urea has been researched along with sepharose in 47 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 24 (51.06) | 18.7374 |
| 1990's | 10 (21.28) | 18.2507 |
| 2000's | 9 (19.15) | 29.6817 |
| 2010's | 3 (6.38) | 24.3611 |
| 2020's | 1 (2.13) | 2.80 |
| Authors | Studies |
|---|---|
| Ishii, S; Kasai, K | 1 |
| Montgomery, R; Sheperd, V | 1 |
| Greenwood, C; Moore, TA | 1 |
| Brunner, G; Harstick, K; Holloway, CJ; Külpmann, WR; Petry, K | 1 |
| Anderson, PA; Daniel, TM | 1 |
| Andersson, L; Mosbach, K | 1 |
| Andrews, P | 1 |
| Chan, WW; Mosbach, K | 1 |
| Werber, MM | 1 |
| Joklik, WK; Mitchell, WR; Schuerch, AR | 1 |
| Walter, B | 1 |
| Kishi, K; Nadano, D; Takeshita, H; Tenjo, E; Yasuda, T | 1 |
| Du, B; Falcone, DJ; McCaffrey, TA | 1 |
| Danev, SI; Dimitrov, DG; Ivanov, IP; Palashev, ChD | 1 |
| Heiner, DC; Kim, JS; Kim, KS; Short, JA; Wass, CA | 1 |
| Amos, B; Barnes, DW; Reing, JE | 1 |
| Reddy, LG; Shankar, V | 1 |
| Goldschmidt-Clermont, PJ; Kaiser, DA; Levine, BA; Pollard, TD | 1 |
| Elanogovan, N; Hoffman, WL; Jump, AA; Kelly, PJ | 1 |
| Schlaepfer, WW; Zimmerman, UJ | 1 |
| Wille, LE | 1 |
| Irie, M; Nishimura, T; Ogi, K | 1 |
| Bergquist, PL; Langridge, J; Langridge, P | 1 |
| Poliquin, L; Shore, GC | 1 |
| Abrudan, I; Bârzu, O; Lascu, I; Matinca, D; Mureşan, L; Oargă, M; Porumb, H; Presecan, E; Vancea, D | 1 |
| Stromska, DP | 1 |
| Afting, EG; Recker, ML | 1 |
| Delers, F; Domingo, M; Lombart, C; Musquera, S | 1 |
| Hayashi, T; Mizuno, K | 1 |
| Amemiya, H; Enosawa, S; Kakefuda, T; Suzuki, S | 1 |
| Fountoulakis, M; Juranville, JF | 1 |
| Kishore, U; Lopez Bernal, A; Morgan, C; Reid, KB; Singh, M; Strong, P | 1 |
| Szabó, G; Szilágyi, I; Varga, T | 1 |
| Rutkowska, SM; Skowron, PM | 1 |
| Fuchs, H; Gessner, R; Tauber, R | 1 |
| Ito, M; Kato, Y; Nagata, K; Oda, Y; Suzuki, K; Tanokura, M; Yamagoe, S | 1 |
| Batista-Viera, F; Betancor, L; Cuadra, K; Grazú, V; Manta, C; Ovsejevi, K | 1 |
| Dodd, JA; Nomura, M; Tongaonkar, P | 1 |
| Leon, O; Roth, MJ; Sengupta, S; Snyder, C; Zúñiga, R | 1 |
| Fu, XT; Kim, SM; Lin, H | 1 |
| Cabrera, R; Fernandez-Lahore, M; Galvis, L; Zhelyazkova, P | 1 |
| Leuba, SH; Rodriguez-Collazo, P; Zlatanova, J | 1 |
| Dalal, M; Dinges, R; Field, MJ; Garnick, S; Gunson, J; Kaleas, KA; Khoo, S; Laird, MW; Lee, M; Moiseff, K; Pizarro, SA; Reilly, DE; Schmelzer, CH | 1 |
| Eppink, M; Freydell, EJ; Ottens, M; van der Wielen, L | 1 |
| Ashok, V; Gupta, T; Joshi, P; Suchitra, S | 1 |
| Karahanian, E; Leuba, S; Rodriguez-Collazo, P; Zlatanova, J | 1 |
| Almeida, IF; Bastos, V; Correia-Sá, I; Costa, PC; Dias-Pereira, P; Fonseca, DFS; Freire, CSR; Oliveira, H; Silvestre, AJD; Vilela, C | 1 |
47 other study(ies) available for urea and sepharose
| Article | Year |
|---|---|
Affinity chromatography of trypsin and related enzymes. I. Preparation and characteristics of an affinity adsorbent containing tryptic peptides from protamine as ligands.
