uniconazole-p and kaurenoic-acid

uniconazole-p has been researched along with kaurenoic-acid* in 1 studies

Other Studies

1 other study(ies) available for uniconazole-p and kaurenoic-acid

Article	Year
The CYP701B1 of Physcomitrella patens is an ent-kaurene oxidase that resists inhibition by uniconazole-P. FEBS letters, 2011, Jun-23, Volume: 585, Issue:12 The moss Physcomitrella patens produces both ent-kaurene and ent-kaurenoic acid, which are intermediates of gibberellin biosynthesis in flowering plants. The CYP701 superfamily of cytochrome P450s functions as ent-kaurene oxidases in the biosynthesis of ent-kaurenoic acid. A candidate gene encoding ent-kaurene oxidase in P. patens, CYP701B1, was cloned and heterologously expressed in yeast to examine enzyme activities in vitro. The recombinant CYP701B1 protein catalyzed the oxidation reaction from ent-kaurene to ent-kaurenoic acid. CYP701B1 activity was highly resistant to the ent-kaurene oxidase inhibitor uniconazole-P (IC(50) 64 μM), even though the activity of Arabidopsis ent-kaurene oxidase (CYP701A3) was sensitive (IC(50) 0.26 μM). Topics: Arabidopsis; Bryopsida; Cytochrome P-450 Enzyme Inhibitors; Cytochrome P-450 Enzyme System; Diterpenes; Diterpenes, Kaurane; Gibberellins; Plant Proteins; Triazoles	2011

Article

Year

The CYP701B1 of Physcomitrella patens is an ent-kaurene oxidase that resists inhibition by uniconazole-P.

FEBS letters, 2011, Jun-23, Volume: 585, Issue:12

The moss Physcomitrella patens produces both ent-kaurene and ent-kaurenoic acid, which are intermediates of gibberellin biosynthesis in flowering plants. The CYP701 superfamily of cytochrome P450s functions as ent-kaurene oxidases in the biosynthesis of ent-kaurenoic acid. A candidate gene encoding ent-kaurene oxidase in P. patens, CYP701B1, was cloned and heterologously expressed in yeast to examine enzyme activities in vitro. The recombinant CYP701B1 protein catalyzed the oxidation reaction from ent-kaurene to ent-kaurenoic acid. CYP701B1 activity was highly resistant to the ent-kaurene oxidase inhibitor uniconazole-P (IC(50) 64 μM), even though the activity of Arabidopsis ent-kaurene oxidase (CYP701A3) was sensitive (IC(50) 0.26 μM).

Topics: Arabidopsis; Bryopsida; Cytochrome P-450 Enzyme Inhibitors; Cytochrome P-450 Enzyme System; Diterpenes; Diterpenes, Kaurane; Gibberellins; Plant Proteins; Triazoles

2011