urea has been researched along with dithiothreitol in 210 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 126 (60.00) | 18.7374 |
| 1990's | 37 (17.62) | 18.2507 |
| 2000's | 39 (18.57) | 29.6817 |
| 2010's | 8 (3.81) | 24.3611 |
| 2020's | 0 (0.00) | 2.80 |
| Authors | Studies |
|---|---|
| Adiga, PR; Murthy, US; Podder, SK | 1 |
| Phipps, RJ; Richardson, PS | 1 |
| Gardell, SJ; Tate, SS | 2 |
| Bjorn, MJ; Iglewski, BH; Pavlovskis, OR; Thompson, MR | 1 |
| Nakamura, T; Yamaguchi, M; Yanagisawa, T | 1 |
| Etchison, D; Walter, G | 1 |
| Golbeck, JH; Kok, B | 1 |
| Bjorn, MJ; Iglewski, BH; Maxwell, ES; Sadoff, J | 1 |
| Duncan, CL; Labbe, RG; Reich, RR | 1 |
| D'Agnolo, G; Rosenfeld, IS; Vagelos, PR | 1 |
| Arellano-Blanco, J; Becerril-Meza, G; Fernández-Quintero, P; Gómez-Estrada, H; López-de la Rosa, LM | 1 |
| Beato, M; Puigdoménech, P | 1 |
| Hicks, RM; Warren, RC | 1 |
| Finlay, TH; Johnson, AJ; Marcus, DL | 1 |
| Irie, M; Yano, Y | 1 |
| Waites, WM; Wyatt, LR | 2 |
| Boesman, M; Heusser, CH; Isliker, H; Jacot-Guillarmod, H; Knobel, HR | 1 |
| Sleytr, UB; Thorne, KJ | 1 |
| Kay, E | 1 |
| Khan, MA; Litt, M; Wolf, DP | 1 |
| Mazia, D; Schatten, G; Steinhardt, R | 1 |
| Matsuda, M; Yoneda, M | 1 |
| Adelman, TG; Arosio, P; Drysdale, JW | 1 |
| Chen, AB; Huseby, RM; Mosesson, MW | 1 |
| Shapiro, BM | 1 |
| Heyde, E; Morrison, JF | 1 |
| Chan, E; Desforges, JF | 1 |
| Fawcett, DW; Hamilton, DW; Olson, GE | 1 |
| Fountoulakis, M | 1 |
| Olesen, PR; Ortwerth, BJ | 1 |
| al-Zuhdi, Y; Bottazzo, GF; Foxon, R; Gillott, DJ; Gray, A; Jassim, A | 1 |
| Arthur, M; Bloomfield, SF | 1 |
| FitzGerald, D; Ogata, M; Pastan, I | 1 |
| Birmes, A; Maurer, KH; Riesner, D; Sättler, A | 1 |
| Kim, BW; Lee, HS; Majima, Y; Sakakura, Y | 1 |
| Fesus, L; Harsfalvi, J; Laczko, J; Loewy, AG; Tarcsa, E | 1 |
| Hiraoka, Y; Ikeguchi, M; Kuwajima, K; Sugai, S; Sugawara, T | 1 |
| Hachimori, A; Koyama, T; Uchida, Y | 1 |
| de Jong, MD; van Hoek, AN; van Os, CH | 1 |
| Peberdy, JF; Vainstein, MH | 1 |
| Buchanan, MJ; Imam, SH; Shin, HC; Snell, WJ | 1 |
| Pizzo, SV; Roche, PA; Salvesen, GS | 1 |
| Brown, ML; Feuer, DS; Hesler, CB; Marcel, YL; Milne, RW; Tall, AR | 1 |
| Lei, DL; Matsuda, M; Okabe, T; Ozutsumi, K; Sugimoto, N | 1 |
| Dogterom, P; Mulder, GJ; Nagelkerke, JF | 1 |
| Altamirano, MM; Calcagno, M; Mulliert, G | 1 |
| Demarquoy, J; Gautier, C; Vaillant, R | 1 |
| Boni-Schnetzler, M; O'Hare, T; Pilch, PF; Rubin, J | 1 |
| Deutsch, HF; Han, KK; Xu, LX; Zhang, A; Zhu, DX; Zhu, NC | 1 |
| Charlemagne, D; Geering, K; Mayoux, E; Oliviero, P; Poyard, M | 1 |
| Bray, BA | 1 |
| Blobel, G; Chirico, WJ; Waters, MG | 1 |
| DeCaprio, AP; Jackowski, SJ; Regan, KA | 1 |
| Powers, DB; Wells, JA | 1 |
| Snyder, GH | 1 |
| Kume, K; Nakai, T; Samejima, Y; Sugimoto, C | 1 |
| Goodearl, AD; Moreton, K; Williams, J | 1 |
| Grunfeld, C; Ramachandran, J; Shigenaga, JK | 1 |
| Gibson, D; Levanon, M; Smithies, O | 1 |
| Snell, EE; Wada, H | 1 |
| Ibrahim, AB; Rao, KR; Vyas, GN | 1 |
| Dreesman, GR; Hollinger, FB; McCombs, RM; Melnick, JL | 1 |
| Delespesse, G; Ishizaka, K; King, TP; Kishimoto, T | 1 |
| Vary, JC | 1 |
| Beale, D | 1 |
| O'Sullivan, WJ | 1 |
| Haino-Fukushima, K | 1 |
| Halbert, SP; Lin, TM; Paskevitz, A | 1 |
| Bilello, P; Drzeniek, R | 1 |
| Brandts, JF; Gelb, WG; Nordin, JH | 1 |
| Duncan, CL; Frieben, WR | 1 |
| Billups, C; Kay, E; Strickland, EH | 1 |
| Chipman, DM; Garvin, JH | 1 |
| Ishizu, K; Kimura, T; Sugiura, Y | 1 |
| Scott, WA | 1 |
| Richmond, V | 1 |
| Garewal, HS; Wasserman, AR | 1 |
| Kruiswijk, T; Planta, RJ | 1 |
| Nelson, TE; Watts, TE | 1 |
| Barsano, C; Degroot, LJ; Refetoff, S; Strausser, J | 1 |
| Holmgren, A | 1 |
| Donovan, E; Friedkin, M | 1 |
| Ristow, SS; Wetlaufer, DB | 1 |
| Lapanje, S; Rupley, JA | 1 |
| Ishihama, A; Ito, K | 1 |
| Minatogawa, S; Vold, B | 1 |
| Björk, I; Hammarström, S; Westöö, A | 1 |
| Chung, AE | 1 |
| Koerner, D; Oppenheimer, JH; Surks, MI | 1 |
| Ness, AT; Pastewka, JV; Peacock, AC; Reed, RA | 1 |
| Ando, Y; Steiner, M | 1 |
| Iyer, KS; Klee, WA | 1 |
| Capaldi, RA; Hunter, DR; Komai, H | 1 |
| Gunsalus, IC; Yu, C | 1 |
| Davies, DA; Hess, M | 1 |
| Bergdoll, MS; Catsimpoolas, N; Schantz, EJ; Yotis, WW | 1 |
| Jörnvall, H; Prozorovski, V | 1 |
| Bhattacharyya, SN; Lynn, WS; Passero, MA | 1 |
| Hörmann, H; Stemberger, A | 1 |
| Axén, R; Brocklehurst, K; Carlsson, J; Crook, EM | 1 |
| Metzger, JF; Spero, L; Warren, JR | 1 |
| Callahan, HJ; Liberti, PA; Maurer, PH | 1 |
| Edelman, GM; Millette, CF | 1 |
| Taylor, RT | 1 |
| Hamilton, MG; Pavlovec, A; Petermann, ML | 1 |
| Aronson, AI; Fitz-James, PC | 1 |
| Kawasaki, H; Sato, H; Suzuki, M | 1 |
| Grey, HM; Zimmerman, B | 1 |
| Burka, ER | 1 |
| Gross, PA; Liebhaber, H | 1 |
| Otsuki, E; Shimadzu, T; Tashiro, Y | 1 |
| Fukui, S; Isemura, Y; Kondo, M; Toraya, T | 1 |
| Demorest, DM; Stahmann, MA | 1 |
| Cotlier, E; Klein, J | 1 |
| McIntosh, ME; Watt, DD | 1 |
| Buchwald, BM | 1 |
| Bloemendal, H; Hoenders, HJ; van Kamp, GJ | 1 |
| Griggs, LJ; Holden, KG; Weisbach, JA; Yim, NC | 1 |
| Magnuson, JA; Magnuson, NS | 1 |
| Bloemendal, H; Gerding, JJ; Hagel, P; Koppers, A | 1 |
| Stanton, M; Viswanatha, T | 1 |
| Garewal, HS; Singh, J; Wasserman, AR | 1 |
| Hastings, JW; Meighen, EA; Nicoli, MZ | 1 |
| Catsimpoolas, N; Wang, J | 1 |
| Abraham, S; Smith, S | 1 |
| Chen, JC; Fisher, RJ; Kaplay, SS; Sanadi, DR; Sani, BP | 1 |
| Bloemendal, H; Matze, R; Schoenmakers, JG; Van Poppel, M | 1 |
| Hermans, J; Robinson, RM | 1 |
| Müller, HW | 1 |
| Klose, J; Zeindl, E | 1 |
| Litt, M; Marriott, C; Martin, R | 1 |
| McClard, RW | 1 |
| Sim, E; Sim, RB | 1 |
| Grossman, SH; Pyle, J; Steiner, RJ | 1 |
| Sobhon, P; Tanphaichitr, N; Thungkasemvathana, P | 1 |
| Folk, JE; Seelig, GF | 1 |
| Gazarian, IG; Tien, M; Whitwam, RE | 1 |
| Braswell, EH; Green, BN; Kuchumov, A; Malte, H; Oliver, RW; Sharma, PK; Vinogradov, SN; Weber, RE | 1 |
| Ramasarma, PR; Rao, AG; Rao, DR | 1 |
| Freedman, RB; Hawkins, HC | 1 |
| McClelland, DA; Price, NC; Stevens, L | 1 |
| Gautier, MF; Gavalda, S; Joudrier, P; Lullien-Pellerin, V | 1 |
| Hirose, M; Takahashi, N; Tatsumi, E | 1 |
| Chen, YL; Jassim, A | 1 |
| Fujii, T; Hayashi, T; Sakai, K | 1 |
| Chirgwin, JM; Horowitz, PM; Miller-Martini, DM | 1 |
| Berthold, H; Northemann, W; Steffens, U | 1 |
| Goodman, N; King, AD; Smith, PT | 1 |
| Haas, MJ; Joerger, RD | 1 |
| Creighton, TE; Ewbank, JJ | 1 |
| Daniel, RM; Petach, HH; Piller, K | 1 |
| El'Skaya, A; Gorchkov, D; Jaffrezic-Renault, N; Jdanova, A; Martelet, C; Poyard, S; Soldatkin, A | 1 |
| Arunachalam, U; Kellis, JT | 1 |
| Lightowlers, MW; Rothel, JS; Seow, HF; Wood, PR | 1 |
| Seiffert, D; Wagner, NV | 1 |
| Alvergna, P; Brigotti, M; Carnicelli, D; Mazzaracchio, R; Montanaro, L; Sperti, S | 1 |
| Lilja, H; Lundwall, A; Malm, J; Peter, A | 1 |
| Apostol, I; Lippincott, J | 1 |
| Falconer, RJ; Middelberg, AP; O'Neill, BK | 2 |
| Tang, B; Wei, C; Yang, K; Zhang, Y | 1 |
| Martínez-Chantar, ML; Pajares, MA | 1 |
| Campoy, FJ; Fini, C; Flores-Flores, C; Martínez-Martínez, A; Muñoz-Delgado, E; Rodríguez-López, JN; Vidal, CJ | 1 |
| Blank, VC; Peña, C; Roguin, LP | 1 |
| Huang, K; Park, YD; Zhou, HM | 1 |
| Patston, PA; Simonovic, I | 1 |
| Cigić, B; Dahl, SW; Pain, RH | 1 |
| Clarke, AE; Poon, S; Schultz, CJ | 1 |
| Dagonet, F; Ferrari, P; Labat-Robert, J; Menasche, M | 1 |
| Chaudhuri, AR; Khan, IA; Ludueña, RF | 1 |
| Johnson, JL; Lasagna, MD; Reinhart, GD | 1 |
| Vaccaro, E; Waite, JH | 1 |
| Minneman, KP; Robeva, A; Rogge, G; Uberti, M; Vicentic, A | 1 |
| Liu, XW; Sok, DE | 1 |
| Banci, L; Bartalesi, I; Ciofi-Baffoni, S; Tien, M | 1 |
| Castro, HC; Foguel, D; Monteiro, RQ; Zingali, RB | 1 |
| Ito, M; Kato, Y; Nagata, K; Oda, Y; Suzuki, K; Tanokura, M; Yamagoe, S | 1 |
| Pfeiffer, SE; Taylor, CM | 1 |
| Altland, K; Winter, P | 1 |
| Gellon, JO; Girault, HH; Jensen, H; Lion, N | 1 |
| Batista-Viera, F; Betancor, L; Cuadra, K; Grazú, V; Manta, C; Ovsejevi, K | 1 |
| Alloza, I; Hawthorne, S; Martens, E; Vandenbroeck, K | 1 |
| Meng, FG; Wang, XY; Zhou, HM | 1 |
| Gragen, S; Li, Y; Liu, J; Ma, Y; Matoney, L; Song, X; Yan, B; Yang, D | 1 |
| Cho, S; Kho, CW; Lee, DH; Myung, PK; Park, BC; Park, SG | 1 |
| Choi, NS; Hahm, JH; Kim, SH; Maeng, PJ | 1 |
| Song, GY; Wang, M; Wang, XY | 1 |
| Begum, S; Encheva, V; Gharbia, SE; Shah, HN; Wait, R | 1 |
| Chang, JY; Lin, CC; Lu, BY | 1 |
| Hong, Y; Li, Z; Liu, B; Ni, J; Shen, Y; Sheng, D | 1 |
| Franco, RS; Jiang, M; Joiner, CH; Rettig, RK; Risinger, M | 1 |
| Schröder, HC; Wilkesman, JG | 1 |
| Hsieh, HJ; Lin, JL; Ruaan, RC | 1 |
| Markovich, D; Nicke, A; Regeer, RR | 1 |
| Ahmad, MF; Ramakrishna, T; Raman, B; Rao, ChM | 1 |
| Fu, XT; Kim, SM; Lin, H | 1 |
| Gallagher, SR | 1 |
| Kim, KB; Ko, YG; Lee, JS | 1 |
| Wingfield, PT | 1 |
| Chen, HM; Chiang, CY; Kao, SH; Wong, HK | 1 |
| An, M; An, Y; Ji, J; Pu, H; Ren, X; Shan, Y; Wang, Q; Wei, S; Wu, J | 1 |
| Ito, T; Kato, A; Nakajima, T; Sugiyama, G; Tsukue, N | 1 |
| Liu, KN; Wang, BW; Wang, SS | 1 |
| Clerens, S; Cornellison, CD; Deb-Choudhury, S; Dyer, JM; Grosvenor, AJ; Plowman, JE; Thomas, A | 1 |
| Almeida, AM; Cameán, A; Campos, A; Coelho, AV; Prieto, A; Puerto, M; Vasconcelos, V | 1 |
| Barbarito, G; Bartolucci, M; Calzia, D; Panfoli, I; Ravera, S | 1 |
| Ki, CS; Lin, CC; Lin, TY | 1 |
| Delcour, JA; Lambrecht, MA; Rombouts, I; Van Kelst, L | 1 |
2 review(s) available for urea and dithiothreitol
| Article | Year |
|---|---|
The anatomy, physiology, pharmacology and pathology of tracheobronchial mucus secretion and the use of expectorant drugs in human disease.
