site-Directed; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Fluorescence; Tryptophan; Urea | 2004 |
Role of hydration in the conformational transitions between unliganded and liganded forms of loop 13 of the Na+/glucose cotransporter 1.
Topics: Acrylamide; Anilino Naphthalenesulfonates; Circular Dichroism; Glycerol; Hydrophobic and Hydrophilic Interactions; Kinetics; Ligands; Membrane Glycoproteins; Monosaccharide Transport Proteins; Phlorhizin; Protein Binding; Protein Conformation; Protein Denaturation; Sodium-Glucose Transporter 1; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Urea; Water | 2004 |
Elimination of a misfolded folding intermediate by a single point mutation.
Topics: Allergens; Amino Acid Sequence; Amino Acid Substitution; Antigens, Plant; Hydrophobic and Hydrophilic Interactions; Kinetics; Models, Chemical; Molecular Sequence Data; Plant Proteins; Point Mutation; Protein Denaturation; Protein Folding; Signal Transduction; Spectrometry, Fluorescence; Structural Homology, Protein; Thermodynamics; Tryptophan; Tyrosine; Urea | 2004 |
Early events during folding of wild-type staphylococcal nuclease and a single-tryptophan variant studied by ultrarapid mixing.
Topics: Anilino Naphthalenesulfonates; Circular Dichroism; Fluorescent Dyes; Micrococcal Nuclease; Models, Chemical; Models, Molecular; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Tryptophan; Urea | 2004 |
Reshaping the folding energy landscape by chloride salt: impact on molten-globule formation and aggregation behavior of carbonic anhydrase.
Topics: Anilino Naphthalenesulfonates; Carbonic Anhydrase II; Cross-Linking Reagents; Escherichia coli Proteins; Humans; Lithium Chloride; Protein Conformation; Protein Denaturation; Protein Folding; Sodium Chloride; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Urea | 2004 |
Functional dissection and molecular characterization of calcium-sensitive actin-capping and actin-depolymerizing sites in villin.
Topics: Actins; Alanine; Aspartic Acid; Binding Sites; Calcium; Cloning, Molecular; DNA Mutational Analysis; Dose-Response Relationship, Drug; Escherichia coli; Gelsolin; Humans; Kinetics; Magnetic Resonance Spectroscopy; Microfilament Proteins; Models, Chemical; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Thermodynamics; Time Factors; Tryptophan; Urea | 2004 |
Human glycolipid transfer protein: probing conformation using fluorescence spectroscopy.
Topics: Amino Acid Sequence; Animals; Carrier Proteins; Cattle; Cloning, Molecular; Glycolipids; Humans; Liposomes; Mice; Molecular Sequence Data; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Spectrometry, Fluorescence; Swine; Tryptophan; Urea | 2004 |
Redox regulation of OxyR requires specific disulfide bond formation involving a rapid kinetic reaction path.
Topics: Disulfides; DNA-Binding Proteins; Escherichia coli; Escherichia coli Proteins; Kinetics; Models, Molecular; Oxidants; Oxidation-Reduction; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Repressor Proteins; Spectrometry, Fluorescence; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Thermodynamics; Transcription Factors; Tryptophan; Urea | 2004 |
Redox sensitive cysteine residues in calbindin D28k are structurally and functionally important.
Topics: Amino Acid Sequence; Amino Acid Substitution; Anilino Naphthalenesulfonates; Buffers; Calbindin 1; Calbindins; Calcium; Circular Dichroism; Cysteine; Enzyme Activation; Glutathione; Humans; Molecular Sequence Data; Mutagenesis, Site-Directed; Oxidation-Reduction; Phosphoric Monoester Hydrolases; Protein Binding; Protein Denaturation; Protein Folding; S100 Calcium Binding Protein G; Spectrometry, Fluorescence; Structure-Activity Relationship; Tryptophan; Urea | 2005 |
Dissecting contributions to the denaturant sensitivities of proteins.
Topics: Amino Acid Sequence; Circular Dichroism; Guanidine; Molecular Sequence Data; Peptides; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Solutions; Thermodynamics; Tryptophan; Urea | 2005 |
Attachment of a histidine tag to the minimal zinc finger protein of the Aspergillus nidulans gene regulatory protein AreA causes a conformational change at the DNA-binding site.
Topics: Amino Acid Sequence; Aspergillus nidulans; Binding Sites; Cysteine; Fungal Proteins; Gene Expression Regulation, Fungal; Genetic Vectors; Histidine; Kinetics; Molecular Sequence Data; Protein Binding; Protein Conformation; Protein Denaturation; Protein Structure, Tertiary; Spectrometry, Fluorescence; Thrombin; Tryptophan; Urea; Zinc Fingers | 2005 |
Effects of heme on the structure of the denatured state and folding kinetics of cytochrome b562.
Topics: Amino Acid Sequence; Apoproteins; Circular Dichroism; Cytochrome b Group; Escherichia coli; Escherichia coli Proteins; Guanidine; Heme; Hydrogen-Ion Concentration; Kinetics; Models, Molecular; Molecular Sequence Data; Mutation; Nuclear Magnetic Resonance, Biomolecular; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Thermodynamics; Tryptophan; Urea | 2005 |
Conformational study of spectrin in presence of submolar concentrations of denaturants.