Topics: Amino Acids; Benzamidines; Chromatography, Affinity; Chymotrypsin; Hydrogen-Ion Concentration; Ligands; Oligopeptides; Osmolar Concentration; Protamines; Sepharose; Streptomyces griseus; Temperature; Trypsin; Trypsinogen; Urea | 1975 |
Alpha-D-Mannosidase. Preparation and properties of free and insolubilized enzyme.
Topics: Disaccharidases; Hot Temperature; Hydrogen-Ion Concentration; Macromolecular Substances; Mannosidases; Molecular Weight; Ovalbumin; Plants; Protein Binding; Sepharose; Urea | 1976 |
An investigation of protein conformation of cytochrome c by using cytochrome c insolubilized on to agarose gel.
Topics: Animals; Cytochrome c Group; Horses; Hydrogen-Ion Concentration; Myocardium; Osmolar Concentration; Oxidation-Reduction; Protein Conformation; Protein Denaturation; Sepharose; Spectrum Analysis; Urea | 1976 |
The removal of hypnotic drugs from human serum. A comparative investigation of the adsorptive properties of native and agarose-encapsulated resins and charcoal.
Topics: Adsorption; Biocompatible Materials; Capsules; Charcoal; Glutethimide; Hemoperfusion; Humans; Hypnotics and Sedatives; Ion Exchange Resins; Mathematics; Methaqualone; Phenobarbital; Polysaccharides; Sepharose; Urea | 1979 |
The use of immunoabsorbents for the purification of mycobacterial antigens.
Topics: Animals; Antigen-Antibody Complex; Antigens, Bacterial; Chromatography, Affinity; gamma-Globulins; Goats; Hydrogen-Ion Concentration; Immunosorbents; Ligands; Mycobacterium tuberculosis; Sepharose; Time Factors; Urea | 1977 |
The use of biochemical solid-phase techniques in the study of alcohol dehydrogenase. 1. Some studies on subunit interactions in alcohol dehydrogenase with subunits covalently bound to agarose.
Topics: Alcohol Oxidoreductases; Animals; Enzymes, Immobilized; Horses; Isoenzymes; Liver; Macromolecular Substances; Sepharose; Urea | 1979 |
Possible means of improving yields in protein chromatography.
Topics: Absorption; Chromatography; Chromatography, Affinity; Chromatography, Gel; Lactalbumin; Lactose Synthase; Proteins; Sepharose; Urea | 1975 |
Effects of subunit interactions on the activity of lactate dehydrogenase studied in immobilized enzyme systems.
Topics: Animals; Isoenzymes; Kinetics; L-Lactate Dehydrogenase; Macromolecular Substances; Muscles; Rabbits; Sepharose; Solubility; Urea | 1976 |
Nucleophilicity of isourea linkages in substituted agaroses. A direct method for the determination of alkylamino groups coupled to CNBr-activated agarose.
Topics: Amines; Binding Sites; Chemical Phenomena; Chemistry; Chromatography, Affinity; Cyanogen Bromide; Kinetics; Methods; Polysaccharides; Sepharose; Urea | 1976 |
Isolation of intact individual species of single- and double-stranded RNA after fractionation by polyacrylamide gel electrophoresis.
Topics: Centrifugation, Density Gradient; Electrophoresis, Polyacrylamide Gel; Ethidium; Indicators and Reagents; Methods; Molecular Weight; Reoviridae; RNA, Viral; Sepharose; Urea | 1975 |
Characterization of agarose-bound trypsin.
Topics: Caseins; Polysaccharides; Protein Binding; Protein Denaturation; Sepharose; Temperature; Trypsin; Urea | 1976 |
The zymogram method for detection of ribonucleases after isoelectric focusing: analysis of multiple forms of human, bovine, and microbial enzymes.
Topics: Acrylic Resins; Animals; Aspergillus oryzae; Cattle; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Ethidium; Fluorescence; Humans; Hydrolysis; Isoelectric Focusing; Isoenzymes; Pancreas; Ribonucleases; RNA; Sepharose; Snakes; Species Specificity; Urea | 1992 |
Transforming growth factor-beta 1 is a heparin-binding protein: identification of putative heparin-binding regions and isolation of heparins with varying affinity for TGF-beta 1.