Topics: Animals; Arginine; Ascorbic Acid; Bronchi; Carbonates; Copper; Detergents; Dithiothreitol; Expectorants; Glucocorticoids; Humans; Hydrolases; Iodides; Ipecac; Mucus; Quaternary Ammonium Compounds; Respiratory Tract Diseases; Trachea; Urea; Water | 1978 |
The isolation of detergent-resistant lipid rafts for two-dimensional electrophoresis.
Topics: Animals; Detergents; Dithiothreitol; Electrophoresis, Gel, Two-Dimensional; Humans; Membrane Microdomains; Membrane Proteins; Mice; Thiourea; Urea | 2008 |
208 other study(ies) available for urea and dithiothreitol
| Article | Year |
|---|---|
The interaction of riboflavin with a protein isolated from hen's egg white: a spectrofluorimetric study.
Topics: Binding Sites; Bromosuccinimide; Dithiothreitol; Egg Proteins; Egg White; Hydrogen-Ion Concentration; Kinetics; Mathematics; Oxidation-Reduction; Protein Binding; Protein Conformation; Riboflavin; Spectrometry, Fluorescence; Urea | 1976 |
Latent proteinase activity of gamma-glutamyl transpeptidase light subunit.
Topics: Animals; Disulfides; Dithiothreitol; Endopeptidases; Enzyme Activation; gamma-Glutamyltransferase; Kidney; Kinetics; Macromolecular Substances; Rats; Urea | 1979 |
Production of exoenzyme S during Pseudomonas aeruginosa infections of burned mice.
Topics: Animals; Bacterial Toxins; Burns; Dithiothreitol; Female; Mice; Nucleoside Diphosphate Sugars; Nucleotidyltransferases; Pentosyltransferases; Pseudomonas aeruginosa; Pseudomonas Infections; Ribose; Urea | 1979 |
Studies on the subunits of myosin from muscle layer of Ascaris lumbricoides suum.
Topics: Adenosine Triphosphatases; Ascaris; Chemical Precipitation; Dithionitrobenzoic Acid; Dithiothreitol; Molecular Weight; Muscles; Myosins; Peptide Fragments; Urea | 1975 |
Subunit interactions in polyoma virus structure.
Topics: Capsid; Cell Line; Centrifugation, Density Gradient; Dithiothreitol; DNA, Viral; Electrophoresis, Polyacrylamide Gel; Hemagglutination; Histones; Maleimides; Polyomavirus; Sodium Dodecyl Sulfate; Urea; Viral Proteins | 1977 |
Further studies of the membrane--bound iron--sulfur proteins and P700 in a photosystem I subchloroplast particle.
Topics: Chloroplasts; Dithiothreitol; Electron Spin Resonance Spectroscopy; Ferric Compounds; Ferricyanides; Iron-Sulfur Proteins; Membrane Proteins; Metalloproteins; Oxidation-Reduction; Photochemistry; Protein Denaturation; Spectrum Analysis; Urea | 1978 |
Pseudomonas aeruginosa exoenzyme S: an adenosine diphosphate ribosyltransferase distinct from toxin A.
Topics: Adenosine Diphosphate Sugars; Bacterial Toxins; Diphtheria Toxin; Dithiothreitol; Hot Temperature; Molecular Weight; NAD; Pentosyltransferases; Protein Denaturation; Proteins; Pseudomonas aeruginosa; Ribose; Substrate Specificity; Urea | 1978 |
Alteration in ultrastructure and germination of Clostridium perfringens type A spores following extraction of spore coats.
Topics: Clostridium; Clostridium perfringens; Dithiothreitol; Hot Temperature; Mercaptoethanol; Sodium Hydroxide; Spores, Bacterial; Urea | 1978 |
Beta-Ketoacyl-acyl carrier protein synthetase. Characterization of the acyl-enzyme intermediate.
Topics: 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase; Acetyl Coenzyme A; Acyltransferases; Alanine; Caproates; Caprylates; Carbon Radioisotopes; Chromatography, Gel; Chromatography, Thin Layer; Circular Dichroism; Coenzyme A; Dithiothreitol; Escherichia coli; Fatty Acids; Hydrogen-Ion Concentration; Hydroxamic Acids; Malonates; Mercaptoethanol; Tritium; Urea | 1975 |
[Immunogenicity of L5178Y cells modified by different reagents].
Topics: Animals; Antibody Formation; Dithiothreitol; Glutaral; Immunity, Cellular; Iodoacetamide; Lymphoma; Mercaptoethanol; Mice; Mice, Inbred BALB C; Neoplasms, Experimental; Neuraminidase; Potassium Iodide; Rats; Urea | 1977 |
Nuclear magnetic resonance studies on rabbit uteroglobin.
Topics: Animals; Dithiothreitol; Female; Glycoproteins; Magnetic Resonance Spectroscopy; Protein Conformation; Rabbits; Urea; Uteroglobin | 1977 |
Chemical dissection and negative staining of the bladder luminal membrane.
Topics: Cell Membrane; Detergents; Dithiothreitol; Glycoside Hydrolases; Guanidines; Peptide Hydrolases; Phospholipases; Statistics as Topic; Urea; Urinary Bladder | 1978 |
Purification and properties of porcine platelet aggregating factor.
Topics: Amino Acids; Animals; Blood Coagulation Factors; Carbohydrates; Cysteine; Dithiothreitol; Factor VIII; Guanidines; Lysine; Platelet Aggregation; Swine; Urea | 1978 |
Renaturation of yeast inorganic pyrophosphatase denatured in urea and guanidine hydrochloride.
Topics: Bromosuccinimide; Dithiothreitol; Glutathione; Guanidines; Macromolecular Substances; Mercaptoethanol; Molecular Weight; Osmolar Concentration; Protein Denaturation; Pyrophosphatases; Saccharomyces cerevisiae; Sulfhydryl Compounds; Tryptophan; Urea | 1975 |
The effect of chlorine on spores of Clostridium bifermentans, Bacillus subtilis and Bacillus cereus.
Topics: Bacillus cereus; Bacillus subtilis; Bacterial Proteins; Clostridium; Dithiothreitol; Hot Temperature; Muramidase; Sodium Hypochlorite; Spores, Bacterial; Urea | 1975 |
The effect of reduction and reoxidation on the biological activities and structure of human Clq (first component of complement).
Topics: Binding Sites; Complement C1; Complement System Proteins; Disulfides; Dithiothreitol; Hemolysis; Humans; Immunodiffusion; Immunoelectrophoresis; Oxidation-Reduction; Protein Binding; Protein Conformation; Urea | 1975 |
Chemical characterization of the regularly arranged surface layers of Clostridium thermosaccharolyticum and Clostridium thermohydrosulfuricum.
Topics: Amino Acids; Bacterial Proteins; Cell Wall; Clostridium; Dithiothreitol; Edetic Acid; Galactose; Glucose; Glycoproteins; Guanidines; Mannose; Molecular Weight; Polyethylene Glycols; Potassium Chloride; Rhamnose; Urea | 1976 |
Origins of circular dichroism bands in Bowman-Birk soybean trypsin inhibitor.
Topics: Binding Sites; Circular Dichroism; Disulfides; Dithiothreitol; Guanidines; Imidazoles; Protein Binding; Protein Conformation; Spectrophotometry, Ultraviolet; Trypsin Inhibitor, Bowman-Birk Soybean; Trypsin Inhibitors; Urea | 1976 |
Effect of mucolytic agents on the rheological properties of tracheal mucus.
Topics: Animals; Dithiothreitol; Dogs; Expectorants; Guanidines; Male; Mucus; Rheology; Solubility; Thiocyanates; Trachea; Urea; Viscosity | 1976 |
Turning on of activities in unfertilized sea urchin eggs: correlation with changes of the surface.
Topics: Ammonia; Animals; Cell Membrane; Dithiothreitol; Female; Fertilization; Glycerol; Membrane Potentials; Ovum; Sea Urchins; Seawater; Surface Properties; Urea | 1975 |
Isolation and purification of two antigenically active, "complimentary" polypeptide fragments of tetanus neurotoxin.
Topics: Antigens, Bacterial; Chromatography, Gel; Clostridium tetani; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Flocculation Tests; Hot Temperature; Peptides; Tetanus Toxin; Urea | 1975 |
Multiple subunits in human ferritins: evidence for hybrid molecules.
Topics: Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Ferritins; Humans; Isoelectric Focusing; Liver; Macromolecular Substances; Protein Binding; Sodium Dodecyl Sulfate; Urea | 1975 |
The cold-insoluble globulin of human plasma: studies of its essential structural features.
Topics: Amino Acids; Circular Dichroism; Cryoglobulins; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Hexoses; Humans; Macromolecular Substances; Molecular Weight; Protein Conformation; Solubility; Ultracentrifugation; Urea | 1975 |
Limited proteolysis of some egg surface components is an early event following fertilization of the sea urchin, Strongylocentrotus purpuratus.
Topics: Animals; Dithiothreitol; Female; Fertilization; Ovum; Parthenogenesis; Peptide Hydrolases; Proteins; Sea Urchins; Urea | 1975 |
Studies on inosine monophosphate dehydrogenase. An associating-dissociating system.
Topics: Amino Acids; Dithiothreitol; Enterobacter; IMP Dehydrogenase; Ketone Oxidoreductases; Kinetics; Macromolecular Substances; Mercaptoethanol; Molecular Weight; Protein Conformation; Sodium Dodecyl Sulfate; Urea | 1976 |
The role of disulphide bonds in Heinz body attachment to membranes.
Topics: Binding Sites; Cell Fractionation; Cell Membrane; Disulfides; Dithiothreitol; Erythrocytes; Heinz Bodies; Heme; Humans; Mercaptoethanol; Urea | 1976 |
Isolation and characterization of the fibrous sheath of rat epididymal spermatozoa.
Topics: Animals; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Epididymis; Flagella; Male; Peptides; Polyethylene Glycols; Potassium; Rats; Sodium Dodecyl Sulfate; Solubility; Spermatozoa; Thiocyanates; Urea | 1976 |
Unfolding intermediates of the extracellular domain of the interferon gamma receptor.
Topics: Blotting, Western; Catalysis; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Guanidine; Guanidines; Humans; Interferons; NADP; Oxidation-Reduction; Protein Conformation; Receptors, Immunologic; Receptors, Interferon; Thioredoxin-Disulfide Reductase; Thioredoxins; Urea | 1992 |
Studies on the solubilization of the water-insoluble fraction from human lens and cataract.