Topics: 2-Naphthylamine; Animals; Binding Sites; Dimerization; Fluorescence Polarization; Fluorescent Dyes; Guanidine; In Vitro Techniques; Protein Conformation; Protein Denaturation; Protein Structure, Quaternary; Sheep; Spectrin; Tryptophan; Urea | 2005 |
Dissecting the structural determinants of the stability of cholesterol oxidase containing covalently bound flavin.
Topics: Anilino Naphthalenesulfonates; Brevibacterium; Calorimetry; Carbon Monoxide; Catalysis; Cholesterol Oxidase; Circular Dichroism; DNA, Complementary; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Fatty Acids, Unsaturated; Flavins; Hot Temperature; Hydrogen Peroxide; Hydrogen-Ion Concentration; Models, Chemical; Mutation; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Spectrometry, Fluorescence; Spectrophotometry; Temperature; Thermodynamics; Tryptophan; Ultraviolet Rays; Urea | 2005 |
The kinetics of PDZ domain-ligand interactions and implications for the binding mechanism.
Topics: Adaptor Proteins, Signal Transducing; Disks Large Homolog 4 Protein; DNA, Complementary; Dose-Response Relationship, Drug; Humans; Hydrogen-Ion Concentration; Intracellular Signaling Peptides and Proteins; Ions; Kinetics; Ligands; Membrane Proteins; Models, Molecular; Mutation; Peptides; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Protein Tyrosine Phosphatase, Non-Receptor Type 13; Protein Tyrosine Phosphatases; Spectrophotometry; Static Electricity; Temperature; Thermodynamics; Time Factors; Tryptophan; Urea | 2005 |
The role of Trp-82 in the folding of intestinal fatty acid binding protein.
Topics: Circular Dichroism; Fatty Acid-Binding Proteins; Kinetics; Models, Molecular; Mutation; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Urea | 2005 |
Characterisation of disulfide-bond dynamics in non-native states of lysozyme and its disulfide deletion mutants by NMR.
Topics: Animals; Chickens; Disulfides; Hydrogen-Ion Concentration; Internet; Magnetic Resonance Spectroscopy; Models, Molecular; Muramidase; Mutation; Protein Denaturation; Protein Structure, Tertiary; Tryptophan; Urea; Water | 2005 |
Identification of pitfalls in the analysis of heat capacity changes of beta-lactoglobulin A.
Topics: Animals; Calorimetry; Calorimetry, Differential Scanning; Cattle; Circular Dichroism; Hot Temperature; Lactoglobulins; Milk; Protein Denaturation; Protein Folding; Sensitivity and Specificity; Spectrometry, Fluorescence; Temperature; Thermodynamics; Tryptophan; Ultraviolet Rays; Urea | 2005 |
Tryptophan 243 affects interprotein contacts, cofactor binding and stability in D-amino acid oxidase from Rhodotorula gracilis.
Topics: D-Amino-Acid Oxidase; Enzyme Activation; Enzyme Stability; Kinetics; Models, Molecular; Mutation; Protein Conformation; Protein Folding; Protein Structure, Tertiary; Rhodotorula; Static Electricity; Structure-Activity Relationship; Temperature; Time Factors; Tryptophan; Urea | 2006 |
Steady-state and time-resolved fluorescence studies of the intestinal fatty acid binding protein.
Topics: Acids; Crystallography, X-Ray; Fatty Acid-Binding Proteins; Models, Molecular; Mutation; Protein Folding; Protein Structure, Tertiary; Spectrometry, Fluorescence; Time Factors; Tryptophan; Urea | 2006 |
Reversible inactivation of alkaline phosphatase from Atlantic cod (Gadus morhua) in urea.
Topics: Alkaline Phosphatase; Anilino Naphthalenesulfonates; Animals; Circular Dichroism; Dimerization; Fluorescence; Gadus morhua; Protein Folding; Protein Structure, Quaternary; Protein Structure, Secondary; Protein Structure, Tertiary; Thermodynamics; Tryptophan; Urea | 2006 |
A residual structure in unfolded intestinal fatty acid binding protein consists of amino acids that are neighbors in the native state.
Topics: Amino Acids; Circular Dichroism; Fatty Acid-Binding Proteins; Fluorescence; Fluorine; Hydrophobic and Hydrophilic Interactions; Nuclear Magnetic Resonance, Biomolecular; Phenylalanine; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Recombinant Proteins; Tryptophan; Tyrosine; Urea | 2006 |
Understanding the mechanism of beta-hairpin folding via phi-value analysis.
Topics: Amino Acid Sequence; Amino Acid Substitution; Circular Dichroism; Hydrophobic and Hydrophilic Interactions; Kinetics; Molecular Sequence Data; Peptides; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Spectrophotometry, Infrared; Temperature; Thermodynamics; Tryptophan; Urea | 2006 |
Native state energetics of the Src SH2 domain: evidence for a partially structured state in the denatured ensemble.
Topics: Animals; Chickens; CSK Tyrosine-Protein Kinase; Deuterium Exchange Measurement; Kinetics; Protein Conformation; Protein Denaturation; Protein Folding; Protein-Tyrosine Kinases; Spectrometry, Fluorescence; src Homology Domains; src-Family Kinases; Thermodynamics; Tryptophan; Urea | 2006 |
Aminotryptophan-containing barstar: structure--function tradeoff in protein design and engineering with an expanded genetic code.