Topics: Amino Acid Sequence; Binding, Competitive; Heparin; Molecular Sequence Data; Peptides; Sepharose; Transforming Growth Factor beta; Urea | 1992 |
[A method for determining urea by using immobilized urease].
Topics: Enzymes, Immobilized; Humans; Hydrolysis; Indicators and Reagents; Kidney Diseases; Phenol; Phenols; Reproducibility of Results; Sepharose; Urea; Urease | 1990 |
A human IgG 3 is opsonic in vitro against type III group B streptococci.
Topics: Humans; Hydrogen-Ion Concentration; Immunoglobulin G; Opsonin Proteins; Sepharose; Streptococcus agalactiae; Urea | 1990 |
Heparin-binding properties of human serum spreading factor.
Topics: Blood Proteins; Glycoproteins; Heparin; Humans; Hydrogen-Ion Concentration; Kinetics; Osmolar Concentration; Protein Binding; Sepharose; Urea; Vitronectin | 1985 |
Preparation and properties of RNase T2 immobilized on concanavalin A-sepharose.
Topics: Aspergillus oryzae; Endoribonucleases; Enzyme Stability; Enzymes, Immobilized; Hydrogen-Ion Concentration; Kinetics; Metals; Sepharose; Solubility; Temperature; Urea | 1989 |
Characterization of renatured profilin purified by urea elution from poly-L-proline agarose columns.
Topics: Acanthamoeba; Animals; Antibodies, Monoclonal; Blood Platelets; Chromatography, Affinity; Contractile Proteins; Dictyostelium; Humans; Immunochemistry; Magnetic Resonance Spectroscopy; Microfilament Proteins; Peptides; Profilins; Sepharose; Urea | 1989 |
Rehydratable agarose gels: application to isoelectric focusing in 9 molar urea.
Topics: Acrylic Resins; Humans; Immunoelectrophoresis; Immunoglobulin G; Immunoglobulin M; Isoelectric Focusing; Sepharose; Solutions; Urea | 1989 |
Calcium-activated neutral proteases (calpains) are carbohydrate binding proteins.
Topics: Calcium; Calcium-Binding Proteins; Calpain; Carbohydrate Metabolism; Chromatography; Disaccharides; Enzyme Activation; Ethylene Glycol; Ethylene Glycols; Leupeptins; Octoxynol; Polyethylene Glycols; Sepharose; Thiocyanates; Urea | 1988 |
Further characterization of the serum pre-alpha-lipoprotein.
Topics: Apoproteins; Binding Sites; Blood Protein Electrophoresis; Cellulose; Chromatography, Affinity; Dextrans; Humans; Immunoelectrophoresis; Lipoproteins; Lipoproteins, HDL; Lipoproteins, LDL; Lipoproteins, VLDL; Protein Binding; Sepharose; Tromethamine; Urea | 1974 |
Some properties of ribonucleases from Aspergillus saitoi and seminal vesicles immobilized on sepharose 4B activated by cyanogen bromide.
Topics: Aspergillus; Cyanogen Bromide; Hydrogen-Ion Concentration; Kinetics; Male; Protein Binding; Ribonucleases; Seminal Vesicles; Sepharose; Urea | 1974 |
Extraction of nucleic acids from agarose gels.
Topics: DNA, Bacterial; Electrophoresis, Agar Gel; Escherichia coli; Methods; Plasmids; Polysaccharides; RNA, Bacterial; RNA, Ribosomal; Sepharose; Shigella sonnei; Urea | 1980 |
A method for efficient and selective recovery of membrane glycoproteins from concanavalin A-Sepharose using media containing sodium dodecyl sulfate and urea.
Topics: Animals; Cell Line; Chromatography, Affinity; Concanavalin A; Cricetinae; Glycoproteins; Indicators and Reagents; Kidney; Membrane Proteins; Sepharose; Simplexvirus; Sodium Dodecyl Sulfate; Urea; Viral Proteins | 1980 |
Catalytic properties of Sepharose-bound L-alanine dehydrogenase from Bacillus cereus.
Topics: Alanine Dehydrogenase; Amino Acid Oxidoreductases; Bacillus cereus; Binding Sites; Enzymes, Immobilized; Kinetics; Protein Denaturation; Sepharose; Solubility; Urea | 1983 |
Isoelectric focusing of tubulin in a new type of charge-balanced agarose.