Topics: Amino Acids; Cataract; Chromatography, Gel; Crystallins; Dithiothreitol; Humans; Lens, Crystalline; Solubility; Sonication; Urea | 1992 |
Isolation and biochemical characterization of the human sperm tail fibrous sheath.
Topics: Antibodies, Monoclonal; Cytoskeletal Proteins; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Fluorescent Antibody Technique; Humans; Male; Polyethylene Glycols; Sperm Tail; Spermatozoa; Urea | 1992 |
Interaction of Bacillus subtilis spores with sodium hypochlorite, sodium dichloroisocyanurate and chloramine-T.
Topics: Bacillus subtilis; Chloramines; Disinfectants; Dithiothreitol; Muramidase; Peptidoglycan; Picolinic Acids; Sodium Dodecyl Sulfate; Sodium Hydroxide; Sodium Hypochlorite; Spores, Bacterial; Tosyl Compounds; Triazines; Urea | 1992 |
Analysis of Pseudomonas exotoxin activation and conformational changes by using monoclonal antibodies as probes.
Topics: Adenosine Diphosphate Ribose; ADP Ribose Transferases; Animals; Antibodies, Monoclonal; Bacterial Toxins; Cell Line; Dithiothreitol; Enzyme-Linked Immunosorbent Assay; Epitopes; Exotoxins; Female; Hydrogen-Ion Concentration; Mice; Mice, Inbred BALB C; Protein Conformation; Pseudomonas aeruginosa; Pseudomonas aeruginosa Exotoxin A; Urea; Virulence Factors | 1991 |
Analysis of the conformational transitions of proteins by temperature-gradient gel electrophoresis.
Topics: alpha-Amylases; Aspergillus oryzae; Bacillus subtilis; Buffers; Calcium; Dithiothreitol; Electrophoresis; Hydrogen-Ion Concentration; Protein Conformation; Silver; Staining and Labeling; Surface-Active Agents; Temperature; Urea | 1990 |
A technique for quantitative cytology of nasal secretions.
Topics: Cell Count; Chronic Disease; Cytological Techniques; Dithiothreitol; Edetic Acid; Humans; Mucus; Nasal Mucosa; Peptide Hydrolases; Propylene Glycol; Propylene Glycols; Sinusitis; Urea | 1991 |
The presence of a covalently cross-linked matrix in human platelets.
Topics: Amino Acids; Blood Platelets; Blood Proteins; Dipeptides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Humans; Immunoenzyme Techniques; Macromolecular Substances; Molecular Weight; Thrombin; Urea | 1991 |
Kinetics of disulfide bond reduction in alpha-lactalbumin by dithiothreitol and molecular basis of superreactivity of the Cys6-Cys120 disulfide bond.
Topics: Chemical Phenomena; Chemistry, Physical; Circular Dichroism; Cysteine; Cystine; Dithiothreitol; Edetic Acid; Kinetics; Lactalbumin; Oxidation-Reduction; Protein Conformation; Protein Denaturation; Spectrophotometry, Ultraviolet; Urea | 1990 |
Stability and conformation of porcine phosphofructokinase M and L.
Topics: Animals; Circular Dichroism; Dithiothreitol; Edetic Acid; Isoenzymes; Kinetics; Liver; Myocardium; Phosphofructokinase-1; Protein Conformation; Swine; Urea | 1990 |
Effects of dimethylsulfoxide and mercurial sulfhydryl reagents on water and solute permeability of rat kidney brush border membranes.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Cell Membrane Permeability; Cysteine; Dimethyl Sulfoxide; Dithiothreitol; Kidney; Kinetics; Mannitol; Mercury; Microvilli; Osmosis; Rats; Sodium Chloride; Sulfhydryl Reagents; Urea; Water | 1990 |
Solubilisation of a cell wall bound invertase in Aspergillus nidulans.
Topics: Aspergillus nidulans; beta-Fructofuranosidase; Cell Wall; Chymotrypsin; Dithiothreitol; Endopeptidase K; Ethanol; Glycoside Hydrolases; Octoxynol; Polyethylene Glycols; Polysorbates; Serine Endopeptidases; Sodium Dodecyl Sulfate; Urea | 1990 |
The Chlamydomonas cell wall: characterization of the wall framework.
Topics: Carbohydrates; Cell Wall; Chlamydomonas; Dithiothreitol; Extracellular Matrix; Mucoproteins; Peptides; Sarcosine; Sodium Dodecyl Sulfate; Stress, Mechanical; Urea | 1985 |
Symmetry of the inhibitory unit of human alpha 2-macroglobulin.
Topics: alpha-Macroglobulins; Dithiothreitol; Fibrinolysin; Humans; In Vitro Techniques; Molecular Weight; Protein Conformation; Trypsin Inhibitors; Urea | 1988 |
Structure-function analysis of plasma cholesteryl ester transfer protein by protease digestion and expression of cDNA fragments in Escherichia coli.
Topics: Antibodies, Monoclonal; Blotting, Western; Carrier Proteins; Cholesterol Ester Transfer Proteins; Chromatography, Gel; Chymotrypsin; Cloning, Molecular; Dithiothreitol; DNA; Electrophoresis, Polyacrylamide Gel; Epitopes; Escherichia coli; Glycoproteins; Humans; Macromolecular Substances; Molecular Weight; Peptide Fragments; Peptide Hydrolases; Protein Conformation; Serine Endopeptidases; Structure-Activity Relationship; Trypsin; Urea | 1989 |
Isolation, purification, and characterization of fragment B, the NH2-terminal half of the heavy chain of tetanus toxin.
Topics: Amino Acid Sequence; Animals; Chromatography, High Pressure Liquid; Dithiothreitol; Epitopes; Immunodiffusion; Ion Channels; Isoelectric Point; Lipid Bilayers; Mice; Molecular Sequence Data; Molecular Weight; Peptide Fragments; Tetanus Toxin; Urea | 1989 |
Lipid peroxidation-dependent and -independent protein thiol modifications in isolated rat hepatocytes: differential effects of vitamin E and disulfiram.
Topics: 1-Propanol; Animals; Bromisovalum; Cell Survival; Cells, Cultured; Disulfiram; Dithiothreitol; Glutathione; Glutathione Disulfide; Lipid Peroxidation; Liver; Male; Maleates; Propanols; Proteins; Rats; Sulfhydryl Compounds; Urea; Vitamin E | 1989 |
Sulfhydryl groups of glucosamine-6-phosphate isomerase deaminase from Escherichia coli.
Topics: Allosteric Regulation; Chemical Phenomena; Chemistry; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Activation; Enzyme Reactivators; Escherichia coli; Glucose-6-Phosphate Isomerase; Hydrocarbons, Iodinated; Kinetics; Periodic Acid; Protein Denaturation; Structure-Activity Relationship; Sulfhydryl Compounds; Urea | 1987 |
[Effects of dithiothreitol and thioredoxines on the in vitro activity of enzymes of the urea cycle in rats].
Topics: Animals; Bacterial Proteins; Dithiothreitol; Enzyme Activation; Immunologic Techniques; Liver; Magnesium; Male; Rats; Rats, Inbred Strains; Thioredoxins; Urea | 1986 |
The ligand binding subunit of the insulin-like growth factor 1 receptor has properties of a peripheral membrane protein.
Topics: Dithiothreitol; Female; Humans; Insulin-Like Growth Factor I; Macromolecular Substances; Membrane Proteins; Microsomes; Molecular Weight; Placenta; Pregnancy; Receptors, Cell Surface; Receptors, Somatomedin; Urea | 1986 |
Investigations of primary and secondary structure of porcine ubiquitin. Its N epsilon-acetylated lysine derivative.
Topics: Acetylation; Amino Acid Sequence; Animals; Cattle; Circular Dichroism; Dithiothreitol; Erythrocytes; Guanidine; Guanidines; High Mobility Group Proteins; Lysine; Male; Protein Conformation; Swine; Ubiquitins; Urea | 1986 |
Identification of two isoforms of the catalytic subunit of Na,K-ATPase in myocytes from adult rat heart.
Topics: Alkylation; Animals; Brain; Dithiothreitol; Iodoacetamide; Isoenzymes; Kidney; Macromolecular Substances; Myocardium; Rats; Sarcolemma; Sodium-Potassium-Exchanging ATPase; Urea | 1987 |
Quantification of tissue fibronectin from terminal villi of placenta.
Topics: Chorionic Villi; Dithiothreitol; Enzyme-Linked Immunosorbent Assay; Female; Fibrinolysin; Fibronectins; Heparin; Humans; Methods; Pregnancy; Solubility; Urea | 1985 |
70K heat shock related proteins stimulate protein translocation into microsomes.
Topics: Adenosine Triphosphate; Affinity Labels; Biological Transport, Active; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Heat-Shock Proteins; Intracellular Membranes; Microsomes; Photochemistry; Protein Denaturation; Proteins; Saccharomyces cerevisiae; Urea | 1988 |
Mechanism of formation and quantitation of imines, pyrroles, and stable nonpyrrole adducts in 2,5-hexanedione-treated protein.
Topics: Amino Acids; Chemical Phenomena; Chemistry; Dithiothreitol; Hexanones; Imines; Ketones; Peptide Hydrolases; Proteins; Pyrroles; Sodium Dodecyl Sulfate; Urea | 1987 |
In vivo formation and stability of engineered disulfide bonds in subtilisin.
Topics: Autolysis; Bacillus; Computers; Cysteine; Disulfides; Dithiothreitol; Half-Life; Models, Molecular; Mutation; Subtilisins; Temperature; Urea | 1986 |
Intramolecular disulfide loop formation in a peptide containing two cysteines.
Topics: Cyanogen Bromide; Cysteine; Disulfides; Dithiothreitol; Glutathione; Guanidine; Guanidines; Iodoacetates; Kinetics; Oxidation-Reduction; Peptide Fragments; Protein Conformation; Trypsin Inhibitor, Kunitz Soybean; Trypsin Inhibitors; Urea | 1987 |
Properties of dermonecrotic toxin prepared from sonic extracts Bordetella bronchiseptica.
Topics: Animals; Bacterial Toxins; Biological Assay; Bordetella; Dithiothreitol; Guinea Pigs; Hot Temperature; Macromolecular Substances; Molecular Weight; Sonication; Urea | 1986 |
Selective reduction of a disulphide bridge in hen ovotransferrin.
Topics: Alkylation; Animals; Chickens; Conalbumin; Cysteine; Disulfides; Dithiothreitol; Egg Proteins; Female; Iron; Oxidation-Reduction; Peptide Fragments; Protein Conformation; Protein Denaturation; Urea | 1985 |
Urea treatment allows dithiothreitol to release the binding subunit of the insulin receptor from the cell membrane: implications for the structural organization of the insulin receptor.
Topics: Cell Line; Cell Membrane; Chemical Phenomena; Chemistry; Dithiothreitol; Humans; Lymphocytes; Protein Denaturation; Receptor, Insulin; Urea | 1985 |
Heterogeneity of normal human immunoglobulin light chains. Nonallelic variation in the constant region of lambda chains.
Topics: Acetamides; Amino Acids; Autoanalysis; Bence Jones Protein; Chemical Phenomena; Chemistry; Chromatography, DEAE-Cellulose; Chromatography, Gel; Chromatography, Ion Exchange; Cysteamine; Disulfides; Dithiothreitol; Fluoroacetates; gamma-Globulins; Glycine; Humans; Hydrazines; Immunoglobulin G; Immunoglobulins; Iodoacetates; Methods; Peptides; Polymorphism, Genetic; Protein Denaturation; Serine; Sulfides; Trypsin; Urea | 1971 |
Isolation of large peptides by flat-bed polyacrylamide gel electrophoresis.
Topics: Acrylamides; Alkylation; Animals; Cattle; Cyanogen Bromide; Dithiothreitol; Egg White; Electrophoresis; Escherichia coli; Gels; Hydro-Lyases; Iodoacetates; Methods; Muramidase; Peptides; Serum Albumin, Bovine; Staining and Labeling; Tryptophan; Urea | 1972 |
Australia antigen (hepatitis B antigen): a conformational antigen dependent on disulfide bonds.
Topics: Acetates; Alkylation; Animals; Antibody Formation; Antigen-Antibody Reactions; Centrifugation, Density Gradient; Cross Reactions; Disulfides; Dithiothreitol; Epitopes; Guinea Pigs; Hemagglutination Inhibition Tests; Hepatitis B Antigens; Hypersensitivity, Delayed; Immunization; Immunoelectrophoresis; Iodoacetates; Mercaptoethanol; Oxidation-Reduction; Protein Conformation; Urea | 1972 |
Alteration of hepatitis B antigen (HB Ag) determinants by reduction and alkylation.
