urea has been researched along with alpha-chymotrypsin in 189 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 150 (79.37) | 18.7374 |
| 1990's | 21 (11.11) | 18.2507 |
| 2000's | 6 (3.17) | 29.6817 |
| 2010's | 12 (6.35) | 24.3611 |
| 2020's | 0 (0.00) | 2.80 |
| Authors | Studies |
|---|---|
| Belew, M; Porath, J; Sundberg, L | 1 |
| Ishii, S; Kasai, K | 1 |
| Korsgaard, B; Pedersen, JT | 1 |
| Gabel, D; Kasche, V | 1 |
| Constantinides, SM; Marangos, PJ | 1 |
| Singer, DS; Singer, MF | 1 |
| Creighton, TE | 2 |
| Pace, CN | 1 |
| Osawa, T; Sawada, JI; Shioiri-Nakano, K | 1 |
| Mizel, SB | 1 |
| Ebina, S; Kobayashi, T; Nagai, Y | 1 |
| Fanick, W; Hilz, H | 1 |
| Hibbard, LS; Tulinsky, A | 1 |
| Fowler, AV; Langley, KE; Zabin, I | 1 |
| Chatagner, F; de Billy, G; Muller, P | 1 |
| Berezin, IV; Goldmacher, VS; Klibanov, AM; Martinek, K | 1 |
| Alfsen, A; de Foresta, B; Lavialle, F | 1 |
| Chen, LH; Foster, JA; Occhino, JC; Schmid, K; Sperandio, K | 1 |
| Burlini, N; Guerritore, A; Macchi, F; Magnani, P; Tortora, P; Villa, A | 1 |
| Kaur, J; Tnakur, MK | 1 |
| Laine, RA; Zhu, BC | 2 |
| Peberdy, JF; Vainstein, MH | 1 |
| Emde, C; Herrmann, ME; Hopert, R; Liehr, RM; Riecken, EO | 1 |
| Brown, ML; Feuer, DS; Hesler, CB; Marcel, YL; Milne, RW; Tall, AR | 1 |
| Kamiya, S; Konno, K; Watanabe, S; Yoshitomi, B | 1 |
| Frank, P; Rahn, R | 1 |
| Bolen, DW; Santoro, MM | 1 |
| Ishii, S; Kumazaki, T; Terasawa, K | 1 |
| Bunce, JD; Venanzi, CA | 1 |
| Allen, RG; Barrett, JT | 1 |
| Kopec, M; Latallo, ZS; Teisseyre, E; Wegrzynowicz, Z | 1 |
| Becker, U; Kühn, K; Timpl, R | 1 |
| Datyner, A; Finnimore, ED | 1 |
| Kapitany, RA; Zebrowski, EJ | 1 |
| Kleinschmidt, AK; Vásquez, C | 1 |
| Thiele, HG | 1 |
| Bushuk, W; Kaminski, E | 1 |
| Bauer, CA; Löfqvist, B | 1 |
| Linden, G; Nicolas, JP; Wolff, R | 1 |
| Madapallimattam, G; Saidel, LJ | 1 |
| Crowell, RL; Zajac, I | 1 |
| Berliner, LJ | 1 |
| Karstad, L; Tsai, KS | 1 |
| Heusser, C; Isliker, H; Knobel, HR; Rodrick, ML | 1 |
| Cabral, F; Love, B | 1 |
| Deutsch, HF; Fett, JW | 1 |
| Greene, RF; Pace, CN | 1 |
| Crozier, N; Lloyd, EA; Pamphlet, G; Saunders, SJ; Wells, M | 1 |
| Iinuma, F; Kinoshita, T; Tsuji, A | 3 |
| Gaudin, J; Stevens, FC | 1 |
| Braunshtein, AE; Deev, SM; Grishin, EV; Nosikov, VV; Polyanovskii, OL | 1 |
| Hyde, JE; Walker, IO | 1 |
| Ivanov, VI; Makarov, AA; Vol'kenshtein, MV | 1 |
| Ui, N | 1 |
| Benz, FW; Roberts, GC | 1 |
| Ristow, SS; Wetlaufer, DB | 1 |
| Bielański, W; Luchter-Wasyl, E; Wasyl, Z | 1 |
| Chibata, I; Mori, T; Tosa, T | 1 |
| Canfield, RE; Goodman, DS; Kanda, Y; Morgan, FJ | 1 |
| Melville, JC; Ryan, CA | 1 |
| Conard, RA; O, YH | 1 |
| Kühn, K; Stolz, M; Timpl, R | 1 |
| Cotman, CW; Taylor, D | 1 |
| Bing, DH; Mernitz, JL; Spurlock, SE | 1 |
| Forman, HJ; Fridovich, I | 1 |
| Furthmayr, H; Stoltz, M; Timpl, R | 1 |
| Hopkins, TR; Spikes, JD | 2 |
| Avery, MJ; Hopkins, TR | 1 |
| Busse, WD; Gattner, HG | 1 |
| Antonov, VK; Chekhlyaeva, NM; Kogan, GA; Kozlov, LV; Slobodyanskaya, EM | 1 |
| Ananthanarayanan, VS; Bigelow, CC | 1 |
| Kato, T; Miall, SH; Tamaoki, T | 1 |
| Giroux, EL; Henkin, RI | 1 |
| Nakagawa, Y; Perlmann, GE | 1 |
| Matsuda, G; Shiozawa, T; Takei, H; Wu, KC | 2 |
| Allen, PZ; Sirisinha, S | 1 |
| Calissano, P; Friesen, A; Moore, BW | 1 |
| Inada, M | 1 |
| Horikoshi, K | 1 |
| Boucher, ME; Bromer, WW; Frank, BH; Patterson, JM; Pekar, AH | 1 |
| Gaudin, JE; Viswanatha, T | 1 |
| Brown, JC; Horton, HR; Swaisgood, HE | 1 |
| Derouette, JC; Robert, B; Robert, L; Szigeti, M | 1 |
| Heinrikson, RL | 1 |
| Fujiwara, N; Maekawa, T; Matsuda, G | 1 |
| Cacace, MG; Dosseva, I; Pierro, M; Rossi, M; Scarano, E | 1 |
| Stanton, M; Viswanatha, T | 1 |
| Gabel, D; Katchalski, E; Steinberg, IZ | 1 |
| Gersheovich, ZS; Krichevskaia, AA; Lukash, AI | 1 |
| Liener, IE; Seidl, DS | 1 |
| Lawrence, TG; WIilliams, RC | 1 |
| Blout, ER; Sigman, DS; Torchia, DA | 1 |
| Ficklin, WH; Gibian, MJ; Rubenstein, PA | 1 |
| Yogo, I | 1 |
| Corfield, MC; Fletcher, JC | 1 |
| Glick, DM; Olm, LM | 1 |
| Gundlach, HG | 1 |
| Deutsch, HF; Miekka, SI | 1 |
| Dolecek, V; Lapanje, S; Skerjanc, J | 1 |
| Garner, CW; Smith, LC | 1 |
| DeSena, T; Herskovits, TT; Jaillet, H | 1 |
| Castañeda-Agulló, M; Del Castillo, LM | 1 |
| Akazawa, T; Nakayama, N; Ogawa, M; Sugiyama, T | 1 |
| Tagaki, W; Westheimer, FH | 1 |
| Abita, JP; Delaage, M; Lazdunski, M | 1 |
| Miyamoto, U | 1 |
| Bailey, JE; Beaven, GH; Chignell, DA; Gratzer, WB | 1 |
| Hofstee, BH | 1 |
| Ando, T; Takami, T | 1 |
| Brecher, AS; Friedberg, F; Long, JE | 1 |
| Abita, JP; Lazdunski, M | 1 |
| Maddaiah, VT | 1 |
| Elfring, WH; Leitzes, S; Saidel, LJ | 1 |
| Glazer, AN; Sanger, F | 1 |
| Duesberg, PH; Rueckert, RR | 1 |
| Raval, DN; Schellman, JA | 1 |
| Mosolov, VV | 1 |
| Gombos, G; Kornblueth, W; Traub, B | 1 |
| Bhatnagar, GM; Martin, CJ | 2 |
| Richmond, V | 1 |
| Stellwagen, E | 1 |
| Schade, W; Zahn, H; Ziegler, K | 1 |
| Mandel, B | 1 |
| Baintner, K; Sommer, A | 1 |
| Fudenberg, HH; Tung, E; Wang, AC | 1 |
| Kortt, AA | 1 |
| Engel, J; Hörmann, H; Marković, Z; Richter, H | 1 |
| Ebina, S; Nagai, Y | 1 |
| Gorovits, BM; Horowitz, PM | 1 |
| Gorovits, BM; Horowitz, PM; Seale, JW | 1 |
| Bernhardt, R; Burova, TV; Pfeil, W | 1 |
| Gibbons, DL; Horowitz, PM; Hua, S | 1 |
| Bhattacharyya, B; Sackett, DL; Wolff, J | 1 |
| Cawood, PG; Erickson-Viitanen, S; Klabe, RM; Meek, JL; O'Neal, PL | 1 |
| Jaenicke, R; Lehle, K; Wrba, A | 1 |
| Saeed, T; Salahuddin, A | 1 |
| Mozhaev, VV; Panova, AA | 1 |
| Miyauchi, M; Tozawa, K; Yoshida, M | 1 |
| Bolen, DW; Huang, Y | 1 |
| Cornell, RB; Veitch, DP | 1 |
| Kriwacki, RW; Siuzdak, G; Tennant, L; Wright, PE; Wu, J | 1 |
| Feng, S; Woo, PT | 1 |
| Sundaram, PV; Venkatesh, R | 1 |
| Devaraj, VR; Manjunatha, NH | 1 |
| Colombo, R; DalleDonne, I; Milzani, A | 1 |
| Clark, DS; Guo, Y | 1 |
| Chang, JY; Lu, BY | 1 |
| HARRIS, JI | 1 |
| DREYER, WJ; NEURATH, H; RUPLEY, JA | 1 |
| CHERVENKA, CH | 1 |
| KONDO, M; MIKI, N; NAITO, M | 1 |
| FRAZIER, AR; MARTIN, CJ | 2 |
| HOFSCHNEIDER, PH; MUELLER-JENSEN, K | 1 |
| BISERTE, G; CUVELIER, R; HAVEZ, R | 1 |
| MEE, LK | 1 |
| DIXON, GH; SCHACHTER, H | 1 |
| RIESER, CH; RIESER, P | 1 |
| RIGBI, M; SELA, M | 1 |
| FAHRNEY, D; GOLD, AM | 1 |
| SORKIN, E | 1 |
| RINIKER, B | 1 |
| HAMMES, GG | 1 |
| MARTIN, CJ | 2 |
| ADAMS-MAYNE, M; WARD, DN | 1 |
| Bouvier, ES; Gebler, JC; Gilar, M; Lee, PJ; Yu, YQ | 1 |
| Baskakov, IV; Gryczynski, Z; Legname, G; Prusiner, SB | 1 |
| Gupta, MN; Roy, I | 1 |
| Lee, MJ; Lin, HM; Venkatesu, P | 1 |
| Attri, P; Kumar, A; Venkatesu, P | 1 |
| Aschi, A; Elmissaoui, S; Ghaouar, N; Gharbi, A | 1 |
| Negoda, A; Negoda, E; Reusch, RN | 1 |
| Attri, P; Byrne, N; Kumar, A; Venkatesu, P | 1 |
| Congyu, K; Qunzheng, Z; Wujuan, S; Xindu, G | 1 |
| Minton, AP; Wu, D | 1 |
| Carmeli, S; Elkobi-Peer, S | 1 |
| Luong, TQ; Winter, R | 1 |
| Garringer, M; Qasim, MA | 1 |
| Canales, R; Held, C; Luong, TQ; Sadowski, G; Verevkin, SP; Wangler, A; Winter, R; Zaitsau, DH | 1 |
| Anand, GS; Lim, XX; Raghuvamsi, PV; Ramesh, R; Wong, SM; Wu, C | 1 |
| Held, C; Knierbein, M; Luong, TQ; Sadowski, G; Wangler, A; Winter, R | 1 |
1 review(s) available for urea and alpha-chymotrypsin
| Article | Year |
|---|---|
The stability of globular proteins.