Topics: Animals; Brain Chemistry; Female; Hydrogen-Ion Concentration; Isoelectric Focusing; Polysaccharides; Rats; Rats, Inbred Strains; Sepharose; Tubulin; Urea | 1982 |
Two-step affinity-chromatographic purification of cathepsin D from pig myometrium with high yield.
Topics: Animals; Cathepsin D; Cathepsins; Chromatography, Affinity; Concanavalin A; Electrophoresis, Polyacrylamide Gel; Female; Myometrium; Pepstatins; Sepharose; Swine; Urea; Uterus | 1981 |
A novel and specific method for the purification of hemoglobin-binding proteins.
Topics: Animals; Chickens; Chromatography, Affinity; Haptoglobins; Humans; Immunochemistry; Rabbits; Rats; Sepharose; Urea | 1981 |
Peculiar effect of urea on the interaction of type I collagen with heparin on chromatography.
Topics: Chromatography; Collagen; Drug Interactions; Heparin; Plant Proteins; Plants; Protein Denaturation; Sepharose; Urea | 1994 |
Ammonia removal activity of agarose-encapsulated rat hepatocytes.
Topics: Ammonia; Animals; Capsules; In Vitro Techniques; Kinetics; Liver; Male; Rats; Rats, Wistar; Sepharose; Time Factors; Urea | 1995 |
Reduced binding capacity of concanavalin A-sepharose after treatment with chaotropic agents.
Topics: Animals; Buffers; Chromatography, Affinity; Guanidine; Guanidines; Interferon-gamma; Mice; Octoxynol; Protein Denaturation; Sepharose; Sodium Dodecyl Sulfate; Urea | 1993 |
A novel method of purifying lung surfactant proteins A and D from the lung lavage of alveolar proteinosis patients and from pooled amniotic fluid.
Topics: Amniotic Fluid; Aspergillus fumigatus; Bronchoalveolar Lavage Fluid; Centrifugation; Chlorides; Chromatography, Affinity; Chromatography, Gel; Chromatography, Liquid; Edetic Acid; Electrophoresis, Polyacrylamide Gel; Glycoproteins; Humans; Leukocytes, Mononuclear; Manganese Compounds; Proteolipids; Pulmonary Alveolar Proteinosis; Pulmonary Surfactant-Associated Protein A; Pulmonary Surfactant-Associated Protein D; Pulmonary Surfactant-Associated Proteins; Pulmonary Surfactants; Sepharose; Urea | 1998 |
Single-strand breaks in agarose-embedded chromatin of nonapoptotic cells.
Topics: Animals; Cell Line; Chromatin; DNA Damage; DNA Fragmentation; DNA, Single-Stranded; Electrophoresis, Agar Gel; Electrophoresis, Gel, Pulsed-Field; Hot Temperature; Humans; Mice; Nucleic Acid Denaturation; Saccharomyces cerevisiae; Sepharose; Single-Strand Specific DNA and RNA Endonucleases; Urea | 1999 |
Rapid screening of elution conditions prior to immunopurification of proteins.
Topics: Antibodies, Monoclonal; Chlorella; Chromatography, Affinity; Deoxyribonucleases, Type II Site-Specific; Guanidine; Immunosorbents; Phycodnaviridae; Sepharose; Urea | 1999 |
Determination of optimal non-denaturing elution conditions from affinity columns by a solid-phase screen.
Topics: Apoproteins; Buffers; Chromatography, Affinity; Drug Stability; Ferric Compounds; Humans; Indicators and Reagents; Microchemistry; Receptors, Transferrin; Sepharose; Thiocyanates; Transferrin; Urea | 2001 |
Expression, oxidative refolding, and characterization of six-histidine-tagged recombinant human LECT2, a 16-kDa chemotactic protein with three disulfide bonds.
Topics: Animals; CHO Cells; Circular Dichroism; Cricetinae; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Humans; Intercellular Signaling Peptides and Proteins; Magnetic Resonance Spectroscopy; Oxidation-Reduction; Oxygen; Protein Binding; Protein Conformation; Protein Folding; Proteins; Recombinant Proteins; Sepharose; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrophotometry; Time Factors; Urea | 2003 |
Solid-phase reducing agents as alternative for reducing disulfide bonds in proteins.