Topics: Acetamides; Alkylation; Animals; Antibody Formation; Centrifugation, Density Gradient; Dithiothreitol; Epitopes; Guinea Pigs; Hepatitis B Antigens; Immunodiffusion; Immunoelectrophoresis; Iodine; Iodine Radioisotopes; Microscopy, Electron; Oxidation-Reduction; Peptides; Phosphotungstic Acid; Radioimmunoassay; Serotyping; Staining and Labeling; Urea; Viral Proteins; Viral Vaccines | 1973 |
Immunogenic properties of modified antigen E. I. Presence of specific determinants for T cells in denatured antigen and polypeptide chains.
Topics: Alkylation; Allergens; Antibody Formation; Ascaris; Cells, Cultured; Chromatography, DEAE-Cellulose; Chromatography, Gel; Dinitrophenols; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Epitopes; Hemocyanins; Humans; Immune Sera; Immunoglobulin Fragments; Iodine Radioisotopes; Lymphocyte Activation; Oxidation-Reduction; Peptides; Pollen; Protein Denaturation; Radioimmunoassay; Sodium Dodecyl Sulfate; T-Lymphocytes; Tissue Extracts; Urea | 1974 |
Germination of Bacillus megaterium spores after various extraction procedures.
Topics: Bacillus megaterium; Bacterial Proteins; Colorimetry; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Hot Temperature; Picolinic Acids; Salicylates; Sodium Dodecyl Sulfate; Spores, Bacterial; Urea; Vibration | 1973 |
Fragmentation and reduction of porcine 19 S immunoglobulin M.
Topics: Animals; Densitometry; Dithiothreitol; Electrophoresis, Disc; Electrophoresis, Polyacrylamide Gel; Humans; Immunodiffusion; Immunoelectrophoresis; Immunoglobulin Fab Fragments; Immunoglobulin Fc Fragments; Immunoglobulin Fragments; Immunoglobulin M; Pepsin A; Protein Binding; Species Specificity; Swine; Time Factors; Trypsin; Ultracentrifugation; Urea | 1974 |
The reaction of creatine kinase with dithiobisnitrobenzoic acid. Formation of derivatives of the enzyme.
Topics: Adenosine Diphosphate; Adenosine Triphosphatases; Adenosine Triphosphate; Animals; Benzoates; Binding Sites; Carbon Isotopes; Catechol Oxidase; Chemical Phenomena; Chemistry; Creatine Kinase; Disulfides; Dithiothreitol; Enzyme Activation; Macromolecular Substances; Magnetic Resonance Spectroscopy; Manganese; Muscles; Nitro Compounds; Protein Binding; Rabbits; Sulfhydryl Compounds; Tyrosine; Ultracentrifugation; Urea | 1971 |
Studies on the egg-membrane lysin of Tegula pfeifferi. The reaction mechanism of the egg-membrane lysin.
Topics: Amino Acids; Animals; Arginine; Binding Sites; Carbohydrates; Cell Membrane; Chromatography, Gel; Dithiothreitol; Egg Proteins; Female; Glycosaminoglycans; Hydrogen-Ion Concentration; Kinetics; Molecular Weight; Mucoproteins; Ovum; Protein Binding; Seawater; Snails; Solubility; Spectrophotometry, Ultraviolet; Urea | 1974 |
The cardiac auto-immune system. V. Human heart antigens cross-reactive with rabbit anti-rabbit heart auto-antibodies.
Topics: Aminopeptidases; Animals; Antigen-Antibody Reactions; Antigens; Autoantibodies; Catalase; Cross Reactions; Deoxyribonucleases; Dithiothreitol; Esterases; Glucuronidase; Hemagglutination Inhibition Tests; Hemagglutination Tests; Hexosaminidases; Humans; Immunodiffusion; Myocardium; Organ Specificity; Peptide Hydrolases; Phosphates; Phospholipids; Phosphoric Monoester Hydrolases; Potassium; Quaternary Ammonium Compounds; Rabbits; Ribonucleases; Sulfates; Tissue Extracts; Urea | 1972 |
Dissociation and reassociation of infectious poliovirus particles.
Topics: Centrifugation, Density Gradient; Dithiothreitol; HeLa Cells; Humans; Leucine; Mercaptoethanol; Pancreas; Peptides; Poliovirus; Ribonucleases; RNA, Viral; Tritium; Urea; Uridine; Viral Plaque Assay; Viral Proteins | 1972 |
Changes in sulfhydryl groups of honeybee glyceraldehyde phosphate dehydrogenase associated with generation of the intermediate plateau in its saturation kinetics.
Topics: Acetates; Animals; Bees; Benzoates; Bromine; Carbon Radioisotopes; Disulfides; Dithiothreitol; Drug Stability; Glyceraldehyde; Glyceraldehyde-3-Phosphate Dehydrogenases; Kinetics; Muscles; NAD; Nitro Compounds; Organophosphorus Compounds; Protein Denaturation; Rabbits; Sulfhydryl Compounds; Urea | 1974 |
Homology between enterotoxin protein and spore structural protein in Clostridium perfringens type A.
Topics: Animals; Bacterial Proteins; Chromatography, Gel; Chromatography, Ion Exchange; Clostridium perfringens; Dithiothreitol; Electrophoresis, Disc; Hydrogen-Ion Concentration; Immunodiffusion; Mercaptoethanol; Rabbits; Solubility; Species Specificity; Spores; Toxins, Biological; Urea | 1973 |
Near ultraviolet circular dichroism and absorption spectra of chicken ovomucoid and acetylated derivatives at 297 and 77 degrees K.
Topics: Acetylation; Animals; Chickens; Chromatography; Chromatography, Gel; Chromatography, Ion Exchange; Circular Dichroism; Disulfides; Dithiothreitol; Egg Proteins; Guanidines; Hot Temperature; Imidazoles; Molecular Weight; Mucoproteins; Protein Conformation; Silicon Dioxide; Spectrophotometry, Ultraviolet; Structure-Activity Relationship; Temperature; Tyrosine; Urea | 1974 |
Subunit structure of testicular hyaluronidase.
Topics: Amino Acid Sequence; Amino Acids; Animals; Binding Sites; Cattle; Chromatography, Gel; Chromatography, Ion Exchange; Dansyl Compounds; Dialysis; Dithiothreitol; Electrophoresis, Disc; Hyaluronoglucosaminidase; Kinetics; Male; Molecular Weight; Oligosaccharides; Oxidation-Reduction; Peptide Fragments; Periodic Acid; Protein Binding; Ribonucleases; Spectrophotometry; Spectrophotometry, Ultraviolet; Testis; Tritium; Trypsin; Urea | 1974 |
A rubredoxin-like mononuclear FeS4 derivative of adrenal iron-sulfur protein (adrenodoxin).
Topics: Adrenal Glands; Apoproteins; Binding Sites; Chromatography, DEAE-Cellulose; Colorimetry; Dithiothreitol; Drug Stability; Electron Spin Resonance Spectroscopy; Ferredoxins; Half-Life; Iron; Metalloproteins; Phenols; Protein Binding; Protein Conformation; Rubredoxins; Selenium; Spectrophotometry; Spectrophotometry, Ultraviolet; Sulfides; Sulfonic Acids; Sulfur; Temperature; Time Factors; Urea | 1974 |
Physical properties of glucose 6-phosphate dehydrogenase from Neurospora crassa.
Topics: Acetamides; Alkylation; Chemical Phenomena; Chemistry; Chromatography, Gel; Detergents; Dithiothreitol; Electrophoresis; Electrophoresis, Disc; Glucosephosphate Dehydrogenase; Guanidines; Hydrochloric Acid; Iodoacetates; Isoelectric Focusing; Macromolecular Substances; Microscopy, Electron; Molecular Biology; Molecular Weight; NADP; Neurospora; Neurospora crassa; Oxidation-Reduction; Sulfates; Ultracentrifugation; Urea | 1971 |
Lung parenchymal elastin isolated by non-degradative means.
Topics: Amino Acids; Animals; Binding Sites; Connective Tissue; Disulfides; Dithiothreitol; Elastin; Evaluation Studies as Topic; Galactosamine; Glucosamine; Guanidines; Hydrogen-Ion Concentration; Lung; Methods; Protein Binding; Protein Conformation; Sodium Dodecyl Sulfate; Species Specificity; Urea | 1974 |
Triton X-100-4 M urea as an extraction medium for membrane proteins. I. Purification of chloroplast cytochrome b559.
Topics: Ascorbic Acid; Binding Sites; Cell Membrane; Chloroplasts; Chromatography, DEAE-Cellulose; Cytochromes; Dithiothreitol; Drug Stability; Electrophoresis, Disc; Ethanol; Glycerol; Hydrogen-Ion Concentration; Macromolecular Substances; Nitrogen; Oxidation-Reduction; Plants; Polyethylene Glycols; Protein Binding; Protein Denaturation; Spectrophotometry; Spectrophotometry, Ultraviolet; Surface-Active Agents; Time Factors; Ultrasonics; Urea | 1974 |
Analysis of the protein composition of yeast ribosomal subunits by two-dimensional polyacrylamide gel electrophoresis.
Topics: Centrifugation, Density Gradient; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Fungal Proteins; Hydrogen-Ion Concentration; Osmolar Concentration; Ribosomes; Saccharomyces; Urea | 1974 |
The effect of denaturing conditions on the activity of rabbit muscle amylo-1,6-glucosidase-oligo-1,4 leads to 1,4-glucantransferase.
Topics: Animals; Carbon Radioisotopes; Dextrans; Dialysis; Dithiothreitol; Freezing; Glucosidases; Glucosyltransferases; Hydrogen-Ion Concentration; Kinetics; Muscles; Oligosaccharides; Protein Denaturation; Rabbits; Temperature; Time Factors; Urea | 1974 |
The effect of sodium hydroxide or dithiothreitol-urea on spores of germination mutants of Clostridium bifermentans.
Topics: Alanine; Anaerobiosis; Arginine; Clostridium; Dithiothreitol; Hot Temperature; Hydrogen-Ion Concentration; Lactates; Mutation; Phenylalanine; Sodium Hydroxide; Spectrophotometry; Spores, Bacterial; Stereoisomerism; Urea | 1974 |
Nuclear triiodothyronine-binding protein: partial characterization and binding to chromatin.
Topics: Animals; Cell Nucleus; Chloromercuribenzoates; Chromatin; Dithiothreitol; Electrophoresis, Starch Gel; Iodine Radioisotopes; Liver; Pronase; Protein Binding; Protein Denaturation; Proteins; Rats; Triiodothyronine; Urea | 1974 |
Tryptophan fluorescence study of conformational transitions of the oxidized and reduced form of thioredoxin.
Topics: Amino Acid Sequence; Amino Acids; Bacterial Proteins; Dioxins; Disulfides; Dithiothreitol; Escherichia coli; Ethanol; Guanidines; Hydrogen-Ion Concentration; Oxidation-Reduction; Plant Proteins; Protein Conformation; Protein Denaturation; Saccharomyces; Species Specificity; Spectrometry, Fluorescence; Tryptophan; Urea | 1972 |
Reversible inactivation and reactivation of thymidylate synthetase.
Topics: Dithiothreitol; Escherichia coli; Mercaptoethanol; Methylation; Transferases; Uracil Nucleotides; Urea | 1972 |
Evidence for nucleation in the folding of reduced hen egg lysozyme.
Topics: Animals; Autolysis; Chickens; Chromatography, Paper; Chymotrypsin; Copper; Dithiothreitol; Female; Hydrolysis; Muramidase; Ovum; Oxidation-Reduction; Pepsin A; Protein Conformation; Sulfhydryl Compounds; Urea | 1973 |
Enthalpy of reduction of disulfide cross-links in denatured lysozyme.
Topics: Calorimetry; Disulfides; Dithiothreitol; Glutathione; Guanidines; Microchemistry; Muramidase; Ovalbumin; Oxidation-Reduction; Pepsin A; Protein Conformation; Protein Denaturation; Protein Hydrolysates; Spectrophotometry, Ultraviolet; Thermodynamics; Urea | 1973 |
Subunits of RNA polymerase in function and structure. 3. Accumulation of the intermediate alpha 2beta in the subunit assembly of Escherichia coli RNA polymerase by treatment with cyanate.
Topics: Carbon Isotopes; Centrifugation, Density Gradient; Chloromercuribenzoates; Culture Media; Cyanates; Dithiothreitol; DNA-Directed RNA Polymerases; Enzyme Activation; Escherichia coli; Hydrogen-Ion Concentration; Magnesium; Protein Binding; Time Factors; Tritium; Urea | 1973 |
Comparison of procedures for extracting transfer RNA from spores of Bacillus.
Topics: Aluminum Silicates; Bacillus megaterium; Bacillus subtilis; Carbonates; Chemical Precipitation; Chromatography, Gel; Dithiothreitol; Edetic Acid; Ethanol; Hydrogen-Ion Concentration; Kinetics; Mercaptoethanol; Methods; Muramidase; Phenols; Ribonucleases; RNA, Transfer; Spores; Spores, Bacterial; Thioglycolates; Urea | 1972 |
Subunit structure of Helix pomatia A hemagglutinin.