Topics: Animals; Cattle; Chymotrypsin; Cytochrome c Group; Female; Guanidines; Humans; Hydrogen Bonding; Hydrogen-Ion Concentration; Immunoglobulin Fragments; Lactoglobulins; Models, Chemical; Muramidase; Myoglobin; Osmolar Concentration; Phosphorylases; Protein Conformation; Protein Denaturation; Ribonucleases; Statistics as Topic; Temperature; Thermodynamics; Urea | 1975 |
188 other study(ies) available for urea and alpha-chymotrypsin
| Article | Year |
|---|---|
The trypsin and chymotrypsin inhibitors in chick peas (Cicer arietinum L.). Purification and properties of the inhibitors.
Topics: Amino Acids; Chromatography, Affinity; Chymotrypsin; Drug Stability; Guanidines; Hydrogen-Ion Concentration; Molecular Weight; Pepsin A; Plant Proteins; Plants; Temperature; Trypsin Inhibitors; Urea | 1975 |
Affinity chromatography of trypsin and related enzymes. I. Preparation and characteristics of an affinity adsorbent containing tryptic peptides from protamine as ligands.
Topics: Amino Acids; Benzamidines; Chromatography, Affinity; Chymotrypsin; Hydrogen-Ion Concentration; Ligands; Oligopeptides; Osmolar Concentration; Protamines; Sepharose; Streptomyces griseus; Temperature; Trypsin; Trypsinogen; Urea | 1975 |
[Sputum and expectorants].
Topics: Acetylcysteine; Ammonium Chloride; Antimony; Bromhexine; Chymotrypsin; Expectorants; Humans; Iodides; Ipecac; Lauric Acids; Polysorbates; Saponins; Sputum; Urea; Water | 1976 |
Conformational transitions in immobilized proteases.
Topics: Chymotrypsin; Enzymes, Immobilized; Gels; Hydrogen-Ion Concentration; Kinetics; Methods; Protein Binding; Protein Conformation; Temperature; Thermodynamics; Trypsin; Urea | 1976 |
Effect of NAD on flounder muscle glyceraldehyde 3-phosphate dehydrogenase.
Topics: Adenosine Triphosphate; Animals; Chymotrypsin; Drug Stability; Fishes; Glyceraldehyde-3-Phosphate Dehydrogenases; Muscles; NAD; Protein Denaturation; Trypsin; Urea | 1975 |
Studies on the interaction of H1 histone with superhelical DNA: characterization of the recognition and binding regions of H1 histones.
Topics: Animals; Bromosuccinimide; Cattle; Chymotrypsin; DNA, Circular; DNA, Viral; Histones; Nucleic Acid Conformation; Osmolar Concentration; Peptides; Protein Binding; Protein Conformation; Simian virus 40; Sodium Chloride; Structure-Activity Relationship; Thymus Gland; Urea | 1976 |
Electrophoretic analysis of the unfolding of proteins by urea.
Topics: Chymotrypsin; Chymotrypsinogen; Cytochrome c Group; Electrophoresis, Polyacrylamide Gel; Lactalbumin; Lactoglobulins; Micrococcal Nuclease; Muramidase; Ovalbumin; Protein Conformation; Protein Denaturation; Ribonucleases; Serum Albumin, Bovine; Urea | 1979 |
Purification and characterization of guinea pig lymphotoxin produced by lymph node cells stimulated by phytohemagglutinin.
Topics: Ammonium Sulfate; Animals; Cell-Free System; Cells, Cultured; Centrifugation, Density Gradient; Chemical Fractionation; Chromatography, Gel; Chromatography, Ion Exchange; Chymotrypsin; Clostridium perfringens; Concanavalin A; Cytotoxicity Tests, Immunologic; Edetic Acid; Guinea Pigs; Hydrogen-Ion Concentration; Iodoacetates; Lectins; Lymph Nodes; Lymphocytes; Lymphotoxin-alpha; Micrococcal Nuclease; Molecular Weight; Neuraminidase; Nitrobenzenes; Serum Albumin, Radio-Iodinated; Trypsin; Urea | 1975 |
Physicochemical characterization of lymphocyte-activating factor (LAF).
Topics: Alkylation; Animals; Binding Sites; Chemical Phenomena; Chemistry; Chymotrypsin; Concanavalin A; Interleukin-2; Lymphocytes; Lymphokines; Mice; Papain; Peptide Hydrolases; Sodium Dodecyl Sulfate; Trypsin; Urea | 1979 |
Inhibition of alpha-chymotrypsin by 2-halogeno-ethanols; comparisons with 2-methyl-ethanols and urea.
Topics: Animals; Cattle; Chymotrypsin; Ethanol; Kinetics; Pancreas; Structure-Activity Relationship; Urea | 1979 |
Divergent denaturation of proteases by urea and dodecylsulfate in the absence of substrate.
Topics: Chymotrypsin; Kinetics; Peptide Hydrolases; Pronase; Protein Denaturation; Serum Albumin, Bovine; Sodium Dodecyl Sulfate; Subtilisins; Trypsin; Urea | 1978 |
Expression of functionality of alpha-chymotrypsin. Effects of guanidine hydrochloride and urea in the onset of denaturation.
Topics: Chymotrypsin; Guanidines; Protein Binding; Protein Conformation; Protein Denaturation; Urea; X-Ray Diffraction | 1978 |
Amino acid sequence of beta-galactosidase. IV. Sequence of an alpha-complementing cyanogen bromide peptide, residues 3 to 92.
Topics: Amino Acid Sequence; Amino Acids; Chromatography, Gel; Chromatography, Ion Exchange; Chymotrypsin; Cyanogen Bromide; Electrophoresis, Disc; Escherichia coli; Galactosidases; Genetic Complementation Test; Mutation; Peptide Fragments; Trypsin; Urea | 1975 |
New insights into the active center of rat liver cystathionase.
Topics: Animals; Binding Sites; Binding, Competitive; Chymotrypsin; Cystathionine; Cystathionine gamma-Lyase; Homoserine; Kinetics; Liver; Lyases; Protein Conformation; Rats; Structure-Activity Relationship; Urea | 1975 |
Denaturing agents (urea, acrylamide) protect enzymes against irreversible thermoinactivation: a study with native and immobilized alpha-chymotrypsin and trypsin.
Topics: Acrylamides; Animals; Binding Sites; Cattle; Chymotrypsin; Drug Stability; Kinetics; Protein Binding; Protein Denaturation; Solubility; Time Factors; Trypsin; Urea | 1975 |
Thermal and solvent perturbation as probes of conformational heterogeneity of haptoglobin 1-1.
Topics: Animals; Binding Sites; Chymotrypsin; Guanidines; Haptoglobins; Macromolecular Substances; Protein Binding; Protein Conformation; Solvents; Spectrophotometry, Ultraviolet; Temperature; Urea | 1975 |
Topography of human plasma alpha1-acid glycoprotein.
Topics: Amino Acid Sequence; Amino Acids; Chymotrypsin; Circular Dichroism; Glycoproteins; Humans; Nitrophenols; Peptide Fragments; Protein Conformation; Urea | 1976 |
A heat-stable serine proteinase from the extreme thermophilic archaebacterium Sulfolobus solfataricus.
Topics: Amino Acid Sequence; Amino Acids; Chymotrypsin; Enzyme Activation; Hot Temperature; Manganese; Molecular Sequence Data; Molecular Weight; Phenylmethylsulfonyl Fluoride; Serine Endopeptidases; Substrate Specificity; Sulfolobus; Urea | 1992 |
Analysis in vitro of uterine estrogen receptor conformation of young and old rats.
Topics: Adenosine Triphosphate; Aging; Animals; Chymotrypsin; DNA; Electrophoresis, Polyacrylamide Gel; Estradiol; Female; Protein Conformation; Rats; Receptors, Estrogen; Ribonucleases; Tamoxifen; Trypsin; Urea; Uterus | 1991 |
Novel hyperglycosylated weak gelatin-binding fibronectin from human fetal placenta. Fractionation of a high poly(N-acetyllactosamine) fragment by tomato lectin affinity chromatography.
Topics: Adult; beta-Galactosidase; Carbohydrates; Carrier Proteins; Chemical Fractionation; Chromatography, Affinity; Chymotrypsin; Female; Fibronectins; Gas Chromatography-Mass Spectrometry; Gelatin; Glycation End Products, Advanced; Humans; Lectins; Peptide Fragments; Placenta; Polysaccharides; Pregnancy; Urea | 1990 |
Solubilisation of a cell wall bound invertase in Aspergillus nidulans.
Topics: Aspergillus nidulans; beta-Fructofuranosidase; Cell Wall; Chymotrypsin; Dithiothreitol; Endopeptidase K; Ethanol; Glycoside Hydrolases; Octoxynol; Polyethylene Glycols; Polysorbates; Serine Endopeptidases; Sodium Dodecyl Sulfate; Urea | 1990 |
High amount of dietary carbohydrate does not cause an adaptational increase of stimulated human pancreatic amylase secretion.
Topics: Acclimatization; Adult; Amylases; Bicarbonates; Cholesterol; Chymotrypsin; Dietary Carbohydrates; Enteral Nutrition; Female; Humans; Male; Nutritional Status; Pancreas; Prealbumin; Reference Values; Trypsin; Urea | 1989 |
Structure-function analysis of plasma cholesteryl ester transfer protein by protease digestion and expression of cDNA fragments in Escherichia coli.
Topics: Antibodies, Monoclonal; Blotting, Western; Carrier Proteins; Cholesterol Ester Transfer Proteins; Chromatography, Gel; Chymotrypsin; Cloning, Molecular; Dithiothreitol; DNA; Electrophoresis, Polyacrylamide Gel; Epitopes; Escherichia coli; Glycoproteins; Humans; Macromolecular Substances; Molecular Weight; Peptide Fragments; Peptide Hydrolases; Protein Conformation; Serine Endopeptidases; Structure-Activity Relationship; Trypsin; Urea | 1989 |
Heavy meromyosin and subfragment-1 from squid mantle myosin, and Ca-sensitivity of their Mg-ATPases.
Topics: Actins; Actomyosin; Adenosine Triphosphatases; Animals; Ca(2+) Mg(2+)-ATPase; Calcium; Chymotrypsin; Decapodiformes; Enzyme Activation; Macromolecular Substances; Molecular Weight; Myosin Subfragments; Myosins; Peptide Fragments; Potassium Chloride; Protein Binding; Trypsin; Urea | 1985 |
[Clinical study of the new Dontisolon M].
Topics: Anti-Infective Agents; Chymotrypsin; Drug Combinations; Endopeptidases; Mouth Diseases; Neomycin; Prednisolone; Trypsin; Urea | 1988 |
Unfolding free energy changes determined by the linear extrapolation method. 1. Unfolding of phenylmethanesulfonyl alpha-chymotrypsin using different denaturants.