Topics: Bacterial Proteins; Circular Dichroism; Crystallography, X-Ray; Genetic Code; Kinetics; Models, Molecular; Protein Biosynthesis; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Temperature; Thermodynamics; Tryptophan; Urea | 2006 |
Influence of urea additives on micellar morphology/protein conformation.
Topics: Animals; Buffers; Cattle; Circular Dichroism; Dose-Response Relationship, Drug; Female; Hydrogen-Ion Concentration; Methylurea Compounds; Micelles; Protein Conformation; Protein Structure, Secondary; Serum Albumin, Bovine; Solubility; Spectrometry, Fluorescence; Thiourea; Tryptophan; Urea; Viscosity; Water | 2006 |
Probing folded and unfolded states of outer membrane protein a with steady-state and time-resolved tryptophan fluorescence.
Topics: Anisotropy; Bacterial Outer Membrane Proteins; Biophysics; Circular Dichroism; Dimyristoylphosphatidylcholine; Escherichia coli; Kinetics; Micelles; Molecular Conformation; Mutation; Protein Folding; Protein Structure, Tertiary; Spectrometry, Fluorescence; Tryptophan; Urea | 2006 |
Thermodynamic analysis of three state denaturation of Peanut Agglutinin.
Topics: Dose-Response Relationship, Drug; Hot Temperature; Hydrogen-Ion Concentration; Microscopy, Fluorescence; Models, Chemical; Models, Molecular; Models, Statistical; Peanut Agglutinin; Protein Denaturation; Spectrometry, Fluorescence; Temperature; Thermodynamics; Tryptophan; Urea | 2006 |
An on-pathway intermediate in the folding of a PDZ domain.
Topics: Amino Acid Motifs; Biochemistry; Dose-Response Relationship, Drug; Fluorescence Resonance Energy Transfer; Kinetics; Models, Chemical; Peptides; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Temperature; Thermodynamics; Tryptophan; Urea | 2007 |
Guanidinium chloride and urea denaturations of beta-lactoglobulin A at pH 2.0 and 25 degrees C: the equilibrium intermediate contains non-native structures (helix, tryptophan and hydrophobic patches).
Topics: Animals; Cattle; Circular Dichroism; Guanidine; Hydrogen-Ion Concentration; Kinetics; Lactoglobulins; Parasympathomimetics; Protein Denaturation; Protein Structure, Secondary; Spectrophotometry, Ultraviolet; Tryptophan; Urea | 2007 |
A tryptophan rotamer located in a polar environment probes pH-dependent conformational changes in bovine beta-lactoglobulin A.
Topics: Amino Acids; Animals; Binding Sites; Cattle; Hydrogen-Ion Concentration; Lactoglobulins; Models, Molecular; Protein Structure, Tertiary; Spectrometry, Fluorescence; Tryptophan; Urea | 2007 |
Comparison of C40/82A and P27A C40/82A barstar mutants using 19F NMR.
Topics: Bacterial Proteins; Dose-Response Relationship, Drug; Fluorine; Hydrophobic and Hydrophilic Interactions; Motion; Mutation; Nuclear Magnetic Resonance, Biomolecular; Phenylalanine; Proline; Protein Conformation; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Temperature; Tryptophan; Ultracentrifugation; Urea | 2007 |
Gold nanoparticle-based tool to study protein conformational variants: implications in hemoglobinopathy.
Topics: Animals; Cattle; Fluorescence; Gold; Hemoglobinopathies; Hemoglobins; Humans; Nanoparticles; Particle Size; Protein Conformation; Protein Folding; Serum Albumin, Bovine; Surface Plasmon Resonance; Tryptophan; Tyrosine; Urea | 2007 |
Destruction of long-range interactions by a single mutation in lysozyme.
Topics: Amino Acid Sequence; Cluster Analysis; Computer Simulation; Crystallization; Enzyme Stability; Hydrogen-Ion Concentration; Kinetics; Models, Molecular; Muramidase; Point Mutation; Pressure; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Static Electricity; Temperature; Tryptophan; Urea; Water | 2007 |
Biophysical characterization of structural properties and folding of interleukin-1 receptor antagonist.
Topics: Anilino Naphthalenesulfonates; Circular Dichroism; Fluorescent Dyes; Humans; Indicators and Reagents; Interleukin 1 Receptor Antagonist Protein; Kinetics; Magnetic Resonance Spectroscopy; Models, Molecular; Mutation; Protein Denaturation; Protein Folding; Thermodynamics; Tryptophan; Urea | 2007 |
Kinetics of salt-dependent unfolding of [2Fe-2S] ferredoxin of Halobacterium salinarum.
Topics: Bacterial Proteins; Circular Dichroism; Ferredoxins; Halobacterium salinarum; Hydrogen-Ion Concentration; Kinetics; Models, Chemical; Protein Denaturation; Protein Folding; Sodium Chloride; Spectrometry, Fluorescence; Tryptophan; Urea | 2007 |
Unfolding and refolding of bovine alpha-crystallin in urea and its chaperone activity.
Topics: alpha-Crystallins; Animals; Cattle; Hydrophobic and Hydrophilic Interactions; Molecular Chaperones; Protein Folding; Protein Structure, Quaternary; Structure-Activity Relationship; Tryptophan; Urea | 2007 |
Dynamic similarity of the unfolded states of proteins L and G.