Topics: Alkylation; Antibodies; Binding Sites, Antibody; Carbon Radioisotopes; Chemical Precipitation; Chromatography, Gel; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Guanidines; Hemagglutination Inhibition Tests; Immunoelectrophoresis; Iodine Radioisotopes; Iodoacetates; Molecular Weight; Oxidation-Reduction; Peptides; Snails; Trypsin; Ultracentrifugation; Urea | 1972 |
Immobilized isocrite dehydrogenase: some aspects of its catalytic, chemical and immunological properties.
Topics: Animals; Antibodies; Antigen-Antibody Complex; Azotobacter; Benzoates; Binding Sites; Catalysis; Chemical Phenomena; Chemistry, Physical; Cyanides; Cyanogen Bromide; Disulfides; Dithiothreitol; Enzyme Activation; Isocitrate Dehydrogenase; Kinetics; Lysine; Magnesium; Nitro Compounds; Polysaccharides; Protein Conformation; Rabbits; Trypsin; Urea | 1972 |
In vitro formation of apparent covalent complexes between L-triiodothyronine and plasma protein.
Topics: Blood Proteins; Chromatography, Paper; Copper; Dithiothreitol; Flavin Mononucleotide; Glutathione; Humans; In Vitro Techniques; Iodine Isotopes; Pronase; Propylthiouracil; Protein Binding; Sodium Dodecyl Sulfate; Thiosulfates; Triiodothyronine; Urea | 1973 |
An improved haptoglobin subtyping procedure using polyacrylamide gel electrophoresis. Haptoglobin gene frequency distribution among a group of blood bank donors.
Topics: Alkylation; Chromatography, DEAE-Cellulose; Densitometry; Dithiothreitol; Electrophoresis, Disc; Electrophoresis, Polyacrylamide Gel; Genes; Haptoglobins; Oxidation-Reduction; Polymorphism, Genetic; Urea | 1973 |
Sulfhydryl and disulfide groups of platelet membranes. II. Determination of disulfide groups.
Topics: Benzoates; Blood Platelets; Cell Membrane; Chloromercuribenzoates; Disulfides; Dithiothreitol; Humans; Hydrogen-Ion Concentration; Male; Mercury Isotopes; Methods; Nitro Compounds; Spectrophotometry; Time Factors; Urea | 1973 |
Direct spectrophotometric measurement of the rate of reduction of disulfide bonds. The reactivity of the disulfide bonds of bovine -lactalbumin.
Topics: Animals; Cattle; Disulfides; Dithiothreitol; Glutathione; Kinetics; Lactalbumin; Mercaptoethanol; Oxidation-Reduction; Protein Conformation; Protein Denaturation; Ribonucleases; Serum Albumin, Bovine; Spectrophotometry, Ultraviolet; Temperature; Urea | 1973 |
Isolation of a major hydrophobic protein of the mitochondrial inner membrane.
Topics: Animals; Chromatography, Gel; Dithiothreitol; Electron Transport; Electrophoresis, Polyacrylamide Gel; Lysophosphatidylcholines; Membranes; Mitochondria; Molecular Weight; Proteins; Sodium Dodecyl Sulfate; Solubility; Spectrophotometry, Ultraviolet; Urea; Water | 1973 |
Cytochrome P-450cam. II. Interconversion with P-420.
Topics: Acetone; Camphor; Carbon Monoxide; Chloromercuribenzoates; Cysteine; Cytochrome P-450 Enzyme Inhibitors; Cytochrome P-450 Enzyme System; Deoxycholic Acid; Dithiothreitol; Homocysteine; Mercaptoethanol; Oxidation-Reduction; Pseudomonas; Spectrophotometry; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds; Thioglycolates; Urea | 1974 |
Basic structure of mouse histocompatibility antigens.
Topics: Animals; Azirines; Cell Membrane; Chromatography, Gel; Chromatography, Ion Exchange; Chromium Radioisotopes; Dithiothreitol; Electrophoresis, Disc; Histocompatibility Antigens; Immunization; Immunoelectrophoresis; Isoantigens; Isoelectric Focusing; Lymphocytes; Lymphoma; Mice; Mice, Inbred BALB C; Molecular Weight; Papain; Solubility; Spectrophotometry; Spleen; Urea | 1974 |
Scanning density gradient isoelectric focusing of Staphylococcus aureus enterotoxins B and C.
Topics: Centrifugation, Density Gradient; Dithiothreitol; Enterotoxins; Hydrogen-Ion Concentration; Isoelectric Focusing; Staphylococcus; Sucrose; Urea | 1974 |
Separation and characterisation of subunits of histidine decarboxylase from Micrococcus sp.n.
Topics: Amino Acid Sequence; Amino Acids; Carbon Radioisotopes; Carboxy-Lyases; Chromatography, Gel; Chromatography, Paper; Dithiothreitol; Electrophoresis, Paper; Histidine; Iodoacetates; Macromolecular Substances; Micrococcus; Molecular Weight; Oxidation-Reduction; Peptide Fragments; Spectrophotometry, Ultraviolet; Tosyl Compounds; Trypsin; Urea | 1974 |
Isolation and characterization of subunits of human Clq.
Topics: Amino Acids; Blood Proteins; Complement System Proteins; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Fucose; Galactose; Glucose; Glycoproteins; Hexosamines; Humans; Iodoacetates; Mannose; Molecular Weight; Neuraminic Acids; Oxidation-Reduction; Sodium Dodecyl Sulfate; Urea | 1974 |
Dependence of staphylococcal clumping activity of fibrinogen on conformation.
Topics: Binding Sites; Dialysis; Dithiothreitol; Fibrinogen; Humans; Hydrogen-Ion Concentration; Methylcellulose; Protein Conformation; Protein Denaturation; Staphylococcus; Urea | 1974 |
Immobilization of urease by thiol-disulphide interchange with concomitant purification.
Topics: Absorption; Chemical Phenomena; Chemistry; Disulfides; Dithiothreitol; Gels; Glutathione; Hydrolysis; Polysaccharides; Pyridines; Sulfides; Temperature; Time Factors; Urea; Urease | 1974 |
Stabilization of native structure by the closed disulfide loop of staphylococcal enterotoxin B.
Topics: Acetamides; Animals; Binding Sites; Biological Assay; Chromatography, Gel; Disulfides; Dithiothreitol; Drug Stability; Enterotoxins; Guanidines; Iodoacetates; Kinetics; Oxidation-Reduction; Protein Binding; Protein Conformation; Protein Denaturation; Rabbits; Spectrophotometry, Ultraviolet; Staphylococcus; Sulfhydryl Compounds; Trypsin; Urea; Viscosity | 1974 |
Solvent perturbation difference spectra of a purified sheep antibody.
Topics: Animals; Antibodies; Dimethyl Sulfoxide; Dithiothreitol; Ethylenes; Glycerol; Glycols; Models, Chemical; Polyethylene Glycols; Precipitin Tests; Sheep; Solvents; Spectrophotometry; Tryptophan; Tyrosine; Urea | 1974 |
Molecular probes of spermatozoan structures.
Topics: Agglutination; Animals; Binding Sites; Cell Movement; Centrifugation, Density Gradient; Concanavalin A; Detergents; Dithiothreitol; Fluoresceins; Guanidines; Guinea Pigs; Humans; Male; Mercaptoethanol; Mice; Microscopy, Phase-Contrast; Naphthalenes; Protein Denaturation; Rabbits; Rats; Receptors, Drug; Spermatozoa; Sulfonic Acids; Surface-Active Agents; Trypsin; Urea | 1971 |
Escherichia coli B N 5 -methyltetrahydrofolate-homocysteine cobalamin methyltransferase: gel-filtration behavior of apoenzyme and holoenzymes.
Topics: Alkylation; Carbon Isotopes; Chemical Phenomena; Chemistry, Physical; Chromatography, Gel; Dithiothreitol; Drug Stability; Escherichia coli; Folic Acid; Homocysteine; Hydroxocobalamin; Macromolecular Substances; Mathematics; Methylation; Methyltransferases; Molecular Weight; Tritium; Urea; Vitamin B 12 | 1971 |
Molecular weight, buoyant density, and composition of active subunits of rat liver ribosomes.
Topics: Animals; Centrifugation, Density Gradient; Chemical Phenomena; Chemistry, Physical; Dialysis; Dithiothreitol; Edetic Acid; Formaldehyde; Liver; Macromolecular Substances; Magnesium; Male; Molecular Weight; Orotic Acid; Phosphates; Potassium; Potassium Chloride; Proteins; Rats; Ribosomes; RNA; RNA, Ribosomal; Time Factors; Tritium; Urea | 1971 |
Reconstitution of bacterial spore coat layers in vitro.
Topics: Bacillus cereus; Bacterial Proteins; Carbon Isotopes; Cell Wall; Centrifugation, Density Gradient; Cystine; Dialysis; Diatrizoate; Dithiothreitol; Hydrogen-Ion Concentration; Isoleucine; Microscopy, Electron; Microscopy, Phase-Contrast; Muramidase; Spores; Spores, Bacterial; Tritium; Urea | 1971 |
Structural proteins in the egg-shell of the oriental garden cricket, Gryllus mitratus.
Topics: Amino Acids; Animals; Calcium; Chemical Precipitation; Cystine; Disulfides; Dithiothreitol; Edetic Acid; Electrophoresis; Female; Gels; Insecta; Magnesium; Molecular Weight; Ovum; Phosphates; Phosphoproteins; Proteins; Serine; Sodium; Ultracentrifugation; Urea | 1971 |
Noncovalent interactions between immunoglobulin polypeptide chains. Stability to dissociation by denaturants.
Topics: Acetamides; Alkylation; Chromatography, Gel; Dithiothreitol; Drug Stability; Electrophoresis; Humans; Immunoglobulin A; Immunoglobulin G; Immunoglobulin M; Immunoglobulins; Iodine Isotopes; Iodoacetates; Macromolecular Substances; Mercaptoethanol; Myeloma Proteins; Oxidation-Reduction; Protein Denaturation; Urea | 1972 |
The relationship between reticulocyte polyribosome structure and function.
Topics: Adenosine Triphosphate; Animals; Carbon Isotopes; Centrifugation, Density Gradient; Chloromercuribenzoates; Dithiothreitol; Ethylmaleimide; Guanosine Triphosphate; Macromolecular Substances; Phosphoenolpyruvate; Phosphorus Isotopes; Protein Biosynthesis; Protein Denaturation; Pyruvate Kinase; Rabbits; Reticulocytes; Ribosomes; Urea; Valine | 1972 |
The structural proteins of rubella virus.
Topics: Amino Acids; Carbohydrates; Carbon Isotopes; Centrifugation, Density Gradient; Dithiothreitol; Electrophoresis, Disc; Glycoproteins; Iodoacetates; Molecular Weight; Peptides; Rubella virus; Sodium Dodecyl Sulfate; Sucrose; Tritium; Urea; Viral Proteins; Virus Cultivation | 1972 |
Sedimentation analyses of native silk fibroin in urea and guanidine.HCl.
Topics: Alkylation; Centrifugation, Density Gradient; Chemical Phenomena; Chemistry, Physical; Chromatography, Gel; Disulfides; Dithiothreitol; Fibroins; Guanidines; Macromolecular Substances; Mathematics; Molecular Weight; Oxidation-Reduction; Ultracentrifugation; Urea | 1972 |
Coenzyme B 12 dependent propanediol dehydratase system. Nature of cobalamin binding and some properties of apoenzyme-coenzyme B 12 analog complexes.
Topics: Apoproteins; Chloromercuribenzoates; Chromatography, Gel; Cobamides; Coenzymes; Cyanides; Dithiothreitol; Drug Stability; Enterobacter; Hot Temperature; Hydro-Lyases; Hydrogen-Ion Concentration; Hydroxocobalamin; Methylation; Potassium; Propylene Glycols; Protein Binding; Spectrophotometry; Urea; Vitamin B 12 | 1972 |
The binding of the peroxidase oxidation products of indole-3-acetic acid to histone.
Topics: Animals; Arginine; Carbon Isotopes; Cattle; Chemical Phenomena; Chemistry; Dithiothreitol; Electrophoresis; Glutathione; Histones; Indoleacetic Acids; Indoles; Macromolecular Substances; Oxidation-Reduction; Peroxidases; Plants; Protein Binding; Spectrophotometry; Sulfhydryl Compounds; Thymus Gland; Ultraviolet Rays; Urea | 1972 |
Chemical and mechanical dissolution of human senile cataracts in vitro.
Topics: Adult; Age Factors; Aged; Cataract; Color; Corneal Opacity; Dithiothreitol; Female; Guanidines; Hardness; Humans; Lens, Crystalline; Male; Middle Aged; Sodium Chloride; Stress, Mechanical; Surface-Active Agents; Urea; Vision Disorders | 1972 |
Effects on lethality of toxins in venom from the scorpion Centruroides sculpturatus by group specific reagents.
Topics: Amino Acids; Animals; Antitoxins; Azo Compounds; Benzene Derivatives; Chloromercuribenzoates; Cysteamine; Cysteine; Dithiothreitol; Iodoacetates; Iodobenzoates; Methyl Ethers; Mice; Proteins; Scorpions; Sulfhydryl Reagents; Sulfonic Acids; Thioglycolates; Toxins, Biological; Urea; Venoms | 1972 |
An immunoglobulin light chain of subgroup III-1 of lambda type with a free sulfhydryl group.