Topics: Binding Sites; Chymotrypsin; Guanidine; Guanidines; Kinetics; Mathematics; Methylurea Compounds; Phenylmethylsulfonyl Fluoride; Protein Conformation; Protein Denaturation; Solvents; Thermodynamics; Urea | 1988 |
Affinity chromatography on immobilized anhydrotrypsin: general utility for selective isolation of C-terminal peptides from protease digests of proteins.
Topics: Adsorption; alpha 1-Antitrypsin; Amino Acids; Bacterial Proteins; Chemical Phenomena; Chemistry; Chromatography, Affinity; Chromatography, High Pressure Liquid; Chymotrypsin; Enzymes, Immobilized; Peptide Fragments; Peptide Hydrolases; Trypsin; Urea | 1987 |
Computer-aided design of artificial enzymes: cyclic urea mimetics of alpha-chymotrypsin.
Topics: Chymotrypsin; Computer Graphics; Computer Simulation; Enzymes; Models, Molecular; Molecular Conformation; Protein Conformation; Urea | 1986 |
Developmental study of human fetal placental fibronectin: alterations in carbohydrates of tissue fibronectin during gestation.
Topics: Amniotic Fluid; Carbohydrate Metabolism; Chromatography; Chymotrypsin; Electrophoresis, Polyacrylamide Gel; Female; Fibronectins; Gelatin; Gestational Age; Humans; Molecular Weight; Peptide Fragments; Placenta; Pregnancy; Urea | 1987 |
Antigenic determinants in bovine alpha-chymotrypsin.
Topics: Amino Acids; Animals; Antigens; Cattle; Chymotrypsin; Cross Reactions; Epitopes; Histidine; Immune Sera; Ketones; Methionine; Precipitin Tests; Serine; Sulfonamides; Toluene; Urea | 1970 |
Effect of various proteolytic enzymes on fibrinogen.
Topics: Acrylates; Animals; Blood Coagulation Tests; Caseins; Cattle; Chymotrypsin; Electrophoresis, Disc; Fibrinogen; Fibrinolysin; Pancreatic Elastase; Peptide Hydrolases; Polymers; Pronase; Thrombin; Trypsin; Urea | 1971 |
Carboxyterminal antigenic determinants of collagen from calf skin. Localization within discrete regions of the nonhelical sequence.
Topics: Amino Acids; Animals; Antibody Specificity; Carboxypeptidases; Cattle; Chromatography, Gel; Chymotrypsin; Collagen; Cyanogen Bromide; Epitopes; Hemagglutination Inhibition Tests; Peptides; Rabbits; Skin; Trypsin; Tyrosine; Urea | 1972 |
A prestaining method for the quantitative assay of proteins on polyacrylamide gels.
Topics: Amino Acids; Animals; Cattle; Chromatography, Thin Layer; Chymotrypsin; Cyanogen Bromide; Electrophoresis, Disc; Evaluation Studies as Topic; Humans; Insulin; Methods; Muramidase; Proteins; Serum Albumin, Bovine; Spectrophotometry; Staining and Labeling; Time Factors; Transferrin; Urea | 1973 |
A high resolution PAS stain for polyacrylamide gel electrophoresis.
Topics: Acetates; Amylases; Animals; Chymotrypsin; Densitometry; Electrophoresis, Polyacrylamide Gel; Gels; In Vitro Techniques; Methods; Oxidation-Reduction; Periodic Acid; Proteins; Rats; Staining and Labeling; Submandibular Gland; Sulfhydryl Compounds; Trypsin; Urea | 1973 |
Electron microscopic studies of Escherichia coli ribosomal subunits.
Topics: Bacterial Proteins; Cell Fractionation; Centrifugation, Density Gradient; Chymotrypsin; Escherichia coli; Evaluation Studies as Topic; Magnesium; Methods; Microscopy, Electron; Nucleic Acid Conformation; Organoids; Ribosomes; RNA, Bacterial; RNA, Ribosomal; Spectrophotometry, Ultraviolet; Staining and Labeling; Urea | 1973 |
[Studies on the question of the immune globulin synthesis by human lymphocytes stimulated in vitro by phytohemagglutinin. II. Radiochemical studies].
Topics: Aspergillus; Blood Proteins; Carbon Isotopes; Carboxypeptidases; Chromatography, Thin Layer; Chymotrypsin; Culture Techniques; Freezing; gamma-Globulins; Humans; Hydrolases; Lectins; Leucine; Lymphocytes; Phenylalanine; Radiochemistry; Tissue Extracts; Trichloroacetic Acid; Tritium; Tyrosine; Urea | 1968 |
Detection of multiple forms of proteolytic enzymes by starch gel electrophoresis.
Topics: Bromelains; Buffers; Chymotrypsin; Electrophoresis; Endopeptidases; Gels; Hydrogen-Ion Concentration; Lactates; Methods; Papain; Pepsin A; Peptide Hydrolases; Protein Hydrolysates; Staining and Labeling; Starch; Temperature; Trypsin; Urea | 1968 |
Studies of the heterogeneity of Streptomyces griseus protease. Isolation and characterization of an alkaline serine protease from commercial pronase-P derived from Streptomyces griseus K1.
Topics: Amino Acids; Calcium; Chromatography, Gel; Chromatography, Ion Exchange; Chymotrypsin; Electrophoresis, Polyacrylamide Gel; Freeze Drying; Hydrogen-Ion Concentration; Isoflurophate; Molecular Weight; Pancreatic Elastase; Peptide Hydrolases; Phosphorus Radioisotopes; Pronase; Protease Inhibitors; Serine; Streptomyces griseus; Temperature; Time Factors; Urea | 1973 |
[Effect of denaturation of human gastric juice on the levels of vitamin B12 acceptors].
Topics: Chemical Phenomena; Chemistry; Chymotrypsin; Gastric Juice; Humans; Protein Denaturation; Proteins; Salicylates; Toluene; Trypsin; Urea; Vitamin B 12 | 1968 |
The effect of protein precipitants on alpha-chymotrypsin and other enzymes.
Topics: Animals; Binding Sites; Catalase; Cattle; Chemical Precipitation; Chromatography, Gel; Chromatography, Ion Exchange; Chymotrypsin; Dialysis; Esterases; Ethanol; Fructose-Bisphosphate Aldolase; Humans; Hydrochloric Acid; Kinetics; L-Lactate Dehydrogenase; Nephrosis; Pepsin A; Picrates; Rabbits; Ribonucleases; Salicylates; Solubility; Trichloroacetic Acid; Trypsin; Urea | 1970 |
Differential inhibition of attachment and eclipse activities of HeLa cells for enteroviruses.
Topics: Binding Sites; Chymotrypsin; Detergents; Enterovirus; Enterovirus B, Human; Freezing; Glycine; HeLa Cells; Hot Temperature; Hydrogen-Ion Concentration; Lithium; Poliovirus; Urea; Virus Replication | 1969 |
Urea denaturation of active-site spin-labeled -chymotrypsin.
Topics: Binding Sites; Chymotrypsin; Cyclic N-Oxides; Electron Spin Resonance Spectroscopy; Fluorine; Hydrogen-Ion Concentration; Organophosphorus Compounds; Piperidines; Protein Denaturation; Pyrroles; Urea | 1972 |
Ultrastructural characterization of genome of epizootic hemorrhagic disease virus.
Topics: Animals; Arboviruses; Centrifugation, Density Gradient; Chymotrypsin; Cricetinae; Deoxyuridine; DNA, Viral; Hemorrhagic Fevers, Viral; Hot Temperature; Kidney; Microscopy, Electron, Scanning; Perchlorates; Ribonucleases; RNA, Viral; Spectrophotometry; Urea; Virus Cultivation | 1973 |
Enzymatic digestion of the first component of human complement (C1q).
Topics: Antigen-Antibody Complex; Autoradiography; Chymotrypsin; Complement System Proteins; Electrophoresis, Disc; Hemolysis; Humans; Immunodiffusion; Immunoelectrophoresis; Iodine Radioisotopes; Isotope Labeling; Microbial Collagenase; Ovalbumin; Pancreatic Elastase; Sodium Dodecyl Sulfate; Trypsin; Urea | 1974 |
The heme environment in ferric and ferrous cytochrome c oxidase.
Topics: Animals; Chemical Phenomena; Chemistry; Chymotrypsin; Circular Dichroism; Cytochrome c Group; Electron Transport Complex IV; Ethylenes; Glycerol; Glycols; Heme; Horses; Iron; Myocardium; Oxidation-Reduction; Peptide Fragments; Protein Conformation; Spectrophotometry; Sucrose; Surface-Active Agents; Ultracentrifugation; Urea | 1974 |
Primary structure of the Mcg lambda chain.
Topics: Amino Acid Sequence; Amino Acids; Bence Jones Protein; Binding Sites; Chromatography, Gel; Chromatography, Ion Exchange; Chymotrypsin; Crystallization; Glycine; Humans; Hydrogen-Ion Concentration; Iodoacetates; Macromolecular Substances; Mercaptoethanol; Peptide Fragments; Peptides; Protein Binding; Spectrophotometry; Spectrophotometry, Ultraviolet; Thermolysin; Threonine; Trypsin; Urea | 1974 |
Urea and guanidine hydrochloride denaturation of ribonuclease, lysozyme, alpha-chymotrypsin, and beta-lactoglobulin.
Topics: Animals; Binding Sites; Calorimetry; Chemical Phenomena; Chemistry, Physical; Chymotrypsin; Disulfides; Female; Goats; Guanidines; Hydrogen-Ion Concentration; Kinetics; Lactoglobulins; Mathematics; Muramidase; Optical Rotation; Pregnancy; Protein Binding; Protein Conformation; Protein Denaturation; Ribonucleases; Spectrophotometry, Ultraviolet; Thermodynamics; Urea | 1974 |
Some observations on liver cell proliferation in the isolated perfused rat liver.
Topics: Animals; Chymotrypsin; DNA; DNA Replication; In Vitro Techniques; Liver; Liver Regeneration; Male; Perfusion; Phenols; Rats; Thymidine; Time Factors; Tritium; Urea | 1974 |
Fluorescent labeling of proteins and a plasma membrane using cycloheptaamylose-dansyl chloride complex.
Topics: Amylose; Animals; Binding Sites; Cattle; Cell Membrane; Chymotrypsin; Dansyl Compounds; Evaluation Studies as Topic; Liver; Methods; Ovalbumin; Protein Binding; Proteins; Rats; Serum Albumin, Bovine; Solubility; Spectrometry, Fluorescence; Spectrophotometry, Infrared; Temperature; Time Factors; Urea | 1974 |
The trypsin and chymotrypsin inhibitory capacity of human and bovine alpha-crystallin.
Topics: Animals; Binding Sites; Cataract; Cattle; Chromatography, DEAE-Cellulose; Chromatography, Gel; Chromatography, Ion Exchange; Chymotrypsin; Crystallins; Humans; Hydrogen-Ion Concentration; Kinetics; Lens, Crystalline; Macromolecular Substances; Molecular Weight; Protein Binding; Species Specificity; Tissue Extracts; Trypsin Inhibitors; Urea | 1974 |
Functional significance and location of cysteine residues in the molecule of aspartate aminotransferase.