Topics: Algorithms; Bacterial Proteins; Computer Simulation; Deuterium Exchange Measurement; DNA-Binding Proteins; Fluorescence Resonance Energy Transfer; Guanidine; Hydrogen Bonding; Hydrophobic and Hydrophilic Interactions; Models, Chemical; Models, Theoretical; Protein Conformation; Protein Denaturation; Protein Folding; Protein Renaturation; Proteins; Recombinant Fusion Proteins; Thermodynamics; Tryptophan; Urea | 2007 |
Tryptophan fluorescence reveals the presence of long-range interactions in the denatured state of ribonuclease Sa.
Topics: Acrylamide; Acrylamides; Amino Acid Sequence; Disulfides; Fluorescence; Iodides; Molecular Conformation; Molecular Sequence Data; Peptides; Proteins; Ribonucleases; Spectrometry, Fluorescence; Time Factors; Tryptophan; Urea | 2008 |
Peptide sequence and conformation strongly influence tryptophan fluorescence.
Topics: Acrylamides; Biophysics; Computational Biology; Guanidine; Iodides; Molecular Conformation; Normal Distribution; Peptides; Spectrometry, Fluorescence; Temperature; Time Factors; Tryptophan; Tyrosine; Urea | 2008 |
Different urea stoichiometries between the dissociation and denaturation of tobacco mosaic virus as probed by hydrostatic pressure.
Topics: Chromatography, Gel; Chromatography, High Pressure Liquid; Hydrostatic Pressure; Microscopy, Electron, Transmission; Molecular Conformation; Tobacco Mosaic Virus; Tryptophan; Urea; Water | 2008 |
Domain stabilities in protein kinase R (PKR): evidence for weak interdomain interactions.
Topics: Circular Dichroism; DNA; Enzyme Stability; Fluorescent Dyes; Kinetics; Models, Molecular; Protein Denaturation; Protein Kinases; Protein Renaturation; Protein Structure, Tertiary; Tryptophan; Urea | 2008 |
Volume and free energy of folding for troponin C C-domain: linkage to ion binding and N-domain interaction.
Topics: Calcium; Fluorescence; Hydrostatic Pressure; Magnesium; Magnetic Resonance Spectroscopy; Protein Binding; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Thermodynamics; Troponin C; Tryptophan; Urea | 2008 |
Experimental evidence for the existence of a stable half-barrel subdomain in the (beta/alpha)8-barrel fold.
Topics: Aldose-Ketose Isomerases; Circular Dichroism; Escherichia coli; Models, Molecular; Molecular Weight; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Proteins; Temperature; Transient Receptor Potential Channels; Tryptophan; Urea | 2008 |
Thermodynamics, kinetics, and salt dependence of folding of YopM, a large leucine-rich repeat protein.
Topics: Bacterial Outer Membrane Proteins; Calorimetry, Differential Scanning; Circular Dichroism; Kinetics; Leucine-Rich Repeat Proteins; Models, Molecular; Protein Folding; Protein Structure, Secondary; Proteins; Sodium Chloride; Solutions; Spectrometry, Fluorescence; Temperature; Thermodynamics; Tryptophan; Urea | 2008 |
Effects of tryptophan microenvironment, soluble domain, and vesicle size on the thermodynamics of membrane protein folding: lessons from the transmembrane protein OmpA.
Topics: Bacterial Outer Membrane Proteins; Models, Molecular; Mutation; Protein Conformation; Protein Folding; Protein Renaturation; Protein Stability; Protein Structure, Tertiary; Scattering, Radiation; Solubility; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Unilamellar Liposomes; Urea | 2008 |
Comparison of the folding mechanism of highly homologous proteins in the lipid-binding protein family.
Topics: Animals; Carrier Proteins; Humans; Hydroxysteroid Dehydrogenases; Kinetics; Membrane Glycoproteins; Models, Molecular; Nuclear Magnetic Resonance, Biomolecular; Protein Folding; Protein Structure, Secondary; Rats; Recombinant Proteins; Sequence Homology, Amino Acid; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Tyrosine; Urea | 2009 |
Hydrophobic core of the steroidogenic acute regulatory protein for cholesterol transport.
Topics: Anilino Naphthalenesulfonates; Animals; Biological Transport; Cholesterol; Crystallography, X-Ray; Hydrophobic and Hydrophilic Interactions; Kinetics; Mass Spectrometry; Mutant Proteins; Phosphoproteins; Pliability; Protein Denaturation; Protein Folding; Protein Processing, Post-Translational; Protein Stability; Protein Structure, Secondary; Spectrometry, Fluorescence; Structure-Activity Relationship; Sus scrofa; Trifluoroethanol; Tryptophan; Urea | 2009 |
Kinetics of contact formation and end-to-end distance distributions of swollen disordered peptides.
Topics: Algorithms; Buffers; Circular Dichroism; Cysteine; Energy Transfer; Guanidine; Kinetics; Models, Chemical; Peptides; Protein Conformation; Protein Denaturation; Tryptophan; Urea | 2009 |
Leishmania donovani trypanothione reductase: role of urea and guanidine hydrochloride in modulation of functional and structural properties.
Topics: Animals; Circular Dichroism; Dimerization; Enzyme Stability; Flavin-Adenine Dinucleotide; Guanidine; Leishmania donovani; Models, Molecular; NADH, NADPH Oxidoreductases; Protein Denaturation; Protein Folding; Protein Structure, Quaternary; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins; Spectrometry, Fluorescence; Spectrophotometry; Sulfhydryl Compounds; Thermodynamics; Tryptophan; Urea | 2009 |
[Tryptophan and its metabolites in renal allograft recipients].