Topics: Amino Acid Sequence; Ammonium Sulfate; Bence Jones Protein; Chemical Precipitation; Chromatography, Gel; Cysteine; Dansyl Compounds; Dithiothreitol; Electrophoresis; Guanidines; Humans; Immunoglobulin G; Immunoglobulins; Iodine Isotopes; Iodoacetates; Methylation; Multiple Myeloma; Myeloma Proteins; Oxidation-Reduction; Peptides; Protein Denaturation; Sulfhydryl Compounds; Tryptophan; Tyrosine; Urea; Valine | 1971 |
One-step preparation of the polypeptide chains of -crystallin.
Topics: Chromatography, DEAE-Cellulose; Crystallins; Dialysis; Dithiothreitol; Electrophoresis; Macromolecular Substances; Methods; Spectrophotometry; Urea | 1971 |
Gel electrophoresis of mucous glycoproteins. II. Effect of physical deaggregation and disulfide-bond cleavage.
Topics: Acrylamides; Animals; Blood Proteins; Cattle; Chromatography; Detergents; Disulfides; Dithiothreitol; Dogs; Electrophoresis; Fibrinogen; Gels; Humans; Macromolecular Substances; Methods; Molecular Weight; Mucins; Oxidation-Reduction; Polysaccharides; Sheep; Submandibular Gland; Sulfuric Acids; Swine; Trachea; Urea | 1971 |
A new NMR technique to study disulfide reduction: comparison of lysozyme and -lactalbumin.
Topics: Albumins; Chlorides; Computers; Disulfides; Dithiothreitol; Magnetic Resonance Spectroscopy; Methods; Muramidase; Oxidation-Reduction; Protein Conformation; Radioisotopes; Sulfides; Urea | 1971 |
Cyanate formation in solutions of urea. II. Effect of urea on the eye lens protein -crystallin.
Topics: Animals; Cattle; Chromatography, Ion Exchange; Crystallins; Cyanates; Dithiothreitol; Electrophoresis; Hydrogen-Ion Concentration; Macromolecular Substances; Potassium; Temperature; Time Factors; Urea | 1971 |
Reduction of chymotrypsin A by dithiothreitol.
Topics: Acrylates; Chemical Phenomena; Chemistry; Chloromercuribenzoates; Chymotrypsin; Cinnamates; Dithiothreitol; Hydrogen-Ion Concentration; Indoles; Oxidation-Reduction; Urea | 1971 |
Purification of chloroplast cytochrome b 559 .
Topics: Chemical Precipitation; Chloroplasts; Chromatography, DEAE-Cellulose; Cold Temperature; Cytochromes; Dithiothreitol; Electrophoresis, Disc; Ethanol; Methods; Oxidation-Reduction; Plants; Quaternary Ammonium Compounds; Spectrophotometry; Sulfates; Surface-Active Agents; Urea | 1971 |
Hybridization of bacterial luciferase with a variant produced by chemical modification.
Topics: Acylation; Anhydrides; Chemical Phenomena; Chemistry; Chromatography, Ion Exchange; Dialysis; Dithiothreitol; Electrophoresis; Hybridization, Genetic; Luciferases; Lysine; Macromolecular Substances; Photobacterium; Photometry; Protein Denaturation; Structure-Activity Relationship; Succinates; Ultracentrifugation; Urea | 1971 |
Analytical scanning isoelectrofocusing. 5. Separation of glycinin subunits in urea-dithiothreitol media.
Topics: Detergents; Dithiothreitol; Glycine max; Hydrogen-Ion Concentration; Isoelectric Focusing; Kinetics; Methods; Osmolar Concentration; Peptides; Plant Proteins; Solubility; Spectrophotometry; Sucrose; Ultraviolet Rays; Urea | 1971 |
Fatty acid synthetase from lactating rat mammary gland. 3. Dissociation and reassociation.
Topics: Animals; Benzoates; Carbon Isotopes; Centrifugation, Density Gradient; Chloromercuribenzoates; Cold Temperature; Disulfides; Dithiothreitol; Drug Stability; Enzyme Activation; Fatty Acid Synthases; Female; Lactation; Liver; Macromolecular Substances; Male; Mammary Glands, Animal; Nitro Compounds; Pregnancy; Protein Denaturation; Rats; Sulfhydryl Compounds; Temperature; Time Factors; Urea | 1971 |
A soluble mitochondrial ATP synthetase complex catalyzing ATP-phosphate and ATP-ADP exchange.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Chromatography, DEAE-Cellulose; Chromatography, Gel; Dithiothreitol; Mercury; Mitochondria; Oligomycins; Organometallic Compounds; Oxidative Phosphorylation; Phosphotransferases; Sulfonic Acids; Uncoupling Agents; Urea | 1971 |
Isolation of non-identical polypeptide chains of -crystallin.
Topics: Alkylation; Amino Acids; Animals; Cattle; Chemical Precipitation; Chromatography, Gel; Chromatography, Ion Exchange; Crystallins; Cysteine; Detergents; Disulfides; Dithiothreitol; Electrophoresis; Ethanol; Formates; Hydrogen-Ion Concentration; Hydrolysis; Iodoacetates; Macromolecular Substances; Mercaptoethanol; Oxidation-Reduction; Peptides; Sulfhydryl Compounds; Sulfuric Acids; Ultracentrifugation; Urea | 1969 |
Subunit interactions in gamma-glutamyl transpeptidase. Reconstitution of the active species from isolated subunits.
Topics: Animals; Dithiothreitol; gamma-Glutamyltransferase; Kidney; Macromolecular Substances; Molecular Weight; Rats; Urea | 1981 |
Subunit interactions in human plasma fibronectin.
Topics: Dithiothreitol; Fibrinolysin; Fibronectins; Humans; Light; Macromolecular Substances; Molecular Weight; Osmolar Concentration; Scattering, Radiation; Time Factors; Urea | 1984 |
Dissociation of CNPase dimer into catalytically active monomers by 1,4-dithiothreitol and urea. Protein-promoted reassembly.
Topics: 2',3'-Cyclic-Nucleotide Phosphodiesterases; Animals; Cattle; Dithiothreitol; Enzyme Activation; Molecular Weight; Phosphoric Diester Hydrolases; Urea | 1982 |
An attempt to resolve all the various proteins in a single human cell type by two-dimensional electrophoresis: I. Extraction of all cell proteins.
Topics: Cell Line; Dithiothreitol; Electrophoresis; Humans; Isoelectric Focusing; Laryngeal Neoplasms; Mercaptoethanol; Octoxynol; Polyethylene Glycols; Proteins; Quaternary Ammonium Compounds; Sodium Dodecyl Sulfate; Urea | 1984 |
The effect of mucolytic agents on the rheologic and transport properties of canine tracheal mucus.
Topics: Acetylcysteine; Animals; Carbocysteine; Cysteine; Dithiothreitol; Dogs; Elasticity; Expectorants; In Vitro Techniques; Mucus; Potassium Iodide; Rana catesbeiana; Rheology; Trachea; Urea; Viscosity | 1980 |
Removal of sulfhydryl groups with 1,3,4,6-tetrachloro-3a, 6a- diphenylglycoluril: application to the assay of protein in the presence of thiol reagents.
Topics: Chemical Phenomena; Chemistry; Dithiothreitol; Imidazoles; Indicators and Reagents; Kinetics; Methods; Proteins; Sulfhydryl Compounds; Urea | 1981 |
Autolytic fragmentation of complement components C3 and C4 under denaturing conditions, a property shared with alpha 2-macroglobulin.
Topics: Complement C3; Complement C4; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Hot Temperature; Humans; Kinetics; Peptide Fragments; Protein Denaturation; Sodium Dodecyl Sulfate; Urea | 1981 |
Kinetic evidence for active monomers during the reassembly of denatured creatine kinase.
Topics: Animals; Brain; Creatine Kinase; Dithiothreitol; Kinetics; Macromolecular Substances; Muscles; Protein Denaturation; Rabbits; Spectrometry, Fluorescence; Urea | 1981 |
Electron microscopic studies of rat sperm heads treated with urea, dithiothreitol, and micrococcal nuclease.
Topics: Animals; Cell Nucleus; Chromatin; Dithiothreitol; Male; Micrococcal Nuclease; Microscopy, Electron; Rats; Sperm Head; Spermatozoa; Urea | 1981 |
Noncatalytic subunits of human blood plasma coagulation factor XIII. Preparation and partial characterization of modified forms.
Topics: Amino Acids; Cyanogen Bromide; Dithiothreitol; Factor XIII; Humans; Macromolecular Substances; Molecular Weight; Peptide Fragments; Protein Binding; Urea | 1980 |
Expression of fungal Mn peroxidase in E. coli and refolding to yield active enzyme.
Topics: Base Sequence; Basidiomycota; Calcium; Dithiothreitol; DNA, Complementary; Escherichia coli; Gene Expression; Gene Transfer Techniques; Glutathione; Hemin; Hydrogen Peroxide; Manganese; Molecular Sequence Data; Oxidation-Reduction; Peroxidases; Protein Folding; Recombinant Proteins; Solubility; Urea | 1995 |
Mass spectrometric composition, molecular mass and oxygen binding of Macrobdella decora hemoglobin and its tetramer and monomer subunits.
Topics: Allosteric Regulation; Animals; Cations; Centrifugation, Density Gradient; Chromatography, High Pressure Liquid; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Hemoglobins; Hydrogen-Ion Concentration; Leeches; Mass Spectrometry; Molecular Weight; Oxygen; Peptides; Protein Conformation; Protein Denaturation; Urea | 1995 |
Role of disulfide linkages in structure and activity of proteinase inhibitor from horsegram (Dolichos biflorus).
Topics: Disulfides; Dithiothreitol; Fabaceae; Guanidine; Guanidines; Oxidation-Reduction; Plants, Medicinal; Protease Inhibitors; Protein Folding; Urea | 1995 |
The effect of denaturants on PDI conformation and activity.
Topics: Animals; Cattle; Dithiothreitol; Edetic Acid; Isomerases; Kinetics; Protein Conformation; Protein Denaturation; Protein Disulfide-Isomerases; Spectrometry, Fluorescence; Urea | 1995 |
The refolding of hen egg white riboflavin binding protein.
Topics: Animals; Carrier Proteins; Chickens; Dithiothreitol; Edetic Acid; Female; Guanidine; Guanidines; Membrane Transport Proteins; Phosphorylation; Protein Conformation; Protein Denaturation; Protein Folding; Riboflavin; Spectrometry, Fluorescence; Tryptophan; Urea | 1995 |
Expression of a cDNA encoding the wheat CM16 protein in Escherichia coli.
Topics: Amino Acids; Base Sequence; Cloning, Molecular; Dithiothreitol; DNA Primers; DNA, Complementary; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Macromolecular Substances; Molecular Sequence Data; Molecular Weight; Plant Proteins; Plasmids; Recombinant Proteins; Restriction Mapping; Seeds; Triticum; Urea | 1994 |
Denatured state of ovalbumin in high concentrations of urea as evaluated by disulfide rearrangement analysis.
Topics: Amino Acid Sequence; Animals; Chickens; Chromatography, High Pressure Liquid; Circular Dichroism; Cysteine; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Female; Fluorescent Dyes; Kinetics; Molecular Sequence Data; Ovalbumin; Peptide Fragments; Peptide Mapping; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Urea | 1994 |
AJ-FS1 monoclonal antibody detects a novel group of non-glycosylated antigens within the human sperm tail fibrous sheath.
Topics: Antibodies, Monoclonal; Antibody Specificity; Antigens; Blotting, Western; Dithiothreitol; Fluorescent Antibody Technique; Glycosylation; Humans; Male; Microscopy, Immunoelectron; Periodic Acid; Sperm Tail; Spermatogenesis; Urea | 1994 |
Cell-free formation of disulfide-bonded multimer from isolated plasma fibronectin in the presence of a low concentration of SH reagent under a physiological condition.
Topics: Animals; Calpain; Cattle; Cell Adhesion; Cell-Free System; Chromatography, High Pressure Liquid; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Extracellular Matrix; Fibronectins; Fibrosarcoma; Molecular Weight; Polymers; Protein Binding; Tumor Cells, Cultured; Urea | 1994 |
Mutations of noncatalytic sulfhydryl groups influence the stability, folding, and oxidative susceptibility of rhodanese.
Topics: Amino Acid Sequence; Base Sequence; Cloning, Molecular; Cysteine; Dithiothreitol; Enzyme Stability; Escherichia coli; Kinetics; Models, Structural; Molecular Sequence Data; Mutagenesis, Site-Directed; Oligodeoxyribonucleotides; Oxidation-Reduction; Point Mutation; Protein Conformation; Protein Folding; Recombinant Proteins; Thiosulfate Sulfurtransferase; Urea | 1994 |
Human thyroid peroxidase: autoantibody recognition depends on the natural conformation.