Topics: Amino Acid Sequence; Amino Acids; Aspartate Aminotransferases; Benzoates; Binding Sites; Carbon Radioisotopes; Chloromercuribenzoates; Chromatography, Gel; Chromatography, Paper; Chymotrypsin; Cyanogen Bromide; Cysteine; Cytoplasm; Disulfides; Electrophoresis, Paper; Free Radicals; Iodoacetates; Nitro Compounds; Peptide Fragments; Protein Binding; Protein Conformation; Spectrophotometry, Ultraviolet; Trypsin; Urea | 1974 |
Microanalysis of proteins and peptides. I. Enhancement of the fluorescence intensity of dansyl amino acids and dansyl proteins in aqueous media and its application to assay of amino acids and proteins.
Topics: Amino Acids; Amylose; Chymotrypsin; Dansyl Compounds; Muramidase; Protamines; Proteins; Serum Albumin, Bovine; Spectrometry, Fluorescence; Thermodynamics; Urea | 1974 |
Microanalysis of proteins and peptides. II. Fluorescent labeling of proteins and a plasma membrane using cyclohepta-amylose-dansyl chloride complex.
Topics: Amylose; Animals; Cell Membrane; Chymotrypsin; Dansyl Compounds; Liver; Muramidase; Ovalbumin; Proteins; Rats; Serum Albumin, Bovine; Urea | 1974 |
The reactivity of the thiol groups of calf thymus deoxyribonucleohistone.
Topics: Animals; Benzoates; Binding Sites; Cattle; Chymotrypsin; Cysteine; Densitometry; Disulfides; DNA; Electrophoresis, Polyacrylamide Gel; Histones; Nitro Compounds; Nucleic Acid Conformation; Osmolar Concentration; Phosphates; Protein Binding; Protein Conformation; Sodium Chloride; Sodium Hydroxide; Solubility; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds; Thymus Gland; Time Factors; Urea | 1974 |
Kinetics of the proteolysis of aspartate aminotransferase in various states of the active site.
Topics: Animals; Aspartate Aminotransferases; Aspartic Acid; Binding Sites; Chymotrypsin; Circular Dichroism; Drug Stability; Isomerism; Kinetics; Myocardium; Protein Binding; Protein Conformation; Spectrophotometry; Spectrophotometry, Ultraviolet; Swine; Urea | 1974 |
Conformational studies on proteins by isoelectric focusing.
Topics: Animals; Cattle; Chymotrypsin; Concanavalin A; Hemoglobins; Humans; Insulin; Isoelectric Focusing; Mathematics; Pancreas; Plant Lectins; Plants; Protein Binding; Protein Conformation; Proteins; Ribonucleases; Serum Albumin; Spectrophotometry, Ultraviolet; Tosyl Compounds; Urea | 1973 |
Proton Fourier transform NMR studies of the unfolding of ribonuclease.
Topics: Animals; Binding Sites; Cattle; Chymotrypsin; Deuterium; Dioxins; Fourier Analysis; Guanidines; Hot Temperature; Hydrogen-Ion Concentration; Magnetic Resonance Spectroscopy; Models, Structural; Pancreas; Protein Binding; Protein Conformation; Protein Denaturation; Ribonucleases; Temperature; Trypsin; Urea | 1973 |
Evidence for nucleation in the folding of reduced hen egg lysozyme.
Topics: Animals; Autolysis; Chickens; Chromatography, Paper; Chymotrypsin; Copper; Dithiothreitol; Female; Hydrolysis; Muramidase; Ovum; Oxidation-Reduction; Pepsin A; Protein Conformation; Sulfhydryl Compounds; Urea | 1973 |
The estimation of polypeptide chain molecular weight by thin-layer gel filtration in urea and sodium dodecyl sulphate solution.
Topics: Alkylation; Animals; Cattle; Chromatography, Gel; Chymotrypsin; Densitometry; Evaluation Studies as Topic; Hemoglobins; Immunoglobulin Fragments; Macromolecular Substances; Methods; Molecular Conformation; Molecular Weight; Muramidase; Ovalbumin; Oxidation-Reduction; Pepsin A; Peptides; Protein Conformation; Proteins; Regression Analysis; Serum Albumin, Bovine; Sodium Dodecyl Sulfate; Trypsin; Urea | 1972 |
Enzymatic properties of microcapsules containing asparaginase.
Topics: Animals; Asparaginase; Capsules; Cattle; Chymotrypsin; Drug Stability; Hydrogen-Ion Concentration; Kinetics; Nylons; Pancreas; Polymers; Pronase; Proteus vulgaris; Temperature; Time Factors; Trypsin; Urea | 1973 |
The amino acid sequence of human plasma prealbumin.
Topics: Amino Acid Sequence; Amino Acids; Binding Sites; Blood Protein Electrophoresis; Carboxypeptidases; Chromatography, Gel; Chromatography, Ion Exchange; Chymotrypsin; Electrophoresis, Paper; Electrophoresis, Polyacrylamide Gel; Guanidines; Humans; Hydrazines; Molecular Weight; Pepsin A; Protein Binding; Protein Precursors; Serum Albumin; Spectrophotometry, Ultraviolet; Trypsin; Urea | 1974 |
Chymotrypsin inhibitor I from potatoes. Large scale preparation and characterization of its subunit components.
Topics: Amino Acids; Ammonium Sulfate; Animals; Chromatography, Gel; Chromatography, Ion Exchange; Chymotrypsin; Cross Reactions; Crystallization; Drug Stability; Electrophoresis; Formates; Guanidines; Hot Temperature; Hydrogen-Ion Concentration; Immunodiffusion; Immunoelectrophoresis; Macromolecular Substances; Molecular Weight; Plant Proteins; Plants; Rabbits; Trypsin Inhibitors; Urea | 1972 |
Further studies on mitogenic components of Phaseolus vulgaris phytohemagglutinin: subunit structure.
Topics: Amino Acids; Carboxypeptidases; Chromatography, Paper; Chymotrypsin; Dansyl Compounds; Electrophoresis, Disc; Electrophoresis, Paper; Hexosamines; Hexoses; Humans; Lectins; Lymphocyte Activation; Macromolecular Substances; Molecular Weight; Sodium Dodecyl Sulfate; Trypsin; Urea | 1972 |
Non-helical regions in rat collagen 1-chain.
Topics: Amino Acids; Animals; Chromatography, Gel; Chromatography, Ion Exchange; Chymotrypsin; Collagen; Cyanogen Bromide; Electrophoresis, Polyacrylamide Gel; Guanidines; Male; Peptides; Protein Conformation; Protein Denaturation; Rats; Skin; Sodium Dodecyl Sulfate; Tail; Tendons; Trypsin; Urea | 1972 |
Isolation and structural studies on synaptic complexes from rat brain.
Topics: Acetates; Animals; Brain; Brain Chemistry; Cell Fractionation; Centrifugation, Density Gradient; Chymotrypsin; Glycols; Male; Microscopy, Electron; Nerve Tissue Proteins; Rats; Sodium Chloride; Surface-Active Agents; Synaptic Membranes; Trypsin; Urea | 1972 |
Inactivation of the first component of human complement by m-(o-(2-chloro-5-fluorosulfonylphenylureido)-phenoxybutoxy)benzamidine.
Topics: Amidines; Animals; Binding Sites, Antibody; Blood Proteins; Calcium; Chromatography, Affinity; Chymotrypsin; Complement Fixation Tests; Complement Inactivator Proteins; Ethers; Guinea Pigs; Humans; Isoflurophate; Kinetics; Magnesium; Mathematics; Protein Binding; Rabbits; Sheep; Sulfones; Time Factors; Trypsin Inhibitors; Urea | 1972 |
On the stability of bovine superoxide dismutase. The effects of metals.
Topics: Animals; Apoproteins; Cations, Divalent; Cattle; Chymotrypsin; Drug Stability; Erythrocytes; Guanidines; Hydrogen-Ion Concentration; Metals; Oxidoreductases; Protein Denaturation; Sodium Dodecyl Sulfate; Solvents; Superoxide Dismutase; Temperature; Time Factors; Trypsin; Urea | 1973 |
Increased lysine hydroxylation in rat bone and tendon collagen and localization of the additional residues.
Topics: Amino Acids; Animals; Bone and Bones; Chromatography, Gel; Chromatography, Ion Exchange; Chymotrypsin; Collagen; Cyanogen Bromide; Guanidines; Hydroxylysine; Macromolecular Substances; Male; Peptides; Protein Conformation; Rats; Skin; Tendons; Urea | 1973 |
Conformation measurements on bovine trypsin and alpha-chymotrypsin during photodynamic inactivation.
Topics: Animals; Cattle; Chromatography, Ion Exchange; Chymotrypsin; Coloring Agents; Fluorescence; Hot Temperature; Oxidation-Reduction; Photochemistry; Protein Conformation; Trypsin; Tryptophan; Urea | 1973 |
Is -chymotrypsin an autolytic product in the activation scheme of chymotrypsinogen A?
Topics: Animals; Cattle; Chymotrypsin; Chymotrypsinogen; Enzyme Activation; Enzyme Precursors; Kinetics; Pancreas; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Time Factors; Urea | 1973 |
Selective cleavage of one disulfide bond in insulin: preparation and properties of insulin A7-B7-di-S-sulfonate.
Topics: Amino Acids; Animals; Binding Sites; Biological Assay; Blood Glucose; Cattle; Chromatography, Gel; Chromatography, Ion Exchange; Chymotrypsin; Disulfides; Electrophoresis, Paper; Fasting; Guinea Pigs; Hydrogen-Ion Concentration; Insulin; Mice; Passive Cutaneous Anaphylaxis; Peptide Fragments; Protein Binding; Protein Conformation; Rats; Sulfonic Acids; Urea | 1973 |
Chromophore labels in proteins. II. pH-dependence of circular dichroic spectra and differential perturbation spectra of alpha-chymotrypsin.
Topics: Chromatography, Gel; Chromatography, Ion Exchange; Chymotrypsin; Circular Dichroism; Ethylenes; Glycols; Hydrogen-Ion Concentration; Protein Conformation; Spectrophotometry, Ultraviolet; Urea | 1974 |
Unusual difference spectra of proteins containing tryptophan. II. Studies with proteins.
Topics: Chemical Phenomena; Chemistry; Chymotrypsin; Enzyme Precursors; Lactoglobulins; Muramidase; Pepsin A; Protein Denaturation; Proteins; Serum Albumin, Bovine; Spectrophotometry; Tryptophan; Ultraviolet Rays; Urea | 1969 |
A factor promoting dissociation of Escherichia coli ribosomes.
Topics: Ammonium Chloride; Chromatography, DEAE-Cellulose; Chymotrypsin; Cytochromes; Electrophoresis, Disc; Enzyme Precursors; Escherichia coli; Mercaptoethanol; Molecular Weight; Myoglobin; Ovalbumin; Ribosomes; Serum Albumin, Bovine; Urea | 1970 |
Oral effects of hydrolytic enzymes on taste acuity in man.
Topics: Adolescent; Adult; Amylases; Bacteria; Carboxypeptidases; Chymotrypsin; Egg White; Humans; Hyaluronoglucosaminidase; Intestines; Lipase; Male; Mouth; Muramidase; Pancreas; Papain; Pepsin A; Peptide Hydrolases; Ribonucleases; Taste; Testis; Triticum; Trypsin; Urea | 1971 |
Disulfide bonds of pepsinogen and pepsin: identification of the disulfide bonds which can be reversibly reduced and reoxidized.