Topics: Adult; Aged; Creatinine; Female; Humans; Kidney Failure, Chronic; Kidney Transplantation; Kynurenine; Kynurenine 3-Monooxygenase; Male; Middle Aged; Quinolinic Acid; Reference Values; Tryptophan; Urea | 2009 |
Denaturation and solvent effect on the conformation and fibril formation of TGFBIp.
Topics: Amyloid; Cell Line; Extracellular Matrix Proteins; Guanidine; Humans; Hydrogen-Ion Concentration; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Solvents; Spectrometry, Fluorescence; Transforming Growth Factor beta; Trifluoroethanol; Tryptophan; Urea | 2009 |
Buffer-dependent fragmentation of a humanized full-length monoclonal antibody.
Topics: Antibodies, Monoclonal; Buffers; Calorimetry, Differential Scanning; Electrophoresis, Polyacrylamide Gel; Humans; Immunoglobulin G; Protein Conformation; Protein Folding; Protein Stability; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Temperature; Tryptophan; Urea | 2010 |
Molecular mechanism of polyethylene glycol mediated stabilization of protein.
Topics: Animals; Cattle; Circular Dichroism; Polyethylene Glycols; Protein Binding; Protein Conformation; Protein Denaturation; Protein Stability; Serum Albumin, Bovine; Spectrometry, Fluorescence; Tryptophan; Urea | 2010 |
Thermodynamics of the Trp-cage miniprotein unfolding in urea.
Topics: Computer Simulation; Peptides; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Thermodynamics; Tryptophan; Urea | 2010 |
Differential scanning calorimetry and fluorescence study of lactoperoxidase as a function of guanidinium-HCl, urea, and pH.
Topics: Animals; Calorimetry; Calorimetry, Differential Scanning; Cattle; Guanidine; Hydrochloric Acid; Hydrogen-Ion Concentration; Kinetics; Lactoperoxidase; Microscopy, Fluorescence; Molecular Conformation; Temperature; Thermodynamics; Tryptophan; Urea | 2010 |
Residual structure in a peptide fragment of the outer membrane protein X under denaturing conditions: a molecular dynamics study.
Topics: Aspartic Acid; Bacterial Outer Membrane Proteins; Escherichia coli Proteins; Hydrolases; Hydrophobic and Hydrophilic Interactions; Magnetic Resonance Spectroscopy; Molecular Dynamics Simulation; Peptide Fragments; Protein Denaturation; Protein Structure, Secondary; Serine; Time Factors; Tryptophan; Tyrosine; Urea | 2010 |
Calcium-induced folding and stabilization of the intrinsically disordered RTX domain of the CyaA toxin.
Topics: Adenylate Cyclase Toxin; Bordetella pertussis; Calcium; Circular Dichroism; Fluorescence; Guanidine; Models, Molecular; Protein Folding; Protein Stability; Protein Structure, Tertiary; Sodium Chloride; Spectroscopy, Fourier Transform Infrared; Tryptophan; Urea | 2010 |
Urea and guanidinium induced denaturation of a Trp-cage miniprotein.
Topics: Calorimetry, Differential Scanning; Circular Dichroism; Guanidine; Magnetic Resonance Spectroscopy; Models, Molecular; Protein Denaturation; Tryptophan; Urea | 2011 |
Biophysical studies of an NAD(P)(+)-dependent aldehyde dehydrogenase from Bacillus licheniformis.
Topics: Aldehyde Dehydrogenase; Bacillus; Biophysical Phenomena; Circular Dichroism; Guanidine; Protein Multimerization; Protein Structure, Quaternary; Protein Unfolding; Sodium Dodecyl Sulfate; Solvents; Spectrometry, Fluorescence; Temperature; Tryptophan; Ultracentrifugation; Urea | 2011 |
Microsecond unfolding kinetics of sheep prion protein reveals an intermediate that correlates with susceptibility to classical scrapie.
Topics: Animals; Disease Susceptibility; Guanidine; Humans; Hydrogen-Ion Concentration; Kinetics; Models, Biological; Models, Molecular; Prions; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Unfolding; Scrapie; Sheep; Spectrometry, Fluorescence; Tryptophan; Urea | 2011 |
Influence of denaturants on native-state structural fluctuations in azurin probed by molecular size-dependent quenching of Trp phosphorescence.
Topics: Azurin; Guanidine; Protein Denaturation; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Urea | 2011 |
Absence of post-prandial gut anabolism after intake of a low quality protein meal.
Topics: Amino Acids; Animal Feed; Animal Nutritional Physiological Phenomena; Animals; Diet, Protein-Restricted; Dietary Proteins; Dietary Supplements; Digestion; Energy Intake; Female; Gastrointestinal Tract; Gelatin; Hybridization, Genetic; Liver; Models, Animal; Nitrogen; Postoperative Care; Postprandial Period; Swine; Tryptophan; Urea; Viscera | 2012 |
Folding dynamics of Trp-cage in the presence of chemical interference and macromolecular crowding. I.
Topics: Algorithms; Hydrophobic and Hydrophilic Interactions; Magnetic Resonance Spectroscopy; Molecular Dynamics Simulation; Peptides; Protein Folding; Protein Stability; Proteins; Tryptophan; Urea | 2011 |
S-Carboxyethylcysteine (a constituent of Acacia seed) negatively affects casein protein utilization by rats.