Topics: Aspergillus oryzae; beta-Galactosidase; Chromatography, Gel; Disulfides; Dithiothreitol; Hydrogen-Ion Concentration; Kinetics; Kluyveromyces; Lactoglobulins; Models, Chemical; Sepharose; Sulfhydryl Compounds; Temperature; Urea | 2003 |
Purification and assay of upstream activation factor, core factor, Rrn3p, and yeast RNA polymerase I.
Topics: Biochemistry; DNA-Binding Proteins; Electrophoresis; Epitopes; Escherichia coli; Mutation; Plasmids; Pol1 Transcription Initiation Complex Proteins; Promoter Regions, Genetic; RNA Polymerase I; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Sepharose; Transcription Factors; Transcription, Genetic; Urea | 2003 |
Expression of the C-terminus of HIV-1 reverse transcriptase p66 and p51 subunits as a single polypeptide with RNase H activity.
Topics: Blotting, Western; Chromatography, Gel; Electrophoresis, Polyacrylamide Gel; Escherichia coli; HIV Reverse Transcriptase; Manganese; Models, Molecular; Peptides; Protein Binding; Protein Engineering; Protein Structure, Tertiary; Ribonuclease H; RNA, Transfer; Sepharose; Software; Urea | 2004 |
Purification and characterization of a novel beta-agarase, AgaA34, from Agarivorans albus YKW-34.
Topics: Alteromonadaceae; Animals; Chromatography, Gel; Chromatography, Ion Exchange; Disaccharides; Dithiothreitol; DNA, Bacterial; Enzyme Activators; Enzyme Inhibitors; Enzyme Stability; Galactosides; Gastropoda; Glycoside Hydrolases; Hydrogen-Ion Concentration; Kinetics; Magnetic Resonance Spectroscopy; Mercaptoethanol; Molecular Sequence Data; Molecular Weight; Oligosaccharides; Sepharose; Sequence Analysis, DNA; Sequence Analysis, Protein; Sequence Homology, Amino Acid; Sodium Dodecyl Sulfate; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Temperature; Urea | 2008 |
Tailoring orthogonal proteomic routines to understand protein separation during ion exchange chromatography.
Topics: Animals; Cattle; Chromatography, Ion Exchange; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Polyacrylamide Gel; Isoelectric Point; Plant Proteins; Protein Denaturation; Proteins; Proteomics; Sepharose; Serum Albumin, Bovine; Urea | 2008 |
Robust methods for purification of histones from cultured mammalian cells with the preservation of their native modifications.
Topics: Acetylation; Animals; Cell Line; Chemical Fractionation; Chromatography, Agarose; Histones; Humans; Mice; Mitosis; Molecular Chaperones; Nucleosomes; Phosphorylation; Protein Processing, Post-Translational; Sepharose; Sodium Chloride; Urea | 2009 |
High-yield expression of human vascular endothelial growth factor VEGF(165) in Escherichia coli and purification for therapeutic applications.
Topics: Arginine; Chromatography, High Pressure Liquid; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Fermentation; Humans; Hydrogen-Ion Concentration; Inclusion Bodies; Protein Folding; Recombinant Proteins; Sepharose; Urea; Vascular Endothelial Growth Factor A | 2010 |
Ion-exchange chromatographic protein refolding.
Topics: Adsorption; Chromatography, Ion Exchange; Models, Chemical; Muramidase; Protein Refolding; Proteins; Sepharose; Surface Properties; Urea | 2010 |
Characterization of goat plasma vitronectin.
Topics: Animals; Cell Adhesion; Chlorocebus aethiops; Goats; Molecular Weight; Peptide Fragments; Protein Denaturation; Protein Multimerization; Protein Structure, Quaternary; Proteolysis; Sepharose; Urea; Vero Cells; Vitronectin | 2003 |
Salt-urea, sulfopropyl-sepharose, and covalent chromatography methods for histone isolation and fractionation.
Topics: Animals; Chemical Fractionation; Chromatography; HeLa Cells; Histones; Humans; Mice; Pressure; Sepharose; Sodium Chloride; Urea | 2014 |
Swellable Gelatin Methacryloyl Microneedles for Extraction of Interstitial Skin Fluid toward Minimally Invasive Monitoring of Urea.
Topics: Body Fluids; Cell Survival; Cross-Linking Reagents; Female; Gelatin; HaCaT Cells; Humans; Hydrogels; Methacrylates; Molecular Weight; Needles; Sepharose; Skin; Spectroscopy, Fourier Transform Infrared; Thermogravimetry; Urea; Water | 2020 |