Topics: Acetamides; Alkylation; Amino Acid Sequence; Amino Acids; Animals; Carbon Isotopes; Catalysis; Chemical Phenomena; Chemistry; Chromatography, Ion Exchange; Chromatography, Paper; Chymotrypsin; Cysteine; Disulfides; Electrophoresis, Paper; Enzyme Activation; Iodoacetates; Mercaptoethanol; Oxidation-Reduction; Pepsin A; Pepsinogens; Peptides; Protein Denaturation; Sulfides; Swine; Trypsin; Urea | 1971 |
The primary structure of the polypeptide chain of A II component of adult chicken hemoglobin.
Topics: Amino Acid Sequence; Amino Acids; Animals; Carboxypeptidases; Chickens; Chromatography, Ion Exchange; Chymotrypsin; Formates; Globins; Hemoglobins; Macromolecular Substances; Nitro Compounds; Oxidation-Reduction; Pepsin A; Peptides; Species Specificity; Trypsin; Urea | 1971 |
Immunochemical studies on alpha-amylase. I. Effects of denaturing agents and proteolytic enzymes on the immunochemical reactivity of alpha-amylase from Aspergillus oryzae.
Topics: Amylases; Aspergillus; Carboxypeptidases; Chromatography, Gel; Chymotrypsin; Edetic Acid; Immunodiffusion; Immunoelectrophoresis; In Vitro Techniques; Leucyl Aminopeptidase; Mercaptoethanol; Papain; Pepsin A; Peptide Hydrolases; Trypsin; Urea | 1965 |
Effect of calcium ion on S-100, a protein of the nervous system.
Topics: Acrylates; Animals; Antigens; Brain Chemistry; Calcium; Cattle; Chemical Phenomena; Chemistry; Chymotrypsin; Cysteine; Electrophoresis; Fluorometry; Immune Sera; Immunodiffusion; Magnesium; Methods; Nerve Tissue Proteins; Optical Rotatory Dispersion; Phenylalanine; Potassium; Protein Binding; Protein Denaturation; Quaternary Ammonium Compounds; Rabbits; Sodium; Solubility; Spectrophotometry; Strontium; Sulfhydryl Compounds; Time Factors; Tryptophan; Tyrosine; Urea; Viscosity | 1969 |
Studies on hog intrinsic factor. II. Immunoradioautographic studies of the properties of hog intrinsic factor and evidences suggesting the possible existence in hog tissues of hog intrinsic factor.
Topics: Acetone; Animals; Antigens; Autoradiography; Bile; Chymotrypsin; Endopeptidases; Ethanol; Hot Temperature; Immune Sera; Immunodiffusion; Intestines; Intrinsic Factor; Kidney; Liver; Spleen; Stomach; Swine; Thyroid Gland; Trypsin; Urea | 1970 |
Studies on the conidia of Aspergillus oryzae. XI. Latent ribonuclease in the conidia of Aspergillus oryzae.
Topics: Acetates; Aspergillus; Chloromercuribenzoates; Chromatography, DEAE-Cellulose; Chromatography, Gel; Chymotrypsin; Culture Media; Edetic Acid; Enzyme Activation; Freezing; Hot Temperature; Hydrogen-Ion Concentration; Hydrolysis; Kinetics; Lead; Molecular Weight; Peptide Hydrolases; Pronase; Ribonucleases; Ribosomes; RNA; Sodium Chloride; Subcellular Fractions; Trypsin; Urea; Yeasts | 1971 |
Glucagon structure and function. I. Purification and properties of bovine glucagon and monodesmidoglucagon.
Topics: Amino Acids; Animals; Asparagine; Biological Assay; Blood Glucose; Carboxypeptidases; Cattle; Chromatography, DEAE-Cellulose; Chromatography, Ion Exchange; Chymotrypsin; Circular Dichroism; Electrophoresis, Disc; Glucagon; Glutamine; Peptides; Protein Conformation; Rabbits; Radioimmunoassay; Swine; Ultraviolet Rays; Urea | 1972 |
Influence of anionic surfactants and ionic strength on -chymotrypsin-catalyzed reactions.
Topics: Acetates; Acrylates; Acylation; Benzene Derivatives; Chemical Phenomena; Chemistry; Chymotrypsin; Enzyme Activation; Fatty Alcohols; Indoles; Kinetics; Magnesium; Osmolar Concentration; Protein Binding; Sodium Chloride; Sodium Dodecyl Sulfate; Spectrophotometry; Sulfonic Acids; Surface-Active Agents; Time Factors; Tyrosine; Ultraviolet Rays; Urea | 1972 |
Air-reoxidation of reduced, denatured chymotrypsinogen A covalently attached to a solid matrix.
Topics: Air; Amino Acids; Animals; Benzoates; Catalysis; Cattle; Chromatography, Affinity; Chymotrypsin; Chymotrypsinogen; Enzyme Activation; Esters; Glass; Kinetics; Oxidation-Reduction; Protein Binding; Protein Denaturation; Succinates; Trypsin; Tyrosine; Urea | 1972 |
Studies on the nature of the "microfibrillar" component of elastic fibers.
Topics: Age Factors; Amino Acids; Animals; Carbohydrates; Cattle; Chemical Phenomena; Chemistry; Chymotrypsin; Cross Reactions; Elastin; Glycopeptides; Hexoses; Iodine Isotopes; Ligaments; Mercaptoethanol; Microscopy, Electron; Urea | 1971 |
The selective S-methylation of sulfhydryl groups in proteins and peptides with methyl-p-nitrobenzenesulfonate.
Topics: Amino Acids; Ammonia; Animals; Binding Sites; Carbon Isotopes; Cattle; Chemical Phenomena; Chemistry; Chromatography, Gel; Chymotrypsin; Cysteine; Esters; Glutathione; Hydrogen-Ion Concentration; Insulin; Kinetics; Mercaptoethanol; Methods; Methylation; Muramidase; Nitrobenzenes; Oxidation-Reduction; Papain; Peptides; Protein Denaturation; Proteins; Ribonucleases; Sodium; Sulfhydryl Compounds; Sulfonic Acids; Temperature; Transferases; Urea | 1971 |
Hemoglobin Atago (alpha2-85Tyr beta-2) a new abnormal human hemoglobin found in Nagasaki. Biochemical studies on hemoglobins and myoglobins. VI.
Topics: Amino Acids; Animals; Aspartic Acid; Chromatography, Ion Exchange; Chromatography, Paper; Chymotrypsin; Dogs; Electrophoresis; Electrophoresis, Paper; Female; Globins; Hemoglobins; Hemoglobins, Abnormal; Humans; Hybridization, Genetic; Infant; Japan; Macromolecular Substances; Peptides; Trypsin; Tyrosine; Urea | 1971 |
Studies on the conformational isomers of deoxycytidylate aminohydrolase.
Topics: Allosteric Regulation; Aminohydrolases; Bromine; Carboxypeptidases; Chemical Phenomena; Chemistry; Chlorides; Chloromercuribenzoates; Chymotrypsin; Cytosine Nucleotides; Deoxyribonucleotides; Fluorometry; Kinetics; Magnesium; Protein Binding; Protein Conformation; Spectrophotometry; Succinimides; Sulfhydryl Compounds; Thymine Nucleotides; Trypsin; Ultraviolet Rays; Urea | 1971 |
Reduction of chymotrypsin A by dithiothreitol.
Topics: Acrylates; Chemical Phenomena; Chemistry; Chloromercuribenzoates; Chymotrypsin; Cinnamates; Dithiothreitol; Hydrogen-Ion Concentration; Indoles; Oxidation-Reduction; Urea | 1971 |
Amino acid compositions of the soluble tryptic peptides and chymotryptic peptides from the polypeptide chain of all component of chicken hemoglobin. Biochemical studies on hemoglobins and myoglobins. VII.
Topics: Amino Acids; Animals; Chickens; Chromatography, Ion Exchange; Chromatography, Paper; Chymotrypsin; Globins; Hemoglobins; Macromolecular Substances; Peptides; Trypsin; Urea | 1971 |
Changes in conformation of insolubilized trypsin and chymotrypsin, followed by fluorescence.
Topics: Alanine; Binding Sites; Carboxylic Acids; Chymotrypsin; Cyanogen Bromide; Hot Temperature; Hydrogen-Ion Concentration; Mathematics; Naphthalenes; Peptides; Polysaccharides; Protein Binding; Protein Conformation; Protein Denaturation; Solubility; Spectrometry, Fluorescence; Sulfonic Acids; Temperature; Toluidines; Trypsin; Trypsin Inhibitors; Tryptophan; Urea | 1971 |
[Characteristics of complexes of urea with proteins].
Topics: Amylases; Animals; Arginase; Catalase; Cattle; Chymotrypsin; Cytochromes; Enzymes; Hemoglobins; Hexokinase; Male; Muramidase; Ovalbumin; Peroxidases; Proteins; Ribonucleases; Serum Albumin; Serum Albumin, Bovine; Trypsin; Urea | 1971 |
Identification of the trypsin-reactive site of the Bowman-Birk soybean inhibitor.
Topics: Acetamides; Alkylation; Amino Acid Sequence; Amino Acids; Arginine; Binding Sites; Butanones; Chromatography, DEAE-Cellulose; Chromatography, Gel; Chymotrypsin; Cyclohexanes; Dansyl Compounds; Glycine max; Guanidines; Iodoacetates; Ketones; Lysine; Succinates; Trypsin; Trypsin Inhibitors; Urea | 1971 |
Reactions of human anti-gamma-globulin factors with digested and urea-reduced gamma-chains.
Topics: Antibodies, Anti-Idiotypic; Chemical Phenomena; Chemistry; Chymotrypsin; Hemagglutination Inhibition Tests; Humans; Immunoelectrophoresis; Peptide Hydrolases; Peptides; Trypsin; Ultracentrifugation; Urea | 1966 |
Phenacyl bromides as chromophoric reagents for alpha-chymotrypsin.
Topics: Acetophenones; Alkylating Agents; Alkylation; Bromine; Chemical Phenomena; Chemistry; Chymotrypsin; Cyanides; Hot Temperature; Hydrogen-Ion Concentration; Methionine; Methods; Protein Denaturation; Spectrophotometry; Spectrum Analysis; Sulfides; Ultraviolet Rays; Urea | 1969 |
The formation and activities of substituted phenacylchymotrypsins.
Topics: Acetophenones; Bromine; Chemical Phenomena; Chemistry; Chymotrypsin; Computers; Hydrogen-Ion Concentration; Ketones; Kinetics; Spectrophotometry; Sulfonic Acids; Time Factors; Urea | 1969 |
[Biological characteristics of urinary HCG, with special reference to the synthetic HCG. 3. Studies on inactivation of synthetic HCG].
Topics: Aminopeptidases; Animals; Biological Assay; Carboxypeptidases; Chorionic Gonadotropin; Chymotrypsin; Cyanates; Enzymes; Female; Hot Temperature; Potassium; Rats; Urea | 1969 |
Amino acid sequences of peptides from a chymotryptic digest of a urea-soluble protein fraction (U.S.3) from oxidized wool.
Topics: Amino Acid Sequence; Animals; Chromatography, Ion Exchange; Chromatography, Paper; Chymotrypsin; Electrophoresis; Oxidation-Reduction; Peptides; Solubility; Trypsin; Urea; Wool | 1969 |
A comparison of anisole and formanilide binding to chymotrypsin by potentiometric difference titrimetry.