Topics: Acacia; Amino Acids; Animals; Carbocysteine; Caseins; Cysteine; Disasters; Food, Formulated; Humans; Methionine; Niger; Nutritive Value; Rats; Rats, Wistar; Seeds; Starvation; Tryptophan; Urea; Weaning; Weight Gain | 2012 |
Structural changes of soy proteins at the oil-water interface studied by fluorescence spectroscopy.
Topics: Adsorption; Antigens, Plant; Calorimetry; Emulsions; Globulins; Glycine max; Hydrophobic and Hydrophilic Interactions; Oils; Protein Conformation; Protein Denaturation; Seed Storage Proteins; Soybean Proteins; Spectrometry, Fluorescence; Temperature; Tryptophan; Urea; Water | 2012 |
Mutational analysis of cysteine 328 and cysteine 368 at the interface of Plasmodium falciparum adenylosuccinate synthetase.
Topics: Adenylosuccinate Synthase; Amino Acid Substitution; Chromatography, Gel; Copper; Cysteine; Enzyme Stability; Iodoacetic Acid; Kinetics; Manganese; Models, Molecular; Mutagenesis, Site-Directed; Plasmodium falciparum; Protein Denaturation; Protein Folding; Protein Interaction Domains and Motifs; Protein Structure, Quaternary; Protein Structure, Secondary; Protein Subunits; Protozoan Proteins; Tryptophan; Urea | 2012 |
Study on effect of lipophilic curcumin on sub-domain IIA site of human serum albumin during unfolded and refolded states: a synchronous fluorescence spectroscopic study.
Topics: Antioxidants; Binding Sites; Curcumin; Humans; Hydrophobic and Hydrophilic Interactions; Kinetics; Models, Molecular; Protein Binding; Protein Folding; Protein Structure, Tertiary; Serum Albumin; Spectrometry, Fluorescence; Tryptophan; Tyrosine; Urea | 2012 |
Characterization of molten globule PopB in absence and presence of its chaperone PcrH.
Topics: Amino Acid Sequence; Bacterial Proteins; Fluorescence; Molecular Chaperones; Molecular Sequence Data; Protein Denaturation; Protein Interaction Mapping; Protein Stability; Protein Structure, Secondary; Protein Unfolding; Pseudomonas aeruginosa; Pseudomonas Infections; Tryptophan; Urea | 2012 |
Differing structural characteristics of molten globule intermediate of peanut lectin in urea and guanidine-HCl.
Topics: Acrylamide; Anilino Naphthalenesulfonates; Bromosuccinimide; Guanidine; Peanut Agglutinin; Protein Binding; Protein Denaturation; Protein Structure, Tertiary; Protein Unfolding; Temperature; Tryptophan; Urea | 2012 |
Native and denatured forms of proteins can be discriminated at edge plane carbon electrodes.
Topics: alpha-Synuclein; Animals; Cattle; Chickens; Electrochemical Techniques; Electrodes; Fructose-Bisphosphate Aldolase; Graphite; Humans; Protein Denaturation; Proteins; Serum Albumin; Tryptophan; Tyrosine; Urea | 2012 |
Indole peroxygenase activity of indoleamine 2,3-dioxygenase.
Topics: Animals; Aspergillus niger; Carbamide Peroxide; Cattle; Escherichia coli; Esterases; Free Radical Scavengers; Glucose Oxidase; Humans; Hydrogen Peroxide; Indoleamine-Pyrrole 2,3,-Dioxygenase; Indoles; Mixed Function Oxygenases; Models, Chemical; Oxidation-Reduction; Oxygen Isotopes; Peroxides; Superoxide Dismutase; Swine; Tryptophan; Urea | 2012 |
Application of second-derivative fluorescence spectroscopy to monitor subtle changes in a monoclonal antibody structure.
Topics: Antibodies, Monoclonal; Buffers; Ethylene Glycol; Hydrogen-Ion Concentration; Polymers; Protein Denaturation; Protein Stability; Protein Structure, Tertiary; Protein Unfolding; Solvents; Spectrometry, Fluorescence; Sucrose; Temperature; Tryptophan; Urea | 2013 |
Slow, reversible, coupled folding and binding of the spectrin tetramerization domain.
Topics: Fluorescence; Humans; Kinetics; Protein Denaturation; Protein Folding; Protein Multimerization; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrin; Tryptophan; Urea | 2012 |
Monomeric banana lectin at acidic pH overrules conformational stability of its native dimeric form.
Topics: Acids; Acrylamide; Anilino Naphthalenesulfonates; Chromatography, Gel; Circular Dichroism; Hydrodynamics; Hydrogen-Ion Concentration; Kinetics; Lectins; Light; Musa; Protein Binding; Protein Denaturation; Protein Multimerization; Protein Stability; Protein Structure, Secondary; Protein Structure, Tertiary; Protein Unfolding; Scattering, Radiation; Software; Spectrometry, Fluorescence; Temperature; Tryptophan; Urea | 2013 |
Nonuniform chain collapse during early stages of staphylococcal nuclease folding detected by fluorescence resonance energy transfer and ultrarapid mixing methods.