Topics: Autoantibodies; Autoantigens; Dithiothreitol; Enzyme-Linked Immunosorbent Assay; Hot Temperature; Humans; In Vitro Techniques; Iodide Peroxidase; Protein Conformation; Sodium Dodecyl Sulfate; Thyroiditis, Autoimmune; Urea | 1993 |
Isolation and characterization of urease from Aspergillus niger.
Topics: Anions; Aspergillus niger; Cations; Dithiothreitol; Edetic Acid; Enzyme Stability; Enzymes, Immobilized; Hot Temperature; Molecular Weight; Urea; Urease | 1993 |
Overexpression of a Rhizopus delemar lipase gene in Escherichia coli.
Topics: Amino Acid Sequence; Base Sequence; Cloning, Molecular; Cysteine; Cystine; Dithiothreitol; DNA; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Gene Expression; Hydrogen-Ion Concentration; Lipase; Molecular Sequence Data; Mutagenesis, Site-Directed; Oxidation-Reduction; Protein Folding; Recombinant Proteins; Rhizopus; Solubility; Urea | 1993 |
Structural characterization of the disulfide folding intermediates of bovine alpha-lactalbumin.
Topics: Anilino Naphthalenesulfonates; Animals; Calcium; Cattle; Chromatography, High Pressure Liquid; Circular Dichroism; Disulfides; Dithiothreitol; Edetic Acid; Electrophoresis, Polyacrylamide Gel; Fluorescence; Fluorescent Dyes; Lactalbumin; Naphthalenesulfonates; Oxidation-Reduction; Protein Folding; Urea | 1993 |
Properties and stabilization of an extracellular alpha-glucosidase from the extremely thermophilic archaebacteria Thermococcus strain AN1: enzyme activity at 130 degrees C.
Topics: alpha-Glucosidases; Archaea; Carbohydrate Metabolism; Carbohydrate Sequence; Dithiothreitol; Enzyme Activation; Enzyme Inhibitors; Enzyme Stability; Hot Temperature; Hydrogen-Ion Concentration; Kinetics; Metals; Molecular Sequence Data; Molecular Weight; Protein Conformation; Protein Denaturation; Sodium Dodecyl Sulfate; Sorbitol; Substrate Specificity; Urea | 1996 |
Performance of urea-sensitive enzyme field effect transistors: influence of the storage conditions.
Topics: Azides; Biosensing Techniques; Buffers; Chelating Agents; Dithiothreitol; Edetic Acid; Glycerol; Humans; Hydrogen-Ion Concentration; Mutagens; Pharmaceutical Vehicles; Sensitivity and Specificity; Sodium Azide; Sulfhydryl Reagents; Transistors, Electronic; Urea; Urease | 1996 |
Folding and stability of endoglucanase III, a single-domain cellulase from Trichoderma reesei.
Topics: Bacterial Proteins; Cellulase; Circular Dichroism; Disulfides; Dithiothreitol; Enzyme Stability; Hydrogen-Ion Concentration; Kinetics; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Spectrometry, Fluorescence; Temperature; Thermodynamics; Trichoderma; Tryptophan; Urea | 1996 |
Urea/DTT solubilization of a recombinant Taenia ovis antigen, 45W, expressed as a GST fusion protein results in enhanced protective immune response to the 45W moiety.
Topics: Animals; Antibodies, Helminth; Antigens, Helminth; Dithiothreitol; Glutathione Transferase; Linear Models; Male; Recombinant Fusion Proteins; Sheep; Solubility; Taenia; Urea; Vaccines, Synthetic | 1997 |
Evidence for a specific interaction of vitronectin with arginine: effects of reducing agents on the expression of functional domains and immunoepitopes.
Topics: Antibodies, Monoclonal; Arginine; Binding Sites, Antibody; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Epitopes; Hot Temperature; Humans; Plasminogen Activator Inhibitor 1; Protein Conformation; Protein Denaturation; Reducing Agents; Somatomedins; Urea; Vitronectin | 1997 |
The RNA-N-glycosidase activity of Shiga-like toxin I: kinetic parameters of the native and activated toxin.
Topics: Animals; Bacterial Toxins; Dithiothreitol; Kinetics; N-Glycosyl Hydrolases; Rabbits; Ribosome Inactivating Proteins; Shiga Toxin 1; Trypsin; Urea | 1997 |
Semenogelin I and semenogelin II, the major gel-forming proteins in human semen, are substrates for transglutaminase.
Topics: Cadaverine; Calcium; Cross-Linking Reagents; Disulfides; Dithiothreitol; Fluorescent Dyes; Gonadal Steroid Hormones; Humans; Male; Peptide Fragments; Prostate-Specific Antigen; Recombinant Proteins; Semen; Seminal Vesicle Secretory Proteins; Solubility; Substrate Specificity; Transglutaminases; Urea | 1998 |
Carbamylation of cysteine: a potential artifact in peptide mapping of hemoglobins in the presence of urea.
Topics: Alkylation; Amino Acid Sequence; Cysteine; Dithiothreitol; Gas Chromatography-Mass Spectrometry; Hemoglobins; Humans; Hydrogen-Ion Concentration; Hydroxylamine; Peptide Mapping; Recombinant Proteins; Serine Endopeptidases; Urea | 1999 |
Chemical treatment of Escherichia coli: 3. Selective extraction of a recombinant protein from cytoplasmic inclusion bodies in intact cells.
Topics: Bioreactors; Biotechnology; Cell Membrane Permeability; Disulfides; Dithiothreitol; Edetic Acid; Escherichia coli; Ethanol; Inclusion Bodies; Indicators and Reagents; Insulin-Like Growth Factor I; Recombinant Proteins; Solubility; Urea | 1999 |
Chemical treatment of Escherichia coli. II. Direct extraction of recombinant protein from cytoplasmic inclusion bodies in intact cells.
Topics: Biochemistry; Cell Membrane Permeability; Dithiothreitol; Edetic Acid; Escherichia coli; Hydrogen-Ion Concentration; Inclusion Bodies; Insulin-Like Growth Factor I; Kinetics; Recombinant Proteins; Urea | 1998 |
Oxidative refolding of recombinant prochymosin.
Topics: Air; Chymosin; Circular Dichroism; Cyanogen Bromide; Cysteine; Disulfides; Dithiothreitol; Enzyme Precursors; Escherichia coli; Glutathione; Hydrogen-Ion Concentration; Inclusion Bodies; Models, Chemical; Oxidation-Reduction; Peptide Fragments; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Solubility; Spectrometry, Fluorescence; Sulfhydryl Compounds; Urea | 1999 |
Assignment of a single disulfide bridge in rat liver methionine adenosyltransferase.
Topics: Amino Acid Sequence; Animals; Cellulase; Cysteine; Dimerization; Disulfides; Dithiothreitol; Ethylmaleimide; Liver; Methionine Adenosyltransferase; Molecular Sequence Data; Nitric Oxide Donors; Peptide Fragments; Peptide Mapping; Protein Denaturation; Protein Structure, Quaternary; Rats; Sequence Analysis, Protein; Trypsin; Urea | 2000 |
The ecto-5'-nucleotidase subunits in dimers are not linked by disulfide bridges but by non-covalent bonds.
Topics: 5'-Nucleotidase; Animals; Cattle; Centrifugation, Density Gradient; Chromatography, High Pressure Liquid; Dimerization; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Guanidine; Male; Protein Structure, Quaternary; Sulfhydryl Compounds; Urea | 2000 |
Suitable experimental conditions are required to characterize interferon-alpha2b synthetic peptides.
Topics: Binding Sites; Cell Division; Cell Line; Cell Membrane; Chromatography, Gel; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Epitopes; Humans; Interferon alpha-2; Interferon-alpha; Mercaptoethanol; Peptides; Protein Binding; Recombinant Proteins; Urea | 2000 |
Reactivation and refolding of reassociated dimers of rabbit muscle creatine kinase.
Topics: Animals; Creatine Kinase; Dimerization; Dithionitrobenzoic Acid; Dithiothreitol; Kinetics; Models, Biological; Muscles; Protein Denaturation; Protein Folding; Protein Structure, Quaternary; Rabbits; Spectrometry, Fluorescence; Sulfhydryl Reagents; Urea | 2000 |
The native metastable fold of C1-inhibitor is stabilized by disulfide bonds.
Topics: alpha 1-Antitrypsin; Binding Sites; Complement C1 Inactivator Proteins; Complement C1 Inhibitor Protein; Cysteine; Cysteine Proteinase Inhibitors; Disulfides; Dithiothreitol; Hot Temperature; Humans; Oxidation-Reduction; Protein Conformation; Protein Folding; Protein Structure, Secondary; Urea | 2000 |
The residual pro-part of cathepsin C fulfills the criteria required for an intramolecular chaperone in folding and stabilizing the human proenzyme.
Topics: Cathepsin C; Chromatography, Gel; Circular Dichroism; Colorimetry; Dithiothreitol; Enzyme Precursors; Enzyme Stability; Guanidine; Humans; Kinetics; Molecular Chaperones; Oxidation-Reduction; Peptide Fragments; Protein Binding; Protein Denaturation; Protein Folding; Protein Renaturation; Recombinant Proteins; Reducing Agents; Thermodynamics; Urea | 2000 |
Effect of denaturants on the emulsifying activity of proteins.
Topics: Apoproteins; Caseins; Dithiothreitol; Emulsions; Lactoglobulins; Muramidase; Myoglobin; Protein Denaturation; Proteins; Serum Albumin, Bovine; Urea | 2001 |
Fibronectin in the vitreous body--distribution and possible functional role.
Topics: Animals; Cattle; Chondroitin ABC Lyase; Collagen; Disaccharides; Dithiothreitol; Fibronectins; Fluorescent Antibody Technique, Indirect; Glycosaminoglycans; Immunohistochemistry; Microscopy, Immunoelectron; Potassium Chloride; Tissue Distribution; Trypsin; Urea; Uronic Acids; Vitreous Body | 2001 |
Detection of disulfide bonds in bovine brain tubulin and their role in protein folding and microtubule assembly in vitro: a novel disulfide detection approach.
Topics: Ammonium Sulfate; Animals; Brain Chemistry; Cattle; Colchicine; Cysteine; Disulfides; Dithiothreitol; Microtubules; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Reducing Agents; Spectrometry, Fluorescence; Tryptophan; Tubulin; Urea | 2001 |
Influence of a sulfhydryl cross-link across the allosteric-site interface of E. coli phosphofructokinase.
Topics: Allosteric Regulation; Allosteric Site; Binding Sites; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Enzyme Stability; Escherichia coli; Phosphofructokinase-1; Pressure; Protein Folding; Sulfhydryl Reagents; Urea | 2001 |
Yield and post-yield behavior of mussel byssal thread: a self-healing biomolecular material.
Topics: Animals; Biopolymers; Bivalvia; Chelating Agents; Collagen; Dithiothreitol; Edetic Acid; Macromolecular Substances; Protein Denaturation; Proteins; Seawater; Urea | 2001 |
Biochemistry and pharmacology of epitope-tagged alpha(1)-adrenergic receptor subtypes.
Topics: Blotting, Western; Calcium; Cells, Cultured; Chromatography, Affinity; Dithiothreitol; Epitopes; Humans; Nickel; Nitrilotriacetic Acid; Photoaffinity Labels; Precipitin Tests; Radioligand Assay; Receptors, Adrenergic, alpha-1; Resins, Plant; Sulfhydryl Reagents; Urea | 2002 |
Role of protein disulfide isomerase in molecular fate of thyroglobulin and its regulation by endogenous oxidants and reductants.
Topics: Animals; Cattle; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Endoplasmic Reticulum; Glutathione; Goiter; Humans; Hydrolysis; Liver; Molecular Conformation; Oxidants; Oxidation-Reduction; Protein Disulfide-Isomerases; Protein Folding; Reducing Agents; Surface-Active Agents; Swine; Thermolysin; Thyroglobulin; Urea | 2002 |
Unfolding and pH studies on manganese peroxidase: role of heme and calcium on secondary structure stability.
Topics: Apoproteins; Calcium; Circular Dichroism; Dithiothreitol; Enzyme Stability; Heme; Holoenzymes; Hydrogen-Ion Concentration; Models, Molecular; Oxidation-Reduction; Peroxidases; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Urea | 2003 |
Subunit dissociation, unfolding, and inactivation of bothrojaracin, a C-type lectin-like protein from snake venom.
Topics: Animals; Antithrombins; Crotalid Venoms; Dimerization; Disulfides; Dithiothreitol; Humans; Lectins, C-Type; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Protein Subunits; Thrombin; Urea | 2003 |
Expression, oxidative refolding, and characterization of six-histidine-tagged recombinant human LECT2, a 16-kDa chemotactic protein with three disulfide bonds.
Topics: Animals; CHO Cells; Circular Dichroism; Cricetinae; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Humans; Intercellular Signaling Peptides and Proteins; Magnetic Resonance Spectroscopy; Oxidation-Reduction; Oxygen; Protein Binding; Protein Conformation; Protein Folding; Proteins; Recombinant Proteins; Sepharose; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrophotometry; Time Factors; Urea | 2003 |
Enhanced resolution of glycosylphosphatidylinositol-anchored and transmembrane proteins from the lipid-rich myelin membrane by two-dimensional gel electrophoresis.