Topics: Anilides; Benzene Derivatives; Binding Sites; Calcium Chloride; Chemical Phenomena; Chemistry; Chymotrypsin; Ethers; Fluorides; Formates; Hydrogen-Ion Concentration; Methods; Potassium Chloride; Potentiometry; Urea | 1970 |
Conformational changes in chymotrypsin detected with antibodies. II. Antibody-interactions with chemically modified and denatured chymotrypsins.
Topics: Adsorption; Alkylation; Antigen-Antibody Complex; Antigen-Antibody Reactions; Chymotrypsin; Enzyme Precursors; Immunochemistry; Isoflurophate; Protein Denaturation; Urea | 1970 |
The primary structure of a tetradecapeptide containing the cysteine residue linking gamma M-globulin subunits.
Topics: Alkylation; Amino Acid Sequence; Amino Acids; Blood Protein Electrophoresis; Carbon Isotopes; Chromatography, Gel; Chymotrypsin; Cysteine; Humans; Immunoglobulin M; Iodoacetates; Methods; Peptides; Trypsin; Ultracentrifugation; Urea | 1970 |
The partial specific volume of chymotrypsinogen A in aqueous urea solutions.
Topics: Chemical Phenomena; Chemistry; Chymotrypsin; Densitometry; Enzyme Precursors; Protein Binding; Protein Denaturation; Urea; Water | 1970 |
A convenient purification of porcine pancreatic lipase free of proteolytic activity.
Topics: Animals; Chromatography, Gel; Chymotrypsin; Electrophoresis; Fluorine; Lipase; Lipids; Membranes; Mercaptoethanol; Methods; Pancreas; Pancreatin; Peptide Hydrolases; Protein Binding; Solubility; Sulfonic Acids; Swine; Trypsin Inhibitors; Urea | 1970 |
On the structural stability and solvent denaturation of proteins. 3. Denaturation by the amides.
Topics: Alcohols; Amides; Animals; Cattle; Cetacea; Chymotrypsin; Cytochromes; Enzyme Precursors; Esters; Formamides; Glycols; Horses; Hydrogen-Ion Concentration; Indoles; Mathematics; Myocardium; Myoglobin; Optical Rotatory Dispersion; Protein Binding; Protein Denaturation; Solubility; Solvents; Spectrophotometry; Tryptophan; Urea | 1970 |
Solvent effects on reactions catalyzed by some proteolytic enzymes.
Topics: 1-Propanol; Acetone; Caseins; Chemical Phenomena; Chemistry, Physical; Chymotrypsin; Glycine; Hemoglobins; Hydrogen-Ion Concentration; Kinetics; Mathematics; Peptide Hydrolases; Protein Denaturation; Solvents; Trypsin; Urea | 1967 |
Structure and function of chloroplast proteins. II. Effect of p-chloromercuribenzoate treatment on the ribulose 1,5-diphosphate carboxylase activity of spinach leaf fraction I protein.
Topics: Carboxy-Lyases; Chloromercuribenzoates; Chromatography, Gel; Chromatography, Ion Exchange; Chymotrypsin; Cysteine; Detergents; Microscopy, Electron; Pentosephosphates; Peptide Hydrolases; Plant Proteins; Sulfhydryl Compounds; Urea | 1968 |
Acetoacetate decarboxylase. The molecular weight of the enzyme and subunits.
Topics: Acetoacetates; Birefringence; Carboxy-Lyases; Catalase; Chymotrypsin; Clostridium; Glycine; Guanidines; Hydrogen-Ion Concentration; Molecular Weight; Serum Albumin; Ultracentrifugation; Urea | 1968 |
Physico-chemical properties of bovine chymotrypsinogen B. A comparative study with trypsinogen and chymotrypsinogen A.
Topics: Animals; Binding Sites; Calcium; Cattle; Chymotrypsin; Enzyme Precursors; Flavins; Hydrogen-Ion Concentration; Indoles; Phenols; Protein Denaturation; Temperature; Thermodynamics; Trypsin; Urea | 1968 |
[Study on the biological characteristics of intra-trophoblastic gonadotropins].
Topics: Adult; Aminopeptidases; Animals; Carboxypeptidases; Chorionic Gonadotropin; Chymotrypsin; Electrophoresis; Female; Hemagglutination Inhibition Tests; Humans; Hydatidiform Mole; Hypophysectomy; Male; Organ Size; Ovary; Placenta; Pregnancy; Prostate; Proteins; Rats; Seminal Vesicles; Spectrum Analysis; Ultraviolet Rays; Urea | 1968 |
An analysis of perturbations in the ultraviolet absorption spectra of proteins and model compounds.
Topics: 1-Propanol; Chymotrypsin; Dioxins; Esters; Ethanol; Glycols; Muramidase; Protein Denaturation; Proteins; Serum Albumin; Serum Albumin, Bovine; Solvents; Spectrophotometry; Tryptophan; Tyrosine; Urea | 1968 |
Activation of chymotrypsinogen X and specificity of the enzyme.
Topics: Chromatography, Gel; Chymotrypsin; Enzyme Precursors; Esters; Fatty Acids; Hippurates; Kinetics; Protein Denaturation; Serum Albumin; Trypsin; Tyrosine; Ultracentrifugation; Urea | 1968 |
[Studies on trypsin and chmotrypsin chemically bound to carboxymethylcellulose].
Topics: Chemical Phenomena; Chemistry; Chymotrypsin; Hydrogen-Ion Concentration; Methylcellulose; Time Factors; Trypsin; Urea | 1968 |
The protection of alpha-chymotrypsin by hydrocinnamate from denaturation by ethanol and urea.
Topics: Chymotrypsin; Cinnamates; Ethanol; Methods; Urea | 1969 |
On the structural and functional role of carboxylates in chymotrypsinogen A: a comparison with chymotrypsin, trypsinogen and trypsin.
Topics: Binding Sites; Chemical Phenomena; Chemistry; Chymotrypsin; Drug Stability; Enzyme Precursors; Kinetics; Optical Rotatory Dispersion; Protein Denaturation; Trypsin; Trypsinogen; Urea | 1969 |
Coupling of p-nitrophenyl diazonium fluoroborate to bovine chymotrypsinogen A and alpha-chymotrypsin.
Topics: Animals; Boron Compounds; Caproates; Cattle; Chemical Phenomena; Chemistry; Chymotrypsin; Diazonium Compounds; Enzyme Precursors; Fluorine; Histidine; Kinetics; Lysine; Nitrophenols; Spectrum Analysis; Urea | 1969 |
The stability of chymotrypsin cross-linked with formaldehyde.
Topics: Catalysis; Chemical Phenomena; Chemistry; Chymotrypsin; Drug Stability; Formaldehyde; Hot Temperature; In Vitro Techniques; Propionates; Urea | 1964 |
The iodination of chymotrypsinogen.
Topics: Ammonia; Autoradiography; Chemical Phenomena; Chemistry; Chymotrypsin; Enzyme Precursors; Histidine; In Vitro Techniques; Iodine; Peptides; Tyrosine; Urea | 1964 |
Preparative zone electrophoresis of proteins on polyacrylamide gels in 8 M urea.
Topics: Acrylic Resins; Chymotrypsin; Cytochromes; Electrophoresis; Encephalitis Viruses; Hydrogen-Ion Concentration; Proteins; Tobacco Mosaic Virus; Trypsin; Urea; Viral Proteins | 1965 |
The activation of chymotrypsinogen. Optical rotation studies.
Topics: Chemical Phenomena; Chemistry, Physical; Chymotrypsin; Enzyme Precursors; Protein Denaturation; Urea | 1965 |
[Changes in the conformation of alpha-chymotrypsin and chymotrypsinogen A in the presence of caprinate].
Topics: Caproates; Chymotrypsin; Solutions; Trypsinogen; Urea | 1965 |
The effect of osmotic agents employed before cataract extraction. On the position of the vitreous following removal of the lens.
Topics: Acetazolamide; Cataract Extraction; Chymotrypsin; Glycerides; Humans; Hypertonic Solutions; Intraocular Pressure; Mannitol; Urea; Vitreous Body | 1966 |
Unfolding reactions of proteins. I. Kinetic and equilibrium measurements of diisopropylphosphorylchymotrypsin and chymotrypsinogen in urea.
Topics: Chemical Phenomena; Chemistry, Physical; Chymotrypsin; Enzyme Precursors; Kinetics; Protein Denaturation; Solutions; Spectrophotometry; Thermodynamics; Urea | 1966 |
Isolation of the B and C chains from S-sulfonated alpha-chymotrypsin.
Topics: Amino Acids; Autoanalysis; Chromatography; Chromatography, Gel; Chymotrypsin; Enzyme Precursors; Peptides; Phosphorus Isotopes; Serine; Solubility; Spectrophotometry; Sulfites; Trichloroacetic Acid; Urea | 1966 |
Location of the heme moiety of cytochrome c by solvent perturbation.
Topics: Chymotrypsin; Cytochromes; Glycols; Heme; Models, Theoretical; Solvents; Spectrophotometry; Urea; Viscosity | 1967 |
Unfolding reactions of proteins. II. Spectral and optical rotatory dispersion studies in urea, guanidinium chloride, and 2-chloroethanol.
Topics: Chemical Phenomena; Chemistry, Physical; Chlorides; Chlorine; Chymotrypsin; Guanidines; Ions; Kinetics; Muramidase; Protein Denaturation; Solvents; Spectrophotometry; Temperature; Thermodynamics; Tryptophan; Urea | 1967 |
Fractionation of the chymotryptic precipitate of Bombyx mori silk fibroin.
Topics: Amino Acids; Animals; Bombyx; Chromatography, Gel; Chromatography, Ion Exchange; Chymotrypsin; Dialysis; Dinitrophenols; Electrophoresis; Fibroins; Molecular Weight; Ultracentrifugation; Urea | 1967 |
Denaturation of proteins in 8 M urea as monitored by tryptophan fluorescence: chymotrypsin, chymotrypsinogen and some derivatives.
Topics: Chymotrypsin; Enzyme Precursors; Fluorometry; Hydrogen-Ion Concentration; Isoflurophate; Kinetics; Protein Denaturation; Urea | 1967 |
Kinetic study of protein unfolding and refolding using urea gradient electrophoresis.
Topics: Chymotrypsin; Chymotrypsinogen; Cytochrome c Group; Electrophoresis, Polyacrylamide Gel; Kinetics; Lactalbumin; Lactoglobulins; Micrococcal Nuclease; Muramidase; Protein Conformation; Ribonucleases; Urea | 1980 |
Uncoating-like modification of poliovirus capsid resulting from the cooperative effects of subfreezing temperature and submolar concentrations of urea.
Topics: Adsorption; Capsid; Chemical Phenomena; Chemistry; Chymotrypsin; Freezing; Genes, Viral; Isoelectric Point; Macromolecular Substances; Poliovirus; Urea; Viral Proteins; Water | 1982 |
Investigations about influence of the content of plant crude protein in the ration on the utilization of urea in dairy cattle. 6. Digestive enzymes and their interactions in contents of proximal small intestine.
Topics: alpha-Amylases; Animals; Carboxypeptidases; Cattle; Chymotrypsin; Dietary Proteins; Enzyme Activation; Female; Gastrointestinal Contents; Hydrogen-Ion Concentration; Intestinal Secretions; Intestine, Small; Pancreatic Elastase; Pepsin A; Plant Proteins; Trypsin; Urea | 1984 |
The primary structure of a human IgG2 heavy chain: genetic, evolutionary, and functional implications.