Topics: Circular Dichroism; Cysteine; Energy Transfer; Fluorescence Resonance Energy Transfer; Kinetics; Micrococcal Nuclease; Models, Molecular; Mutation, Missense; Protein Folding; Protein Structure, Tertiary; Tryptophan; Urea | 2013 |
Thermally stable harpin, HrpZPss is sensitive to chemical denaturants: probing tryptophan environment, chemical and thermal unfolding by fluorescence spectroscopy.
Topics: Acrylamide; Bacterial Outer Membrane Proteins; Calorimetry, Differential Scanning; Guanidine; Kinetics; Protein Denaturation; Protein Stability; Protein Unfolding; Spectrometry, Fluorescence; Tryptophan; Urea | 2013 |
Unfolding study of a trimeric membrane protein AcrB.
Topics: Circular Dichroism; Escherichia coli; Escherichia coli Proteins; Kinetics; Models, Molecular; Multidrug Resistance-Associated Proteins; Mutation; Phenylalanine; Protein Denaturation; Protein Multimerization; Protein Refolding; Protein Structure, Secondary; Protein Structure, Tertiary; Sodium Dodecyl Sulfate; Tryptophan; Urea | 2014 |
Equilibrium folding of pro-HlyA from Escherichia coli reveals a stable calcium ion dependent folding intermediate.
Topics: Bacterial Secretion Systems; Calcium; Escherichia coli; Escherichia coli Proteins; Gene Expression; Hemolysin Proteins; Kinetics; Mutation; Protein Binding; Protein Folding; Protein Precursors; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Fluorescence; Thermodynamics; Tryptophan; Urea | 2014 |
Chemical- and thermal-induced unfolding of Leishmania donovani ribose-5-phosphate isomerase B: a single-tryptophan protein.
Topics: Aldose-Ketose Isomerases; Enzyme Stability; Guanidine; Leishmania donovani; Protein Unfolding; Recombinant Proteins; Temperature; Tryptophan; Urea | 2014 |
Organization and dynamics of tryptophan residues in brain spectrin: novel insight into conformational flexibility.
Topics: 2-Naphthylamine; Animals; Binding Sites; Brain; Circular Dichroism; Fluorescence; Hydrophobic and Hydrophilic Interactions; Protein Conformation; Protein Denaturation; Sheep; Spectrin; Spectrometry, Fluorescence; Tryptophan; Urea | 2015 |
Milk protein yield and mammary metabolism are affected by phenylalanine deficiency but not by threonine or tryptophan deficiency.
Topics: 3-Hydroxybutyric Acid; Animals; Arteries; Cattle; Female; Lactation; Mammary Glands, Animal; Milk; Milk Proteins; Phenylalanine; Threonine; Tryptophan; Urea | 2016 |
Metabolomic signatures of drug response phenotypes for ketamine and esketamine in subjects with refractory major depressive disorder: new mechanistic insights for rapid acting antidepressants.
Topics: Adult; Arginine; Citrulline; Depressive Disorder, Major; Depressive Disorder, Treatment-Resistant; Excitatory Amino Acid Antagonists; Female; Glutamic Acid; Humans; Indoleacetic Acids; Infusions, Intravenous; Ketamine; Male; Metabolomics; Methionine; Middle Aged; Ornithine; Phenotype; Phospholipids; Tryptophan; Urea | 2016 |
Glossoscolex paulistus hemoglobin with fluorescein isothiocyanate: Steady-state and time-resolved fluorescence.
Topics: Animals; Fluorescein-5-isothiocyanate; Fluorescence; Hemoglobins; Oligochaeta; Protein Denaturation; Tryptophan; Urea | 2017 |
Effect of pH on stability, conformation, and chaperone activity of erythroid & non-erythroid spectrin.
Topics: Circular Dichroism; Hydrogen-Ion Concentration; Molecular Chaperones; Protein Conformation; Protein Stability; Spectrin; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Tryptophan; Urea | 2017 |
Fluorescence and circular dichroism studies on the accessibility of tryptophan residues and unfolding of a jacalin-related α-d-galactose-specific lectin from mulberry (Morus indica).
Topics: Cesium; Circular Dichroism; Galactose; Guanidine; Guanidines; Hydrogen-Ion Concentration; Iodides; Lectins; Morus; Plant Lectins; Protein Structure, Quaternary; Protein Structure, Secondary; Protein Unfolding; Spectrometry, Fluorescence; Temperature; Thiocyanates; Tryptophan; Urea | 2017 |
pH-Dependent Binding of Chloride to a Marine Alkaline Phosphatase Affects the Catalysis, Active Site Stability, and Dimer Equilibrium.
Topics: Alkaline Phosphatase; Aquatic Organisms; Bacterial Proteins; Catalysis; Catalytic Domain; Chlorides; Enzyme Activation; Enzyme Stability; Fluorescence; Hydrogen-Ion Concentration; Magnesium; Osmolar Concentration; Phosphorylation; Protein Denaturation; Protein Multimerization; Tryptophan; Urea; Vibrio; Zinc | 2017 |
Fluorotryptophan Incorporation Modulates the Structure and Stability of Transthyretin in a Site-Specific Manner.
Topics: Amino Acid Substitution; Dimerization; Fluorescent Dyes; Kinetics; Models, Molecular; Mutation; Nuclear Magnetic Resonance, Biomolecular; Prealbumin; Protein Aggregation, Pathological; Protein Conformation; Protein Denaturation; Protein Interaction Domains and Motifs; Protein Multimerization; Protein Stability; Protein Unfolding; Recombinant Proteins; Tryptophan; Urea | 2017 |
Metabolomic analysis of oxidative stress: Superoxide dismutase mutation and paraquat induced stress in Drosophila melanogaster.