Topics: Acetone; Animals; Blotting, Western; Cell Membrane; Cholic Acids; Detergents; Dithiothreitol; Edetic Acid; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Polyacrylamide Gel; Ethanol; Glycosylphosphatidylinositols; Lipids; Mice; Mice, Inbred C57BL; Myelin Sheath; Proteins; Silver Staining; Thiourea; Urea | 2003 |
Polyacrylamide gel electrophoresis followed by sodium dodecyl sulfate gradient polyacrylamide gel electrophoresis for the study of the dimer to monomer transition of human transthyretin.
Topics: Amyloid; Dimerization; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Humans; Hydrogen-Ion Concentration; Prealbumin; Temperature; Titrimetry; Urea | 2003 |
On-chip protein sample desalting and preparation for direct coupling with electrospray ionization mass spectrometry.
Topics: Adsorption; Cysteine; Cytochromes c; Dithiothreitol; Indicators and Reagents; Insulin; Iodoacetamide; Lactoglobulins; Propranolol; Protein Array Analysis; Proteins; Salts; Spectrometry, Mass, Electrospray Ionization; Urea | 2003 |
Solid-phase reducing agents as alternative for reducing disulfide bonds in proteins.
Topics: Aspergillus oryzae; beta-Galactosidase; Chromatography, Gel; Disulfides; Dithiothreitol; Hydrogen-Ion Concentration; Kinetics; Kluyveromyces; Lactoglobulins; Models, Chemical; Sepharose; Sulfhydryl Compounds; Temperature; Urea | 2003 |
Cross-linking approach to affinity capture of protein complexes from chaotrope-solubilized cell lysates.
Topics: Affinity Labels; Calreticulin; Cell Line; Cross-Linking Reagents; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Histidine; Humans; Interleukin-12; Nickel; Nitrilotriacetic Acid; Peptides; Plasmids; Protein Denaturation; Proteins; Succinimides; Sulfhydryl Compounds; Urea | 2004 |
The role of disulfide bonds in the conformational stability and catalytic activity of phytase.
Topics: 6-Phytase; Catalysis; Circular Dichroism; Disulfides; Dithiothreitol; Protein Conformation; Protein Denaturation; Urea | 2004 |
Intramolecular disulfide bonds are required for folding hydrolase B into a catalytically active conformation but not for maintaining it during catalysis.
Topics: Animals; Carboxylesterase; Carboxylic Ester Hydrolases; Cysteine; Disulfides; Dithiothreitol; Microsomes, Liver; Mutagenesis, Site-Directed; Oxidation-Reduction; Protein Conformation; Protein Denaturation; Protein Folding; Rats; Urea | 2004 |
Confirmation of Vpr as a fibrinolytic enzyme present in extracellular proteins of Bacillus subtilis.
Topics: Bacillus subtilis; Bacterial Proteins; Dithiothreitol; Electrophoresis, Gel, Two-Dimensional; Serine Endopeptidases; Urea | 2005 |
Comparative study of enzyme activity and stability of bovine and human plasmins in electrophoretic reagents, beta-mercaptoethanol, DTT, SDS, Triton X-100, and urea.
Topics: Animals; Cattle; Detergents; Dithiothreitol; Electrophoresis; Enzyme Stability; Fibrinolysin; Humans; Indicators and Reagents; Mercaptoethanol; Octoxynol; Protein Denaturation; Reducing Agents; Sodium Dodecyl Sulfate; Urea | 2005 |
Influence of disulfide bonds on the conformational changes and activities of refolded phytase.
Topics: 6-Phytase; Buffers; Circular Dichroism; Disulfides; Dithiothreitol; Enzyme Activation; Hydrogen-Ion Concentration; Protein Denaturation; Protein Folding; Protein Renaturation; Solutions; Spectrometry, Fluorescence; Urea | 2005 |
Comparison of extraction procedures for proteome analysis of Streptococcus pneumoniae and a basic reference map.
Topics: Amino Acid Sequence; Antigens, Bacterial; Bacterial Proteins; Cholic Acids; Dithiothreitol; Electrophoresis, Gel, Two-Dimensional; Molecular Sequence Data; Proteome; Sodium Dodecyl Sulfate; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Streptococcus pneumoniae; Urea | 2006 |
Conformational stability of secretory leucocyte protease inhibitor: a protein with no hydrophobic core and very little secondary structure.
Topics: Chromatography, High Pressure Liquid; Cysteine; Cystine; Dithiothreitol; Guanidine; Guanidines; Humans; Hydrophobic and Hydrophilic Interactions; Isothiocyanates; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Proteinase Inhibitory Proteins, Secretory; Proteins; Recombinant Proteins; Secretory Leukocyte Peptidase Inhibitor; Thermodynamics; Urea | 2006 |
Cloning, expression and characterization of a thermostable exo-beta-D-glucosaminidase from the hyperthermophilic archaeon Pyrococcus horikoshii.
Topics: Cations, Divalent; Cloning, Molecular; Dimerization; Dimethyl Sulfoxide; Dithiothreitol; Enzyme Stability; Escherichia coli; Guanidine; Hexosaminidases; Hydrogen-Ion Concentration; Pyrococcus horikoshii; Recombinant Proteins; Sodium Dodecyl Sulfate; Temperature; Urea | 2006 |
Urea stimulation of KCl cotransport induces abnormal volume reduction in sickle reticulocytes.
Topics: Anemia, Sickle Cell; Biological Transport; Cell Size; Disulfides; Dithiothreitol; Hemoglobins; Humans; Oxidation-Reduction; Potassium Chloride; Reticulocytes; Urea | 2007 |
Analysis of serine proteases from marine sponges by 2-D zymography.
Topics: Animals; Benzoylarginine Nitroanilide; Dithiothreitol; Electrophoresis, Gel, Two-Dimensional; Enzyme Activation; Mercaptoethanol; Molecular Weight; Porifera; Serine Endopeptidases; Sodium Dodecyl Sulfate; Substrate Specificity; Urea | 2007 |
Refolding of partially and fully denatured lysozymes.
Topics: Animals; Chickens; Dithiothreitol; Glutathione Disulfide; Muramidase; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Urea | 2007 |
Quaternary structure and apical membrane sorting of the mammalian NaSi-1 sulfate transporter in renal cell lines.
Topics: Animals; Anticholesteremic Agents; Brefeldin A; Cation Transport Proteins; Cell Line; Cell Membrane; Detergents; Dimerization; Dithiothreitol; Dogs; Electrophoresis, Polyacrylamide Gel; Female; Glycosylation; Golgi Apparatus; Kidney; Membrane Microdomains; Oocytes; Opossums; Protein Transport; Rats; Sodium Sulfate Cotransporter; Swine; Symporters; Tunicamycin; Urea; Xenopus laevis | 2007 |
Effect of phosphorylation on alpha B-crystallin: differences in stability, subunit exchange and chaperone activity of homo and mixed oligomers of alpha B-crystallin and its phosphorylation-mimicking mutant.
Topics: alpha-Crystallin B Chain; alpha-Synuclein; Amino Acid Substitution; Amyloid; Aspartic Acid; Citrate (si)-Synthase; Dimerization; Dithiothreitol; Dose-Response Relationship, Drug; Heat-Shock Proteins, Small; Hot Temperature; Humans; Insulin; Molecular Chaperones; Phosphorylation; Protein Denaturation; Protein Structure, Quaternary; Protein Structure, Secondary; Protein Structure, Tertiary; Protein Subunits; Recombinant Proteins; Sodium Dodecyl Sulfate; Surface-Active Agents; Urea | 2008 |
Purification and characterization of a novel beta-agarase, AgaA34, from Agarivorans albus YKW-34.
Topics: Alteromonadaceae; Animals; Chromatography, Gel; Chromatography, Ion Exchange; Disaccharides; Dithiothreitol; DNA, Bacterial; Enzyme Activators; Enzyme Inhibitors; Enzyme Stability; Galactosides; Gastropoda; Glycoside Hydrolases; Hydrogen-Ion Concentration; Kinetics; Magnetic Resonance Spectroscopy; Mercaptoethanol; Molecular Sequence Data; Molecular Weight; Oligosaccharides; Sepharose; Sequence Analysis, DNA; Sequence Analysis, Protein; Sequence Homology, Amino Acid; Sodium Dodecyl Sulfate; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Temperature; Urea | 2008 |
One-dimensional SDS gel electrophoresis of proteins.
Topics: Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Gels; Glycine; Mercaptoethanol; Molecular Weight; Peptides; Protein Denaturation; Proteins; Sodium Dodecyl Sulfate; Tromethamine; Urea | 2006 |
Use of protein folding reagents.
Topics: Dithiothreitol; Glutathione; Guanidine; Mercaptoethanol; Oxidants; Protein Denaturation; Protein Folding; Reducing Agents; Sulfhydryl Reagents; Urea | 2001 |
Evaluating the compatibility of three colorimetric protein assays for two-dimensional electrophoresis experiments.
Topics: Animals; Cattle; Cell Line; Colorimetry; Dithiothreitol; Electrophoresis, Gel, Two-Dimensional; Humans; Proteins; Proteomics; Quinolines; Reproducibility of Results; Serum Albumin, Bovine; Urea | 2008 |
Enrichment of serum low-molecular-weight proteins using C18 absorbent under urea/dithiothreitol denatured environment.
Topics: Blood Proteins; Cell Line; Dithiothreitol; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Polyacrylamide Gel; Humans; Immunoglobulin G; Molecular Weight; Protein Denaturation; Reproducibility of Results; Resins, Synthetic; Serum Albumin; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Urea | 2010 |
Acute effects of diesel emission from the urea selective catalytic reduction engine system on male rats.
Topics: 8-Hydroxy-2'-Deoxyguanosine; Air Pollutants, Occupational; Air Pollution; Animals; Bronchoalveolar Lavage Fluid; Catalysis; Deoxyguanosine; Dithiothreitol; Lung; Male; Nitrogen Dioxide; Oligonucleotide Array Sequence Analysis; Organ Size; Oxidants, Photochemical; Oxidative Stress; Particulate Matter; Rats; Rats, Inbred F344; RNA, Messenger; Sulfhydryl Reagents; Trachea; Urea; Vehicle Emissions; Weight Gain | 2010 |
Effects of dithiothreitol on the amyloid fibrillogenesis of hen egg-white lysozyme.
Topics: Amyloid; Animals; Avian Proteins; Central Nervous System Agents; Chickens; Dithiothreitol; Egg Proteins; Egg White; Female; Hydrophobic and Hydrophilic Interactions; Kinetics; Muramidase; Oxidation-Reduction; Protein Conformation; Protein Folding; Protein Multimerization; Protein Stability; Thermodynamics; Urea | 2010 |
Characterisation of low abundance wool proteins through novel differential extraction techniques.
Topics: Amino Acid Sequence; Animals; Dithiothreitol; Electrophoresis; Electrophoresis, Gel, Two-Dimensional; Hair; Hydrogen-Ion Concentration; Keratins, Hair-Specific; Molecular Sequence Data; Molecular Weight; Peptides; Sheep; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Urea; Wool | 2010 |
Protein extraction and two-dimensional gel electrophoresis of proteins in the marine mussel Mytilus galloprovincialis: an important tool for protein expression studies, food quality and safety assessment.
Topics: Animals; Biomarkers; Buffers; Cholic Acids; Cytoskeleton; Diet; Dithiothreitol; Electrophoresis, Gel, Two-Dimensional; Food Contamination; Food Safety; Gills; Humans; Hydrogen-Ion Concentration; Mytilus; Proteome; Proteomics; Shellfish; Thiourea; Urea | 2013 |
Electrophoretic separation of purified myelin: a method to improve the protein pattern resolving.
Topics: Animals; Blotting, Western; Cattle; Cholic Acids; Detergents; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Glucosides; Myelin Proteins; Myelin Sheath; Protein Denaturation; Reducing Agents; Urea | 2013 |
Manipulating hepatocellular carcinoma cell fate in orthogonally cross-linked hydrogels.
Topics: Carcinoma, Hepatocellular; Cell Dedifferentiation; Cell Line, Tumor; Cell Survival; Cells, Immobilized; Click Chemistry; Cytochrome P-450 CYP3A; Dithiothreitol; Fibronectins; Hep G2 Cells; Hepatocytes; Humans; Hydrogels; Liver Neoplasms; Matrix Metalloproteinases; Norbornanes; Organic Anion Transporters, Sodium-Dependent; Polyethylene Glycols; RNA, Messenger; Symporters; Tissue Culture Techniques; Up-Regulation; Urea | 2014 |
Impact of extraction and elution media on non-size effects in size exclusion chromatography of proteins.
Topics: Acetonitriles; Animals; Cattle; Chromatography, Gel; Dithiothreitol; Globulins; Glutens; Molecular Weight; Ovalbumin; Plant Proteins; Serum Albumin, Bovine; Sodium Chloride; Sodium Dodecyl Sulfate; Solvents; Soybean Proteins; Trifluoroacetic Acid; Triticum; Urea | 2015 |