Topics: Amino Acid Sequence; Chymotrypsin; Cyanogen Bromide; Humans; Immunoglobulin G; Immunoglobulin Heavy Chains; Pepsin A; Peptides; Trypsin; Urea | 1980 |
Specificity and stability of the chymotrypsin inhibitor from winged bean seed (Psophocarpus tetragonolobus (L) Dc).
Topics: Animals; Cattle; Chromatography, Gel; Chymotrypsin; Electrophoresis, Polyacrylamide Gel; Fabaceae; Hydrogen-Ion Concentration; Mercaptoethanol; Molecular Weight; Pepsin A; Plants, Medicinal; Seeds; Substrate Specificity; Temperature; Urea | 1981 |
Discrimination of different domains in fibronectin on the basis of their stability against urea denaturation.
Topics: Chymotrypsin; Circular Dichroism; Cold Temperature; Fibronectins; Hot Temperature; Humans; Peptide Fragments; Protein Denaturation; Urea | 1983 |
Active site structural change of alpha-chymotrypsin due to 2-halogeno-ethanols; comparison with ethanol, I-propanol, and urea.
Topics: 1-Propanol; Binding Sites; Chymotrypsin; Ethanol; Ethylene Chlorohydrin; Kinetics; Structure-Activity Relationship; Trifluoroethanol; Urea | 1980 |
The chaperonin GroEL is destabilized by binding of ADP.
Topics: Adenosine Diphosphate; Chaperonin 60; Chymotrypsin; Light; Protein Binding; Protein Conformation; Scattering, Radiation; Spectrometry, Fluorescence; Tyrosine; Urea | 1995 |
Residual structure in urea-denatured chaperonin GroEL.
Topics: Anilino Naphthalenesulfonates; Chaperonin 60; Chymotrypsin; Circular Dichroism; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Fluorescence; Fluorescent Dyes; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Scattering, Radiation; Ultracentrifugation; Urea | 1995 |
Conformational stability of bovine holo and apo adrenodoxin--a scanning calorimetric study.
Topics: Adrenodoxin; Animals; Apoproteins; Ascorbic Acid; Calorimetry, Differential Scanning; Cattle; Chymotrypsin; Electrophoresis, Polyacrylamide Gel; Hydrogen-Ion Concentration; Mercaptoethanol; Protein Conformation; Protein Denaturation; Protein Folding; Spectrophotometry; Sulfides; Temperature; Thermodynamics; Urea | 1995 |
Hydrophobic surfaces that are hidden in chaperonin Cpn60 can be exposed by formation of assembly-competent monomers or by ionic perturbation of the oligomer.
Topics: Amino Acid Sequence; Anilino Naphthalenesulfonates; Chaperonin 60; Chymotrypsin; Escherichia coli; Fluorescent Dyes; Guanidine; Guanidines; Macromolecular Substances; Osmolar Concentration; Peptide Fragments; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Spectrometry, Fluorescence; Surface Properties; Ultracentrifugation; Urea | 1995 |
Local unfolding and the stepwise loss of the functional properties of tubulin.
Topics: Anilino Naphthalenesulfonates; Animals; Brain Chemistry; Chymotrypsin; Colchicine; Fluorescent Dyes; GTP Phosphohydrolases; Kinetics; Microtubules; Polymers; Protein Folding; Rats; Spectrometry, Fluorescence; Tropolone; Trypsin; Tryptophan; Tubulin; Urea | 1994 |
Potency and selectivity of inhibition of human immunodeficiency virus protease by a small nonpeptide cyclic urea, DMP 323.
Topics: Amino Acid Sequence; Aspartic Acid Endopeptidases; Azepines; Blood; Chymotrypsin; HIV Protease; HIV Protease Inhibitors; HIV-1; HIV-2; Humans; Kinetics; Molecular Sequence Data; Substrate Specificity; Urea | 1994 |
Erythrina caffra trypsin inhibitor retains its native structure and function after reducing its disulfide bonds.
Topics: Chymotrypsin; Disulfides; Drug Stability; Electrophoresis, Polyacrylamide Gel; Erythrina; Guanidine; Guanidines; Hydrogen-Ion Concentration; Kinetics; Molecular Weight; Oxidation-Reduction; Plant Proteins; Plants, Medicinal; Protein Denaturation; Protein Folding; Seeds; Spectrophotometry, Ultraviolet; Trypsin; Trypsin Inhibitors; Urea | 1994 |
Preparation and characterization of fragments from the N-terminal end of bovine serum albumin under native conditions.
Topics: Amino Acid Sequence; Chromatography, Gel; Chymotrypsin; Cross Reactions; Cyanogen Bromide; Electrophoresis, Polyacrylamide Gel; Glutathione; Glutathione Disulfide; Kinetics; Mercaptoethanol; Peptide Fragments; Protein Conformation; Serum Albumin, Bovine; Sulfhydryl Compounds; Trypsin; Urea; Viscosity | 1993 |
Correlation of high-temperature stability of alpha-chymotrypsin with 'salting-in' properties of solution.
Topics: Chymotrypsin; Enzyme Stability; Formamides; Guanidine; Guanidines; Hot Temperature; Kinetics; Models, Structural; Osmolar Concentration; Protein Conformation; Solutions; Thermodynamics; Thiocyanates; Urea | 1994 |
Structure of the alpha subunit of F1-ATPase probed by limited proteolysis.
Topics: Adenosine Triphosphate; Amino Acid Sequence; Bacillus; Chromatography, High Pressure Liquid; Chymotrypsin; Electrophoresis, Polyacrylamide Gel; Endopeptidases; Molecular Sequence Data; Peptide Fragments; Peptide Mapping; Proton-Translocating ATPases; Serine Endopeptidases; Thermolysin; Trypsin; Urea | 1993 |
Probes of energy transduction in enzyme catalysis.
Topics: Acylation; Catalysis; Chymotrypsin; Guanidine; Guanidines; Hydrogen-Ion Concentration; Kinetics; Models, Chemical; Protein Binding; Protein Folding; Spectrophotometry; Thermodynamics; Titrimetry; Urea | 1995 |
Substitution of serine for glycine-91 in the HXGH motif of CTP:phosphocholine cytidylyltransferase implicates this motif in CTP binding.
Topics: Animals; Base Sequence; Cell Line; Choline-Phosphate Cytidylyltransferase; Chymotrypsin; Glycine; Hydrolysis; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Nucleotidyltransferases; Protein Denaturation; Serine; Substrate Specificity; Urea | 1996 |
Probing protein structure using biochemical and biophysical methods. Proteolysis, matrix-assisted laser desorption/ionization mass spectrometry, high-performance liquid chromatography and size-exclusion chromatography of p21Waf1/Cip1/Sdi1.
Topics: Chromatography, Gel; Chromatography, High Pressure Liquid; Chymotrypsin; Cyclin-Dependent Kinase Inhibitor p21; Cyclins; Electrophoresis, Polyacrylamide Gel; Enzyme Inhibitors; Osmolar Concentration; Peptide Fragments; Peptide Hydrolases; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Subtilisins; Trypsin; Urea | 1997 |
Biochemical characterisation of an epitope on the surface membrane antigen (Cs-gp200) of the pathogenic piscine haemoflagellate Cryptobia salmositica Katz 1951.
Topics: Amidohydrolases; Animals; Antibodies, Monoclonal; Antigens, Protozoan; Antigens, Surface; Chymotrypsin; Endopeptidase K; Enzyme-Linked Immunosorbent Assay; Epitopes; Eukaryota; Glycoside Hydrolases; Hydrolysis; Oncorhynchus mykiss; Oxidation-Reduction; Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; Polysaccharides; Serine Endopeptidases; Trypsin; Type C Phospholipases; Urea | 1998 |
Retardation of thermal and urea induced inactivation of alpha-chymotrypsin by modification with carbohydrate polymers.
Topics: Carboxymethylcellulose Sodium; Chymotrypsin; Dextrans; Enzyme Activation; Enzyme Stability; Polymers; Protein Engineering; Serum Albumin, Bovine; Spectrometry, Fluorescence; Sucrose; Urea | 1998 |
Purification and characterization of a proteinase inhibitor from field bean, Dolichos lablab perpureus L.
Topics: Amino Acids; Benzoylarginine Nitroanilide; Chromatography, Gel; Chromatography, Ion Exchange; Chymotrypsin; Circular Dichroism; Fabaceae; Hydrogen-Ion Concentration; Isoelectric Focusing; Kinetics; Plant Proteins; Plants, Medicinal; Protease Inhibitors; Temperature; Trypsin; Urea | 1999 |
The tert-butyl hydroperoxide-induced oxidation of actin Cys-374 is coupled with structural changes in distant regions of the protein.
Topics: Actins; Animals; Chymotrypsin; Cross-Linking Reagents; Cyanogen Bromide; Cysteine; Hydrolysis; Kinetics; Lysine; Oxidants; Peptide Fragments; Polymers; Protein Conformation; Protein Denaturation; Rabbits; Spectrometry, Fluorescence; Subtilisins; Sulfhydryl Compounds; tert-Butylhydroperoxide; Tryptophan; Urea | 1999 |
Activation of enzymes for nonaqueous biocatalysis by denaturing concentrations of urea.
Topics: Acetone; Alcohol Dehydrogenase; Catalysis; Chymotrypsin; Dose-Response Relationship, Drug; Enzyme Activation; Freeze Drying; Furans; Hexanes; Horseradish Peroxidase; Protein Denaturation; Protein Folding; Solvents; Substrate Specificity; Subtilisins; Urea | 2001 |
Isolation of isoforms of mouse prion protein with PrP(SC)-like structural properties.
Topics: Animals; Chromatography, High Pressure Liquid; Chymotrypsin; Circular Dichroism; Endopeptidase K; Kinetics; Light; Mass Spectrometry; Mice; Molecular Weight; Oxidation-Reduction; Peptide Fragments; Plasmids; Protein Conformation; Protein Denaturation; Protein Folding; Protein Isoforms; Protein Structure, Secondary; PrPSc Proteins; Spectrophotometry; Urea | 2001 |
Effect of urea on trypsin and alpha-chymotrypsin.
Topics: Chymotrypsin; Endopeptidases; Hydrolases; Peptide Hydrolases; Trypsin; Urea | 1956 |
Structural changes in the activation of chymotrypsinogen and trypsinogen; effect of urea on chymotrypsinogen and delta-chymotrypsin.
Topics: Chymotrypsin; Chymotrypsinogen; Enzyme Precursors; Hydrolases; Peptide Hydrolases; Trypsin; Trypsinogen; Urea | 1956 |
The differentiation of two forms of chymotrypsin by their rates of urea denaturation.
Topics: Chymotrypsin; Urea | 1962 |
[NONSPECIFIC INFECTION OF THE URINARY TRACT. V. DEFENSE MECHANISMS OF THE HOST TO INFECTION].
Topics: Chymotrypsin; gamma-Globulins; Humans; Kanamycin; Lipoproteins; Methylprednisolone; Urea; Urinary Tract Infections | 1963 |
THE UREA DENATURATION OF ALPHA-CHYMOTRYPSIN. THE EFFECT OF PH ON REVERSIBLE AND IRREVERSIBLE INACTIVATION.
Topics: Chymotrypsin; Hydrogen-Ion Concentration; Research; Urea | 1963 |
THE UREA DENATURATION OF ALPHA-CHYMOTRYPSIN IN THE PRESENCE OF CALCIUM.