Topics: Amines; Animals; Animals, Genetically Modified; Carboxylic Acids; Chromatography, Liquid; Drosophila melanogaster; Isotope Labeling; Male; Metabolic Networks and Pathways; Metabolome; Mutation; Oxidative Stress; Paraquat; Phenols; Porphyrins; Principal Component Analysis; Superoxide Dismutase; Tandem Mass Spectrometry; Tryptophan; Urea | 2017 |
Low-Protein Diets Decrease Porcine Nitrogen Excretion but with Restrictive Effects on Amino Acid Utilization.
Topics: Amino Acids; Animals; Diet; Diet, Protein-Restricted; Dietary Supplements; Feces; Liver; Lysine; Male; Methionine; Nitrogen; Sus scrofa; Threonine; Tryptophan; Urea; Viscera | 2018 |
Role of the glutaredoxin domain and FAD in the stabilization of thioredoxin glutathione reductase.
Topics: Animals; Catalysis; Dithionitrobenzoic Acid; Fasciola; Flavin-Adenine Dinucleotide; Glutaredoxins; Helminth Proteins; Multienzyme Complexes; Mutation; NADH, NADPH Oxidoreductases; NADP; Protein Binding; Protein Conformation; Protein Domains; Protein Stability; Protein Unfolding; Thioredoxins; Tryptophan; Urea | 2018 |
A Non-Targeted LC-MS Profiling Reveals Elevated Levels of Carnitine Precursors and Trimethylated Compounds in the Cord Plasma of Pre-Eclamptic Infants.
Topics: Adult; Biomarkers; Carnitine; Case-Control Studies; Chromatography, Liquid; Creatine; Cross-Sectional Studies; Female; Fetal Blood; Humans; Infant, Newborn; Metabolomics; Phosphatidylcholines; Pre-Eclampsia; Pregnancy; Principal Component Analysis; Putrescine; Pyridoxic Acid; Tandem Mass Spectrometry; Tryptophan; Urea | 2018 |
High-resolution structures of inhibitor complexes of human indoleamine 2,3-dioxygenase 1 in a new crystal form.
Topics: Catalytic Domain; Crystallization; Crystallography, X-Ray; Enzyme Inhibitors; Heme; Humans; Indoleamine-Pyrrole 2,3,-Dioxygenase; Mutation; Tryptophan; Urea | 2018 |
Experimental approach to study the effect of mutations on the protein folding pathway.
Topics: Amino Acid Substitution; Animals; Carbonic Anhydrase II; Cattle; Disulfides; Models, Molecular; Mutation; Protein Conformation; Protein Denaturation; Protein Folding; Protein Stability; Recombinant Proteins; Spectrometry, Fluorescence; Tryptophan; Unfolded Protein Response; Urea | 2019 |
Imidazolo and tryptophan-imidazolo hybrid derived ureas/thioureas as potent bioactive agents - SAR and molecular modelling studies.
Topics: Anti-Bacterial Agents; Anti-Inflammatory Agents, Non-Steroidal; Antifungal Agents; Antioxidants; Dose-Response Relationship, Drug; Humans; Imidazoles; Microbial Sensitivity Tests; Models, Molecular; Molecular Structure; Structure-Activity Relationship; Tryptophan; Urea | 2019 |
An insight into the folding and stability of Arabidopsis thaliana SOG1 transcription factor under salinity stress in vitro.
Topics: Arabidopsis; Arabidopsis Proteins; Binding Sites; Circular Dichroism; DNA Damage; DNA, Plant; Gene Deletion; Gene Expression Regulation, Plant; Light; Protein Binding; Protein Domains; Protein Folding; Recombinant Proteins; Salt Stress; Scattering, Radiation; Surface Properties; Transcription Factors; Tryptophan; Urea | 2019 |
Fluorescence Methods Applied to the Description of Urea-Dependent YME1L Protease Unfolding.
Topics: Anilino Naphthalenesulfonates; Fluorescence; Nucleotides; Peptide Hydrolases; Protein Denaturation; Protein Unfolding; Solvents; Tryptophan; Urea | 2020 |
Serotonin level as a potent diabetes biomarker based on electrochemical sensing: a new approach in a zebra fish model.
Topics: Animals; Ascorbic Acid; Biomarkers; Diabetes Mellitus; Dopamine; Electrochemical Techniques; Electrodes; Glucose; Gold; Hydrogen Bonding; Limit of Detection; Metal Nanoparticles; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Nanocomposites; Particle Size; Reproducibility of Results; Serotonin; Tryptophan; Urea; Zebrafish | 2021 |
Nitric Oxide-Releasing Tryptophan-Based Poly(ester urea)s Electrospun Composite Nanofiber Mats with Antibacterial and Antibiofilm Activities for Infected Wound Healing.
Topics: Anti-Bacterial Agents; Biofilms; Esters; Humans; Nanofibers; Nitric Oxide; Tryptophan; Urea; Wound Healing; Wound Infection | 2022 |
The Denaturant- and Mutation-Induced Disassembly of
Topics: Circular Dichroism; Mutation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Pseudomonas aeruginosa; Thermodynamics; Tryptophan; Urea | 2022 |