Topics: Calcium; Chemistry Techniques, Analytical; Chymotrypsin; Enzyme Inhibitors; Hydrogen-Ion Concentration; Research; Urea | 1963 |
[ON INFECTIOUS SUBSTRUCTURES FROM E. COLI BACTERIOPHAGES. 3. DEMONSTRATION AND PROPERTIES OF "HT2" PARTICLES].
Topics: Bacteriophages; Chymotrypsin; Coliphages; Deoxyribonucleases; DNA; Electrons; Escherichia coli; Immune Sera; Microscopy; Microscopy, Electron; Papain; Peptide Hydrolases; Rabbits; Research; Trypsin; Urea; Virus Cultivation | 1963 |
[THE GLYCOPROTEINS OF BRONCHIAL SECRETIONS].
Topics: Antibodies; Asthma; Beta-Globulins; Blood Group Antigens; Bronchitis; Chromatography; Chymotrypsin; Dialysis; Electrophoresis; Fucose; Glycoproteins; Hexosamines; Humans; Immunoelectrophoresis; Mucous Membrane; Mucus; Neuraminic Acids; Pentoses; Pepsin A; Renal Dialysis; Serum Globulins; Sputum; Transferrin; Trypsin; Urea | 1963 |
EFFECT OF X-RAYS, ULTRAVIOLET LIGHT, AND CHEMICALS ON THE KINETIC PARAMETERS OF CHYMOTRYPSIN.
Topics: Acetates; Acetylesterase; Chemical Phenomena; Chemistry; Chymotrypsin; Hot Temperature; Kinetics; Radiation Effects; Research; Ultraviolet Rays; Urea; X-Rays | 1964 |
PREFERENTIAL OXIDATION OF THE METHIONINE RESIDUE NEAR THE ACTIVE SITE OF CHYMOTRYPSIN.
Topics: Acetates; Autoradiography; Carbon Isotopes; Catalytic Domain; Chromatography; Chymotrypsin; Electrophoresis; Endopeptidases; Formates; Hydrogen Peroxide; Iodoacetates; Isoflurophate; Methionine; Peptides; Phosphorus Isotopes; Research; Urea | 1964 |
REVERSAL OF INSULIN RESISTANCE IN RED CELL SUGAR TRANSPORT.
Topics: Arabinose; Biological Transport; Carbohydrate Metabolism; Chymotrypsin; Erythrocytes; Fructose; Galactose; Glycerol; Insulin; Insulin Resistance; Lipase; Mannose; Permeability; Pharmacology; Phospholipases; Research; Ribose; Sorbose; Threonine; Urea; Valine; Xylose | 1964 |
INHIBITION OF TRYPSIN BY COPOLYMERS OF GLUTAMIC ACID AND OTHER AMINO ACIDS.
Topics: Amino Acids; Chemical Phenomena; Chemistry; Chymotrypsin; Colorimetry; Glutamates; Glutamic Acid; Leucine; Peptides; Phenylalanine; Research; Trypsin; Trypsin Inhibitors; Tyrosine; Urea | 1964 |
SULFONYL FLUORIDES AS INHIBITORS OF ESTERASES. II. FORMATION AND REACTIONS OF PHENYLMETHANESULFONYL ALPHA-CHYMOTRYPSIN.
Topics: Carbon Isotopes; Catalysis; Chymotrypsin; Dialysis; Dinitrophenols; Enzyme Inhibitors; Esterases; Fluorides; Hydrogen-Ion Concentration; Kinetics; Renal Dialysis; Research; Sulfinic Acids; Urea | 1964 |
ON THE CELLULAR FIXATION OF CYTOPHILIC ANTIBODY.
Topics: Anaphylaxis; Animals; Antibodies; Chymotrypsin; Lagomorpha; Lipase; Papain; Pharmacology; Phenols; Rabbits; Research; Solvents; Spleen; Surface-Active Agents; Trypsin; Urea | 1964 |
RECENT WORK ON THE CHEMISTRY AND BIOCHEMISTRY OF ANGIOTENSIN II.
Topics: Angiotensin II; Angiotensins; Animals; Chemical Phenomena; Chemistry; Chymotrypsin; Horses; Metabolism; Pepsin A; Physiology; Research; Swine; Trypsin; Urea | 1964 |
MECHANISM OF ENZYME CATALYSIS.
Topics: Adenosine Triphosphate; Catalysis; Chymotrypsin; Cinnamates; Cytosine Nucleotides; Enzymes; Metabolism; Muscle Proteins; Research; Ribonucleases; Urea; Urease | 1964 |
ALTERATIONS IN THE PHYSICAL AND ENZYMIC PROPERTIES OF ALPHA-CHYMOTRYPSIN IN UREA AND GUANIDINIUM CHLORIDE.
Topics: Calcium Chloride; Chemical Phenomena; Chemistry; Chymotrypsin; Guanidine; Guanidines; Hydrogen-Ion Concentration; Research; Spectrophotometry; Urea | 1964 |
STABILITY STUDIES ON LUTEINIZING HORMONE.
Topics: Amylases; Animals; Ascorbic Acid; Biological Assay; Carboxypeptidases; Cattle; Chemical Phenomena; Chemistry; Chymotrypsin; Clostridium; Cysteine; Dimercaprol; Female; Formates; Guanidines; Hot Temperature; Humans; Hydrogen-Ion Concentration; Luteinizing Hormone; Neuraminidase; Ovary; Peptide Hydrolases; Rats; Research; Sheep; Sheep, Domestic; Swine; Thiocyanates; Trypsin; Urea | 1964 |
The urea inactivation of alpha-chymotrypsin: alterations in enzymic and physical properties.
Topics: Chymotrypsin; Physical Phenomena; Urea | 1961 |
Enzyme-friendly, mass spectrometry-compatible surfactant for in-solution enzymatic digestion of proteins.
Topics: Alkanesulfonates; Angiotensin II; Arginine; Bacteriorhodopsins; Chromatography, High Pressure Liquid; Chymotrypsin; Dioxolanes; Endopeptidases; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; Membrane Proteins; Molecular Structure; Muramidase; Myoglobin; Neurotensin; Ovalbumin; Proteins; Sodium Dodecyl Sulfate; Solubility; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Surface-Active Agents; Trypsin; Ubiquitin; Urea | 2003 |
The peculiar nature of unfolding of the human prion protein.
Topics: Algorithms; Amyloid; Chromatography, Gel; Chymotrypsin; Circular Dichroism; Fluorescent Dyes; Hot Temperature; Humans; Peptide Fragments; Prions; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Spectrometry, Fluorescence; Thermodynamics; Time Factors; Urea | 2004 |
pH-responsive polymer-assisted refolding of urea- and organic solvent-denatured alpha-chymotrypsin.
Topics: Chymotrypsin; Dioxanes; Electrophoresis, Polyacrylamide Gel; Hydrogen-Ion Concentration; Polymethacrylic Acids; Protein Denaturation; Protein Renaturation; Urea | 2003 |
Osmolyte counteracts urea-induced denaturation of alpha-chymotrypsin.
Topics: Calorimetry, Differential Scanning; Chymotrypsin; Light; Methylamines; Protein Conformation; Protein Denaturation; Protein Structure, Tertiary; Scattering, Radiation; Temperature; Thermodynamics; Urea | 2009 |
Trehalose protects urea-induced unfolding of α-chymotrypsin.
Topics: Animals; Calorimetry, Differential Scanning; Cattle; Chymotrypsin; Models, Biological; Protein Unfolding; Transition Temperature; Trehalose; Urea | 2010 |
Concentration regimes and denaturation effects on the conformational changes of α-chymotrypsin by viscosity and dynamic light scattering measurements.
Topics: Acetonitriles; Animals; Cattle; Chemistry Techniques, Analytical; Chymotrypsin; Guanidine; Hydrodynamics; Light; Protein Conformation; Protein Denaturation; Scattering, Radiation; Solvents; Temperature; Urea; Viscosity | 2010 |
Resolving the native conformation of Escherichia coli OmpA.
Topics: Bacterial Outer Membrane Proteins; Blotting, Western; Chloroform; Chymotrypsin; Disulfides; Escherichia coli; Escherichia coli Proteins; Inclusion Bodies; Lipid Bilayers; Magnetic Resonance Spectroscopy; Models, Molecular; Periplasm; Protein Conformation; Protein Folding; Protein Structure, Tertiary; Temperature; Urea | 2010 |
A protic ionic liquid attenuates the deleterious actions of urea on α-chymotrypsin.
Topics: Chymotrypsin; Enzyme Stability; Ionic Liquids; Models, Molecular; Protein Denaturation; Urea | 2011 |
Refolding of urea-denatured α-chymotrypsin by protein-folding liquid chromatography.
Topics: Chromatography, Liquid; Chymotrypsin; Hydrophobic and Hydrophilic Interactions; Protein Denaturation; Protein Refolding; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Urea | 2013 |
Compensating effects of urea and trimethylamine-N-oxide on the heteroassociation of α-chymotrypsin and soybean trypsin inhibitor.
Topics: Chymotrypsin; Fluorescence; Glycine max; Hydrogen-Ion Concentration; Methylamines; Thermodynamics; Trypsin Inhibitor, Kunitz Soybean; Urea | 2013 |
New prenylated aeruginosin, microphycin, anabaenopeptin and micropeptin analogues from a Microcystis bloom material collected in Kibbutz Kfar Blum, Israel.
Topics: Aquaculture; Bacteriocins; Chromatography, High Pressure Liquid; Chymotrypsin; Depsipeptides; Dipeptides; Guanidines; Harmful Algal Bloom; Israel; Marine Toxins; Microcystis; Molecular Structure; Nuclear Magnetic Resonance, Biomolecular; Oligopeptides; Peptides, Cyclic; Ponds; Protein Prenylation; Serine Proteinase Inhibitors; Spectrometry, Mass, Electrospray Ionization; Stereoisomerism; Tandem Mass Spectrometry; Trypsin Inhibitors; Urea | 2015 |
Combined pressure and cosolvent effects on enzyme activity - a high-pressure stopped-flow kinetic study on α-chymotrypsin.
Topics: Biocatalysis; Chymotrypsin; Enzyme Activation; Hydrolysis; Kinetics; Methylamines; Peptides; Pressure; Solvents; Urea | 2015 |
Effect of Osmolytes and Guanidinium Chloride on the Enzymatic Properties of Bovine α-Chymotrypsin.
Topics: Animals; Betaine; Biocatalysis; Catalytic Domain; Cattle; Chymotrypsin; Guanidine; Kinetics; Methylamines; Peptides; Urea | 2016 |
Co-solvent effects on reaction rate and reaction equilibrium of an enzymatic peptide hydrolysis.
Topics: Animals; Calcium Chloride; Cattle; Chymotrypsin; Dimethyl Sulfoxide; Hydrolysis; Kinetics; Methylamines; Phenylalanine; Sodium Chloride; Solvents; Thermodynamics; Urea; Water | 2018 |
Uncovering metastability and disassembly hotspots in whole viral particles.
Topics: Chymotrypsin; Hydrogen Deuterium Exchange-Mass Spectrometry; Models, Molecular; Protein Conformation; Protein Unfolding; Proteolysis; Urea; Viral Proteins; Virion | 2019 |
Combined co-solvent and pressure effect on kinetics of a peptide hydrolysis: an activity-based approach.
Topics: Chymotrypsin; Dimethyl Sulfoxide; Hydrolysis; Kinetics; Methylamines; Phenylalanine; Pressure; Solvents; Thermodynamics; Urea; Water | 2019 |